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Multigroup latent profile analysis examined whether patterns of alcohol, cannabis, and tobacco use varied between college student and nonstudent young adults (N = 745), and profiles were characterized by alcohol- and drug-related problems, psychological distress, and perceived stress. Findings supported three profiles (high use across substances, primarily high tobacco use, and low use across substances) among students. Students in the high use group, relative to other groups, experienced the highest number of problems and psychological distress. Among nonstudents, two profiles emerged (high use across substances and primarily high tobacco use), and noncollege participants in the high use group experienced the highest problems, psychological distress, and perceived stress. Students, compared to nonstudents, reported more variability in their substance use profiles, as a low substance use group was not observed among nonstudents. Prevention and intervention efforts should target nonstudents, which could aid in reducing negative health outcomes based on disparities in education. [ABSTRACT FROM AUTHOR]

Dendrites are tree-like structures with tiny spines specialized to receive excitatory synaptic transmission. Spino-dendritic plasticity, driven by neural activity, underlies the maintenance of neuronal connections crucial for proper circuit function. Abnormalities in dendritic morphology are frequently seen in epilepsy. However, the exact etiology or functional implications are not yet known. Therefore, to better comprehend the structure-function significance of this dendritic pathology in epilepsy, it is necessary to identify the common spino-dendritic disturbances present in both human and experimental models. Here, we describe the dendritic and spine structural profiles found across human refractory epilepsy as well as in animal models of developmental, acquired, and genetic epilepsies. [ABSTRACT FROM AUTHOR]

Graphic facilitation is a creative, robust visual communication process and tool that can be used by researchers for several benefits including improving data integrity; mitigating barriers between researchers and participants; promoting participants' ownership of data, decision-making, and creativity; and, co-creating knowledge, which is of particular interest among certain cultures and in some contexts. For Indigenous Peoples who traditionally use visual, oral, and narrative modalities as primary forms of communication, graphic facilitation is a methodology that aligns well with these modes of communicating. In this article, we describe our use of graphic facilitation in a community-led project exploring Indigenous parents' perceptions of community strengths, needs and priorities related to healthy early childhood development and optimal parenting. In collaboration with the Indigenous Friendship Centre in Hamilton, Canada, we held a Community Gathering that was facilitated by a graphic artist experienced in working with the Indigenous community; the findings resulting from the Gathering are presented. We discuss how researchers can use graphic facilitation as a tool to ensure adherence to the OCAP® principles of data ownership, control, access, and possession for the Indigenous community and describe the potential for mitigating power imbalances. Finally, considerations for researchers contemplating using graphic facilitation as a tool for research projects with Indigenous people and communities are presented. [ABSTRACT FROM AUTHOR]

Selective Neuronal Knockout of STAT3 Function Inhibits Epilepsy Progression, Improves Cognition, and Restores Dysregulated Gene Networks in a Temporal Lobe Epilepsy Model Tipton AE, Del Angel YC, Hixson K, Carlsen J, Strode D, Busquet N, Mesches MH, Gonzalez MI, Napoli E, Russek SJ, Brooks-Kayal AR. Ann Neurol. 2023 Mar 19. doi:10.1002/ana.26644 Objective: Temporal lobe epilepsy (TLE) is a progressive disorder mediated by pathological changes in molecular cascades and hippocampal neural circuit remodeling that results in spontaneous seizures and cognitive dysfunction. Targeting these cascades may provide disease-modifying treatments for TLE patients. Janus Kinase/Signal Transducer and Activator of Transcription (JAK/STAT) inhibitors have emerged as potential disease-modifying therapies; a more detailed understanding of JAK/STAT participation in epileptogenic responses is required, however, to increase the therapeutic efficacy and reduce adverse effects associated with global inhibition. Methods: We developed a mouse line in which tamoxifen treatment conditionally abolishes STAT3 signaling from forebrain excitatory neurons (nSTAT3KO). Seizure frequency (continuous in vivo electroencephalography) and memory (contextual fear conditioning and motor learning) were analyzed in wild-type and nSTAT3KO mice after intrahippocampal kainate (IHKA) injection as a model of TLE. Hippocampal RNA was obtained 24 h after IHKA and subjected to deep sequencing. Results: Selective STAT3 knock-out in excitatory neurons reduced seizure progression and hippocampal memory deficits without reducing the extent of cell death or mossy fiber sprouting induced by IHKA injection. Gene expression was rescued in major networks associated with response to brain injury, neuronal plasticity, and learning and memory. We also provide the first evidence that neuronal STAT3 may directly influence brain inflammation. Interpretation: Inhibiting neuronal STAT3 signaling improved outcomes in an animal model of TLE, prevented progression of seizures and cognitive co-morbidities while rescuing pathogenic changes in gene expression of major networks associated with epileptogenesis. Specifically targeting neuronal STAT3 may be an effective disease-modifying strategy for TLE. [ABSTRACT FROM AUTHOR]

Hysteresis in motor planning and syntactic priming in language planning refer to the influence of prior production history on current production behaviour. Computational efficiency accounts of action hysteresis and theoretical accounts of syntactic priming both argue that reusing an existing plan is less costly than generating a novel plan. Despite these similarities across motor and language frameworks, research on planning in these domains has largely been conducted independently. The current study adapted an existing language paradigm to mirror the incremental nature of a manual motor task to investigate the presence of parallel hysteresis effects across domains. We observed asymmetries in production choice for both the motor and language tasks that resulted from the influence of prior history. Furthermore, these hysteresis effects were more exaggerated for subordinate production forms implicating an inverse preference effect that spanned domain. Consistent with computational efficiency accounts, across both task participants exhibited reaction time savings on trials in which they reused a recent production choice. Together, these findings lend support to the broader notion that there are common production biases that span both motor and language domains. [ABSTRACT FROM AUTHOR]

Upholding critical race theory (CRT)'s transdisciplinary approach to unveil the educational violence against Students of Color, we apply W.E.B. Du Bois's story of John from the chapter "Of the Coming of John" in The Souls of Black Folk. In doing so, we expose the problematic ideological and practical structures used for the identification and retention of Students of Color within gifted and talented programming. Examining the concepts of the Veil and double consciousness through the analogous relationship of Du Bois's John to a contemporary John, we parallel the insidious educational experiences of Students of Color that has remained relatively constant over time. [ABSTRACT FROM AUTHOR]

