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High density SNP mapping and QTL analysis for time of leaf budburst in Corylus avellana L
- Source :
- PLoS ONE, Vol 13, Iss 4, p e0195408 (2018), PLoS ONE
- Publication Year :
- 2018
- Publisher :
- Public Library of Science (PLoS), 2018.
-
Abstract
- The growing area of European hazelnut (Corylus avellana L.) is increasing, as well as the number of producing countries, and there is a pressing need for new improved cultivars. Hazelnut conventional breeding process is slow, due to the length of juvenile phase and the high heterozygosity level. The development of genetic linkage maps and the identification of molecular markers tightly linked to QTL (quantitative trait loci) of agronomic interest are essential tools for speeding up the selection of seedlings carrying desired traits through marker-assisted selection. The objectives of this study were to enrich a previous linkage map and confirm QTL related to time of leaf budburst, using an F1 population obtained by crossing Tonda Gentile delle Langhe with Merveille de Bollwiller. Genotyping-by-Sequencing was used to identify a total of 9,999 single nucleotide polymorphism markers. Well saturated linkage maps were constructed for each parent using the double pseudo-testcross mapping strategy. A reciprocal translocation was detected in Tonda Gentile delle Langhe between two non-homologous chromosomes. Applying a bioinformatic approach, we were able to disentangle 'pseudo-linkage' between markers, removing markers around the translocation breakpoints and obtain a linear order of the markers for the two chromosomes arms, for each linkage group involved in the translocation. Twenty-nine QTL for time of leaf budburst were identified, including a stably expressed region on LG_02 of the Tonda Gentile delle Langhe map. The stability of these QTL and their coding sequence content indicates promise for the identification of specific chromosomal regions carrying key genes involved in leaf budburst.
- Subjects :
- Genetics and Molecular Biology (all)
0106 biological sciences
0301 basic medicine
Leaves
Genetic Linkage
lcsh:Medicine
Chromosomal translocation
Plant Science
Plant Genetics
01 natural sciences
Loss of heterozygosity
DNA library construction
Plant Genomics
lcsh:Science
Genetics
education.field_of_study
Multidisciplinary
Chromosome Biology
Plant Anatomy
Chromosome Mapping
Agriculture
Genomics
Genomic Library Construction
Chromosomal Aberrations
Phenotype
Research Article
Biotechnology
Genetic Markers
Quantitative Trait Loci
Population
Single-nucleotide polymorphism
DNA construction
Biology
Quantitative trait locus
Research and Analysis Methods
Polymorphism, Single Nucleotide
Chromosomes, Plant
Molecular Genetics
03 medical and health sciences
Corylus
Gene mapping
Genetic linkage
Molecular Biology Techniques
Linkage Mapping
education
Molecular Biology
Linkage (software)
Agricultural and Biological Sciences (all)
Gene Mapping
lcsh:R
Biology and Life Sciences
Cell Biology
Sequence Analysis, DNA
Plant Leaves
Plant Breeding
030104 developmental biology
Genetic Loci
Chromosomal Translocations
Plant Biotechnology
lcsh:Q
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....24e22bd86ecabc573ff5059ada5dd87f