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Zooplankton play a crucial role in estuarine ecosystems by aiding nutrient cycling through trophic chains and contributing to large-scale water filtration. The present review highlights the zooplankton research conducted over the last decade (2014-2024) in the Indian Sundarbans, with a focus on research trends, species distribution, hydrological associations, and long-term monitoring and conservation strategies. The study reveals a surge in zooplankton research after 2019, with an emphasis on both small- and large-scale studies. However, tidal creek/canals remain less explored compared to rivers. A lack of methodological standardization in zooplankton research is evident across the Indian Sundarbans. The Saptamukhi-Thakuran-Matla river stretch exhibited the highest species diversity, with 70 species recorded. Similarly, the tidal creeks and canals of Sagar Island were identified as species-rich habitats, with 63 species reported. Oithona brevicornis was found in seven riverine stretches, while Paracalanus parvus and Labidocera euchaeta were each recorded from six rivers, but Bestiolina similis was mostly reported as the numerically predominant zooplankton species. Salinity emerged as the most influential hydrological factor for zooplankton distribution, followed by dissolved oxygen and water temperature. However, increasing pollution, climate change-induced cyclones, salinization, and human activities are threatening zooplankton communities of the Indian Sundarbans. Furthermore, the review underscores the need for long-term monitoring strategies in the Sundarbans, by addressing the integration of remote sensing method, automated devices, data repositories, and ecological modeling approaches. Additionally, the present review recommends future policies for zooplankton conservation, emphasizing habitat protection, water quality assessment, stakeholder engagement, and securing funding to implement long-term monitoring initiatives., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2025. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

The global burden of cancer as a major cause of death and invalidity has been constantly increasing in the past decades. Monoamine oxidases (MAO) with two isoforms, MAO-A and MAO-B, are mammalian mitochondrial enzymes responsible for the oxidative deamination of neurotransmitters and amines in the central nervous system and peripheral tissues with the constant generation of hydrogen peroxide as the main deleterious ancillary product. However, given the complexity of cancer biology, MAO involvement in tumorigenesis is multifaceted with different tumors displaying either an increased or decreased MAO profile. MAO inhibitors are currently approved for the treatment of neurodegenerative diseases (mainly, Parkinson's disease) and as secondary/adjunctive therapeutic options for the treatment of major depression. Herein, we review the literature characterizing MAO's involvement and the putative role of MAO inhibitors in several malignancies, and also provide perspectives regarding the potential biomarker role that MAO could play in the future in oncology., Competing Interests: Declarations. Conflict of interest: Danina M. Muntean is an Associate Editor at Molecular and Cellular Biochemistry. Adrian Sturza is a member of the Editorial Board of the same journal. Apart from the above-mentioned statements, the authors have no relevant financial or non-financial interests to disclose. Ethical approval: Not applicable. Informed consent: Not applicable., (© 2024. The Author(s).)

