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1. Characterization of Reagent Pencils for Deposition of Reagents onto Paper-Based Microfluidic Devices

Author

Cheyenne H. Liu, Isabelle C. Noxon, Leah E. Cuellar, Amanda L. Thraen, Chad E. Immoos, Andres W. Martinez, and Philip J. Costanzo

Subjects
paper, diagnostics, microfluidic paper-based analytical devices (microPADs), pencil, wear, colorimetry, Mechanical engineering and machinery, TJ1-1570
Abstract

Reagent pencils allow for solvent-free deposition of reagents onto paper-based microfluidic devices. The pencils are portable, easy to use, extend the shelf-life of reagents, and offer a platform for customizing diagnostic devices at the point of care. In this work, reagent pencils were characterized by measuring the wear resistance of pencil cores made from polyethylene glycols (PEGs) with different molecular weights and incorporating various concentrations of three different reagents using a standard pin abrasion test, as well as by measuring the efficiency of reagent delivery from the pencils to the test zones of paper-based microfluidic devices using absorption spectroscopy and digital image colorimetry. The molecular weight of the PEG, concentration of the reagent, and the molecular weight of the reagent were all found to have an inverse correlation with the wear of the pencil cores, but the amount of reagent delivered to the test zone of a device correlated most strongly with the concentration of the reagent in the pencil core. Up to 49% of the total reagent deposited on a device with a pencil was released into the test zone, compared to 58% for reagents deposited from a solution. The results suggest that reagent pencils can be prepared for a variety of reagents using PEGs with molecular weights in the range of 2000 to 6000 g/mol.

Published
2017
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2. Characterization of Reagent Pencils for Deposition of Reagents onto Paper-Based Microfluidic Devices

Author

Amanda L. Thraen, Leah E. Cuellar, Isabelle C. Noxon, Philip J. Costanzo, Andres W. Martinez, Cheyenne H. Liu, and Chad E. Immoos

Subjects
wear, Absorption spectroscopy, genetic structures, lcsh:Mechanical engineering and machinery, Microfluidics, education, Analytical chemistry, microfluidic paper-based analytical devices (microPADs), 02 engineering and technology, 01 natural sciences, behavioral disciplines and activities, Article, chemistry.chemical_compound, diagnostics, lcsh:TJ1-1570, Electrical and Electronic Engineering, Inverse correlation, Chemistry, Mechanical Engineering, paper, 010401 analytical chemistry, Paper based, Polyethylene, 021001 nanoscience & nanotechnology, pencil, 0104 chemical sciences, Pencil (optics), Wear resistance, colorimetry, Chemical engineering, Control and Systems Engineering, Reagent, 0210 nano-technology
Abstract

Reagent pencils allow for solvent-free deposition of reagents onto paper-based microfluidic devices. The pencils are portable, easy to use, extend the shelf-life of reagents, and offer a platform for customizing diagnostic devices at the point of care. In this work, reagent pencils were characterized by measuring the wear resistance of pencil cores made from polyethylene glycols (PEGs) with different molecular weights and incorporating various concentrations of three different reagents using a standard pin abrasion test, as well as by measuring the efficiency of reagent delivery from the pencils to the test zones of paper-based microfluidic devices using absorption spectroscopy and digital image colorimetry. The molecular weight of the PEG, concentration of the reagent, and the molecular weight of the reagent were all found to have an inverse correlation with the wear of the pencil cores, but the amount of reagent delivered to the test zone of a device correlated most strongly with the concentration of the reagent in the pencil core. Up to 49% of the total reagent deposited on a device with a pencil was released into the test zone, compared to 58% for reagents deposited from a solution. The results suggest that reagent pencils can be prepared for a variety of reagents using PEGs with molecular weights in the range of 2000 to 6000 g/mol.

Published
2017