Graphic facilitation is a creative, robust visual communication process and tool that can be used by researchers for several benefits including improving data integrity; mitigating barriers between researchers and participants; promoting participants' ownership of data, decision-making, and creativity; and, co-creating knowledge, which is of particular interest among certain cultures and in some contexts. For Indigenous Peoples who traditionally use visual, oral, and narrative modalities as primary forms of communication, graphic facilitation is a methodology that aligns well with these modes of communicating. In this article, we describe our use of graphic facilitation in a community-led project exploring Indigenous parents' perceptions of community strengths, needs and priorities related to healthy early childhood development and optimal parenting. In collaboration with the Indigenous Friendship Centre in Hamilton, Canada, we held a Community Gathering that was facilitated by a graphic artist experienced in working with the Indigenous community; the findings resulting from the Gathering are presented. We discuss how researchers can use graphic facilitation as a tool to ensure adherence to the OCAP® principles of data ownership, control, access, and possession for the Indigenous community and describe the potential for mitigating power imbalances. Finally, considerations for researchers contemplating using graphic facilitation as a tool for research projects with Indigenous people and communities are presented. [ABSTRACT FROM AUTHOR]

Bias-based bullying (e.g., bullying related to race, weight, sexual orientation) is a common experience among youth, yet few school-based prevention programs explicitly address this type of bullying. This study explores whether schools that offer diversity education activities have lower rates of bias-based bullying among students compared to schools that do not offer these activities. Data came from two sources: the 2018 CDC School Profiles Survey (N = 216 schools) and the 2019 Minnesota Student Survey (N = 64,510 students). Multilevel logistic regression tested associations between diversity education activities (diversity clubs, lessons, or special events) and eight types of bias-based bullying among students, with attention to effect modification by relevant demographic characteristics. Students attending schools that offer a wider variety of diversity education opportunities had significantly lower odds of bullying about race, ethnicity, or national origin among boys of color (OR = 0.89, CI: 0.80, 1.00), about sexual orientation for gay, bisexual, and questioning boys (OR = 0.81, CI: 0.67, 0.97), and about disability for boys with a physical health problem (OR = 0.86, CI: 0.76, 0.99). Attending a school with more types of diversity education activities may protect vulnerable students against specific types of bias-based bullying and advance health equity. A diversity education is recommended as a key component of antibullying efforts and policy. [ABSTRACT FROM AUTHOR]

Objective: To determine whether initiating saline nasal irrigation after COVID-19 diagnosis reduces hospitalization and death in high-risk outpatients compared with observational controls, and if irrigant composition impacts severity. Methods: Participants 55 and older were enrolled within 24 hours of a + PCR COVID-19 test between September 24 and December 21, 2020. Among 826 screened, 79 participants were enrolled and randomly assigned to add 2.5 mL povidone-iodine 10% or 2.5 mL sodium bicarbonate to 240 mL of isotonic nasal irrigation twice daily for 14 days. The primary outcome was hospitalization or death from COVID-19 within 28 days of enrollment by daily self-report confirmed with phone calls and hospital records, compared to the CDC Surveillance Dataset covering the same time. Secondary outcomes compared symptom resolution by irrigant additive. Results: Seventy-nine high-risk participants were enrolled (mean [SD] age, 64 [8] years; 36 [46%] women; 71% Non-Hispanic White), with mean BMI 30.3. Analyzed by intention-to-treat, by day 28, COVID-19 symptoms resulted in one ED visit and no hospitalizations in 42 irrigating with alkalinization, one hospitalization of 37 in the povidone-iodine group, (1.27%) and no deaths. Of nearly three million CDC cases, 9.47% were known to be hospitalized, with an additional 1.5% mortality in those without hospitalization data. Age, sex, and percentage with pre-existing conditions did not significantly differ by exact binomial test from the CDC dataset, while reported race and hospitalization rate did. The total risk of hospitalization or death (11%) was 8.57 times that of enrolled nasal irrigation participants (SE = 2.74; P =.006). Sixty-two participants completed daily surveys (78%), averaging 1.8 irrigations/day. Eleven reported irrigation-related complaints and four discontinued use. Symptom resolution was more likely for those reporting twice daily irrigation (X 2 = 8.728, P =.0031) regardless of additive. Conclusion: SARS-CoV-2+ participants initiating nasal irrigation were over 8 times less likely to be hospitalized than the national rate. [ABSTRACT FROM AUTHOR]

Captopril Alleviates Epilepsy and Cognitive Impairment by Attenuation of C3-Mediated Inflammation and Synaptic Phagocytosis Dong X, Fan J, Lin D, Wang X, Kuang H, Gong L, Chen C, Jiang J, Xia N, He D, Shen W. J Neuroinflammation. 2022;19(1):226. doi:10.1186/s12974-022-02587-8 Evidence from experimental and clinical studies implicates immuno-inflammatory responses as playing an important role in epilepsy-induced brain injury. Captopril, an angiotensin-converting enzyme inhibitor (ACEi), has previously been shown to suppress immuno-inflammatory responses in a variety of neurological diseases. However, the therapeutic potential of captopril on epilepsy remains unclear. In the present study, Sprague Dawley (SD) rats were intraperitoneally subjected to kainic acid (KA) to establish a status epilepticus. Captopril (50 mg/kg, i.p.) was administered daily following the KA administration from day 3 to 49. We found that captopril efficiently suppressed the KA-induced epilepsy, as measured by electroencephalography. Moreover, captopril ameliorated the epilepsy-induced cognitive deficits, with improved performance in the Morris water maze, Y-maze and novel objective test. RNA sequencing (RNA-seq) analysis indicated that captopril reversed a wide range of epilepsy-related biological processes, particularly the glial activation, complement system-mediated phagocytosis and the production of inflammatory factors. Interestingly, captopril suppressed the epilepsy-induced activation and abnormal contact between astrocytes and microglia. Immunohistochemical experiments demonstrated that captopril attenuated microglia-dependent synaptic remodeling presumably through C3–C3ar-mediated phagocytosis in the hippocampus. Finally, the above effects of captopril were partially blocked by an intranasal application of recombinant C3a (1.3 μg/kg/day). Our findings demonstrated that captopril reduced the occurrence of epilepsy and cognitive impairment by attenuation of inflammation and C3-mediated synaptic phagocytosis. This approach can easily be adapted to long-term efficacy and safety in clinical practice. [ABSTRACT FROM AUTHOR]