In Salmonella Typhimurium, efflux pump proteins, such as AcrD actively expel drugs and hazardous chemicals from bacterial cells, resulting in treatment failure and the emergence of antibiotic-resistant variants. Focusing on AcrD may lead to the development of novel antimicrobials against multidrug-resistant bacteria. However, challenges persist in achieving high selectivity, low toxicity, and effective bacterial penetration. Natural products, particularly microbial secondary metabolites, possess distinct chemical structures that may target the efflux pump systems. The efflux pump inhibitor capabilities of Fusarium nygamai compounds in Salmonella have not been previously investigated. This study employed molecular docking and molecular dynamics simulations to evaluate 25 F. nygamai compounds as potential inhibitors of AcrD. Additionally, the pharmacological characteristics of these substances were examined. Molecular docking results revealed that 3,6-Dimethoxy-2,5-dinitrobenzonitrile, methyl (2-oxo-3-phenylquinoxalin-1(2H)-yl)acetate, and 7-Methyl-5-nitro-1,4-dihydro-quinoxaline-2,3-dione exhibited the highest binding energies with AcrD. Furthermore, molecular dynamics simulations indicated stable ligand-receptor complex variations over time. This study contributes to the efforts against antibiotic resistance and the improvement of Salmonella infection treatment outcomes globally by facilitating the development of novel therapeutic approaches and enhancing antibiotic efficacy., Competing Interests: Declarations. Conflicts of interest: The authors declare no potential conflicts of interest., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Alectinib, a second-generation anaplastic lymphoma kinase (ALK) inhibitor, has been shown to be effective for patients with ALK-positive non-small cell lung cancer (NSCLC). However, alectinib resistance is a serious problem worldwide. To the best of our knowledge, little information is available on its molecular mechanisms using the Gene Expression Omnibus (GEO) database. In this study, the differentially expressed genes (DEGs) were selected from the gene expression profile GSE73167 between parental and alectinib-resistant human lung adenocarcinoma (LUAD) cell samples. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) annotation enrichment analyses were conducted using Database for Annotation, Visualization and Integrated Discovery (DAVID). The construction of protein-protein interaction (PPI) network was performed to visualize DEGs. The hub genes were extracted based on the analysis of the PPI network using plug-in cytoHubba of Cytoscape software. The functional roles of the key genes were investigated using Gene Expression Profiling Interactive Analysis (GEPIA), University of Alabama at Birmingham Cancer (UALCAN), Gene Set Enrichment Analysis (GSEA), and Tumor Immune Estimation Resource (TIMER) analysis. The networks of kinase, miRNA, and transcription-factor targets of SFTPD were explored using LinkedOmics. The drug sensitivity analysis of SFTPD was analyzed using the RNAactDrug database. Results showed a total of 144 DEGs were identified. Five hub genes were extracted, including mucin 5B (MUC5B), surfactant protein D (SFTPD), deleted in malignant brain tumors 1 (DMBT1), surfactant protein A2 (SFTPA2), and trefoil factor 3 (TFF3). The survival analysis using GEPIA displayed that low expression of SFTPD had a significantly negative effect on the prognosis of patients with LUAD. GSEA revealed that low expression of SFTPD was positively correlated with the pathways associated with drug resistance, such as DNA replication, cell cycle, drug metabolism, and DNA damage repair, including mismatch repair (MMR), base excision repair (BER), homologous recombination (HR), and nucleotide excision repair (NER). The SFTPD expression was negatively correlated with the drug sensitivity of alectinib according to RNAactDrug database. The expression of SFTPD was further validated in parental H3122 cells and alectinib-resistant H3122 cells by quantitative reverse transcription PCR (RT-qPCR). In conclusion, our study found that the five hub genes, especially low expression of SFTPD, are closely related to alectinib resistance in patients with LUAD., Competing Interests: Declarations Competing interests NM, DBL, and DLW are employees of ChosenMed Technology. The remaining authors declare that they have no conflicts of interest. Ethical Approval and Consent to Participate Not applicable (GEO is an open database with ethical permission obtained for patients who participated. The data can be downloaded for free for research and publication. Open-source data are used in our research, so there are no ethical concerns). Consent for Publication Not applicable., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Plastic pollutants create health crises like physical damage to tissues, upset reproductive processes, altered behaviour, oxidative stress, neurological disorders, DNA damage, gene expression, and disrupt physiological functions, as the biosphere accumulates them inadvertently through the food web. Water resources have become the generic host of plastic wastes irrespective of their particle size, resulting in widespread distribution in aquatic environments. The pre-treatment step of the traditional water treatment process can easily remove coarse-sized plastic wastes. However, the fine plastic particles, with sizes ranging from nanometres to millimetres, are indifferent to the traditional water treatment. To address the escalating problems, the upgradation of different traditional physical, chemical, and biological remediation techniques offers a promising avenue for tackling tiny plastic particles from the water environment. Further, new techniques and hybrid incorporations to the existing water treatment techniques have been explored, specifically removing tiny plastic debris. A detailed understanding of the sources, fate, and impact of plastic wastes in the environment, as well as an evaluation of the above treatment techniques and their limitations and challenges, can only show the way for their upgradation, hybridization, and development of new techniques. This review paper provides a comprehensive overview of the current knowledge and techniques for the remediation of nanoplastics and microplastics., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Groundwater is an essential natural resource for mankind. Due to various geogenic and anthropogenic causes, groundwater quality has raised serious concern over the years. In this study, groundwater quality was evaluated for its suitability for irrigation in the Jorhat and Golaghat Districts of Assam, India. A total of 100 groundwater samples were collected from shallow aquifers (< 35 m) from different locations during the pre-monsoon season (March-April 2022). Groundwater in the study area is slightly alkaline in nature (mean pH value of 7.44). The average cations and anions chemistry are in the order of Na +  > Ca 2+ > Mg 2+  > K + and HCO 3 -  > Cl -  > SO 4 2-  > CO 3 2- , respectively. Ca-Mg-HCO 3 followed by Na-Ca-HCO 3 -Cl are the primary water types in the study area. Pearson's correlation matrix showed a positive correlation between TDS and EC (r = 0.78) and sodium showed a positive correlation with TDS and bicarbonate (r = 0.62 and r = 0.65), respectively. Gibbs plot indicated that rock-water interaction is the dominant factor that controls the chemistry of the groundwater of the area. Irrigation parameters like Sodium Absorption Ratio (SAR), Permeability Index (PI), Magnesium Absorption Ratio (MAR), Kelly's Ratio (KR), and Irrigation Water Quality Index (IWQI) indicated that groundwater is overall suitable for irrigation. USSL diagram illustrated that most of the samples fell into the C2 (medium salinity) and S1 (low sodium hazard) categories. Wilcox plot showed the samples fell in excellent to good categories indicating fitness of groundwater for irrigation in the area., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Purpose: The aim of the current study was to investigate the mtDNA methylation levels and mtDNA copy numbers in the sperm of patients with asthenozoospermia and compare them to those observed in controls with normozoospermia., Methods: Pyrosequencing analysis of the methylation levels of the mitochondrial D-loop and MT-CO1/chr1:631,907-632083/chrX:26,471,887-126,472,063 (hereinafter referred to as "MT-CO1-AVG") region and quantitative PCR analysis of the mtDNA copy number were performed on sperm from 30 patients with asthenozoospermia and 30 controls with normozoospermia., Results: Compared with those of controls with normozoospermia, the methylation levels of D-loop and MT-CO1-AVG regions and mtDNA copy number were significantly higher in patients with asthenozoospermia. The methylation level of the D-loop region in patients with asthenozoospermia and controls with normozoospermia and that of MT-CO1-AVG region in patients with asthenozoospermia showed a decreasing tendency with increasing total sperm motility. A significant inverse correlation between the mtDNA copy number and total sperm motility was observed in patients with asthenozoospermia but not in controls with normozoospermia. In patients with asthenozoospermia, but not in controls with normozoospermia, we observed a significant inverse correlation between D-loop methylation levels and mtDNA copy number, while no significant correlation was observed between MT-CO1-AVG methylation levels and mtDNA copy number., Conclusion: These results reveal the occurrence of mtDNA methylation in human sperm and altered D-loop and MT-CO1-AVG methylation levels in patients with asthenozoospermia. Additional research is needed to determine the function of these features in the etiology and course of asthenozoospermia., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Some recent studies highlight that vehicular traffic and honking contribute to more than 50% of noise pollution in urban or sub-urban areas in developing countries, including Indian cities. Frequent honking has an adverse effect on health and hampers road safety, the environment, etc. Therefore, recognizing the various vehicle honks and classifying the honk of different vehicles can provide good insights into environmental noise pollution. Moreover, classifying honks based on vehicle types allows for the inference of contextual information of a location, area, or traffic. So far, the researchers have done outdoor sound classification and honk detection, where vehicular honks are collected in a controlled environment or in the absence of ambient noise. Such classification models fail to classify honk based on vehicle types. Therefore, it becomes imperative to design a system that can detect and classify honks of different types of vehicles to infer some contextual information. This paper presents a novel framework A C lassi H onk that performs raw vehicular honk sensing, data labeling, and classifies the honk into three major groups, i.e., light-weight vehicles, medium-weight vehicles, and heavy-weight vehicles. Raw audio samples of different vehicular honking are collected based on spatio-temporal characteristics and converted them into spectrogram images. A deep learning-based multi-label autoencoder model (MAE) is proposed for automated labeling of the unlabeled data samples, which provides 97.64% accuracy in contrast to existing deep learning-based data labeling methods. Further, various pre-trained models, namely Inception V3, ResNet50, MobileNet, and ShuffleNet are used and proposed an Ensembled Transfer Learning model (EnTL) for vehicle honks classification and performed comparative analysis. Results reveal that EnTL exhibits the best performance compared to pre-trained models and achieves 96.72% accuracy in our dataset. In addition, context of a location is identified based on these classified honk signatures in a city., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Chemical and isotopic indicators were used to recognize the origin of hydrothermal groundwater, to assess the mineralization processes and groundwater quality, to identify the source of solutes and the likely mixing with cold, and elucidate the fluid geothermometry in the Jérid field of Southern Tunisia. The results show that the geothermal groundwater is neutral to slightly alkaline. They are characterized by SO 4 -Cl-Na-Ca water type. The dissolution of evaporates and pyrite-bearing rocks is the dominant mineralization process. The groundwater quality index indicates that the majority of samples are very hard and belong to poor to unsuitable for drinking classes. Applications and calculations of hydrogeochemical parameters, including SAR, %Na, PI, Kr, and MAR, showed that the majority of samples are unsuitable for agricultural practices. The human health risk was assessed based on hazard quotient and total hazard index through ingestion and dermal contact with iron-rich groundwater. The consumption of CI groundwaters does not present non-carcinogenic risk to adults and children. The δ 18 O and δ 2 H signatures indicate that the geothermal groundwater was recharged by ocean precipitation during cold and wet paleoclimatic periods. The slight enrichment of oxygen-18 and deuterium contents suggests a limited mixing effect between geothermal water and cold groundwater within the same aquifer. This mixing effect is confirmed by the Na-K-Mg and the chloride-enthalpy diagrams. The K-Mg and SiO 2 geothermometers provided fairly reliable reservoir temperature values, ranging between 69.6 and 99 °C. Calculated geothermal potential values, varying between 469 and 16987 kWth, which allow several applications such as domestic and agricultural heating., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

