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The article informs that LCGC, the leading resource for separation scientists, has bestowed Ronald E. Majors and Zachary S. Breitbach as the winners of the 11th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively.

Aggregated singletons for automated purification (ASAP) is a purification workf low that provides singleton sample purification, registration, and delivery to the materials management department as 30 mM dimethyl sulfoxide solutions for biological screening. The singleton samples submitted are aggregated in a mini-array of 10-12 samples and then are analyzed using an automated purification process. The steps in the process include pre-quality control (QC), preparative chromatography, solvent evaporation, reformat dilution and duplication, final QC, and final registration. The turnaround time from samples received to delivery to materials management is two or three business days. The final QC data are uploaded to a database and are available to chemists via electronic laboratory notebook software. The final purity, weight recovery, and registration information is available in a research database and an e-mail notification of completion is sent to the chemist. ASAP enables a high purification success rate, increases the likelihood of running mini-arrays that generate 5-10 analogs in a final step rather than one or two with the same 2-3 day turnaround time, and provides expert-level service and technology. [ABSTRACT FROM AUTHOR]

The article discusses the methodology for using amide hydrogen-deuterium exchange with chromatographic materials as a means to identify buffer and resin conditions that are destabilizing to specific proteins. It is found that amide hydrogen-deuterium exchange is a powerful tool for fast resin screening during purification process development.

Focuses on the introduction of large numbers of columns and packing materials designed for preparative liquid chromatography during the Pittcon conference in the U.S. Basics of preparative chromatography; Exploration of the use of column in preparative high performance liquid chromatography; Goal of a preparactive purification.

The article highlights the research on the effectiveness of XSelect Columns and Focused Gradients in isolating and purifying ranitidine in the U.S. The research shows that ranitidine has better retention under high percentage of Hydrogen (pH) mobile phase conditions using ammonium hydroxide. Moreover, the method can directly transfer chromatographic separations between the analytical and preparative column dimensions.

The article examines the benefits of utilizing flash or high performance liquid chromatography (HPLC) for compound purification. It discusses several factors to consider before starting a liquid chromatography purification of a target compound which includes difficulty of the separation, amount of compound needed and available instrumentation. It concludes that the purifications do not require high efficiency and added that flash is a viable alternative to HPLC for compound purification.

The article discusses a study on the flash purification of δ-tocopherol and high performance liquid chromatography (HPLC) analysis for sample purity. It defines HPLC as a method using small particle media, while flash purification as a technique using larger particle media. It discovers that chromatographic separations can be transferred between HPLC and flash purification and both methods save time and money.

The article focuses on the investigation of the routine switching between high and low pH on a single XBridge high performance liquid chromatography (HPLC) column. The ability to switch between pH extremes on a single HPLC column can reduce the time for separation of pharmaceutical compounds. After several cycles of exposure to high and low pH, no deterioration was seen which makes XBridge an ideal choice for the routine screening, method development and purification of pharmaceutical compounds.

The article features the Flash Chromatography, a popular method that separates organic compounds through purification. The technique is used to scale up normal phase chemistries from thin layer chromatography. It also extends a wide range of applications, which includes purification of small compounds, peptides, and purification of natural products.

Demonstrates the application of the cLC System from Gilson Inc. as a single chromatography system capable of automating the purification of compounds for reverse and normal phase preparations. Ability of the system to accommodate various detection modes; Automation of on-line evaluation of collected fractions through integration of analytical components into the system; Possibility of handling five columns.