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Background: The standard of care for stage 1 NSCLC is upfront surgery followed by surveillance. However, 20–30% of stage 1 NSCLC recur. There is an unmet need to identify individuals likely to recur who would benefit from frequent monitoring and aggressive cancer treatments. Collagen 1 (Col1) fibers detected by second harmonic generation (SHG) microscopy are a major structural component of the extracellular matrix (ECM) of tumors that play a role in cancer progression. Method: We characterized Col1 fibers with SHG microscopy imaging of surgically resected stage 1 NSCLC. Gene expression from RNA sequencing data was used to validate the SHG microscopy findings. Results: We identified a significant (p ≤ 0.05) increase in the Col1 fiber volume in stage 1 NSCLC that recurred. The increase in Col1 fiber volume was supported by significant increases in the gene expression of Col1 in invasive, compared to noninvasive, lung adenocarcinoma. Significant differences were identified in the gene expression of other ECM proteins, as well as CAFs, immune checkpoint markers, immune cytokines, and T-cell markers. Conclusion: Col1 fiber analysis can provide a companion diagnostic test to evaluate the likelihood of tumor recurrence following stage 1 NSCLC. The studies expand our understanding of the role of the ECM in NSCLC recurrence.

Uterine corpus endometrial carcinoma (UCEC) poses significant clinical challenges due to its high incidence and poor prognosis, exacerbated by the lack of effective screening methods. The standard treatment for UCEC typically involves surgical intervention, with radiation and chemotherapy as potential adjuvant therapies. In recent years, immunotherapy has emerged as a promising avenue for the advanced treatment of UCEC. This study employs a multi-omics approach, analyzing RNA-sequencing data and clinical information from The Cancer Genome Atlas (TCGA), Gene Expression Profiling Interactive Analysis (GEPIA), and GeneMANIA databases to investigate the prognostic value of MutL Homolog 1 (MLH1) gene expression in UCEC. The dysregulation of MLH1 in UCEC is linked to adverse prognostic outcomes and suppressed immune cell infiltration. Gene Set Enrichment Analysis (GSEA) data reveal MLH1’s involvement in immune-related processes, while its expression correlates with tumor mutational burden (TMB) and microsatellite instability (MSI). Lower MLH1 expression is associated with poorer prognosis, reduced responsiveness to Programmed cell death protein 1 (PD-1)/Programmed death-ligand 1 (PD-L1) inhibitors, and heightened sensitivity to anti-cancer agents. This comprehensive analysis establishes MLH1 as a potential biomarker for predicting prognosis, immunotherapy response, and drug sensitivity in UCEC, offering crucial insights for the clinical management of patients.

The solid backfilling mining method is one of the methods used to solve problems arising from strata and ground subsidence in underground mines. Through 2D physical analog modeling, 3D numerical simulation, and field measurement, the effects of the solid backfilling method were analyzed, providing a better insight into optimizing the configurations of a working face beneath a city for safety, environmental problems, and its use in production. In the physical modeling, MatchID software was employed to capture the movement characteristics of overlying strata and ground subsidence during mining and backfilling. Key parameters such as vertical displacement, subsidence characteristics, and rock mass stress variations were monitored and analyzed. In the numerical simulation, FLAC3D was used to simulate and analyze the effect of the backfill body on strata and ground subsidence above the backfill working face. For the field measurements, the Continuously Operating Reference Station (CORS) system was used to confirm the effective control of ground subsidence. With a filling ratio of 80%, the three methods are consistent and show a maximum subsidence value of 0.46 mm (physical simulation), 50.4 mm (numerical simulation), and 47 mm (experienced), significantly lower than the predicted subsidence, which is 281 mm. Therefore, this study demonstrates the reliability and scientific validity of both the physical analog modeling method and the field measurement method in measuring the efficiency of solid backfilling, providing valuable insights into strata and ground subsidence control in longwall coal mining.

Obesity is a multifactorial chronic inflammatory metabolic disorder, with pathogenesis influenced by genetic and non-genetic factors such as environment and diet. Intestinal microbes and their metabolites play significant roles in the occurrence and development of obesity by regulating energy metabolism, inducing chronic inflammation, and impacting intestinal hormone secretion. Epigenetics, which involves the regulation of host gene expression without changing the nucleotide sequence, provides an exact direction for us to understand how the environment, lifestyle factors, and other risk factors contribute to obesity. DNA methylation, as the most common epigenetic modification, is involved in the pathogenesis of various metabolic diseases. The epigenetic modification of the host is induced or regulated by the intestinal microbiota and their metabolites, linking the dynamic interaction between the microbiota and the host genome. In this review, we examined recent advancements in research, focusing on the involvement of intestinal microbiota and DNA methylation in the etiology and progression of obesity, as well as potential interactions between the two factors, providing novel perspectives and avenues for further elucidating the pathogenesis, prevention, and treatment of obesity.

Skeleton-based action recognition, renowned for its computational efficiency and indifference to lighting variations, has become a focal point in the realm of motion analysis. However, most current methods typically only extract global skeleton features, overlooking the potential semantic relationships among various partial limb motions. For instance, the subtle differences between actions such as “brush teeth” and “brush hair” are mainly distinguished by specific elements. Although combining limb movements provides a more holistic representation of an action, relying solely on skeleton points proves inadequate for capturing these nuances. Therefore, integrating detailed linguistic descriptions into the learning process of skeleton features is essential. This motivates us to explore integrating fine-grained language descriptions into the learning process of skeleton features to capture more discriminative skeleton behavior representations. To this end, we introduce a new Linguistic-Driven Partial Semantic Relevance Learning framework (LPSR) in this work. While using state-of-the-art large language models to generate linguistic descriptions of local limb motions and further constrain the learning of local motions, we also aggregate global skeleton point representations and textual representations (which generated from an LLM) to obtain a more generalized cross-modal behavioral representation. On this basis, we propose a cyclic attentional interaction module to model the implicit correlations between partial limb motions. Numerous ablation experiments demonstrate the effectiveness of the method proposed in this paper, and our method also obtains state-of-the-art results.

Sunflower is an important crop, and the vitality and moisture content of sunflower seeds have an important influence on the sunflower’s planting and yield. By employing hyperspectral technology, the spectral characteristics of sunflower seeds within the wavelength range of 384–1034 nm were carefully analyzed with the aim of achieving effective prediction of seed vitality and moisture content. Firstly, the original hyperspectral data were subjected to preprocessing techniques such as Savitzky–Golay smoothing, standard normal variable correction (SNV), and multiplicative scatter correction (MSC) to effectively reduce noise interference, ensuring the accuracy and reliability of the data. Subsequently, principal component analysis (PCA), extreme gradient boosting (XGBoost), and stacked autoencoders (SAE) were utilized to extract key feature bands, enhancing the interpretability and predictive performance of the data. During the modeling phase, random forests (RFs) and LightGBM algorithms were separately employed to construct classification models for seed vitality and prediction models for moisture content. The experimental results demonstrated that the SG-SAE-LightGBM model exhibited outstanding performance in the classification task of sunflower seed vitality, achieving an accuracy rate of 98.65%. Meanwhile, the SNV-XGBoost-LightGBM model showed remarkable achievement in moisture content prediction, with a coefficient of determination (R2) of 0.9715 and root mean square error (RMSE) of 0.8349. In conclusion, this study confirms that the fusion of hyperspectral technology and multivariate data analysis algorithms enables the accurate and rapid assessment of sunflower seed vitality and moisture content, providing robust tools and theoretical support for seed quality evaluation and agricultural production practices. Furthermore, this research not only expands the application of hyperspectral technology in unraveling the intrinsic vitality characteristics of sunflower seeds but also possesses significant theoretical and practical value.

Acute colitis is a complex disease that can lead to dysregulation of the gut flora, inducing more complex parenteral diseases. Dandelion polysaccharides (DPSs) may have potential preventive and therapeutic effects on enteritis. In this study, LPS was used to induce enteritis and VC was used as a positive drug control to explore the preventive and therapeutic effects of DPS on enteritis. The results showed that DPS could repair the intestinal barrier, down-regulate the expression of TNF-α, IL-6, IL-1β, and other pro-inflammatory factors, up-regulate the expression of IL-22 anti-inflammatory factor, improve the antioxidant capacity of the body, and improve the structure of intestinal flora. It is proved that DPS can effectively prevent and treat LPS-induced acute enteritis and play a positive role in promoting intestinal health.