In the present study, the potential of Raman spectroscopy (RS) in predicting disease-free survival (DFS) in oral cancer patients has been explored. Raman spectra were obtained from the tumor and contralateral regions of 94 oral squamous cell carcinoma patients. These patients were managed surgically and recommended for adjuvant therapy. The Cox proportional survival analysis was carried out to identify the spectral regions that can be correlated to DFS. The survival analysis was performed with 95% confidence intervals, hazard ratio, and p-values in the 1200-1800 cm -1 spectral region. Out of a total of 182 spectral points, 76 were found to be correlating with DFS, suggesting their utility to predict the patient outcome. The cut-off points of each correlating RS-point values were defined and tested towards predicting the DFS. The performance of predicting the power of spectral points was validated through Brier value, and it was found to be closer to the actual progression. The 76 spectral points identified from the tumors have the potential to accurately predict DFS in oral squamous cell carcinoma through a relatively simplistic prediction model in the absence of confounding factors., (© 2021. The Author(s), under exclusive licence to Springer-Verlag London Ltd. part of Springer Nature.)

Plasmodium falciparum (P. falciparum), which causes the most severe form of malaria, if left untreated, has 24 h window in which it can cause severe illness and even death. The aim of this study was to create the most comprehensive and informative secretory-proteome possible by combining high-accuracy and high-sensitivity protein identification technology. In this study, we used Plasmodium falciparum 3D7 (Pf3D7) as the model parasite to develop a label-free quantification proteomic strategy with the main goal of identifying Pf3D7 proteins that are supposed to be secreted outside the infected erythrocytes in the spent media culture during the in-vitro study. The spent culture media supernatant was subjected to differential and ultra-centrifugation steps followed by total protein extraction, estimation, and in-solution digestion using trypsin, digested peptides were analyzed using Nano-LC coupled with ESI for MS/MS. MS/MS spectra were processed using Maxquant software (v2.1.4.0.). Non-infected erythrocytes incubated spent cultured media supernatant were considered as control. Out of discovered 38 proteins, proteins belonging to P. falciparum spp. were EGF-like protein (C0H544), Endoplasmic reticulum chaperone GRP170 (C0H5H0), Small GTP-binding protein sar1 (Q8I1S0), Erythrocyte membrane protein 1, PfEMP1 (Q8I639), aldehyde reductase (Q8ID61), Conserved Plasmodium proteins (Q8IEH3, Q8ILD1), Antigen 332, DBL-like protein (Q8IHN4), Fe-S cluster assembly protein (Q8II78), identified and chosen for further in-depth investigation. This study highlights the value of secretory Plasmodium proteins play crucial roles in various aspects of the disease progression and host-pathogen interactions which can serve as diagnostic markers for malaria infection., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

While mitochondria are susceptible to environmental detriments, little is known about potential associations between arsenic metabolites and mitochondria DNA copy number (mtDNAcn). We attempted to examine whether maternal urinary arsenic metabolite levels in different trimesters were related to neonatal cord blood mtDNAcn. We included 819 mother-newborn pairs embedded in an in-progress birth cohort survey performed from April 2014 to October 2016 in Wuhan, China. We determined maternal urinary arsenic species concentrations in different trimesters. We determined cord blood mtDNAcn using quantitative real-time polymerase chain reaction. In covariate-adjusted models, each one-unit increment of dimethylated arsenic (DMA) and total arsenic (TAs) in the third trimester was related to 8.43% (95% CI 1.13%, 16.26%) and 12.15% (95% CI 4.35%, 20.53%) increases in mtDNAcn, respectively. The dose-response trend with statistical significance was observed across tertiles of DMA and TAs in the third trimester with mtDNAcn (DMA percent changes (%Δ) = 25.60 (95% CI 6.73, 47.82), for the highest vs the lowest tertile (P = 0.02); TAs %Δ = 40.31 (95% CI 19.25, 65.10), for the highest vs the lowest tertile (P = 0.0002)). These findings may prove the relationships between prenatal arsenic species levels and neonatal mitochondrial dysfunction., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Obesity is a weight-related disorder characterized by excessive adipose tissue growth and dysfunction which leads to the onset of a systemic chronic low-grade inflammatory state. Likewise, inflammation is considered a classic cancer hallmark affecting several steps of carcinogenesis and tumor progression. In this regard, novel molecular complexes termed inflammasomes have been identified which are able to react to a wide spectrum of insults, impacting several metabolic-related disorders, but their contribution to cancer biology remains unclear. In this context, prostate cancer (PCa) has a markedly inflammatory component, and patients frequently are elderly individuals who exhibit weight-related disorders, being obesity the most prevalent condition. Therefore, inflammation, and specifically, inflammasome complexes, could be crucial players in the interplay between PCa and metabolic disorders. In this review, we will: 1) discuss the potential role of each inflammasome component (sensor, molecular adaptor, and targets) in PCa pathophysiology, placing special emphasis on IL-1β/NF-kB pathway and ROS and hypoxia influence; 2) explore the association between inflammasomes and obesity, and how these molecular complexes could act as the cornerstone between the obesity and PCa; and, 3) compile current clinical trials regarding inflammasome targeting, providing some insights about their potential use in the clinical practice., (© 2023. The Author(s).)

