1. High density SNP mapping and QTL analysis for time of leaf budburst in Corylus avellana L
- Author
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Ezio Portis, Todd C. Mockler, Erik R. Rowley, Nadia Valentini, Alberto Acquadro, Daniela Torello Marinoni, Chiara Beltramo, Shawn A. Mehlenbacher, and Roberto Botta
- Subjects
Genetics and Molecular Biology (all) ,0106 biological sciences ,0301 basic medicine ,Leaves ,Genetic Linkage ,lcsh:Medicine ,Chromosomal translocation ,Plant Science ,Plant Genetics ,01 natural sciences ,Loss of heterozygosity ,DNA library construction ,Plant Genomics ,lcsh:Science ,Genetics ,education.field_of_study ,Multidisciplinary ,Chromosome Biology ,Plant Anatomy ,Chromosome Mapping ,Agriculture ,Genomics ,Genomic Library Construction ,Chromosomal Aberrations ,Phenotype ,Research Article ,Biotechnology ,Genetic Markers ,Quantitative Trait Loci ,Population ,Single-nucleotide polymorphism ,DNA construction ,Biology ,Quantitative trait locus ,Research and Analysis Methods ,Polymorphism, Single Nucleotide ,Chromosomes, Plant ,Molecular Genetics ,03 medical and health sciences ,Corylus ,Gene mapping ,Genetic linkage ,Molecular Biology Techniques ,Linkage Mapping ,education ,Molecular Biology ,Linkage (software) ,Agricultural and Biological Sciences (all) ,Gene Mapping ,lcsh:R ,Biology and Life Sciences ,Cell Biology ,Sequence Analysis, DNA ,Plant Leaves ,Plant Breeding ,030104 developmental biology ,Genetic Loci ,Chromosomal Translocations ,Plant Biotechnology ,lcsh:Q ,010606 plant biology & botany - Abstract
The growing area of European hazelnut (Corylus avellana L.) is increasing, as well as the number of producing countries, and there is a pressing need for new improved cultivars. Hazelnut conventional breeding process is slow, due to the length of juvenile phase and the high heterozygosity level. The development of genetic linkage maps and the identification of molecular markers tightly linked to QTL (quantitative trait loci) of agronomic interest are essential tools for speeding up the selection of seedlings carrying desired traits through marker-assisted selection. The objectives of this study were to enrich a previous linkage map and confirm QTL related to time of leaf budburst, using an F1 population obtained by crossing Tonda Gentile delle Langhe with Merveille de Bollwiller. Genotyping-by-Sequencing was used to identify a total of 9,999 single nucleotide polymorphism markers. Well saturated linkage maps were constructed for each parent using the double pseudo-testcross mapping strategy. A reciprocal translocation was detected in Tonda Gentile delle Langhe between two non-homologous chromosomes. Applying a bioinformatic approach, we were able to disentangle 'pseudo-linkage' between markers, removing markers around the translocation breakpoints and obtain a linear order of the markers for the two chromosomes arms, for each linkage group involved in the translocation. Twenty-nine QTL for time of leaf budburst were identified, including a stably expressed region on LG_02 of the Tonda Gentile delle Langhe map. The stability of these QTL and their coding sequence content indicates promise for the identification of specific chromosomal regions carrying key genes involved in leaf budburst.
- Published
- 2018