1. A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation
- Author
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Paul Flicek, Rosalind H. Morley, Kim Lachani, Fiona C. Wardle, Michael J. Gilchrist, Damian Keefe, and James C. Smith
- Subjects
Fetal Proteins ,Brachyury ,animal structures ,Transcription, Genetic ,Molecular Sequence Data ,Gene regulatory network ,Mesoderm ,Animals ,Cell Lineage ,Gene Regulatory Networks ,Binding site ,Gene ,Transcription factor ,Zebrafish ,Conserved Sequence ,Body Patterning ,Homeodomain Proteins ,Genetics ,Binding Sites ,Multidisciplinary ,Base Sequence ,biology ,Muscles ,Gastrulation ,Gene Expression Regulation, Developmental ,Zebrafish Proteins ,Biological Sciences ,biology.organism_classification ,Cell biology ,embryonic structures ,Mesoderm formation ,T-Box Domain Proteins ,Chromatin immunoprecipitation ,Protein Binding ,Transcription Factors - Abstract
Using chromatin immunoprecipitation combined with genomic microarrays we have identified targets of No tail (Ntl), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We show that Ntl regulates a downstream network of other transcription factors and identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. We show that the notochord-expressed gene floating head ( flh ) is a direct transcriptional target of Ntl and that a combination of TBSs in the flh upstream region are required for Ntl-directed expression. Using our genome-scale data we have assembled a preliminary gene regulatory network that begins to describe mesoderm formation and patterning in the early zebrafish embryo.
- Published
- 2009