Background: Lactate is an important metabolite derived from glycolysis under physiological and pathological conditions. The Warburg effect reveals the vital role of lactate in cancer progression. Numerous studies have reported crucial roles for lactate in cancer progression and cell fate determination. Lactylation, a novel posttranslational modification (PTM), has provided a new opportunity to investigate metabolic epigenetic regulation, and studies of this process have been initiated in a wide range of cancer cells, cancer-associated immune cells, and embryonic stem cells., Conclusion: Lactylation is a novel and interesting mechanism of lactate metabolism linked to metabolic rewiring and epigenetic remodeling. It is a potential and hopeful target for cancer therapy. Here, we summarize the discovery of lactylation, the mechanisms of site modification, and progress in research on nonhistone lactylation. We focus on the potential roles of lactylation in cancer progression and cell fate determination and the possible therapeutic strategies for targeting lysine lactylation. Finally, we suggest some future research topics on lactylation to inspire some interesting ideas., (© 2023. Springer Nature Switzerland AG.)

The reduction of income inequality and environmental frailty are important factors which can help achieve sustainable development. In this context, it is important to investigate the nexus between income inequality and carbon dioxide emission by considering the role of political stability. This paper examines the effect of political stability, economic growth, financial development, and carbon dioxide on income inequality in developing countries, high-income countries, and the Belt Road initiative (BRI) countries from 2002 to 2019. By employing a two-step generalized method of moments and panel quantile regression, the findings show that carbon dioxide emission, financial development, and political stability rise income inequality while economic growth significantly reduces income inequality in developing countries. In the case of high-income countries, political stability and carbon dioxide negatively affect income inequality while financial development rise income inequality. In the case of BRI countries, political stability, economic growth, and carbon dioxide emission significantly reduce income inequality. Our findings have considerable policy implications regarding reducing income inequality in the sample countries., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Natural and anthropogenic processes are primary drivers for soil degradations in the Gangetic plain of India. Indian Remote Sensing (IRS) data was used to assess soil degradations and study spatial changes comparing with the legacy datasets. Based on the nature, extent, and degrees of limitations, these soils were evaluated to suggest suitable reclamation and management options. Typical strong signature (white tones) from a barren highly salinized soil (P3, P6, P9, P11, and P15) and higher energy absorption (blue-black tones) from a permanent waterlogged surface (P13, P14, P10) in the irrigated areas have favored delineation of salt-affected, strong, and permanent waterlogged areas. Moderate and slightly salt-affected soils (P1, P2, P4, P5, P7, P8, and P12) and the areas with high water table depths (P13 and P14) have shown mixed spectral signatures for associations of soil, water, and crops and were mapped using ground truth and soil chemical analysis data. Irrigation with poor quality ground water (RSC: 1.2 to 12.3 me L -1 ) has prompted developing salinity and sodicity in P2, P5, and P11, while irrigation in poorly drained areas caused appearance of waterlogging (WTD < 1.5 m) and salinization (P3, P5, P7, P9, P13, P14). The gypsum application and sub-surface drainage (SSD) techniques have enhanced soil reclamation by (i) neutralizing CO 3 2  - and HCO 3  - in P12 (pH 9.2, ESP 24) and (ii) controlling soil salinity (EC 3.0 dS m -1 ) and waterlogging (WTD > 1.5 m) in P14. Soils with CaCO 3 layers at shallow depth in P3 (0.7-4.2%), P6 (1.2-7.1%), P9 (8-16%), P11 (4-15%), and P13 (0.9-9.4%) were managed using forest plantations. Ground water with high SAR in T1 to T7 can be applied for agriculture using mixing or cyclic mode. Water with high RSC in T4, T5, T7, T8, T9, and T10 needs treatment with gypsum. A significant area (~ 24.87%) of salt-affected soils was reclaimed since 1971. Prominent changes (3.27 to 17.71%) were shown in Panipat and Karnal districts. Small areas of brick kiln (P11), industrial effluent (P10), riverine sand, partially stabilized dunes, and mining have appeared due to anthropogenic activities., (© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