Seizure-Induced Strengthening of a Recurrent Excitatory Circuit in the Dentate Gyrus Is Proconvulsant Nasrallah K, Frechou MA, Yoon YJ, Persaud S, Gonçalves JT, Castillo PE. Proc Natl Acad Sci. 2022;119(32):e2201151119. doi:10.1073/pnas.2201151119 Epilepsy is a devastating brain disorder for which effective treatments are very limited. There is growing interest in early intervention, which requires a better mechanistic understanding of the early stages of this disorder. While diverse brain insults can lead to epileptic activity, a common cellular mechanism relies on uncontrolled recurrent excitatory activity. In the dentate gyrus, excitatory mossy cells (MCs) project extensively onto granule cells (GCs) throughout the hippocampus, thus establishing a recurrent MC-GC-MC excitatory loop. MCs are implicated in temporal lobe epilepsy, a common form of epilepsy, but their role during initial seizures (i.e., before the characteristic MC loss that occurs in late stages) is unclear. Here, we show that initial seizures acutely induced with an intraperitoneal kainic acid (KA) injection in adult mice, a well-established model that leads to experimental epilepsy, not only increased MC and GC activity in vivo but also triggered a brain-derived neurotrophic factor (BDNF)-dependent long-term potentiation (LTP) at MC-GC excitatory synapses. Moreover, in vivo induction of MC-GC LTP using MC-selective optogenetic stimulation worsened KA-induced seizures. Conversely, Bdnf genetic removal from GCs, which abolishes LTP, and selective MC silencing were both anticonvulsant. Thus, initial seizures are associated with MC-GC synaptic strengthening, which may promote later epileptic activity. Our findings reveal a potential mechanism of epileptogenesis that may help in developing therapeutic strategies for early intervention. [ABSTRACT FROM AUTHOR]

Sexual violence perpetration (SVP), including coerced, physically forced, and alcohol- or drug-facilitated unwanted sex, occurs frequently in adolescence and may represent a risk factor for future perpetration. Sexual violence victimization (SVV) has been found to be a risk factor for increased rates of depression and posttraumatic stress disorder (PTSD); however, the associations of SVP with depression or posttraumatic stress symptoms (PTSS) have been less well described. This study examined associations between symptoms of depression and PTSS with SVP in the prior 12 months among high school students. In this cross-sectional analysis, a representative sample of public high school students (ninth–12th grades) completed self-reported surveys on peer SVP and SVV within the past year. Among 16,784 students completing surveys, 7.2% disclosed SVP against another high school student in the past 12 months; 64.4% of students disclosing SVP also experienced SVV. Both SVP and SVV, alone or in combination, were associated with a greater likelihood of symptoms of depression or PTSS. These associations were similar by sex and sexual minority status (e.g., lesbian, gay, bisexual, transgender, and queer [LGBTQ+]). These findings highlight the need for continued primary prevention efforts. Additional screening to recognize adolescent SVP can allow both early treatment of depression and PTSD and address the individual risks of SVP to reduce subsequent repeated sexual assaults. [ABSTRACT FROM AUTHOR]

Canada and the United States have recently evaluated the decriminalization of drugs as multiple provinces and states put motions forward to consider drug decriminalization legislation. The influence of factors such as demographics, substance use, perceived substance use risk, and personality have not been widely studied in predicting attitudes toward drug decriminalization. A total of 504 participants were drawn from university (n = 269, 53.37%) and community samples (n = 235, 46.63%) through online social media groups and posts (i.e., Facebook, Twitter, Reddit, etc). Analyses indicated that male gender, single or non-married relationship status, living outside of Atlantic Canada, higher problematic alcohol use scores, lower Extraversion, higher Open-mindedness, and lower perceived risk of using substances emerged as significant predictors of support for drug decriminalization. These findings have important implications as public attitudes toward a substance influence drug policy. [ABSTRACT FROM AUTHOR]

During the COVID-19 pandemic, many lesbian, gay, bisexual, transgender, and queer (LGBTQ) youth lived with family members in forced togetherness. Using the Texas Queer Youth COVID Study, a longitudinal qualitative study of 26 LGBTQ youth in Texas, we argue that the social conditions of the COVID-19 pandemic impacted relationships between LGBTQ youth and their family in dynamic ways, including cultivating intimacy and magnifying new or existing conflicts. LGBTQ youth could not use their preferred strategies of avoidance to handle relatives’ conservative pandemic politics and conspiracy theories. For Latinx LGBTQ youth, the intensified familism required during the shelter-in-place of the pandemic cultivated more intimacy but also became a justification for distance for youth trying to avoid homophobic relatives. These findings contribute to the study of LGBTQ youth and family relations, along with the impact of social isolation on family life. [ABSTRACT FROM AUTHOR]

We examined the high school experiences of 133 African American, Latina/o, and biracial college students through employing a mixed methods concurrent nested design, including survey analysis and qualitative content analysis, to identify themes and the extent school counselors provided assistance with promoting academic and college readiness. The American School Counselor Association (ASCA) National Model and Multicultural and Social Justice Counseling Competencies (MSJCC) were used as a conceptual framework to guide the study. Findings included the need for greater support from school counselors, to strengthen collaborative efforts across school community members and access to academic resources. Implications for counseling practice in urban schools are provided. [ABSTRACT FROM AUTHOR]

Couples coping with depression are prone to unique and pervasive sexual intimacy challenges and experience troubles communicating effectively. Successful sexual communication improves sexual and relationship satisfaction, making communication particularly important for couples facing sexual difficulties. In this study, the relational turbulence model serves as a framework to examine associations between sexual communication and both sexual and relationship satisfaction in couples (N = 106) in which one or both partners live with depression. Results of a cross-sectional survey suggest sexual communication mediates relationships between both relational uncertainty and interference from a partner with sexual/relationship satisfaction. Results illuminate functions of communication about sex as couples navigate sexual and relational effects of depression. [ABSTRACT FROM AUTHOR]

Food pantries are responsible for the direct distribution of food to low-income households. While food pantries may be concerned about the nutritional quality of the food they are serving, they may have limited resources to adopt and implement nutrition policies to support efforts to promote high nutritional quality of the food served. Guided by the RE-AIM (Reach, Effectiveness or Efficacy, Adoption, Implementation, Maintenance) framework, this qualitative study explored the degree of implementation of nutrition policies at food pantries, as well as the barriers to implementation in those pantries that had not adopted a nutrition policy. Semistructured interviews were conducted with 10 food pantry directors: seven pantries with a formal nutrition policy and three with an informal nutrition policy. Using a thematic analysis method, results demonstrated themes from the interviews with policy-adopting pantries to be barriers, enforcement, delivery of the policy, unexpected consequences, and fidelity to the policy. A targeted intervention that builds on this research and focuses on building the capacity of food pantries to develop, adopt, and implement nutrition policies as well as helping to increase fidelity to the policy would be beneficial to continue to improve the food donated and distributed at food pantries. By supporting food pantries in the development, adoption, and implementation of nutrition policies, researchers can play an important role in improving the quality of food in the emergency food network. [ABSTRACT FROM AUTHOR]

