1. Optimization of an O2-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment.
- Author
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Mouré, Anne, Bekir, Sawsen, Bacou, Elodie, Pruvost, Quentin, Haurogné, Karine, Allard, Marie, De Beaurepaire, Laurence, Bosch, Steffi, Riochet, David, Gauthier, Olivier, Blancho, Gilles, Soulillou, Jean-Paul, Poncelet, Denis, Mignot, Grégoire, Courcoux, Philippe, Jegou, Dominique, Bach, Jean-Marie, and Mosser, Mathilde
- Subjects
TYPE 1 diabetes ,ISLANDS of Langerhans ,EXPERIMENTAL design ,ARTIFICIAL pancreases ,RESPONSE surfaces (Statistics) ,PANCREAS ,ISLANDS - Abstract
A bioartificial pancreas (BAP) encapsulating high pancreatic islets concentration is a promising alternative for type 1 diabetes therapy. However, the main limitation of this approach is O
2 supply, especially until graft neovascularization. Here, we described a methodology to design an optimal O2 -balanced BAP using statistical design of experiment (DoE). A full factorial DoE was first performed to screen two O2 -technologies on their ability to preserve pseudo-islet viability and function under hypoxia and normoxia. Then, response surface methodology was used to define the optimal O2 -carrier and islet seeding concentrations to maximize the number of viable pseudo-islets in the BAP containing an O2 -generator under hypoxia. Monitoring of viability, function and maturation of neonatal pig islets for 15 days in vitro demonstrated the efficiency of the optimal O2 -balanced BAP. The findings should allow the design of a more realistic BAP for humans with high islets concentration by maintaining the O2 balance in the device. [ABSTRACT FROM AUTHOR]- Published
- 2022
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