The upper Brahmaputra floodplain is considerably enriched with geogenic contaminants, mainly Arsenic (As) exhibiting high spatial heterogeneity. With this concern, the present study was conducted in Lakhimpur district located in the upper Brahmaputra floodplain aiming to assess the groundwater (GW) suitability for drinking and irrigation. Chemical analysis of 78 GW samples revealed significantly high As (max 0.12 mgL -1 , mean 0.02 mgL -1 ) followed by iron (max 62.2 mgL -1 , mean 19.94 mgL -1 ) showing uneven distribution. Hydrogeochemical analysis of the GW samples revealed that majority of the water samples belong to mixed Ca 2+ - Na + - HCO 3 - type with weathering and forward ion exchange as the dominant processes in the aquifer system. Positive correlation among iron (Fe) and As, together with close grouping in hierarchical cluster analysis and principal components analysis, indicated the involvement of reductive hydrolysis process. Calculation of saturation indices indicated that, precipitation of Fe minerals may lead to de-coupling of Fe and As contributing to the enrichment of both the elements in the GW. Further, assessment of key parameters for irrigation water quality including water quality index indicated the GW to be suitable for irrigation purpose. Finally, toxicity implications of crops produced from As contaminated water indicated higher accumulation potential of As in the food grains implying significant impacts on the agro-ecosystem and associated health hazards., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Cleft lip and palate is one of the most frequent congenital developmental defects. Autophagy is a highly conserved process of cell self-degradation in eukaryotes, involving multiple biological processes in which chloroquine (CQ) is the most common inhibitor. However, whether CQ affects and how it affects palate development is unknown. Mouse embryonic palatal cells (MEPCs) were treated with CQ to observe cell viability, apoptosis, migration, osteogenic differentiation by cell proliferation assay, flow cytometric analysis, scratch assay, and alizarin red staining. PI staining was used to measure cell cycle distribution. Immunofluorescence (IF) assay and transmission electron microscopy were used to detect autophagosomes. The autophagy-related factors (LC3 and P62), apoptosis-related markers (P53, caspase-3 cleaved caspase-3, BAX, and BCL-2), and cell cycle-related proteins (P21, CDK2, CDK4, cyclin D1, and cyclin E) were all measured by western blot. CQ inhibited the proliferation of MEPCs by arresting the G0/G1 phase of the cell cycle in a concentration- and time-dependent manner with cell cycle-related proteins P21 upregulated and CDK2, CDK4, cyclin D1, and cyclin E downregulated. Then we detected CQ also induced cell apoptosis in a dose-dependent manner by decreasing the BCL-2/BAX ratio and increasing cleaved caspase-3. Next, it was investigated that migration and osteogenesis of MEPCs decreased with CQ treatment in a dose-dependent manner. Meanwhile, CQ blocked the autophagy pathway by upregulating LC3II and P62 expressions which activated the P53 pathway. CQ activates P53 which affects MEPC biological characteristics by changing the proliferation and apoptosis of MEPCs through inhibiting autophagy., (© 2022. The Society for In Vitro Biology.)

The Ganga River is the major source of drinking water for humans over the decades. It is also the ecological niche for millions of relict species, i.e., for a variety of planktons, benthic organisms, fish, and various other aquatic organisms. The blasting population resulted in an enhanced rate of pollution in the river system emanating from various anthropogenic activities and industrialization in the bank of river Ganga. The study was made in the middle and lower stretch of the river to monitor the decadal changes in the water quality of river Ganga from 1960 to 2019 at six different study sites. In the present study, various water quality parameters such as dissolved oxygen, pH, free carbon dioxide, total alkalinity, conductivity, total dissolved solids (TDS), hardness, chloride, and nitrate have been studied during 2015-2019. The data for 1960 to 2006 were taken from ICAR-CIFRI publications. Based on the studied parameters, National Sanitation Foundation (NSF)-water quality index (WQI) was calculated. In the present study, it was found that the calculated NSF-WQI was 69.24 in 1960-1961 which increased up to 113.39 during 2001-2006. But, with the implementation of various rejuvenating strategies, the WQI of the river got reduced to 106.48 during 2015-2019. This reflected the positive changes in the riverine system. Different water quality parameters such as dissolved oxygen, pH, and hardness were observed mostly within the permissible range as based on the drinking water guidelines for humans and survival of the aquatic organisms as well, except a few location-specific observations., (© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Background: Brain-to-brain evoked potentials constitute a new methodology that could help to understand the network-level correlates of electrical stimulation applied for brain mapping during tumor resection. In this paper, we aimed to describe the characteristics of axono-cortical evoked potentials recorded from distinct, but in the same patient, behaviorally eloquent white matter sites., Methods: We report the intraoperative white matter mapping and axono-cortical evoked potentials recordings observed in a patient operated on under awake condition of a diffuse low-grade glioma in the left middle frontal gyrus. Out of the eight behaviorally eloquent sites identified with 60-Hz electrical stimulation, five were probed with single electrical pulses (delivered at 1 Hz), while recording evoked potentials on two electrodes, covering the inferior frontal gyrus and the precentral gyrus, respectively. Postoperative diffusion-weighted MRI was used to reconstruct the tractograms passing through each of the five stimulated sites., Results: Each stimulated site generated an ACEP on at least one of the recorded electrode contacts. The whole pattern-i.e., the specific contacts with ACEPs and their waveform-was distinct for each of the five stimulated sites., Conclusions: We found that the patterns of ACEPs provided unique electrophysiological signatures for each of the five white matter functional sites. Our results could ultimately provide neurosurgeons with a new tool of intraoperative electrophysiologically based functional guidance., (© 2021. Springer-Verlag GmbH Austria, part of Springer Nature.)