Many older adults do not engage in age-based prevention despite evidence to support reduced health risks and enhanced successful aging. The purpose of this study was to determine whether self-perceived aging (SPA) differed among older adults by age (young-old vs. old-old), participation in healthy lifestyle behaviors, screening, vaccinations, and self-rated health. Community-dwelling older adults (n=204) completed questionnaires reporting their SPA, self-rated health, and participation in recommended preventive healthy lifestyle behaviors, screening, and vaccinations. Our findings indicated that adults who were older and engaged in more preventive health behaviors, yet had lower self-rated health, tended to have better SPA. Prevention was greater in older adults who scored higher on aging well and aging successfully. Old-old (75 years or older) participants scored higher on aging successfully than those who were younger. Self-rated health was inversely related to SPA scores. Reporting poor or fair health did not diminish positive SPA in this sample. [ABSTRACT FROM AUTHOR]

Participants were 5,831 women in their third trimester of pregnancy, part of a large, longitudinal, pre-birth national cohort study. Women reported on their experience of pushing and shoving, throwing or breaking objects within their relationship over the past month. Univariable regression models examined the association of a large number of potential risk and protective factors. Those significant at the univariable level were carried forward into final multivariable analyses, stratified by New Zealand's four main ethnic groups: European, Māori, Pacific, and Asian peoples. Relationship commitment, reduced family cohesion, and perceived stress were associated with increased risk across ethnic groups. [ABSTRACT FROM AUTHOR]

A number of researchers have examined undocumented migration from Central America. This literature lacks information about adult beliefs regarding the motivations of minors who journey from Central America unaccompanied and undocumented. Using data from a recent survey conducted in Honduras, we examine adult Hondurans' beliefs about why unaccompanied minors leave the country unaccompanied. The dependent variable is a dummy variable that measures "why children leave the country." Predictor variables are attitudes toward smuggling, willingness to leave without documentation, deportation experience, age, income, and residence in the northern part of Honduras. Using multinomial logistic regression, we find support for four of the eight hypotheses. Findings indicate that adults from the northern region are most likely to believe minors would leave for reasons associated with undocumented immigration. Those who are younger, with lower incomes, and with less access to sanitation are more likely to believe minors would leave without documentation. [ABSTRACT FROM AUTHOR]

Although the effects of focus of attention (FOA) on the performance of gross motor skills are now well understood, less is known about the role of FOA in naturalistic classroom settings where learners are engaged in the ongoing acquisition and refinement of complex motor skills. The purpose of this study, the first of its kind, was to explore how music teachers focus learners' attention on physical actions (internal focus) and on the effects of those actions (external focus). We recorded three experienced band directors teaching beginner classes (sixth graders) and completed a content analysis of video recordings to describe (a) teachers' use of internally focused (IF) and externally focused (EF) verbalizations and (b) patterns among IF and EF verbalizations. These teachers most often directed student attention to internal aspects of performance, and they also paired IF and EF statements to clearly convey how those actions affect external outcomes. Although our descriptive multiple case study design precludes generalization, these data suggest possibilities for future studies that could explore relationships between teachers' FOA verbalizations and skill development in music classrooms. [ABSTRACT FROM AUTHOR]

In addition to the emotion work that occurs in families, I theorize that adult children also engage in comfort work with their parents, navigations, and strategies to reduce the discomfort of parents with their adult children's lives. This study analyzes how gay and lesbian adults navigate the participation of their parents at events run by lesbian, gay, bisexual, transgender, and queer (LGBTQ) clubs or "krewes" in the Gulf South during Carnival season. I conducted over 50 days of ethnographic fieldwork during Mardi Gras events and 26 interviews with gay and lesbian members of krewes in Mobile, Alabama, and Baton Rouge, Louisiana. I argue that gay and lesbian adults negotiate their parents' attendance in gay spaces by comfort work, mostly by creating comfort for their parents and managing disreputability through education. Ultimately, I conclude that parents' attendance at LGBTQ events results in supportive reciprocation, "payoffs" of acceptance that are rich in emotional and symbolic significance for adult children. [ABSTRACT FROM AUTHOR]

Mental health outcomes have shown dramatic changes over the past half-century, yet these trends are still underexplored. I utilize an age-period-cohort analysis of the National Health Interview Survey from 1997 to 2017 (N = 627,058) to disentangle trends in mental health outcomes in the United States over time. Specifically, I leverage the contrast between reported psychological distress and rates of mental health treatment to isolate which has changed, how, and for whom. There is little evidence that psychological distress is worsening over time. Yet, treatment seeking has increased over the past 20 years. The increase in treatment seeking is best modeled as a period effect, providing initial evidence that the historical context has influenced responses to mental health over time for Americans of all ages and birth cohorts. I conclude with potential mechanisms and implications for future mental health research. [ABSTRACT FROM AUTHOR]

Visual working memory for features and bindings is susceptible to age-related decline. Two experiments were used to examine whether older adults are able to strategically prioritise more valuable information in working memory and whether this could reduce age-related impairments. Younger (18–33 years) and older (60–90 years) adults were presented with coloured shapes and, following a brief delay, asked to recall the feature that had accompanied the probe item. In Experiment 1, participants were either asked to prioritise a more valuable object in the array (serial position 1, 2, or 3) or to treat them all equally. Older adults exhibited worse overall memory performance but were as able as younger adults to prioritise objects. In both groups, this ability was particularly apparent at the middle serial position. Experiment 2 then explored whether younger and older adults' prioritisation is affected by presentation time. Replicating Experiment 1, older adults were able to prioritise the more valuable object in working memory, showing equivalent benefits and costs as younger adults. However, processing speed, as indexed by presentation time, was shown not to limit strategic prioritisation in either age group. Taken together, these findings demonstrate that, although older adults have poorer visual working memory overall, the ability to strategically direct attention to more valuable items in working memory is preserved across ageing. [ABSTRACT FROM AUTHOR]