Operational amplifiers (op-amps) are widely used in circuit systems. The increasing complexity of the power supply network has led to the susceptibility of the power supply port to electromagnetic interference (EMI) in circuit systems. Therefore, it is necessary to investigate the electromagnetic susceptibility (EMS) of op-amps at the power supply port. In this paper, we assessed the effect of EMI on the operational performance of op-amps through the power supply port by a bulk current injection (BCI) method. Firstly, we conducted the continuous sine wave into the power supply port by a current injection probe and measured the change in the offset voltage under EMI. Secondly, we proposed a new method of conducted susceptibility and obtained the susceptibility threshold regularities of the op-amps at the power supply port under the interference of different waveform signals. Our study provided conclusive evidence that EMI reduced the reliability of the op-amp by affecting the offset voltage of op-amps and demonstrated that the sensitivity type of op-amps was peak-sensitive at the power supply port. This study contributed to a deep understanding of the EMS mechanism and guided the design of electromagnetic compatibility (EMC) of op-amps.

China produces more than 30 million tons of drug residues every year. Therefore, innovative solutions are needed to mitigate environmental damage. Certain plant compounds boost hens’ health and performance. Radix isatidis is promising for layer production. This study elucidates the multidimensional impact of Radix isatidis residual material (RIHR) on laying hens, focusing on the egg quality, intestinal health and the microbial landscape. A total of 288 55-week-old Peking powder laying hens with similar laying rates and body weights were randomly divided into four groups, with eight replicates per group and nine hens per replicate. The groups were divided into a control group, an RIHR low-dose group, a medium-dose group and a high-dose group according to a single-factor, completely randomized design. For the three RIHR treatment groups, the added amounts were 5 kg/t, 10 kg/t and 15 kg/t, respectively. Liquid chromatography- mass spectrometry (LC-MS), molecular docking, fluorescence quantitative PCR and other methods were used. The results showed that three main anti-inflammatory and antiviral compounds were identified in RIHR-indirubin (0.21 μg/g), deoxyvasicinone (0.18 μg/g) and epigoitrin (0.39 μg/g). RIHR significantly increased the eggshell thickness, Haugh unit and protein height (p < 0.05). It also had significant antioxidant and anti-inflammatory effects on ilea and ceca (p < 0.05). The microbial analysis demonstrated that RIHR supplementation led to a significant reduction in foregut Lactobacillus levels (p < 0.05). In the hindgut, a significant increase in pathogenic bacteria was observed (p < 0.05). The study concludes that RIHR’s anti-inflammatory compounds may directly act on the intestinal tract to modulate inflammation, highlighting its potential for targeted interventions in poultry health and nutrition.

Debris poses multifaceted risks and jeopardizes various aspects of the environment, human health, safety, and infrastructure. The debris trajectory in turbulent wind flow is more dispersed due to the inherent randomness of the turbulent winds. This paper investigates the three-dimensional trajectories of plate-type wind-borne debris in turbulent wind fields via the method of numerical simulation. A 3D probabilistic trajectory model of plate-type wind-borne debris is developed. The debris trajectories are numerically calculated by solving the governing equation of debris motion and by introducing turbulent wind flows based on the near-ground wind field measured in the wind tunnel to account for the probability characteristics of the debris trajectory. The dimensionless velocities and displacements of the debris trajectory show good agreement with the experimental data in wind tunnel tests, confirming the rationality of the probabilistic trajectory model. Based on the validated trajectory model, the probability characteristics of the debris impact position, impact velocity, and kinetic energy, debris angular displacement, and angular velocity are analyzed in detail under five different wind attack angles. The proposed probabilistic model of plate-type debris in turbulent wind flow provides an accurate and effective method for predicting debris trajectory in three-dimensional space.

Target detection has always been a hotspot in image processing/computer vision research, and small-target detection is a frequently encountered problem in the field of target detection. With the continuous innovation of target detection technology, people always hope that the detection of small targets can reach the real-time accuracy of large-target detection. In this paper, a small-target detection model based on dual-core convolutional neural networks (CNN) is proposed, which is mainly used for the intelligent detection of books in the production line of printed books. The model is mainly composed of two modules, including a region prediction module and suspicious target search module. The region prediction module uses a CNN to predict suspicious region blocks in a large context. The suspicious target search module uses a different CNN from the above to find tiny targets in the predicted region blocks. Comparative testing of four small book target samples using this model shows that this model has better book small-target detection accuracy compared to other models.

In this study, we isolated and identified pathogenic fungi from the naturally occurring fruits of red grapes, studied their biological characteristics, screened fifteen essential oil components to find the best natural antibacterial agent with the strongest inhibitory effect, and then compared the incidence of postharvest diseases and storage potential of red grapes treated with two concentrations (0.5 EC50/EC50) of essential oil components (inoculated with pathogenic fungi) during storage for 12 d at room temperature. In our research, Alternaria alternata was the primary pathogenic fungus of red grapes. Specifically, red grapes became infected which caused diseases, regardless of whether they were inoculated with Alternaria alternata in an injured or uninjured state. Our findings demonstrated that the following conditions were ideal for Alternaria alternata mycelial development and spore germination: BSA medium, D-maltose, ammonium nitrate, 28 °C, pH 6, and exposure to light. For the best Alternaria alternata spore production, OA medium, mannitol, urea, 34 °C, pH 9, and dark conditions were advised. Furthermore, with an EC50 value of 36.71 μg/mL, carvacrol demonstrated the highest inhibitory impact on Alternaria alternata among the 15 components of essential oils. In the meantime, treatment with EC50 concentration of carvacrol was found to be more effective than 0.5 EC50 concentration for controlling Alternaria alternata-induced decay disease of red grapes. The fruits exhibited remarkable improvements in the activity of defense-related enzymes, preservation of the greatest hardness and total soluble solids content, reduction in membrane lipid peroxidation in the peel, and preservation of the structural integrity of peel cells. Consequently, carvacrol was able to prevent the Alternaria alternata infestation disease that affects red grapes, and its EC50 concentration produced the greatest outcomes.

Using the powder-metallurgy rolling method, aluminum foam sandwich (AFS) panels with a metallurgical bond between the foam core and the panel can be produced. In this study, by manipulating the foaming temperature and duration, AFS panels were fabricated with varying core densities and thicknesses, all maintaining a panel thickness close to 1 mm. Through the three-point bending test, this research deeply delved into how core density influences the mechanical behaviors of these AFS panels. It became evident that a rise in core density positively affects the bending strength and failure load of the panels but inversely impacts their total energy absorption efficiency. Differing core densities brought about distinct failure patterns: low-density samples primarily showed panel indentation and core shear failures, whereas those of high density demonstrated panel yield and fractures. Furthermore, the research offers predictions on the initial failure loads for different failure modes and introduces a comprehensively designed failure diagram, laying a foundational theory for the production of AFS panels.

