Back to Search
Start Over
Characterization of Reagent Pencils for Deposition of Reagents onto Paper-Based Microfluidic Devices
- Source :
- Micromachines, Vol 8, Iss 8, p 242 (2017), Micromachines, Micromachines; Volume 8; Issue 8; Pages: 242
- Publication Year :
- 2017
- Publisher :
- MDPI AG, 2017.
-
Abstract
- Reagent pencils allow for solvent-free deposition of reagents onto paper-based microfluidic devices. The pencils are portable, easy to use, extend the shelf-life of reagents, and offer a platform for customizing diagnostic devices at the point of care. In this work, reagent pencils were characterized by measuring the wear resistance of pencil cores made from polyethylene glycols (PEGs) with different molecular weights and incorporating various concentrations of three different reagents using a standard pin abrasion test, as well as by measuring the efficiency of reagent delivery from the pencils to the test zones of paper-based microfluidic devices using absorption spectroscopy and digital image colorimetry. The molecular weight of the PEG, concentration of the reagent, and the molecular weight of the reagent were all found to have an inverse correlation with the wear of the pencil cores, but the amount of reagent delivered to the test zone of a device correlated most strongly with the concentration of the reagent in the pencil core. Up to 49% of the total reagent deposited on a device with a pencil was released into the test zone, compared to 58% for reagents deposited from a solution. The results suggest that reagent pencils can be prepared for a variety of reagents using PEGs with molecular weights in the range of 2000 to 6000 g/mol.
- Subjects :
- wear
Absorption spectroscopy
genetic structures
lcsh:Mechanical engineering and machinery
Microfluidics
education
Analytical chemistry
microfluidic paper-based analytical devices (microPADs)
02 engineering and technology
01 natural sciences
behavioral disciplines and activities
Article
chemistry.chemical_compound
diagnostics
lcsh:TJ1-1570
Electrical and Electronic Engineering
Inverse correlation
Chemistry
Mechanical Engineering
paper
010401 analytical chemistry
Paper based
Polyethylene
021001 nanoscience & nanotechnology
pencil
0104 chemical sciences
Pencil (optics)
Wear resistance
colorimetry
Chemical engineering
Control and Systems Engineering
Reagent
0210 nano-technology
Subjects
Details
- Language :
- English
- Volume :
- 8
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Micromachines
- Accession number :
- edsair.doi.dedup.....b4c27bc630ade1606818933275925f45