Differences in monolayer and three-dimensional (3D) culture systems have been recognized for several years. Despite the recognized importance of 3D systems, low cost and convenience of monolayer culture are still readily used for metabolic and nutritional studies. Here, we present part 1 of a 2-part series that will highlight (1) a novel and cost-effective model for culturing 3T3-L1 preadipocytes in 3D agarose as well as (2) an initial study showing the successful use of this 3D model for experimental analysis of these cells treated with cinnamon extract while suspended in agarose. In part 1, we provide a full characterization of the model system for the 3T3-L1 cells that demonstrate the functionality and convenience of this system. Importantly, we note spontaneous differentiation to adipocytes while cultured under these methods, independent of chemical induction. We present a 2.5-week time course with rounded cells forming vacuoles as early as 24 hours and accumulation of lipid detectable by Oil Red O stain at 0.5 weeks. Serum selection, lipid volume determination, and cell size are characterized. We conclusively demonstrate adipogenesis based on a peroxisome proliferator-activated receptor y (PPARY) detection using immunohistochemistry (IHC) of sections from these 3D cultures. Methods, materials and recommendations are described as well as proposed benefits to the use of this culture system for 3T3-L1 cells. [ABSTRACT FROM AUTHOR]

Anxiety is an under-investigated comorbidity in heart disease patients. Optimism/hope is a character strength that indicates confidence or favorable expectation about the future. Previous research has consistently reported optimal health outcomes among optimists. However, many studies have lacked adjustment for medical confounders and/or used small clinical samples. To bridge this gap, we tested the hypothesis that optimism/hope was inversely related to anxiety in 400+ patients with advanced heart disease during the stressful waiting period prior to open-heart surgery. The findings supported the hypothesis after controlling for general health, illness impact, behavioral risks, and cardiac medical indices used by surgeons. [ABSTRACT FROM AUTHOR]

American Academy of Pediatrics (AAP) guidelines for children with Down syndrome (DS) include assessment for celiac disease (CD), although data to support this recommendation have been inconsistent. We determined the incidence of CD among children with DS in a population-based birth cohort of children born from 1976 to 2000 in Olmsted County, Minnesota. Individuals with karyotype-confirmed DS and CD (using diagnosis codes, positive serology, and duodenal biopsies) were identified. The incidence of CD in DS was compared with the published incidence of CD for Olmsted County residents (17.4 [95% confidence interval = 15.2-19.6] per 100 000 person-years). Among 45 individuals with DS from the birth cohort, 3 (6.7%) were identified with positive celiac serology and confirmatory biopsies at ages 9, 12, and 23 years, for an incidence of 325 per 100 000 person-years. Thus, individuals with DS have more than 18 times the incidence rate of CD compared with the general population, supporting the AAP guidelines. [ABSTRACT FROM AUTHOR]

However, the potential of distinct lymphocyte subsets alone to elicit key clinicopathological sequelae of LE potentially inducing temporal lobe epilepsy (TLE) with chronic spontaneous seizures and hippocampal sclerosis (HS) is unresolved. Commentary Temporal lobe epilepsy (TLE) is a common type of epilepsy characterized by focal seizures that can be drug-resistant.[1] In TLE, the hippocampal and amygdala brain regions are typically damaged and, as a result, cognitive deficits often develop along with recurrent unprovoked seizures.[1],[2] Severe neuronal loss, gliosis, and inflammation in the hippocampus are some of the neuropathological hallmarks of TLE. [Extracted from the article]

The Home Observation for Measurement of the Environment (HOME) Inventory is designed to assess the quality and quantity of support, stimulation, and structure provided to children in the home environment. HOME has been widely used for research and applied purposes. We focused on an abbreviated version of the Early Adolescent HOME (EA-HOME-A) that was administered to 15-year-old adolescents and their parents (N = 958) as part of the NICHD (National Institute of Child Health and Human Development) Study of Early Child Care and Youth Development. Our study had two objectives. First, we hypothesized and tested a bifactor model that specified a general factor in support of the use of the HOME total score and group factors for subsets of items in support of the content domain scores. Second, we applied structural equation modeling to relate the EA-HOME-A factors to outcome factors assessing maladaptive behaviors, autonomy, self-control, and cognitive–academic performance. The results supported the construct validity of the EA-HOME-A with respect to its internal structure as well as its correlates. [ABSTRACT FROM AUTHOR]

Researchers have been identifying microplastics in environmental samples dating back to the 1970s. Today, microplastics are a recognized environmental pollutant attracting a large amount of public and government attention, and in the last few years the number of scientific publications has grown exponentially. An underlying theme within this research field is to achieve a consensus for adopting a set of appropriate procedures to accurately identify and quantify microplastics within diverse matrices. These methods should then be harmonized to produce quantifiable data that is reproducible and comparable around the world. In addition, clear and concise guidelines for standard analytical protocols should be made available to researchers. In keeping with the theme of this special issue, the goals of this focal point review are to provide researchers with an overview of approaches to isolate and extract microplastics from different matrices, highlight associated methodological constraints and the necessary steps for conducting procedural controls and quality assurance. Simple samples, including water and sediments with low organic content, can be filtered and sieved. Stepwise procedures require density separation or digestion before filtration. Finally, complex matrices require more extensive steps with both digestion and density adjustments to assist plastic isolation. Implementing appropriate methods with a harmonized approach from sample collection to data analysis will allow comparisons across the research community. [ABSTRACT FROM AUTHOR]

Microplastics are a diverse category of pollutants, comprising a range of constituent polymers modified by varying quantities of additives and sorbed pollutants, and exhibiting a range of morphologies, sizes, and visual properties. This diversity, as well as their microscopic size range, presents numerous barriers to identification and enumeration. These issues are addressed with the application of physical and chemical analytical procedures; however, these present new problems associated with researcher training, facility availability and cost, especially for large-scale monitoring programs. Perhaps more importantly, the classifications and nomenclature used by individual researchers to describe microplastics remains inconsistent. In addition to reducing comparability between studies, this limits the conclusions that may be drawn regarding plastic sources and potential environmental impacts. Additionally, where particle morphology data is presented, it is often separate from information on polymer distribution. In establishing a more rigorous and standardized visual identification procedure, it is possible to improve the targeting of complex analytical techniques and improve the standards by which we monitor and record microplastic contamination. Here we present a simple and effective protocol to enable consistent visual processing of samples with an aim to contribute to a higher degree of standardization within the microplastic scientific community. This protocol will not eliminate the need for non-subjective methods to verify plastic objects, but it will standardize the criteria by which suspected plastic items are identified and reduce the costs associated with further analysis. [ABSTRACT FROM AUTHOR]

