Back to Search
Start Over
Electron Microscopy as a Tool for Assessment of Anticoagulation Strategies During Extracorporeal Life Support: The Proof Is on the Membrane
- Source :
- ASAIO J
- Publication Year :
- 2016
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2016.
-
Abstract
- Extracorporeal life support (ECLS) is fast becoming more common place for use in adult patients failing mechanical ventilation. Management of coagulation and thrombosis has long been a major complication in the use of ECLS therapies. Scanning electron microscopy (SEM) of membrane oxygenators (MOs) after use in ECLS circuits can offer novel insight into any thrombotic material deposition on the MO. In this pilot study, we analyzed five explanted MOs immediately after use in a sheep model of different acute respiratory distress syndrome (ARDS). We describe our methods of MO dissection, sample preparation, image capture, and results. Of the five MOs analyzed, those that received continuous heparin infusion showed very little thrombosis formation or other clot material, whereas those that were used with only initial heparin bolus showed readily apparent thrombotic material.
- Subjects :
- endocrine system
medicine.medical_specialty
ARDS
medicine.medical_treatment
Biomedical Engineering
Biophysics
Pilot Projects
Bioengineering
030204 cardiovascular system & hematology
extracorporeal life support
Article
Extracorporeal
Biomaterials
03 medical and health sciences
Extracorporeal Membrane Oxygenation
0302 clinical medicine
Bolus (medicine)
Extracorporeal membrane oxygenation
medicine
Animals
anticoagulation
medicine (all)
Oxygenators, Membrane
Retrospective Studies
Mechanical ventilation
Respiratory Distress Syndrome
Sheep
Heparin
business.industry
biomaterial
Anticoagulants
Thrombosis
030208 emergency & critical care medicine
General Medicine
acute respiratory distress syndrome
medicine.disease
biophysic
Surgery
Life support
Microscopy, Electron, Scanning
business
medicine.drug
Subjects
Details
- ISSN :
- 10582916
- Volume :
- 62
- Database :
- OpenAIRE
- Journal :
- ASAIO Journal
- Accession number :
- edsair.doi.dedup.....1da781fd4dda446a20301f2daf3b962e
- Full Text :
- https://doi.org/10.1097/mat.0000000000000394