1. Structure of the 70S ribosome from human pathogenStaphylococcus aureus
- Author
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Pascale Romby, Anthony Bochler, Quentin Vicens, François Grosse, I. Khusainov, Yaser Hashem, Stefano Marzi, Gulnara Yusupova, Jean-François Ménétret, A. Myasnikov, Johana Chicher, Marat Yusupov, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Architecture et réactivité de l'ARN (ARN), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Plateforme Protéomique Strasbourg Esplanade, Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), MATHELIN, Sandra, Architecture et Réactivité de l'ARN (ARN), and Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Protein Conformation, alpha-Helical ,0301 basic medicine ,[SDV]Life Sciences [q-bio] ,Gene Expression ,Human pathogen ,[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,medicine.disease_cause ,Ribosome ,0302 clinical medicine ,Structural Biology ,Translational regulation ,Pathogen ,ComputingMilieux_MISCELLANEOUS ,0303 health sciences ,Translation (biology) ,[SDV] Life Sciences [q-bio] ,RNA, Bacterial ,Staphylococcus aureus ,Corrigendum ,Bacillus subtilis ,Protein Binding ,Ribosomal Proteins ,Computational biology ,Molecular Dynamics Simulation ,Biology ,Microbiology ,03 medical and health sciences ,Antibiotic resistance ,Bacterial Proteins ,Species Specificity ,Escherichia coli ,[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Genetics ,medicine ,Protein Interaction Domains and Motifs ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Amino Acid Sequence ,030304 developmental biology ,Binding Sites ,Sequence Homology, Amino Acid ,Thermus thermophilus ,Cryoelectron Microscopy ,RNA ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,030104 developmental biology ,Protein Biosynthesis ,Nucleic Acid Conformation ,Protein Conformation, beta-Strand ,Ribosomes ,Sequence Alignment ,030217 neurology & neurosurgery - Abstract
Comparative structural studies of ribosomes from various organisms keep offering exciting insights on how species-specific or environment-related structural features of ribosomes may impact translation specificity and its regulation. Although the importance of such features may be less obvious within more closely related organisms, their existence could account for vital yet species-specific mechanisms of translation regulation that would involve stalling, cell survival and antibiotic resistance. Here, we present the first full 70S ribosome structure from Staphylococcus aureus, a Gram-positive pathogenic bacterium, solved by cryo-electron microscopy. Comparative analysis with other known bacterial ribosomes pinpoints several unique features specific to S. aureus around a conserved core, at both the protein and the RNA levels. Our work provides the structural basis for the many studies aiming at understanding translation regulation in S. aureus and for designing drugs against this often multi-resistant pathogen.
- Published
- 2016
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