Back to Search
Start Over
Optimization of an O2-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment.
- Source :
- Scientific Reports; 3/18/2022, Vol. 12 Issue 1, p1-17, 17p
- Publication Year :
- 2022
-
Abstract
- A bioartificial pancreas (BAP) encapsulating high pancreatic islets concentration is a promising alternative for type 1 diabetes therapy. However, the main limitation of this approach is O<subscript>2</subscript> supply, especially until graft neovascularization. Here, we described a methodology to design an optimal O<subscript>2</subscript>-balanced BAP using statistical design of experiment (DoE). A full factorial DoE was first performed to screen two O<subscript>2</subscript>-technologies on their ability to preserve pseudo-islet viability and function under hypoxia and normoxia. Then, response surface methodology was used to define the optimal O<subscript>2</subscript>-carrier and islet seeding concentrations to maximize the number of viable pseudo-islets in the BAP containing an O<subscript>2</subscript>-generator under hypoxia. Monitoring of viability, function and maturation of neonatal pig islets for 15 days in vitro demonstrated the efficiency of the optimal O<subscript>2</subscript>-balanced BAP. The findings should allow the design of a more realistic BAP for humans with high islets concentration by maintaining the O<subscript>2</subscript> balance in the device. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 12
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Scientific Reports
- Publication Type :
- Academic Journal
- Accession number :
- 155872538
- Full Text :
- https://doi.org/10.1038/s41598-022-07887-w