1. A vascularized nerve graft substitute generated in a chamber bioreactor- A preliminary report
- Author
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İbrahim Alper Aksakal, Bulent Ayas, Yener Demirtas, Ahmet Karacalar, Murat Sinan Engin, and Tale Neimetzade
- Subjects
cell culture ,Future studies ,business.industry ,Regeneration (biology) ,lcsh:Surgery ,Nerve graft ,Schwann cell ,vascularized nerve graft ,lcsh:RD1-811 ,Anatomy ,autologous schwann cells ,medicine.anatomical_structure ,In vivo ,Preliminary report ,tissue engineering ,medicine ,Bioreactor ,Sciatic nerve ,business ,Peripheral nerves ,Biomedical engineering - Abstract
Objectives: Treatments of long peripheral nerve defects are limited and a means to construct a vascularized nerve graft substitute has been sought. Material and Methods: An in vivo chamber bioreactor was designed with the White New Zealand rabbit's superficial inferior epigastric pedicle (SIEP) and using autologous Schwann cell (ASC) suspension, a tissue volume containing rich vascularization and a dense presence of Schwann cells was constructed. This construct was comparatively tried on 3 cm of a sciatic nerve defect. Results: A vascularized build with rich blood supply and an abundance of Schwann cells was engineered and utilized for nerve regeneration and transmission, axonal passage being displayed. Conclusion: According to the literature review, this is the first achievement of engineering a nerve graft substitute containing vascularized Schwann cells. Therefore, it is believed this study has yielded significant preliminary results, providing a basis for future studies. [Hand Microsurg 2016; 5(2.000): 62-69]
- Published
- 2016