1. Electron Microscopy as a Tool for Assessment of Anticoagulation Strategies During Extracorporeal Life Support: The Proof Is on the Membrane
- Author
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Kevin K. Chung, Andrew P. Cap, Kathryn A Negaard, Leopoldo C. Cancio, Brendan M Beely, Andrew D. Meyer, Andriy I. Batchinsky, James E. Campbell, Thomas Langer, Beely, B, Campbell, J, Meyer, A, Langer, T, Negaard, K, Chung, K, Cap, A, Cancio, L, and Batchinsky, A
- Subjects
endocrine system ,medicine.medical_specialty ,ARDS ,medicine.medical_treatment ,Biomedical Engineering ,Biophysics ,Pilot Projects ,Bioengineering ,030204 cardiovascular system & hematology ,extracorporeal life support ,Article ,Extracorporeal ,Biomaterials ,03 medical and health sciences ,Extracorporeal Membrane Oxygenation ,0302 clinical medicine ,Bolus (medicine) ,Extracorporeal membrane oxygenation ,medicine ,Animals ,anticoagulation ,medicine (all) ,Oxygenators, Membrane ,Retrospective Studies ,Mechanical ventilation ,Respiratory Distress Syndrome ,Sheep ,Heparin ,business.industry ,biomaterial ,Anticoagulants ,Thrombosis ,030208 emergency & critical care medicine ,General Medicine ,acute respiratory distress syndrome ,medicine.disease ,biophysic ,Surgery ,Life support ,Microscopy, Electron, Scanning ,business ,medicine.drug - Abstract
Extracorporeal life support (ECLS) is fast becoming more common place for use in adult patients failing mechanical ventilation. Management of coagulation and thrombosis has long been a major complication in the use of ECLS therapies. Scanning electron microscopy (SEM) of membrane oxygenators (MOs) after use in ECLS circuits can offer novel insight into any thrombotic material deposition on the MO. In this pilot study, we analyzed five explanted MOs immediately after use in a sheep model of different acute respiratory distress syndrome (ARDS). We describe our methods of MO dissection, sample preparation, image capture, and results. Of the five MOs analyzed, those that received continuous heparin infusion showed very little thrombosis formation or other clot material, whereas those that were used with only initial heparin bolus showed readily apparent thrombotic material.
- Published
- 2016
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