Late Cryogenian–Ediacaran magmatism represents the latest Precambrian tectonothermal event in the Tarim Craton. However, its geodynamic setting and geological significance are controversial. Here, we report the geochronology, whole-rock geochemistry, and Sr-Nd-Hf isotopic compositions of newly identified late Cryogenian A-type and highly fractionated S-type granites from two locations in the northern Tarim Craton. LA-ICP-MS zircon U-Pb analyses yield ages of 642 ± 7 Ma for a syenogranite and 643 ± 4.5 Ma for a mylonitized granite. The syenogranite is weakly peraluminous and shows an A-type granite affinity, as indicated by its high K2O + Na2O contents (8.35–8.64 wt.%), high field strength elements (Zr + Nb + Ce + Y = 435.8 − 463.4 × 10−6), Ga/Al ratios (2.79−2.83), and zircon saturation temperatures (829–844 °C). In contrast, the mylonitized granite contains Al-oversaturated minerals (e.g., garnet) and has high a differentiation index (DI = of 98.9–99.4), with lower zircon saturation temperatures (786–792 °C); the samples display high SiO2 contents (72.99–74.00 wt.%) and A/CNK values (1.16–1.17) and low Nb/Ta and Zr/Hf ratios and are enriched in Rb and depleted in Ba, Sr, which all point to a highly fractionated S-type granite affinity. The granites are characterized by elevated large-ion lithosphere elements (LILEs) and flat high-field-strength elements (HFSEs) patterns, with deep Nb and Ta troughs and pronounced negative Eu anomalies (Eu/Eu* = 0.17–0.38). They show apparently negative εNd(t) values (−10.1 to −9.8 and −6.8 to −7.9, respectively) and εHf(t) values (−9.66 to −1.77 and −33.5 to −1.3, respectively) with Paleoproterozoic crustal model ages, indicating that they were mainly generated by the partial melting of mature crustal materials with a minor contribution from a mantle-derived magmatic source. By integrating with previously published geological, sedimentological, and structural data, we suggest that the granites formed due to a high-temperature gradient in a syn-subduction extensional setting that was probably induced by northward slab rollback of the Paleo-Asian Oceanic lithosphere. Our new data highlight an upper-plate extension in the northern Tarim Craton that constitutes the northern periphery of the Rodinia supercontinent. The linear distribution of late Cryogenian magmatic rocks provides critical evidence for the orogen strike extension of the terminal suture between the Tarim Craton and southwestern Altaids.

This study aimed to examine the relationship between daily total intake of water (DTIW) and handgrip strength (HGS) among US adults and to explore the impact of water intake on muscle function and health, providing a reference for public health policies and health education. Using the data from the National Health and Nutrition Examination Survey (NHANES) 2011–2014, a cross-sectional survey design was adopted to analyze 5427 adults (48.37% female and 51.63% male) aged 20 years and above. DTIW was assessed using two non-consecutive 24 h dietary recall interviews, and the HGS level was measured using a Takei Dynamometer. Weighted generalized linear regression models and restricted cubic spline plots were used to analyze the linear and nonlinear associations between DTIW and HGS level and to conduct a gender subgroup analysis and an interaction effect test. The results showed that there were significant differences in HGS and other characteristics among different quartile groups of DTIW (p < 0.05). There was a significant nonlinear trend (exhibiting an inverted U-curve) between DTIW and HGS (p for nonlinear = 0.0044), with a cut-off point of 2663 g/day. Gender subgroup analysis showed that the nonlinear trend (exhibiting an inverted U-curve) was significant only in males (p for nonlinear = 0.0016), with a cut-off point of 2595 g/day. None of the stratified variables had an interaction effect on the association between DTIW and HGS (p for interaction > 0.05). In conclusion, this study found a nonlinear association between DTIW and HGS levels, as well as a gender difference. This finding provides new clues and directions for exploring the mechanism of the impact of DTIW on muscle function and health and also provides new evidence and suggestions for adults to adjust their water intake reasonably.

Geohazards pose significant risks to communities and infrastructure, emphasizing the need for accurate susceptibility assessments to guide land-use planning and hazard management. This study presents a comprehensive method that combines Variable Weight Theory (VWT) with Analytic Hierarchy Process (AHP) to assess geo-environment vulnerability based on susceptibility to various geohazards. The method was applied to the Pearl River Delta in China, resulting in the classification of areas into high vulnerability (5961.85 km2), medium vulnerability (19,227.93 km2), low vulnerability (14,892.02 km2), and stable areas (1616.19 km2). The findings demonstrate improved accuracy and reliability compared to using AHP alone. ROC curve analysis confirms the enhanced performance of the integrated method, highlighting its effectiveness in discerning susceptibility levels and making informed decisions in hazard preparedness and risk reduction. Additionally, this study assessed the risks posed by geohazards to critical infrastructures, roads, and artificial surfaces, while discussing prevention strategies. However, this study acknowledges certain limitations, including the subjective determination of its judgment matrix and data constraints. Future research could explore the integration of alternative methods to enhance the objectivity of factor weighting. In practical applications, this study contributes to the understanding of geo-environment vulnerability assessments, providing insight into the intricate interplay among geological processes, human activities, and disaster resilience.

Coastal areas have made substantial contributions to global economic development but are plagued by challenges such as groundwater salinization. Groundwater serves as the primary source for drinking, industrial, and domestic purposes in these coastal areas. Therefore, understanding the causes and processes of groundwater salinization holds paramount significance for effective groundwater management. The coastal area of Laizhou Bay in northern China serves as a quintessential example of such a scenario. With substantial groundwater extraction and severe groundwater salinization issues, it exacerbates the disparity between water-resource supply and demand. Currently, our understanding of the processes and influencing factors related to groundwater salinization in this region remains limited. In this study, employing hydrochemical and stable chlorine isotope analyses on 35 groundwater and seawater samples, an in-depth investigation into the complex mechanisms underlying groundwater salinization in the Quaternary aquifers of the eastern coastal plain of Laizhou Bay was conducted. The test results of the samples indicate that brine and saline groundwater are primarily of the Na-Cl type, exhibiting a hydrochemical composition similar to that of seawater. Brackish groundwater exhibits a diverse hydrochemical composition. The hydrogen and oxygen isotope characteristics of brackish and fresh groundwater resemble atmospheric precipitation, while brine, seawater, and saline groundwater show hydrogen and oxygen isotope depletion. Compared to seawater, brine exhibits significant δ37Cl depletion. The analysis of the test results reveals that the formation of brine aquifers results from a complex interplay of climate change, tectonic movements, and sea–land evolution, involving lagoon development during seawater regression, salt concentration through evaporation, and subsequent water–rock interactions. The genesis of saline groundwater involves a complex interplay of brine–seawater mixing, significant evaporation, and potential input of fresh groundwater from atmospheric precipitation and river sources. The formation of brackish groundwater is predominantly influenced by atmospheric precipitation, and agricultural activities, with significant variations in NO3− concentrations attributed to varying intensities of fertilizer application in the northern plain area. These insights contribute to a deeper understanding of the origins of groundwater and can inform the development of policies for groundwater protection in this area.

Phenazine compounds are widely used in agricultural control and the medicine industry due to their high inhibitory activity against pathogens and antitumor activity. The green and sustainable method of synthesizing phenazine compounds through microbial fermentation often requires a complex culture medium containing tryptone and yeast extract, and its cost is relatively high, which greatly limits the large-scale industrial production of phenazine compounds by fermentation. The aim of this study was to develop a cost-effective minimal medium for the efficient synthesis of phenazine compounds by Pseudomonas chlororaphis. Through testing the minimum medium commonly used by Pseudomonas, an ME medium for P. chlororaphis with a high production of phenazine compounds was obtained. Then, the components of the ME medium and the other medium were compared and replaced to verify the beneficial promoting effect of Fe2+ and NH4+ on phenazine compounds. A cost-effective general defined medium (GDM) using glycerol as the sole carbon source was obtained by optimizing the composition of the ME medium. Using the GDM, the production of phenazine compounds by P. chlororaphis reached 1073.5 mg/L, which was 1.3 times that achieved using a complex medium, while the cost of the GDM was only 10% that of a complex medium (e.g., the KB medium). Finally, by engineering the glycerol metabolic pathway, the titer of phenazine-1-carboxylic acid reached the highest level achieved using a minimum medium so far. This work demonstrates how we systematically analyzed and optimized the composition of the medium and integrated a metabolic engineering method to obtain the most cost-effective fermentation strategy.

This paper employs an innovative investigation approach to study pore evolution in Al-Si-Mg-Cu alloy within aluminum foam sandwiches (AFS) by integrating data from heating–expansion ratio curves, in situ observation of synchronous radiation, and microscopic analysis of the matrix’s microstructure at different stages. Additionally, the cavity design and plate type control for large-scale AFS production are explored. Findings categorize the precursor heating into three stages: rapid heating, solid–liquid transition, and stable foaming. During solid–liquid transition, the expansion rate experiences a sudden drop, associated with pore nucleation and edge cracking of precursors. Pores nucleate as elongated crack-like structures along the rolling direction, guided by the Mg-enriched regions. In stable foaming, these pores evolve, become spherical, and the matrix rapidly expands. Using square tubes for sealing on the preform cavity sides creates a dense edge zone during rolling, halting crack propagation into the powder core. Adopting edge sealing during foaming mitigates boundary effects, thereby improving AFS panel flatness.