Although jurisdictions have attempted to improve their response procedures, sexual assault cases are often insufficiently investigated. This study examines the survey responses of 460 female sexual assault survivors regarding their experiences with response personnel. Overall satisfaction with response services was 66.1%. Victim advocates and forensic nurses received higher satisfaction ratings than did patrol officers, detectives, and State's Attorney's Office staff. The present study also found that 65% of the variance in overall satisfaction was accounted for by four personnel behaviors: respectful treatment, clearly explained procedures, believed their stories, and demonstrated cultural sensitivity. Policy implications and suggestions for future research are discussed. [ABSTRACT FROM AUTHOR]

Introduction: Guiding Eyes for the Blind (GEB) breeds, raises, and trains dogs to serve as guides for people with visual impairments (i.e., those who are blind or have low vision). Their puppy raising program enlists volunteers to foster puppies for a year or more, providing socialization, basic skills training, and comfort, so they will grow into confident dogs. The volunteers must ultimately return these dogs to Yorktown Heights, New York, to begin training. For raisers, the emotional toll of this parting can be great. Methods: Utilizing the 2017 GEB Puppy Raiser Survey data, this study analyzed factors that might affect emotional grief and the decision to continue to raise puppies. Cross-tabulation and ordinal regression analyses examined the associations of puppy raiser demographics (i.e., gender, age, marital status, and employment), organizational integration (i.e., number of dogs raised for GEB, perceptions on how well GEB supports raisers in returning a dog, belief that they are part of the GEB community, and formation of friendships), and goal attainment (perceptions on how well GEB communicated progress in training, whether they raised a dog accepted for guide training, and whether they attended a graduation ceremony) with self-reported emotional difficulty, recovery time, and likelihood of raising another puppy. Results: Although the demographics of the raiser had minimal effect on emotional difficulty, recovery time, and likelihood of raising again, factors that measured organization integration and goal attainment were associated with grief and continued participation. Discussion: Raiser demographics are not strong predictors of grief and continued raising; however, a welcoming organization that communicates effectively will inspire volunteers' commitment. Implications: With a growing need for assistance dogs and puppy raisers, identifying and addressing the challenges that these volunteers face is critical if skilled and dedicated raisers are to be recruited and retained. [ABSTRACT FROM AUTHOR]

How do physical digital inequalities persist as technology becomes commonplace? We consider this question using surveys and focus groups with U.S. college students, a group that has better than average connectivity. Findings from a 748-person nonrepresentative survey revealed that ownership and use of cellphones and laptops were nearly universal. However, roughly 20% of respondents had difficulty maintaining access to technology (e.g., broken hardware, data limits, connectivity problems, etc.). Students of lower socioeconomic status and students of color disproportionately experienced hardships, and reliance on poorly functioning laptops was associated with lower grade point averages. Focus group and open-ended data elaborate these findings. Findings quantitatively validate the technology maintenance construct, which proposes that as access to information and communication technology peaks, the digital divide is increasingly characterized by the (in)ability to maintain access. Data highlight overlooked nuances in digital access that may inform social disparities and the policies that may mitigate them. [ABSTRACT FROM AUTHOR]

Retrospective studies using adult self-report data have demonstrated that adverse childhood experiences (ACEs) increase risk of violence perpetration and victimization. However, research examining the associations between adolescent reports of ACE and school violence involvement is sparse. The present study examines the relationship between adolescent reported ACE and multiple types of on-campus violence (bringing a weapon to campus, being threatened with a weapon, bullying, fighting, vandalism) for boys and girls as well as the risk of membership in victim, perpetrator, and victim–perpetrator groups. The analytic sample was comprised of ninth graders who participated in the 2013 Minnesota Student Survey (n ~ 37,000). Multinomial logistic regression models calculated the risk of membership for victim only, perpetrator only, and victim–perpetrator subgroups, relative to no violence involvement, for students with ACE as compared with those with no ACE. Separate logistic regression models assessed the association between cumulative ACE and school-based violence, adjusting for age, ethnicity, family structure, poverty status, internalizing symptoms, and school district size. Nearly 30% of students were exposed to at least one ACE. Students with ACE represent 19% of no violence, 38% of victim only, 40% of perpetrator only, and 63% of victim–perpetrator groups. There was a strong, graded relationship between ACE and the probability of school-based victimization: physical bullying for boys but not girls, being threatened with a weapon, and theft or property destruction (p s <.001) and perpetration: bullying and bringing a weapon to campus (p s <.001), with boys especially vulnerable to the negative effects of cumulative ACE. We recommend that schools systematically screen for ACE, particularly among younger adolescents involved in victimization and perpetration, and develop the infrastructure to increase access to trauma-informed intervention services. Future research priorities and implications are discussed. [ABSTRACT FROM AUTHOR]

Humans and animals share a unique bond. Professionals are capitalizing on the human–animal bond by incorporating animals into therapy, forensic interviews, and the courtroom. However, the mnemonic consequence for including dogs in forensic interviews has not been empirically evaluated. In the current study, we examined whether the use of dogs increases the quantity of verbal reports for emotional events. Undergraduate participants were randomly assigned to dog or no dog conditions. Participants were interviewed about positive experienced events and negative experienced events. All interviews were audio recorded, transcribed verbatim, and analyzed for quantity of new information. Participants shared more new information in negative event reports with a dog present than with no dog present. There were no significant differences in the quantity of information disclosed for positive event memories between dog conditions. Canine-assisted interviews may provide comfort to people, resulting in more elaborative autobiographical reports about negative stressful events. [ABSTRACT FROM AUTHOR]

Young peoples' acceptance and use of nontraditional, descriptive identity labels (e.g., pansexual, genderqueer) require nurses to consider moving beyond use of traditional terms (e.g., gay, transgender). This mixed methods study explores (a) labels used by sexual orientation and gender identity (SOGI) minority youth, (b) their expressed importance and meaning of these labels, and (c) differences in label usage. Sixty-six SOGI minority adolescents in British Columbia, Minnesota, and Massachusetts (mean age = 16.6) participated in "go-along" interviews; during interviews, 42 (63.6%) commented on labels. Chi-square and t tests were used to compare traditional versus nontraditional labels across participant demographic categories. Inductive thematic analysis was used to identify representative themes. Approximately, 1/3 of participants used nontraditional sexual orientation identity labels; this finding was associated with a trans identity and nontraditional gender labels. Using terminology that is meaningful and representative to the youth themselves has potential to facilitate representative research and welcoming environments in practice. [ABSTRACT FROM AUTHOR]

