1. Evaluating antimicrobial activity and cytotoxicity of silver nanoparticles incorporated into reinforced zinc oxide eugenol: an in vitro study.
- Author
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Shid-Moosavi TS, Mohammadi N, Gharamani Y, Motamedifar M, and Alizadeh AA
- Subjects
- Humans, Zinc Oxide-Eugenol Cement pharmacology, Zinc Oxide-Eugenol Cement toxicity, In Vitro Techniques, Anti-Infective Agents pharmacology, Dental Pulp drug effects, Dental Pulp cytology, Microbial Sensitivity Tests, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents toxicity, Cell Survival drug effects, Stem Cells drug effects, Materials Testing, Silver pharmacology, Metal Nanoparticles toxicity, Streptococcus mutans drug effects, Lactobacillus acidophilus drug effects
- Abstract
Purpose: This study aimed to evaluate the antibacterial and cytotoxic effects of reinforced zinc oxide-eugenol (rZOE) incorporated with different concentrations of silver nanoparticles (AgNPs)., Methods: The pastes of rZOE alone or mixed with AgNPs at concentrations of 1%, 2%, and 5% of weight were prepared. In vitro antimicrobial activity of prepared materials against Streptococcus (S.) mutans and Lactobacillus (L.) acidophilus were evaluated after 2, 4, and 6 h of contact times using direct contact test (DCT) and also following 24 h incubation by well-diffusion test (WDT). The cytotoxicity of the tested materials on human dental pulp stem cells was also determined by MTT assay., Results: The DCT demonstrated that the time-dependent reductions of the colony numbers of both bacteria by three different concentrations of AgNPs incorporated into rZOE were equal but steeper than the rZOE alone (P < 0.05). The increases in growth inhibition zones of S. mutans and L. acidophilus were associated with the increasing concentration of AgNPs mixed with rZOE in the WDT; however, statistical analysis did not show any significant differences (P = 0.092). The MTT assay revealed a significantly lower percentage of cell viability after 1 day of culture only with the rZOE + AgNP5% in comparison to the rZOE alone (P = 0.011) and the control medium (P = 0.001)., Conclusion: Since the antimicrobial activities of three different concentrations of AgNPs incorporated into rZOE were equal and AgNPs had lower toxicity at lower concentrations, using AgNPs at 1% concentration is suggested to be mixed with rZOE., (© 2024. The Author(s), under exclusive licence to European Academy of Paediatric Dentistry.)
- Published
- 2024
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