Continuous-variable quantum key distribution (CV-QKD) is a promising protocol that can be easily integrated with classical optical communication systems. However, in the case of quantum-classical co-transmissions, such as dense wavelength division multiplexing with classical channels and time division multiplexing with large-power classical signal, a quantum signal is more susceptible to crosstalk caused by a classical signal, leading to signal distortion and key distribution performance reduction. To address this issue, we propose a noise-suppression scheme based on carrier frequency switching (CFS) that can effectively mitigate the influence of large-power random noise on the weak coherent state. In this noise-suppression scheme, a minimum-value window of the channel’s noise power spectrum is searched for and the transmission signal frequency spectrum shifts to the corresponding frequency to avoid large-power channel noise. A digital filter is also utilized to filter out most of the channel noise. Simulation results show that compared to the traditional fixed carrier frequency scheme, the proposed noise-suppression scheme can reduce the excess noise to 1.8%, and the secret key rate can be increased by 1.43 to 2.86 times at different distances. This noise-suppression scheme is expected to be applied in scenarios like quantum–classical co-transmission and multi-QKD co-transmission to provide noise-suppression solutions.

Landslides are caused by rainfall as one of the main factors. In order to study the effect of rainfall on the physical and mechanical parameters of landslides, a physical model of the colluvium landslide is created in laboratory conditions with silty clay, river sand, and gravel, taking Shuping landslide in the Three Gorges Reservoir area as the prototype. The artificial rainfall is applied to the accumulation model, which is steady for 60 h, and then the gravel soil is taken out along the different elevations of the colluvium for the permeability test and direct shear test, and the evolution law of changes in porosity, the permeability coefficient, and the shear strength parameters along the elevation are studied. Combined with XRF and NMR tests, the spatial variation of the permeability coefficient and shear strength parameters is discussed from the perspective of chemical elements, minerals content, and porosity, and the stability analysis of a colluvium landslide is carried out considering the influence of parameters along the elevation. The results show that under the action of rainfall seepage, the fine particles of clay are transported from upslope to downslope, resulting in more and more fine particles of clay at the toe slope. The original pores are gradually filled, the cementation between particles is stronger, the corresponding cohesion is increased, and the permeability coefficient is reduced. Due to the loss of fine particles at the upslope, the relative content of coarse particles increases, leading to an increase in the internal friction angle. The variability of the slope’s physical and mechanical parameters is a result of the spatial transport of clay particles in the colluvium caused by the rainfall seepage above. Specifically, the permeability coefficient and internal friction angle from upslope to downslope decrease linearly under the action of rainfall, but the law of the cohesion increases linearly. The upslope’s permeability coefficient and internal friction angle decrease by 11% and 8% compared to those of the downslope, while the cohesion increases by 168%. The results of FLAC3D numerical calculation of Shuping landslide show that the maximum deformation in the X direction of 145 m and 175 m water level increases by 12% and 42%, and the safety factor decreases by 0.63% and 5% under the combined action of rainfall and the reservoir water level, that is, when considering the variation of parameters along the elevation of the landslide. The research findings provide a better understanding of the spatial parameters in similar colluvium bodies under rainfall action.

There is growing evidence that long-term exposure to prometryn (a widely used herbicide) can induce toxicity in bony fish and shrimp. Our previous study demonstrated its 96 h acute toxicity on the crab Eriocheir sinensis. However, studies on whether longer exposure to prometryn with a lower dose induces toxicity in E. sinensis are scarce. Therefore, we conducted a 20 d exposure experiment to investigate its effects on the hepatopancreas and intestine of E. sinensi. Prometryn reduce the activities of antioxidant enzymes, increase the level of lipid peroxidation and cause oxidative stress. Moreover, long-term exposure resulted in immune and detoxification fatigue, while short-term exposure to prometryn could upregulate the expression of genes related to immunity, inflammation and detoxification. Prometryn altered the morphological structure of the hepatopancreas (swollen lumen) and intestine (shorter intestinal villi, thinner muscle layer and thicker peritrophic membrane). In addition, prometryn changed the species composition of the intestinal flora. In particular, Bacteroidota and Proteobacteria showed a dose-dependent decrease accompanied by a dose-dependent increase in Firmicutes at the phylum level. At the genus level, all exposure groups significantly increased the abundance of Zoogloea and a Firmicutes bacterium ZOR0006, but decreased Shewanella abundance. Interestingly, Pearson correlation analysis indicated a potential association between differential flora and hepatopancreatic disorder. Phenotypic abundance analysis indicated that changes in the gut flora decreased the intestinal organ’s resistance to stress and increased the potential for opportunistic infection. In summary, our research provides new insights into the prevention and defense strategies in response to external adverse environments and contributes to the sustainable development of E. sinensis culture.

The fully mechanized caving roadway’s floor heave has a significant impact on the stability of the narrow coal pillars, the filling body next to the roadway, as well as the entire roadway. Significant floor heave necessitates extensive maintenance and rebuilding work, which has a negative impact on the mine’s regular operations. The costs of sustaining and maintaining the roads are significantly increased by production. In this study, a mechanical model of the floor heave of the road along the goaf is established using the Winker elastic foundation theory model. The mechanical model of the floor heave of the roadway is confirmed when combined with engineering cases. The findings of the study indicate that there is almost no deformation of the side floor of the roadside support and the solid coal. The floor deformation of the roadway area exhibits non-positive symmetry and a “parabolic” characteristic. Roadway width, burial depth, and roadway floor heave all have linearly positive correlations, but elastic modulus of the floor, burial depth, and highway floor heave all have negatively exponential correlations. The maximum deformation of the floor heave, which has a maximum value of 628 mm, is close to the side of the roadway support body; the theoretical model’s maximum value for the floor heave after 100 days of actual deformation monitoring is 645 mm. Between the maximum value and the maximum value as measured, there is a 2.6% error. The paper has important guiding significance for explaining the mechanism of floor heave in goaf roadway and controlling the deformation of the roadway floor.

Ulcerative colitis (UC) is an inflammatory bowel disease (IBD), and its pathogenesis is related to intestinal mucosal barrier damage and gut microbiota imbalance. Protopine (PRO), an isoquinoline alkaloid, is one of the main anti-inflammatory ingredients of traditional Chinese medicine Macleaya cordata (Willd.) R. Br. This study investigated the effects of PRO on the intestinal mucosal barrier and gut microbiota in dextran sodium sulfate (DSS)-induced colitis mice. C57BL/6J mice were treated with 3% DSS in drinking water to induce acute colitis, while PRO was administered orally once daily for 7 days. The results showed that PRO administration significantly alleviated the symptoms of DSS-induced colitis in mice and inhibited the expression of inflammation-related genes. In addition, PRO restored the integrity of the intestinal barrier in colitis mice by restoring colonic mucin secretion and promoting the expression of tight junction proteins. Furthermore, PRO alleviated the DSS-induced gut microbiota dysbiosis by decreasing the abundance of Proteobacteria, Escherichia-Shigella and Enterococcus, as well as enhancing the abundance of beneficial bacteria, such as Firmicutes and Akkermansia. These findings suggested that PRO effectively alleviated DSS-induced ulcerative colitis by suppressing the expression of inflammation-related genes, maintaining the intestinal mucosal barrier and regulating the intestinal microbiota.

For persistent organic pollutants, a concern of environmental supervision, predicted no-effect concentrations (PNECs) are often used in ecological risk assessment, which is commonly derived from the hazardous concentration of 5% (HC5) of the species sensitivity distribution (SSD). To address the problem of a lack of toxicity data, the objectives of this study are to propose and apply two improvement ideas for SSD application, taking polycyclic aromatic hydrocarbons (PAHs) as an example: whether the chronic PNEC can be derived from the acute SSD curve; whether the PNEC may be calculated by HC10 to avoid solely statistical extrapolation. In this study, the acute SSD curves for eight PAHs and the chronic SSD curves for three PAHs were constructed. The quantity relationship of HC5s between the acute and chronic SSD curves was explored, and the value of the assessment factor when using HC10 to calculate PNEC was derived. The results showed that, for PAHs, the chronic PNEC can be estimated by multiplying the acute PNEC by 0.1, and the value of the assessment factor corresponding to HC10 is 10. For acenaphthene, anthracene, benzo[a]pyrene, fluoranthene, fluorene, naphthalene, phenanthrene, and pyrene, the chronic PNECs based on the acute HC10s were 0.8120, 0.008925, 0.005202, 0.07602, 2.328, 12.75, 0.5731, and 0.05360 μg/L, respectively.

