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Comparison of Enamel Matrix Derivatives and Platelet-Rich Fibrin on Human Gingival Fibroblasts Proliferation and Viability
- Source :
- Regeneration, Reconstruction & Restoration, Vol 4, Iss 4, Pp 130-134 (2019)
- Publication Year :
- 2019
- Publisher :
- Shahid Beheshti University of Medical Sciences, 2019.
-
Abstract
- Introduction: Biologic mediators such as platelet-rich fibrins (PRF) or enamel matrix derivatives (Emdogain®) are used for the regeneration of the soft and hard tissues. The present study aimed to compare the efficacy of PRF and Emdogain® on the proliferation and differentiation of gingival fibroblasts in vitro. Materials and Methods: In this randomized controlled in vitro trial, blood samples were obtained from a healthy person and Choukroun’s PRF was prepared according to the standard protocol. Fibroblast cells were cultured with PRF and different concentration of Emdogain®. MTT test were used to measure fibroblast cells proliferation after 24 and 72 hours. Results: As compared to negative control (FBS 1%), Emdogain® 50μg/ml significantly increased cells proliferation up to 123.2% and 111.2% after 24 and 72 hours. The values were 214.9% and 121.9% for Emdogain® 100μg/ml. The increased proliferation values were significant for both groups after 24 hours (P
Details
- Language :
- English
- ISSN :
- 24765163 and 24765171
- Volume :
- 4
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- Regeneration, Reconstruction & Restoration
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.6d33c0f500b540d7aa5d30b354471b13
- Document Type :
- article
- Full Text :
- https://doi.org/10.22037/rrr.v4i4.29625