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Introduction: Biologic mediators such as platelet-rich fibrins (PRF) or enamel matrix derivatives (Emdogain®) are used for the regeneration of the soft and hard tissues. The present study aimed to compare the efficacy of PRF and Emdogain® on the proliferation and differentiation of gingival fibroblasts in vitro. Materials and Methods: In this randomized controlled in vitro trial, blood samples were obtained from a healthy person and Choukroun’s PRF was prepared according to the standard protocol. Fibroblast cells were cultured with PRF and different concentration of Emdogain®. MTT test were used to measure fibroblast cells proliferation after 24 and 72 hours. Results: As compared to negative control (FBS 1%), Emdogain® 50μg/ml significantly increased cells proliferation up to 123.2% and 111.2% after 24 and 72 hours. The values were 214.9% and 121.9% for Emdogain® 100μg/ml. The increased proliferation values were significant for both groups after 24 hours (P

Introduction: This study compared the root surface roughness following scaling and root planning with manual curettes and different powers of Er:YAG and Er,Cr:YSGG lasers using surface profilometry and scanning electron microscopy (SEM). Materials and Methods: In this in vitro experimental study, 50 extracted teeth were buccolingually sectioned into two halves. The obtained contaminated surfaces randomly received the following treatments: SRP with manual curettes (group I), Er:YAG laser irradiation (4 W) (group II), manual curette+Er: YAG laser (1W) (group III), manual curette+ Er,Cr:YSGG laser (150 mJ) (group IV) and Er,Cr:YSGG laser (250 mJ) (group V). Surface roughness (Ra), surface changes (Rz) and maximum roughness changes (Rmax) were calculated before and after treatment while the surface morphology was examined by SEM analysis. The differences in roughness parameters were statistically analyzed using Wilcoxon signed rank test for each modality. Results: Except for the manual curette group (I) in which the roughness parameters decreased significantly (P