Classical swine fever (CSF) is a highly contagious dreadful disease of pigs leading to 100% mortality in acute form in susceptible population thereby causing huge economic loss to pig farmers. This study was undertaken to assess the seroprevalence of CSF at national level. A two-stage random sampling methodology was adopted that included 271 villages from 115 districts of India. A total of 5848 pig serum samples from twenty-five states and one Union Territory of India were collected during 2018-2019. A percent positivity of 38.52 was found at national level. Puducherry and Sikkim showed the highest and lowest percent positivity respectively. Pigs from the west zone showed the highest seroprevalence of 55.83% and those from the south zone showed the lowest of 30.25%. Adult pigs in the north and east zones showed highest percent positivity of 81.8, whereas pigs of more than 3 years of age showed highest percent positivity of 54.9, 75 and 62.5 in the north east, west and central zones respectively. Young ones showed percent positivity of 41.5 in the south zone. Higher rainfall (> 3 mm/day) and lower temperature (< 26 °C) favoured the existence of disease in the north east region combined with high density of pig population. Amidst no fool proof alert system, seroprevalence is the best method to assess the status of CSF in herd/population that provides the policymakers to plan for control of disease.

Marine phycosphere harbors unique cross-kingdom associations with enormous ecological significance in aquatic ecosystems as well as relevance for algal biotechnology industry. During our investigating the microbial composition and bioactivity of marine phycosphere microbiota (PM), a novel lightly yellowish and versatile bacterium designated strain AM1-D1 T was isolated from cultivable PM of marine dinoflagellate Alexandrium minutum amtk4 that produces high levels of paralytic shellfish poisoning toxins (PSTs). Strain AM1-D1 T demonstrates notable bioflocculanting bioactivity with bacterial exopolysaccharides (EPS), and microalgae growth-promoting (MGP) potential toward its algal host. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AM1-D1 T was affiliated to the members of genus Sulfitobacter within the family Rhodobacteraceae, showing the highest sequence similarity of 97.9% with Sulfitobacter noctilucae NB-68 T , and below 97.8% with other type strains. The complete genome of strain AM1-D1 T consisted of a circular 3.84-Mb chromosome and five circular plasmids (185, 95, 15, 205 and 348 Kb, respectively) with the G+C content of 64.6%. Low values obtained by phylogenomic calculations on the average nucleotide identity (ANI, 77.2%), average amino acid identity (AAI, 74.7%) and digital DNA-DNA hybridization (dDDH, 18.6%) unequivocally separated strain AM1-D1 T from its closest relative. The main polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, one unidentified phospholipid and one unidentified lipid. The predominant fatty acids (> 10%) were C 18:1 ω7c, C 19:0 cyclo ω8c and C 16:0 . The respiratory quinone was Q-10. The genome of strain AM1-D1 T was predicted to encode series of gene clusters responsible for sulfur oxidation (sox) and utilization of dissolved organic sulfur exometabolites from marine dinoflagellates, taurine (tau) and dimethylsulfoniopropionate (DMSP) (dmd), as well as supplementary vitamin B 12 (cob), photosynthesis carotenoids (crt) which are pivotal components during algae-bacteria interactions. Based on the evidences by the polyphasic characterizations, strain AM1-D1 T represents a novel species of the genus Sulfitobacter, for which the name Sulfitobacter alexandrii sp. nov. is proposed. The type strain is AM1-D1 T (= CCTCC 2017277T = KCTC 62491 T ).

Background: Laparoscopic sleeve gastrectomy (LSG) is safe and effective in type 2 diabetes mellitus (T2DM) with body mass index (BMI) ≥ 35 kg/m 2 . However, the effect and safety of LSG in the treatment of T2DM patients with BMI less than 35 kg/m 2 , especially in patients with BMI less than 30 kg/m 2 , are still debatable., Methods: A total of 51 T2DM patients with BMI less than 35 kg/m 2 treated successfully with LSG were included in our study. All patients were divided into two groups for subgroup analysis, namely group A (BMI 23.23-29.97 kg/m 2 ) and group B (BMI 30.0-34.9 kg/m 2 ). The weight loss and diabetic characteristics of LSG in the two groups at 3 months, 6 months, and 12 months after operation were compared, respectively., Results: In group A, the complete remission rates of T2DM were 20%, 36%, and 44% at 3, 6, and 12 months after operation, respectively. In group B, the complete remission rates of T2DM were 30.8%, 61.5%, and 88.5% at 3, 6, and 12 months after operation, respectively. Besides, none of the patients with a duration more than 15 years achieved complete remission. For T2DM patients with BMI < 30 kg/m 2 at 12 months after LSG, the complete remission rate of T2DM was 15.4% in patients with ABCD scores ≤ 2, and the complete remission rate of T2DM was 100% in patients with ABCD scores ≥ 5., Conclusion: LSG is safe and effective for T2DM patients with BMI less than 35 kg/m 2 in the short-term. However, the complete remission rate of T2DM in patients with BMI less than 30 kg/m 2 is far from the diabetes patients with BMI at 30-34.9 kg/m 2 .