To investigate the potential of H2-calponin (CNN2) as a serum biomarker for hepatocellular carcinoma (HCC), this study employed the serological analysis of recombinantly expressed cDNA clone (SEREX) technique to identify the presence of CNN2 antibody in the serum of patients with HCC and other tumors. The CNN2 protein was produced through genetic engineering and used as an antigen to determine the positive rate of serum CNN2 autoantibodies via indirect enzyme-linked immunosorbent assay (ELISA). In addition, the mRNA and protein expressions of CNN2 in cells and tissues were evaluated using RT-PCR, in situ RT-PCR, and immunohistochemistry methods. The HCC group exhibited a significantly higher positive rate of anti-CNN2 antibody (54.8%) compared to gastric cancer (6.5%), lung cancer (3.2%), rectal cancer (9.7%), hepatitis (3.2%), liver cirrhosis (3.2%), and normal tissues (3.1%). The positive rates of CNN2 mRNA in HCC with metastasis, non-metastatic HCC, lung cancer, gastric cancer, nasopharyngeal cancer, liver cirrhosis, and hepatitis were 56.67%, 41.67%, 17.5%, 10.0%, 20.0%, 53.13%, and 41.67%, respectively. Meanwhile, the positive rates of CNN2 protein were 63.33%, 37.5%, 17.5%, 27.5%, 45%, 31.25%, and 20.83%, respectively. The down-regulation of CNN2 could inhibit the migration and invasion of liver cancer cells. CNN2 is a newly identified HCC-associated antigen that is implicated in the migration and invasion of liver cancer cells, making it a promising target for liver cancer therapy.

The rational integration of many microfluidic chips and micropumps remains challenging. Due to the integration of the control system and sensors in active micropumps, they have unique advantages over passive micropumps when integrated into microfluidic chips. An active phase-change micropump based on complementary metal–oxide–semiconductor–microelectromechanical system (CMOS-MEMS) technology was fabricated and studied theoretically and experimentally. The micropump structure is simple and consists of a microchannel, a series of heater elements along the microchannel, an on-chip control system, and sensors. A simplified model was established to analyze the pumping effect of the traveling phase transition in the microchannel. The relationship between pumping conditions and flow rate was examined. Based on the experimental results, the maximum flow rate of the active phase-change micropump at room temperature is 22 µL/min, and long-term stable operation can be achieved by optimizing heating conditions.

Laser ranging systems are a widely used form of electronic system and are generally influenced by complex electromagnetic environments. We used a laser ranging system comprising the VL53L0X laser ranging sensor module as the research object and designed a sensitive experimental platform based on the laser ranging system. In this work, we have shown that electromagnetic interference signals can affect the performance of laser ranging systems. The electromagnetic interference signal was found to enter through the chip selection port of the laser sensor chip to affect the work of the laser sensor chip. Finally, we obtained the susceptibility threshold characteristics of the laser ranging system under the influence of different types of interference signal. This study deepens the understanding of the electromagnetic susceptibility mechanism of laser ranging systems and helps in the design of their electromagnetic compatibility.

During the early seedling stage, rice (Oryza sativa L.) must overcome low-temperature stress. While a few cold-tolerance genes have been characterized, further excavation of cold-resistance genes is still needed. In this study, we identified a cold-induced transcription factor—LUX ARRHYTHMO (LUX)—in rice. OsLUX was found to be specifically expressed in leaf blades and upregulated by both cold stress and circadian rhythm. The full-length OsLUX showed autoactivation activity, and the OsLUX protein localized throughout the entire onion cell. Overexpressing OsLUX resulted in increased cold tolerance and reduced ion leakage under cold-stress conditions during the seedling stage. In contrast, the knockout of OsLUX decreased seedling cold tolerance and showed higher ion leakage compared to the wild type. Furthermore, overexpressing OsLUX upregulated the expression levels of oxidative stress-responsive genes, which improved reactive oxygen species (ROS) scavenging ability and enhanced tolerance to chilling stress. Promoter analysis showed that the OsLUX promoter contains two dehydration-responsive element binding (DREB) motifs at positions −510/−505 (GTCGGa) and −162/−170 (cCACCGccc), which indicated that OsDREB1s and OsDREB2s probably regulate OsLUX expression by binding to the motif to respond to cold stress. Thus, OsLUX may act as a downstream gene of the DREB pathway. These results demonstrate that OsLUX serves as a positive regulatory factor of cold stress and that overexpressing OsLUX could be used in rice breeding programs to enhance abiotic stress tolerance.

Ultrafine Polyvinyl alcohol (PVA) fibers have an outstanding potential in various applications, especially in absorbing fields. In this manuscript, an electrostatic-field-assisted centrifugal spinning system was designed to improve the production efficiency of ultrafine PVA fibers from PVA aqueous solution for NH3 adsorption. It was established that the fiber production efficiency using this self-designed system could be about 1000 times higher over traditional electrospinning system. The produced PVA fibers establish high morphology homogeneity. The impact of processing variables of the constructed spinning system including rotation speed, needle size, liquid feeding rate, and voltage on fiber morphology and diameter was systematically investigated by SEM studies. To acquire homogeneous ultrafine PVA fiber membranes, the orthogonal experiment was also conducted to optimize the spinning process parameters. The impact weight of different studied parameters on the spinning performance was thus provided. The experimental results showed that the morphology of micro/nano-fibers can be well controlled by adjusting the spinning process parameters. Ultrafine PVA fibers with the diameter of 2.55 μm were successfully obtained applying the parameters, including rotation speed (6500 rpm), needle size (0.51 mm), feeding rate (3000 mL h−1), and voltage (20 kV). Furthermore, the obtained ultrafine PVA fiber mat was demonstrated to be capable of selectively adsorbing NH3 gas relative to CO2, thus making it promising for NH3 storage and other environmental purification applications.

The isolation of single cells is essential for the development of single cell analysis methods, such as single-cell sequencing, monoclonal antibodies, and drug development. Traditional single-cell isolation techniques include flow cytometry (FACS), laser capture microdissection (LCM), micromanipulation, etc., but their operations are complex and have low throughput. Here, we present a microfluidic chip that can isolate individual cells from cell suspension and release them onto a well plate. It uses thermal bubble micropump technology to drive the fluid flow, and single-cell isolation is achieved by matching the flow resistance of the flow channel. Therefore, injection pumps and peristaltic pumps are not required for cell loading. Because of its small size, we can integrate hundreds of single-cell functional modules, which makes high-throughput single-cell isolation possible. For polystyrene beads, the capture rate of the single bead is close to 100%. Finally, the method has been applied to cells, and the capture rate of the single cell is also about 75%. This is a promising method for single-cell isolation.

The impact resistance of aluminum foam sandwich panels (AFS) with metallurgical bonding interfaces prepared by the powder cladding rolling method was investigated. Low-velocity impact tests were conducted by using a drop-weight impact facility to explore the dynamic mechanical behavior, deformation and damage mechanisms, and energy absorption of AFS with metallurgical bonding interfaces. The effects of variation of impact energy, panel thickness, and specimen density on the energy absorption performance of AFS were quantitatively evaluated by energy absorption indicators. The results indicate that the load-displacement curve illustrates prominent three-stage characteristics when the impact energy is 120 J containing the front panel yielding stage, the foam core’s compressive and shear failure stage, and the back panel fracture stage. The impact strength of the sandwich structure increases with increasing panel thickness and specimen density. The AFS with metallurgical bonding interfaces presents favorable energy absorption efficiency under low velocity.

