Adhesion Promotes Allergic Rhinitis CD4 + IL4 + T Cell Differentiation via ICAM1 and E-Selectin.

Background: Neuroimmune communication plays an important role in allergic inflammation, but the neuroimmune regulation of allergic rhinitis remains unclear.
Objective: The goal of this study was to investigate the role of CD4-positive T lymphocyte (CD4 ⁺ T cells) adhesion to D-U87 neuron-like cells in mediating allergic rhinitis CD4 ⁺ T cell differentiation.
Methods: D-U87 neuron-like cells were derived from the human glioblastoma U87 cell line. CD4 ⁺ T cells were isolated from human peripheral blood using a magnetic separation technique. In vitro coculture of D-U87 neuron-like cells and CD4 ⁺ T cells was established. The number of adherent CD4 ⁺ T cells was counted using a fluorescence microscope. The percentages of CD4 ⁺ IFNγ ⁺ and CD4 ⁺ IL4 ⁺ T cells and the levels of IFNγ and IL4 cytokines in the supernatant were measured by flow cytometry.
Results: The results showed that the number of adherent CD4 ⁺ T cells in patients with allergic rhinitis was significantly higher than that in healthy controls. In allergic rhinitis, the percentage of CD4 ⁺ IL4 ⁺ T cells was significantly increased in the adherent group compared with that in the nonadherent group. Moreover, blocking ICAM1 and E-selectin decreased the number of adherent CD4 ⁺ T cells and the percentage of CD4 ⁺ IL4 ⁺ T cells in allergic rhinitis.
Conclusion: Adhesion contributes to CD4 ⁺ IL4 ⁺ T cell differentiation in the in vitro coculture system of D-U87 neuron-like cells and allergic rhinitis CD4 ⁺ T cells, which may provide new insights into therapeutic strategies for allergic rhinitis.
Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.