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A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation
- Source :
- Proceedings of the National Academy of Sciences. 106:3829-3834
- Publication Year :
- 2009
- Publisher :
- Proceedings of the National Academy of Sciences, 2009.
-
Abstract
- Using chromatin immunoprecipitation combined with genomic microarrays we have identified targets of No tail (Ntl), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We show that Ntl regulates a downstream network of other transcription factors and identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. We show that the notochord-expressed gene floating head ( flh ) is a direct transcriptional target of Ntl and that a combination of TBSs in the flh upstream region are required for Ntl-directed expression. Using our genome-scale data we have assembled a preliminary gene regulatory network that begins to describe mesoderm formation and patterning in the early zebrafish embryo.
- Subjects :
- Fetal Proteins
Brachyury
animal structures
Transcription, Genetic
Molecular Sequence Data
Gene regulatory network
Mesoderm
Animals
Cell Lineage
Gene Regulatory Networks
Binding site
Gene
Transcription factor
Zebrafish
Conserved Sequence
Body Patterning
Homeodomain Proteins
Genetics
Binding Sites
Multidisciplinary
Base Sequence
biology
Muscles
Gastrulation
Gene Expression Regulation, Developmental
Zebrafish Proteins
Biological Sciences
biology.organism_classification
Cell biology
embryonic structures
Mesoderm formation
T-Box Domain Proteins
Chromatin immunoprecipitation
Protein Binding
Transcription Factors
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 106
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....8291ca77ecd368f4da1f5b27f06460b6