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Fibered fluorescence microscopy is a recent developed image modality using a fiber optic probe connected to a laser scanning unit. It allows for in-vivo scanning of small animal subjects by moving the probe along the tissue surface. During the scans images are continuity captured, allowing to acquire an area larger then the field of view of the probe as a video. But there is still a need to obtain a single static image from the multiple overlapping frames. In this paper we introduce a mosaicing procedure for this kind of video sequence. An additional motivation for the mosaicing is the use of overlapping redundant information to improve the signal to noise level of the acquisition, since the individual frames tend to have both high noise levels and intensity inhomogeneities.

The cell membrane consisting of the phospholipid bylayer and many different glycoproteins embedded in it is suspected to show a highly dynamic yet regulated behaviour. Impressive reorganizations are known to occur in particular in relation to cell movement and differentiation. While some components may move about relatively freely, local constraints and interactions with underlying cytomatrix elements are suspected to exist while others such as adhesion molecules may become immobilized at specific sites.

Many cells assume various polarized shapes in vivo depending on their genetic program and their interaction with the environment consisting of neighbouring cells, intercellular matrix elements and signal molecules. Some of these processes can be reproduced in cell culture systems. The adoption of an asymmetric polarized cell shape involves active deformation and motility. This is generated by the interaction of cytoplasmic force generating molecules with membrane embedded components which transmit the forces onto the extracellular matrix. Order and polarity is also displayed within the cells interior by the non-random distribution of various organelles such as Golgi elements, lysosomes, mitochondria etc. This is predominantly dependent on dynamic interactions between the membrane-lined organelles and the microtubule system. The intracellular polarity is most often related to the polarity generated by the cells motile activity. Membrane components are exchanged continuously between the cell membrane and the intracellular compartments.