As underwater disturbances (natural or artificial) occur in the ocean, moving seawater crossing the geomagnetic fields will produce weak circular currents. These currents can induce measurable magnetic fields, which might be useful for monitoring ocean internal waves using aeromagnetic survey. In this research, a spectral-element method (SEM) based on Gauss–Lobatto–Legendre (GLL) polynomials is presented to characterize the magnetic field induced by the underwater pressure waves. A concise mathematical model is established through combining the acoustic wave equations and Maxwell’s equations. Specifically, the acoustic–magnetic coupling simulation adopts the nodal-based SEM for acoustic analysis and edge-based SEM for electromagnetic analysis. The proposed SEM has spectral accuracy, as the error exponentially decreases with the order of the basis functions. Additionally, by adopting an independent modeling and mesh scheme in two solvers, respectively, the waste of computing resources is avoided. The experimental analysis demonstrates that the induced magnetic fields mechanically propagate with the acoustic wave, producing the pseudo-radiation phenomenon. The signals of these magnetic fields may extend for tens of kilometers and exist for hours under certain circumstances, which provide a theoretical basis for underwater target identification via high-sensitivity atomic magnetometer.

Oxidative stress is recognized as a significant contributor to the development and progression of inflammation and disruptions in the balance of gut microflora, commonly referred to as intestinal dysbiosis. It is crucial that safe and effective antioxidant and anti-inflammatory agents are identified to address these conditions. Ampelopsis grossedentata, a natural plant abundant in flavonoids and primarily found in southern China, has demonstrated potent antioxidant properties. However, the extent to which flavonoids in A. grossedentata impact intestinal inflammation and alter the composition of the gut microbiome remains to be fully understood. The purpose of this study was to explore the potential benefits of using A. grossedentata as an antioxidant and anti-inflammatory agent in the context of intestinal inflammation, both in vitro and in vivo. We first conducted an initial comparison of the effects of dihydromyricetin (DMY), an alcohol extract of A. grossedentata (AEA, 82% total flavonoids), and a water extract of A. grossedentata (WEA, 57% total flavonoids) on the cell viability and intestinal barrier integrity of porcine epithelial cells IPEC-J2. Although the total flavonoid content is much lower in WEA than in AEA, the results show that they have similar effects. Subsequently, the antioxidant properties of WEA were compared with those of commonly utilized antioxidants in vitro. Lastly, the antioxidant and anti-inflammatory properties of WEA, as well as its impacts on gut microbiota, were evaluated in animal models, including mice and Drosophila. In summary, the results of our study indicate that WEA, due to its antioxidant properties, exhibits a protective effect on the intestinal barrier function in porcine epithelial cell line IPEC-J2. Additionally, WEA demonstrates a positive correlation with DPPH, ABTS radical scavenging rate, FRAP, and reducing power under in vitro settings. Furthermore, WEA was shown to effectively alleviate oxidative stress in animal models by reducing the levels of pro-inflammatory cytokines and increasing the antioxidant enzyme activity in the liver, as well as by activating the Nrf2 signaling pathway in the duodenum. Additionally, WEA was able to regulate gut microbiota, promoting the growth of beneficial bacteria and inhibiting harmful microbes, as well as extending the lifespan of Drosophila. Overall, these findings suggest that WEA may serve as a valuable dietary supplement for addressing oxidative stress and inflammation through its anti-inflammatory and prebiotic effects, which are conferred via the Nrf2/Keap1 pathway.

The quality of blueberry fruit is easily altered after harvest. We investigated the regulatory mechanism of heat-shock (postharvest treatment) and edible coating (preharvest treatment) on the post-harvest physiological quality of blueberry from the perspective of physiological, biochemical and organoleptic characteristics. In our research, the optimal TKL concentration and the appropriate range of heat-shock temperatures were first screened based on actual application results, and then a combination of heat-shock temperature and TKL coating with significant differences in preservation effects was selected to investigate the effects of different heat-shock temperatures and TKL60 composite coating on post-harvest quality and volatile compound concentration of blueberries under refrigerated conditions. Our results showed that TKL with 60 mg/L thymol can retard the development of the degree of membrane lipid peroxidation and effectively reduce the incidence of fruit decay and the severity of blueberries infected with major pathogens at 25 °C. Meanwhile, heat-shock treatments were effective in maintaining the quality of blueberries, with a certain advantage from 45 °C to 65 °C after 8 d of storage at ambient temperature, but these treated groups were slightly inferior to TKL60 groups for fresh-keeping effect. Remarkably, the combination of heat-shock treatment and edible coating application could extend the shelf life of blueberries by 7–14 d compared to the results obtained with coating alone under low temperature storage. Specifically, heat treatment at 45 °C for 60 min after TKL60 coating (HT2) retarded the decrease in the levels of ascorbic acid, total anthocyanin, total acid and soluble solids. Gas chromatography–mass spectrometry hierarchical clustering analysis showed that this treatment also improved the aroma of the fruit, which maintained a certain similarity with that of fresh blueberries after 14 d. Principal component analysis (PCA) of the results of the evaluations carried out using an electronic nose (E-nose) and electronic tongue (E-tongue) showed that blueberries of the HT2 treated group did not show a large placement change of the PC1 distribution area from that of the fresh and blank control group. Accordingly, the combination of coating with heat-shock treatment can effectively improve the post-harvest quality and aroma compound concentration of blueberries, showing good application potential in storage and preservation of fresh fruits such as blueberries.

The effect of porosity and pore size on the quasi-static compression properties and energy absorption characteristics of the steel foam was investigated in this paper. The 316L steel foams were prepared through powder metallurgy using urea as the space holder. The macrostructure of steel foam and microstructure of the pore walls were characterized, and the quasi-static compression experiments were conducted on the specimens in the axial direction at a strain rate of 10−3 s−1. The results show that the increase in porosity decreases the yield strength and plastic modulus of the steel foam but increases the densification strain of the steel foam. The yield strength of the steel foam decreases significantly when the pore size is 2.37 mm. However, the pore size has little effect on the plastic modulus. Moreover, the energy absorption per volume of the steel foam decreases with increasing porosity at the same strain. The effect of porosity on energy absorption efficiency is greater than that of pore size.

A large amount of fluorine−containing wastewater was produced with the rapid development of the industry. Excessive fluoride content in water will not only endanger ecological security but also pose threat to human health. In this study, common new adsorbents for defluorination, such as metal−based adsorbents, natural adsorbents, and nanomaterial adsorbents were reviewed for its physicochemical properties and fluorine removal efficiency. The fluorine removal mechanism of different adsorbents was introduced in detail, and the future work of the removal of fluorine using novel adsorbents was proposed. This research also introduces the application of the coupling of the adsorption method with the technology of chemical precipitation, filtration, and super−magnetic separation to treat high concentration of fluoride wastewater. A good selection of process combinations according to different needs can achieve high−efficiency defluorination in water. Finally, some existing problems of practical operation of fluorine using removal materials in the environment are summarized, hoping to contribute to the future research of fluorine removal materials.

Mitochondrial isocitrate dehydrogenase 2 (IDH2) is an important metabolic enzyme in the tricarboxylic acid cycle (TCA) cycle. Our previous study showed that high expression of wild-type IDH2 promotes the proliferation of lung cancer cells. This study aims to test the potential of targeting IDH2 as a therapeutic strategy to inhibit lung cancer in vitro and in vivo. First, we analyzed the available data from the databases gene expression omnibus (GEO) database to evaluate the clinical relevance of IDH2 expression in affecting lung cancer patient survival. We then generated a stable IDH2-knockdown lung cancer cell line using a lentivirus-based method for in vitro and in vivo study. Cell growth, apoptosis, cell viability, and colony formation assays were conducted to test the sensitivity of lung cancer cells with different IDH2 expression status to cisplatin or radiation treatment in vitro. For mechanistic study, Cellular oxygen consumption and extracellular acidification rates were measured using a Seahorse metabolic analyzer, and reactive oxygen species (ROS) generation was analyzed using flow cytometry. An animal study using a xenograft tumor model was performed to further evaluate the in vivo therapeutic effect on tumor growth. We found that high IDH2 expression was associated with poor survival in lung cancer patients undergoing chemotherapy. Inhibition of IDH2 significantly enhanced the anticancer activity of cisplatin and also increased the effect of radiation against lung cancer cells. IDH2 was upregulated in cisplatin-resistant lung cancer cells, which could be sensitized by targeted inhibition of IDH2. Mechanistic study showed that abrogation of IDH2 caused only minimal changes in oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in lung cancer cells, but induced a significant increase in ROS, which rendered the cancer cells more sensitive to cisplatin. Pretreatment of lung cancer cells with the ROS scavenger N-acetyl-cysteine could partially rescue cells from the cytotoxic effect of cisplatin and IDH2 inhibition. Importantly, abrogation of IDH2 significantly increased the sensitivity of lung cancer cells to cisplatin in vivo.

