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Motivation: Two-dimensional [15N-1H] separated local field solid-state nuclear magnetic resonance (NMR) experiments of membrane proteins aligned in lipid bilayers provide tilt and rotation angles for α-helical segments using Polar Index Slant Angle (PISA)-wheel models. No integrated software has been made available for data analysis and visualization., Results: We have developed the PISA-SPARKY plugin to seamlessly integrate PISA-wheel modeling into the NMRFAM-SPARKY platform. The plugin performs basic simulations, exhaustive fitting against experimental spectra, error analysis and dipolar and chemical shift wave plotting. The plugin also supports PyMOL integration and handling of parameters that describe variable alignment and dynamic scaling encountered with magnetically aligned media, ensuring optimal fitting and generation of restraints for structure calculation., Availability and Implementation: PISA-SPARKY is freely available in the latest version of NMRFAM-SPARKY from the National Magnetic Resonance Facility at Madison (http://pine.nmrfam.wisc.edu/download_packages.html), the NMRbox Project (https://nmrbox.org) and to subscribers of the SBGrid (https://sbgrid.org). The pisa.py script is available and documented on GitHub (https://github.com/weberdak/pisa.py) along with a tutorial video and sample data., Supplementary Information: Supplementary data are available at Bioinformatics online., (© The Author(s) 2020. Published by Oxford University Press.)

The lipophilic ligand-bridged dinuclear cation Rubb₁₆ is significantly cytotoxic and preferentially accumulates in the mitochondria of the L1210 murine leukemia cancer cell line.

Frequent relocations have historically been viewed negatively and are perceived to lead to aberrant behavior. Military adolescents are a highly mobile population with a highly variable number of relocations. This study assessed parental perceptions of military adolescents' conduct and behavior in the context of relocation experience. Parents of military adolescents were surveyed for their children's history of conduct and behavior, with 179 completed surveys being returned from geographically separate sites. The average number of relocations experienced by the adolescents was 4.89. Parental perceptions of relocations improved with the number of relocations experienced (p < 0.05). As more relocations were experienced, children's behavior improved (p < 0.05), when controlling for age. The data suggested that relocation frequency was a more predictive measure of improved parental perceptions and decreased aberrant behavior. Data from this study suggest that relocation frequency may be a more critical factor in resilience development than the actual number of relocations experienced.