While the college years present an ideal time for chronic disease prevention, students often do not seek services or perceive themselves to be at health risk. Researchers at Bowling Green State University have been exploring the health patterns of first-year university students for several years, initially as a health-based research study (August 2012 to May 2016) and currently through a first-year seminar course for students transitioning from high school to college (August 2016 to present). This article describes the evolution of data sharing techniques from passively presented paper feedback sheets (focusing on individual health assessments) to highly interactive classroom discussions stemming from viewings of animated case study videos (designed based on whole group themes). Qualitative feedback from students and faculty has been positive, suggesting that animated case study videos are an effective strategy for engaging students in critical thinking about physical and mental health concerns affecting their peer group. Practical recommendations for using animated case studies in diverse educational settings are provided. [ABSTRACT FROM AUTHOR]

Microglia are the resident immune cells and professional phagocytes of the central nervous system. However, little is known about the contribution of their phagocytic signaling to the neuropathology and pathophysiology of epilepsy. Here, we summarize and discuss the implications of recent evidence supporting that aberrant microglia phagocytic activity and alterations in phagocytosis signaling molecules occur in association with microglia–neuronal contacts, neuronal/synaptic loss, and spontaneous recurrent seizures in human and preclinical models of epilepsy. This body of evidence provides strong support that the microglial contribution to epileptogenic networks goes beyond inflammation, and suggests that phagocytic signaling molecules may be novel therapeutic targets for epilepsy. [ABSTRACT FROM AUTHOR]

Objectives: Publicity on incidents of electronic nicotine delivery systems (ENDS) exploding or catching fire may influence smokers' risk perceptions and decisions about using ENDS for quitting smoking. We examined combustible cigarette smokers' perceptions of the possibility of injury from exploding ENDS and the relationship of those perceptions to ENDS use, perceived risk relative to smoking, and perceived costs and benefits of ENDS use. Methods: We used data from the 2017 Tobacco Products and Risk Perceptions Survey of a national probability sample of US adults, conducted online in August and September 2017. The analytic sample consisted of 1181 current combustible cigarette smokers aware of ENDS. Results: Among combustible cigarette smokers aware of ENDS, a medium/high perceived risk of injury from exploding ENDS was associated with lower odds of current ENDS use (adjusted odds ratio [aOR] = 0.50; 95% confidence interval [CI], 0.30-0.86; P =.01), perceiving ENDS to be at least as harmful as cigarettes (aOR = 3.22; 95% CI, 2.11-4.93; P <.001), and feeling that the negatives of ENDS use outweighed the positives (aOR = 3.50; 95% CI, 2.00-6.14; P <.001). Conclusions: Product standards, improved product labeling, and education about how to properly use, charge, and store ENDS can help protect consumers from injury. Communication efforts should seek to fully inform combustible cigarette smokers about both the absolute health risks of ENDS, including risk of explosions, and their relative risks compared with those of cigarette smoking. [ABSTRACT FROM AUTHOR]

The internationalization of higher education results in 4.6 million students attending colleges and universities outside their home countries. In the United States and other countries, there is significant underrepresentation of women among inbound international higher education students. Gender equality in education cannot be achieved so long as women are underrepresented in participation in this important educational venue. To better understand the drivers of gender inequalities in international higher education, this study examines the low participation rate by women coming to the United States by comparing it with participation data for women coming to the United Kingdom and Germany. Gender participation rates from both source regions and countries vary by destination country. By exploring the geography of gender inequality in international higher education, decision makers can better understand barriers to achieving international gender equality goals. [ABSTRACT FROM AUTHOR]

Studies assessing differences between intraracial and interracial marriages typically use race data from one time point. Yet because racial identification can vary across time, context, or perspective, whether a relationship is defined as intraracial or interracial can also differ. We use a sample of 2,845 respondents from the National Longitudinal Survey of Youth, 1997, whose marriages are intraracial (based on 2002 data) to examine whether marital stability differs for those whose racial identification varied across waves and whether this effect is moderated by gender. Approximately 6% of respondents in intraracial relationships had inconsistent racial identities. We also find evidence that the association between racial variation and marital stability differs by gender. Women whose race varied are more likely to divorce than any other group, including static-race couples and men whose race varied. More attention should be given to intraracial heterogeneity, especially as groups that are more likely to have varying racial identities grow in number. [ABSTRACT FROM AUTHOR]

Dendritic Pathology, Spine Loss and Synaptic Reorganization in Human Cortex From Epilepsy Patients Rossini L, De Santis D, Mauceri RR, Tesoriero C, Bentivoglio M, Maderna E, Maiorana A, Deleo F, de Curtis M, Tringali G, Cossu M, Tumminelli G, Bramerio M, Spreafico R, Tassi L, Garbelli R. Brain. 2021;144(1):251-265. doi:10.1093/brain/awaa387 Neuronal dendritic arborizations and dendritic spines are crucial for a normal synaptic transmission and may be critically involved in the pathophysiology of epilepsy. Alterations in dendritic morphology and spine loss mainly in hippocampal neurons have been reported both in epilepsy animal models and in human brain tissues from patients with epilepsy. However, it is still unclear whether these dendritic abnormalities relate to the cause of epilepsy or are generated by seizure recurrence. We investigated fine neuronal structures at the level of dendritic and spine organization using Golgi impregnation, and analyzed synaptic networks with immunohistochemical markers of glutamatergic (vGLUT1) and GABAergic (vGAT) axon terminals in human cerebral cortices derived from epilepsy surgery. Specimens were obtained from 28 patients with different neuropathologically defined etiologies: type Ia and type II focal cortical dysplasia, cryptogenic (no lesion) and temporal lobe epilepsy with hippocampal sclerosis. Autoptic tissues were used for comparison. Three-dimensional reconstructions of Golgi-impregnated neurons revealed severe dendritic reshaping and spine alteration in the core of the type II focal cortical dysplasia. Dysmorphic neurons showed increased dendritic complexity, reduction of dendritic spines, and occasional filopodia-like protrusions emerging from the soma. Surprisingly, the intermingled normal-looking pyramidal neurons also showed severe spine loss and simplified dendritic arborization. No changes were observed outside the dysplasia (perilesional tissue) or in neocortical postsurgical tissue obtained in the other patient groups. Immunoreactivities of vGLUT1 and vGAT showed synaptic reorganization in the core of type II dysplasia characterized by the presence of abnormal perisomatic baskets around dysmorphic neurons, in particular those with filopodia-like protrusions, and changes in vGLUT1/vGAT expression. Ultrastructural data in type II dysplasia highlighted the presence of altered neuropil engulfed by glial processes. Our data indicate that the fine morphological aspect of neurons and dendritic spines are normal in epileptogenic neocortex, with the exception of type II dysplastic lesions. The findings suggest that the mechanisms leading to this severe form of cortical malformation interfere with the normal dendritic arborization and synaptic network organization. The data argue against the concept that long-lasting epilepsy and seizure recurrence per se unavoidably produce a dendritic pathology. [ABSTRACT FROM AUTHOR]

