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Electron Microscopy as a Tool for Assessment of Anticoagulation Strategies During Extracorporeal Life Support: The Proof Is on the Membrane

Authors :
Kevin K. Chung
Andrew P. Cap
Kathryn A Negaard
Leopoldo C. Cancio
Brendan M Beely
Andrew D. Meyer
Andriy I. Batchinsky
James E. Campbell
Thomas Langer
Beely, B
Campbell, J
Meyer, A
Langer, T
Negaard, K
Chung, K
Cap, A
Cancio, L
Batchinsky, A
Source :
ASAIO J
Publication Year :
2016
Publisher :
Ovid Technologies (Wolters Kluwer Health), 2016.

Abstract

Extracorporeal life support (ECLS) is fast becoming more common place for use in adult patients failing mechanical ventilation. Management of coagulation and thrombosis has long been a major complication in the use of ECLS therapies. Scanning electron microscopy (SEM) of membrane oxygenators (MOs) after use in ECLS circuits can offer novel insight into any thrombotic material deposition on the MO. In this pilot study, we analyzed five explanted MOs immediately after use in a sheep model of different acute respiratory distress syndrome (ARDS). We describe our methods of MO dissection, sample preparation, image capture, and results. Of the five MOs analyzed, those that received continuous heparin infusion showed very little thrombosis formation or other clot material, whereas those that were used with only initial heparin bolus showed readily apparent thrombotic material.

Details

ISSN :
10582916
Volume :
62
Database :
OpenAIRE
Journal :
ASAIO Journal
Accession number :
edsair.doi.dedup.....1da781fd4dda446a20301f2daf3b962e
Full Text :
https://doi.org/10.1097/mat.0000000000000394