In this study, we sequenced the V3-V4 region of 16S rRNA gene amplicon using paired-end Illumina HiSeq to study the bacterial community in the gills of fish from the bank of the trans-border river of Brahmaputra, Northeast India. Metagenome data consisted of 278,784 reads, 248-bp length, and 56.48% GC content with 85% sequence having a Phred score Q = 30. Community metagenomics revealed a total of 631 genera belonging to 22 different phyla, dominated by Proteobacteria (118,222 features), Firmicutes (101,043 features), Actinobacteria (34,189 features), Bacteroidetes (17,977 features), and Cyanobacteria (2730 features). The bacterial community identified was composed of both pathogenic zoonotic and non-harmful groups. The pathway or functional analysis of the fish gill microbiome exhibited 21 different pathways which also included the pathogenic-related functions. Our data detected a wide group of bacterial communities that will be useful in further isolating and characterizing the pathogenic bacteria from the fish and also to understand the bacterial association in highly consumed fish.

Anthropogenic activities pose challenges on security of water quality. Identifying potential sources of pollution and quantifying their corresponding contributions are essential for water management and pollution control. In our study, 2-year (2017-2018) water quality dataset of 15 parameters from eight sampling sites in tributaries and mainstream of the Min River was analyzed with multivariate statistical analysis methods and absolute principal component score-multiple linear regression (APCS-MLR) receptor modeling technique to reveal potential sources of pollution and apportion their contributions. Temporal and spatial cluster analysis (CA) classified 12 months into three periods exactly consistent with dry, wet, and normal seasons, and eight monitoring sites into two regions, lightly polluted (LP) and highly polluted (HP) regions, based on different levels of pollution caused by physicochemical properties and anthropogenic activities. The principal component analysis (PCA) identified five latent factors accounting for 75.84% and 73.46% of the total variance in the LP and HP regions, respectively. The main pollution sources in the two regions included agricultural activities, domestic sewage, and industrial wastewater discharge. APCS-MLR results showed that in the LP region, contribution of five potential pollution sources was ranked as agricultural non-point source pollution (22.13%) > seasonal effect and phytoplankton growth (19.86%) > leakage of septic tanks (15.73%) > physicochemical effect (12.86%) > industrial effluents and domestic sewage (11.59%), while in the HP region ranked as point source pollution from domestic and industrial discharges (20.81%) > municipal sewage (16.66%) > agricultural non-point source pollution (15.23%) > phytoplankton growth (14.82%) > natural and seasonal effects (12.67%). Based on the quantitative assessment of main pollution sources, the study can help policymakers to formulate strategies to improve water quality in different regions.

Author(s): Douglas P. Kennedy [sup.1] , Alex Haley [sup.1] , Roni Evans [sup.1] Author Affiliations: (1) grid.17635.36, 0000000419368657, University of Minnesota, Earl E. Bakken Center for Spirituality & Healing, , [...], Addressing teachers&apos; ability to better navigate stress and emotional responses has the potential to improve classroom behaviors, student learning as well as teachers&apos; wellbeing. While there is growing interest in mindfulness-based interventions (MBIs), there is need for greater attention to design aligned with relevant theories and transparent reporting to improve the rigor and translation of research. While utilized widely in health and behavior fields, behavior change theory has not been applied extensively in education or MBI research. This descriptive methodological paper articulates the intervention mapping and design process of an MBI to improve teachers&apos; stress and emotional regulation. To improve reporting, we use a post hoc application of a well-established behavioral model. Application of the Behavior Change Wheel (BCW) as part of an intervention mapping approach for an MBI to align teacher&apos;s needs with intervention elements most likely to achieve desired behavioral outcomes. The paper illustrates an approach which includes defining the problem in behavioral terms, identification of target behaviors and behavioral change techniques, and selection of strategies and modes of intervention delivery. A systematic intervention mapping approach applying the BCW can yield a holistic MBI for the desired behavior change, enhanced emotion and stress regulation in teachers. This example of applying the BCW to the design of an MBI demonstrates how intervention designers and researchers can make decisions about which techniques and delivery components to include in their interventions, and how to systematically describe them. Such approaches have the potential to improve the reporting and methodological rigor of future MBI research and the successful application of interventions in practice.