Vascular Endothelial Growth Factor Receptor-3 Regulates Astroglial Glutamate Transporter-1 Expression via mTOR Activation in Reactive Astrocytes Following Pilocarpine-Induced Status Epilepticus Jeong KH, Cho KO, Lee MY, Kim SY, Kim WJ. Glia. 2021;69(2):296-309. doi:10.1002/glia.23897. PMID: 32835451. Recent evidence has shown that the vascular endothelial growth factor (VEGF) system plays a crucial role in several neuropathological processes. We previously reported an upregulation of VEGF-C and its receptor, VEGFR-3, in reactive astrocytes after the onset of status epilepticus (SE). However, it remains unknown, which molecules act as downstream signals following VEGFR-3 upregulation and are involved in astrogliosis after SE. Therefore, we investigated whether VEGFR-3 upregulation within reactive astrocytes is associated with the activation of mammalian target of rapamycin (mTOR) signaling, which we confirmed by assaying for the phosphorylated form of S6 protein (pS6), and whether VEGFR-3-mediated mTOR activation induces astroglial glutamate transporter-1 (GLT-1) expression in the hippocampus after pilocarpine-induced SE. We found that spatiotemporal expression of pS6 was consistent with VEGFR-3 expression in the hippocampus after SE and that both pS6 and VEGFR-3 were highly expressed in SE-induced reactive astrocytes. Treatment with the mTOR inhibitor rapamycin decreased astroglial VEGFR-3 expression and GLT-1 expression after SE. Treatment with a selective inhibitor for VEGFR-3 attenuated astroglial pS6 expression as well as suppressed GLT-1 expression and astroglial reactivity in the hippocampus after SE. These findings demonstrate that VEGFR-3-mediated mTOR activation could contribute to the regulation of GLT-1 expression in reactive astrocytes during the subacute phase of epilepsy. In conclusion, the present study suggests that VEGFR-3 upregulation in reactive astrocytes may play a role in preventing hyperexcitability induced by continued seizure activity. [ABSTRACT FROM AUTHOR]

Acute respiratory distress syndrome (ARDS) remains a common complication associated with significant negative outcomes in critically ill patients. Lung-protective mechanical ventilation strategies remain the cornerstone in the management of ARDS. Several therapeutic options are currently available including fluid management, neuromuscular blocking agents, prone positioning, extracorporeal membrane oxygenation, corticosteroids, and inhaled pulmonary vasodilating agents (prostacyclins and nitric oxide). Unfortunately, an evidence-based, standard-of-care approach in managing ARDS beyond lung-protective ventilation remains elusive, contributing to significant variability in clinical practice. Although the optimal therapeutic strategy for managing moderate to severe ARDS remains extremely controversial, therapies supported with more robust clinical evidence should be considered first. The purpose of this narrative review is to discuss the published clinical evidence for both pharmacologic and nonpharmacologic management strategies in adult patients with moderate to severe ARDS as well as to discuss practical considerations for implementation. [ABSTRACT FROM AUTHOR]

This 2-year study investigated the accommodations and support services preferred by college students with autism spectrum disorder using sequential mixed methods non-experimental survey and semi-structured follow-up interviews. Students with autism spectrum disorder reported using both academic and non-academic supports with frequency (e.g. extended time on exams, transition program), using academic supports in line with other disability populations, and using non-academic supports connecting them one-to-one with a faculty member or coach as preferred (e.g. academic coach, counselor, faculty mentor). Findings suggest a need for university disability service centers, counseling services, and faculty to work together to develop systematic support systems for college students with autism spectrum disorder. [ABSTRACT FROM AUTHOR]

Research has shown that judgments of a rape victim could be influenced by exposure to negative social reactions: students indicated less willingness to provide sympathy and support to a hypothetical rape victim when they learned she had been blamed and stigmatized. The current study, which utilized a sample of 100 college students, replicated and extended these results and showed that men were affected by others' negative social reactions in their hypothetical judgments and in their behavioral responses to a rape victim (sitting farther away). This study demonstrates the potentially far-reaching detrimental influence of negative social reactions. [ABSTRACT FROM AUTHOR]

Students with autism spectrum disorder have been found to experience difficulty with reading comprehension despite intact decoding and word recognition. This identified need for targeted reading comprehension remediation results in a need for teachers to utilize research-based practices and to individualize instruction for students with autism spectrum disorder; however, teachers report a lack of access to such practices. This study utilized survey methodology to gather perceptions and experiences of teachers and to compare teacher preparedness to use effective instructional practices emerging from the extant research to teacher-reported effective practices in the classroom. Study findings, based on 112 participants, reveal a discrepancy between teacher-reported effective practices, and the practices identified as effective through research, indicating a research to practice gap. Implications for practice include professional development recommendations, and the need for increased communication between researchers and teachers. [ABSTRACT FROM AUTHOR]

Gossypol, a secondary plant compound found in cotton (Gossypium spp.), is known to be toxic to a variety of animals, particularly monogastric mammals and birds. Because ruminants are resistant to gossypol, whole cottonseed and cottonseed meal have been used as a feed supplement for many decades. Concerns over gossypol toxicity arise because of its presence in cottonseed products, particularly livestock and wildlife feed. The concentration of cottonseed in the environment near livestock and wildlife feeding stations presents the possibility that it may be ingested in significant amounts by non-target wild animals, resulting in inadvertent gossypol dosing. A species of significant economic and cultural value is the Northern Bobwhite (Colinus virginianus). However, there are no published data regarding if Northern Bobwhites would consume cottonseed meal given the opportunity. We assessed selection for or against cottonseed meal by performing a dietary preference study. Birds (n=120) were given the choice between commercial game bird feed, scratch grains, and cottonseed meal containing 0.74% total gossypol. We measured feed consumption for five days. The birds' diet consisted of 51.74% game bird feed, 37.72% scratch grains, and 10.54% cottonseed meal, and percentages varied significantly among all feed types (P≤0.001, n=360). We concluded that Northern Bobwhites selected against the cottonseed meal in favour of the game bird feed first and the scratch grains second. Our results suggest that given the choice, Northern Bobwhites will select against cottonseed meal if other feed choices are available. [ABSTRACT FROM AUTHOR]