In this paper, an aluminum foam sandwich (AFS) was prepared by the rolling composite-powder metallurgy method, and its fatigue properties were studied. It was compared with an AFS made by the adhesive method to study its fatigue properties more directly. In this experiment, the fatigue performance was investigated by studying the microscopic interface, fatigue life, deflection curve, and failure mode. The results show that the fatigue life of an AFS with the rolling composite-powder metallurgy method is much longer than that with the adhesive method. The failure mode of an AFS made by the rolling composite-powder metallurgy method is shear failure, and that of an AFS made by the adhesive method is shear failure and interface debonding. An AFS with the rolling composite-powder metallurgy method has better fatigue properties. This paper also explored the fatigue damage model using the fatigue modulus method, and the polynomial fitting method has a higher fitting degree.

Shaziling pigs, as a native Chinese breed, have been classified as a fatty liver model. As the core of the whole pig farm, the sow’s organism health is especially important, especially in the perinatal period; however, there are few reports on the perinatal intestinal microbiology and bile acid metabolism of Shaziling pig sows. The purpose of this research was to investigate the alterations in bile acids and gut microbiota of sows that occur throughout the perinatal period. Forty-two sows were selected for their uniformity of body conditions and were given the same diet. Fecal samples were collected for 16srDNA sequencing and bile acid targeted metabolome detection in four stages (3 days before delivery, 3 days after delivery, 7 days after delivery and 21 days after delivery). As revealed by the results, there were statistically significant variations in bile acids among the four stages, with the concentration of bile acids identified by SZL-4 being substantially greater than that of the other three groups (p < 0.05). When compared to the other three groups (p < 0.05), SZL-2 had considerably lower Shannon, Simpson and Chao 1 indices, and exhibited a statistically significant difference in β-diversity. SZL-2 samples included a greater proportion of Proteobacteria than SZL-3 and SZL-4 samples; however, SZL-2 samples contained a smaller proportion of spirochetes than SZL-3 and SZL-4 samples. To a large extent, lactic acid bacteria predominated in the SZL-2 samples. The LEfSe analysis showed that the relative abundances of Lachnospiraceae_XPB1014_group, Christensenellaceae_R_7_group, Clostridium, Collinsella, Turicibacter, and Mollicutes_RF39_unclassified were the main differential bacteria in the SZL-1 swine fecal samples and the Eubacterium__coprostanoligenes_group in sow fecal samples from SZL-2. The relative abundance of Bacteroides, UBA1819, Enterococcus, Erysipelatoclostridium, and Butyricimonas in SZL-3 and SZL-4 Streptococcus, Coriobacteriaceae_unclassified, Prevotellaceae_UCG_001, Streptomyces, and Ochrobactrum in SZL-3. g_Collinsella was significantly and positively correlated with vast majority bile acids, and the g_Lachnospiraceae_XPB1014_group with GCDCA and GHDCA into positive correlations. Simultaneously, g_Streptococcus, g_Bacteroides, and g_UBA1819 inversely correlated with bile acid, accounting for the great bulk of the difference. In conclusion, there is an evident correlation between bile acids and gut microbiota in the perinatal period of Shaziling sows. Additionally, the discovery of distinct bacteria associated to lipid metabolism gives a reference for ameliorating perinatal body lipid metabolism disorder of sows through gut microbiota.

Coal gangue, as a solid waste produced in the coal mining process, can be disposed by being prepared into backfill materials and then filled in underground goafs, thus controlling strata movement and surface subsidence. However, gangue backfill materials are non-continuous; therefore, research into the surface deformation induced by backfill mining should consider the creep compression behavior of gangue backfill materials. The research took a backfill panel in Tangshan Coal Mine (Tangshan City, Hebei Province, China) as the background. In addition, broken coal gangue was collected in the field to prepare specimens of gangue backfill materials, and their creep compression properties were measured. The corresponding constitutive equation of creep compression was then established and embedded in the numerical software, FLAC3D. By building the numerical model for surface deformation induced by backfill mining, the surface deformation above the backfill panel under conditions of different creep durations of backfill materials was simulated and evaluated. In addition, two measuring lines were arranged on the surface to monitor changes in surface subsidence. After surface subsidence stabilized, the maximum surface subsidence was 163.4 mm, which satisfied the fortification criterion of surface buildings. This means the backfill mining did not affect nearby buildings. The results provide a theoretical basis for predicting surface deformation induced by backfill mining and its effective control.

Phytochrome-interacting factors (PIFs) are transcription factors with the basic helix–loop–helix (bHLH) domain. As integration factors between different signal pathways, members of the PIF protein family regulate many aspects of plant growth and development, such as seed germination, photomorphogenesis, thermomorphogenesis, rhythm regulation, flowering response, stomatal development, and stress responses. Our previous studies have shown that the BpSPL2 gene may regulate plants’ adventitious root development through PIF genes. Within the Betula platyphylla genome, we identified eight PIF (BpPIFs) genes. We analysed and named them based on a phylogenetic tree, gene structures, and conserved motifs. Synteny analysis indicated that transposition or segmental duplication events played a minor role in the expansion of BpPIFs. The comparative syntenic analysis combined with phylogenetic analysis provided a deep insight into the phylogenetic relationships of BpPIF genes, suggesting that BpPIF proteins are closer to PtPIF than to AtPIF. The analysis of cis-acting elements in promoter regions of BpPIF genes indicated that various elements were related to light, abiotic stress, and plant hormone responsiveness. In addition, we found that these promoters have the transcription factor of B. platyphylla SPL2 (BpSPL2) binding motif GTAC. Expression analysis demonstrated that BpPIF genes, especially BpPIF4, BpPIF9b, and BpPIF10, might be the potential target genes of BpSPL2 in the process of adventitious root formation. Besides providing a comprehensive understanding of the BpPIF family, we propose a hypothetical gene network regulatory model for adventitious root formation.

In field harvesting conditions, the non-stationary random vibration characteristics of the harvester are rarely considered, and the results of vibration frequency calculated by different time–frequency transformation methods are different. In this paper, the harvester’s vibration characteristics under the time-varying mass were studied, and the correlation between vibration frequency and modal frequency was analyzed. Firstly, under the conditions of time-varying mass (field harvesting conditions) and non-time-varying mass (empty running condition), the non-stationarity characteristics of vibration signals at 16 measurement points of a combined corn harvester frame were studied. Then, fast Fourier transform (FFT), short-time Fourier transform (STFT), and continuous wavelet transform (CWT) were used to calculate the vibration frequency distribution characteristics of the corn harvester. Finally, based on the EFDD (enhanced frequency domain decomposition) algorithm, the correlation between the primary vibration frequency and the operating mode frequency is studied. The results show that the mean, variance, and maximum difference of the vibration amplitude under harvesting conditions (mass time-varying system) are 0.10, 26.5, and 1.0, respectively, at different harvesting periods (0~10 s, 10~20 s, 20~30 s). The harvesting conditions’ vibration signals conform to the characteristics of non-stationary randomness. The FFT algorithm is used to obtain more dense vibration frequencies, while the frequencies based on STFT and CWT algorithms are sparse. The correlation between the FFT method and the EFDD algorithm is 0.98, and the correlation between the STFT, CWT, and the EFDD algorithm is 0.99 and 0.98. Therefore, the primary frequency of the STFT methods is closer to the modal frequency. Our research laid the foundation for further study and application of mass time-varying combined harvester system non-stationary random vibration modal frequency identification and vibration control.

