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Background: Neuroimmune communication plays an important role in allergic inflammation, but the neuroimmune regulation of allergic rhinitis remains unclear., Objective: The goal of this study was to investigate the role of CD4-positive T lymphocyte (CD4 + T cells) adhesion to D-U87 neuron-like cells in mediating allergic rhinitis CD4 + T cell differentiation., Methods: D-U87 neuron-like cells were derived from the human glioblastoma U87 cell line. CD4 + T cells were isolated from human peripheral blood using a magnetic separation technique. In vitro coculture of D-U87 neuron-like cells and CD4 + T cells was established. The number of adherent CD4 + T cells was counted using a fluorescence microscope. The percentages of CD4 + IFNγ + and CD4 + IL4 + T cells and the levels of IFNγ and IL4 cytokines in the supernatant were measured by flow cytometry., Results: The results showed that the number of adherent CD4 + T cells in patients with allergic rhinitis was significantly higher than that in healthy controls. In allergic rhinitis, the percentage of CD4 + IL4 + T cells was significantly increased in the adherent group compared with that in the nonadherent group. Moreover, blocking ICAM1 and E-selectin decreased the number of adherent CD4 + T cells and the percentage of CD4 + IL4 + T cells in allergic rhinitis., Conclusion: Adhesion contributes to CD4 + IL4 + T cell differentiation in the in vitro coculture system of D-U87 neuron-like cells and allergic rhinitis CD4 + T cells, which may provide new insights into therapeutic strategies for allergic rhinitis., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.