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The use of alternative diesel fuels has increased due to the demand for renewable energy sources. There is limited knowledge regarding the potential health effects caused by exhaust emissions from biodiesel- and renewable diesel-fueled engines. This study investigates the toxic effects of particulate matter (PM) emissions from a diesel engine powered by conventional petroleum diesel fuel (SD10) and two biodiesel and renewable diesel fuels in vitro. The fuels used were rapeseed methyl ester (RME), soy methyl ester (SME), and Hydrogenated Vegetable Oil (HVO), either pure or as 50% blends with SD10. Additionally, a 5% RME blend was also used. The highest concentration of polycyclic aromatic hydrocarbon emissions and elemental carbon (EC) was found in conventional diesel and the 5% RME blend. HVO PM samples also exhibited a high amount of EC. A dose-dependent genotoxic response was detected with PM from SD10, pure SME, and RME as well as their blends. Reactive oxygen species levels were several times higher in cells exposed to PM from SD10, pure HVO, and especially the 5% RME blend. Apoptotic cell death was observed in cells exposed to PM from SD10, 5% RME blend, the 50% SME blend, and HVO samples. In conclusion, all diesel PM samples, including biodiesel and renewable diesel fuels, exhibited toxicity.

An experimental investigation is performed in a single-cylinder direct-injection (DI) diesel engine using city diesel oil called DI1 and two blends of DI1 with a mixture of glycol ethers. The addition of glycol ethers to fuel DI1 produced oxygenated fuels GLY10 (10.2 mass-% glycol ethers) and GLY30 (31.3 mass-% glycol ethers) with 3% and 9% oxygen content, respectively. The addition of biofuel rapeseed methyl ester (RME) to fuel DI1 produced oxygenated blend RME30 (31.2 mass-% RME) with 3% oxygen content. Engine tests were performed with the four fuels in the DI diesel engine at 2500 RPM and at 20%, 40%, 60%, and 80% of full load. The experimental diesel engine was equipped with devices for recording cylinder pressure, injection pressure, and top dead center (TDC) position and also it was equipped with exhaust gas analyzers for measuring soot, NO, CO, and HC emissions. A MATLAB 2014 code was developed for analyzing recorded cylinder pressure, injection pressure, and TDC position data for all obtained engine cycles and for calculating the main engine performance parameters. The assessment of the experimental results showed that glycol ethers have more beneficial impact on soot and NO emissions compared to RME, whereas RME have less detrimental impact on engine performance parameters compared to glycol ethers.