The skin is the largest organ of the body and plays multiple essential roles, ranging from regulating temperature, preventing infections, to ultimately affecting human health. A hair follicle is a complex cutaneous appendage. Skin diseases and hair loss have a significant effect on the quality of life and psychosocial adjustment of individuals. However, the available traditional drugs for treating skin and hair diseases may have some insufficiencies; therefore, a growing number of researchers are interested in natural materials that could achieve satisfactory results and minimize adverse effects. Natural polyphenols, named for the multiple phenolic hydroxyl groups in their structures, are promising candidates and continue to be of scientific interest due to their multifunctional biological properties and safety. Polyphenols have a wide range of pharmacological effects. In addition to the most common effect, antioxidation, polyphenols have anti-inflammatory, bacteriostatic, antitumor, and other biological effects associated with reduced risk of a number of chronic diseases. Various polyphenols have also shown efficacy against different types of skin and hair diseases, both in vitro and in vivo, via different mechanisms. Thus, this paper reviews the research progress in natural polyphenols for the protection of skin and hair health, especially focusing on their potential therapeutic mechanisms against skin and hair disorders. A deep understanding of natural polyphenols provides a new perspective for the safe treatment of skin diseases and hair loss.

Reactive oxygen species (ROS) homeostasis and mitochondrial metabolism are critical for the survival of cancer cells, including cancer stem cells (CSCs), which often cause drug resistance and cancer relapse. Auranofin is a mono-gold anti-rheumatic drug, and it has been repurposed as an anticancer agent working by the induction of both ROS increase and mitochondrial dysfunction. Hypothetically, increasing auranofin’s positive charges via incorporating more gold atoms to enhance its mitochondria-targeting capacity could enhance its anti-cancer efficacy. Hence, in this work, both mono-gold and bi-gold compounds were designed and evaluated to test our hypothesis. The results showed that bi-gold compounds generally suppressed cancer cells proliferation better than their mono-gold counterparts. The most potent compound, BGC2a, substantially inhibited the antioxidant enzyme TrxR and increased the cellular ROS. BGC2a induced cell apoptosis, which could not be reversed by the antioxidant agent vitamin C, implying that the ROS induced by TrxR inhibition might not be the decisive cause of cell death. As expected, a significant proportion of BGC2a accumulated within mitochondria, likely contributing to mitochondrial dysfunction, which was further confirmed by measuring oxygen consumption rate, mitochondrial membrane potential, and ATP production. Moreover, BGC2a inhibited colony formation and reduced stem-like side population (SP) cells of A549. Finally, the compound effectively suppressed the tumor growth of both A549 and PANC-1 xenografts. Our study showed that mitochondrial disturbance may be gold-based compounds’ major lethal factor in eradicating cancer cells, providing a new approach to developing potent gold-based anti-cancer drugs by increasing mitochondria-targeting capacity.

Oncogenic K-ras is often activated in pancreatic ductal adenocarcinoma (PDAC) due to frequent mutation (>90%), which drives multiple cellular processes, including alterations in lipid metabolism associated with a malignant phenotype. However, the role and mechanism of the altered lipid metabolism in K-ras-driven cancer remains poorly understood. In this study, using human pancreatic epithelial cells harboring inducible K-rasG12D (HPNE/K-rasG12D) and pancreatic cancer cell lines, we found that the expression of phospholipase A2 group IIA (PLA2G2A) was upregulated by oncogenic K-ras. The elevated expression of PLA2G2A was also observed in pancreatic cancer tissues and was correlated with poor survival of PDAC patients. Abrogation of PLA2G2A by siRNA or by pharmacological inhibition using tanshinone I significantly increased lipid peroxidation, reduced fatty acid synthase (FASN) expression, and impaired mitochondrial function manifested by a decrease in mitochondrial transmembrane potential and a reduction in ATP production, leading to the inhibition of cancer cell proliferation. Our study suggests that high expression of PLA2G2A induced by oncogenic K-ras promotes cancer cell survival, likely by reducing lipid peroxidation through its ability to facilitate the removal of polyunsaturated fatty acids from lipid membranes by enhancing the de novo fatty acid synthesis and energy metabolism to support cancer cell proliferation. As such, PLA2G2A might function as a downstream mediator of K-ras and could be a potential therapeutic target.

The interface bonding method has a great influence on the mechanical properties of aluminum foam sandwich (AFS). This study aims to investigate the effect of different interface bonding methods on the mechanical properties of AFS. In this paper, the metallurgical-bonding interface-formation mechanism of AFS prepared by powder metallurgy was investigated. The shear properties of metallurgical-bonded AFS were determined by the panel peeling test. The flexural properties and energy absorption of metallurgical-bonded and glued AFS were analyzed through the three-point bending test. The results show that the magnesium, silicon, and copper elements of the core layer diffuse to panels and form a metallurgical composite layer. The metallurgical-bonding strength between the panel and core layer is higher than that of the foam core layer. The peak load of metallurgically-bonded AFS is 24% more than that of glued AFS, and energy absorption is 12.2 times higher than that of glued AFS.

Taking advantage of the concurrent stretching and bending property of corrugated flexure hinges, a sinusoidal corrugated flexure linkage was proposed and applied for the construction of a corrugated dual-axial mechanism with structural symmetry and decoupled planar motion guidance. Castigliano’s second theorem was employed to derive the complete compliance for a basic sinusoidal corrugated flexure unit, and matrix-based compliance modeling was then applied to find the stiffness of the sinusoidal corrugated flexure linkage and the corrugated dual-axial mechanism. Using established analytical models, the influence of structural parameters on the stiffness of both the corrugated flexure linkage and the dual-axial mechanism were investigated, with further verification by finite element analysis, with errors less than 20% compared to the analytical results for all cases. In addition, the stiffness of the corrugated flexure mechanism was practically tested, and its deviation between practical and analytical was around 7.4%. Further, the feasibility of the mechanism was demonstrated by successfully applying it for a magnetic planar nanopositioning stage, for which both open-loop and closed-loop performances were systematically examined. The stage has a stroke around 130 μm for the two axes and a maximum cross-talk less than 2.5%, and the natural frequency is around 590 Hz.

Currently, many microchips must rely on an external force (such as syringe pump, electro-hydrodynamic pump, and peristaltic pump, etc.) to control the solution in the microchannels, which probably adds manual operating errors, affects the accuracy of fluid manipulation, and enlarges the noise of signal. In addition, the reasonable integration of micropump and microchip remain the stumbling block for the commercialization of microfluidic technique. To solve those two problems, we designed and fabricated a thermal bubble micropump based on MEMS (micro-electro-mechanical systems) technique. Many parameters (voltage, pulse time, cycle delay time, etc.) affecting the performance of this micropump were explored in this work. The experimental results showed the flow rate of solution with the assistance of a micropump reached more than 15 μL/min in the optimal condition. Finally, a method about measuring total aflatoxin in Chinese herbs was successfully developed based on the integrated platform contained competitive immunoassay and our micropump-based microfluidics. Additionally, the limit of detection in quantifying total aflatoxin (AF) was 0.0615 pg/mL in this platform. The data indicate this combined technique of biochemical assays and micropump based microchip have huge potential in automatically, rapidly, and sensitively measuring other low concentration of biochemical samples with small volume.

High cervical spinal cord stimulation (HCSCS) was found to have therapeutic effects on Parkinsonian gait disturbance. However, the results were inconsistent and confounded with symptoms of pain. This study aimed to reveal the gait and dysarthric effects of HCSCS in PD (Parkinson’s disease) and MSA-P (Parkinson variant of multiple system atrophy) patients without pain. Three PD and five MSA-P patients without painful comorbidities were assessed for gait performance and speech before SCS surgery and at 3- and 6-month follow-up. Stride length and the time spent in the Timed Up-and-Go task showed little change after HCSCS surgery. Overall voice quality (measured by dysphonia severity index) and perceptual speech intelligence improved significantly at 3 months, but improvements slightly diminished at 6 months postoperatively. Change in quality of life (measured by 8-item Parkinson’s disease questionnaire) was also notable at 3 months but narrowed over time following HCSCS. In conclusion, HCSCS showed therapeutic effects in improving the dysarthria but not gait disturbance in pain-free PD and MSA-P patients.