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An event camera is a neuromimetic sensor inspired by the human retinal imaging principle, which has the advantages of high dynamic range, high temporal resolution, and low power consumption. Due to the interference of hardware and software and other factors, the event stream output from the event camera usually contains a large amount of noise, and traditional denoising algorithms cannot be applied to the event stream. To better deal with different kinds of noise and enhance the robustness of the denoising algorithm, based on the spatio-temporal distribution characteristics of effective events and noise, an event stream noise reduction and visualization algorithm is proposed. The event stream enters fine filtering after filtering the BA noise based on spatio-temporal density. The fine filtering performs time sequence analysis on the event pixels and the neighboring pixels to filter out hot noise. The proposed visualization algorithm adaptively overlaps the events of the previous frame according to the event density difference to obtain clear and coherent event frames. We conducted denoising and visualization experiments on real scenes and public datasets, respectively, and the experiments show that our algorithm is effective in filtering noise and obtaining clear and coherent event frames under different event stream densities and noise backgrounds.

During the operation of solid oxide fuel cells (SOFCs), the Ni-8YSZ anodes are subjected to thermal mismatch and reoxidation, accompanied by the risk of damage and failure. These damages and failures are generally induced by small defects at the microscopic level, leading to the degradation of the structural bearing capacity. Therefore, the distribution and quantification of the stresses in the real microstructure of Ni-8YSZ electrodes is essential. In this study, the real Ni-8YSZ microstructure was reconstructed based on nano-computed tomography, and the stress distribution of the real microstructure was analyzed based on the finite element method under reoxidation and different operating temperatures. The failure probability of 8YSZ at different degrees of reoxidation was evaluated according to the Weibull method, and the amount of damaged 8YSZ elements was statistically counted. The study results indicate a high level of stress in the thin necks and relatively sharp areas of the microstructure. The 8YSZ has a high failure probability at a reoxidation extent of 5-10%.

Bovine viral diarrhea virus (BVDV) is an RNA virus associated with severe economic losses in animal production. Effective vaccination and viral surveillance are urgent for the prevention and control of BVDV infection. However, the application of traditional modified live vaccines and inactivated vaccines is faced with tremendous challenges. In the present study, we describe the preclinical efficacy of two BVDV mRNA vaccines tested in mice and guinea pigs, followed by a field trial in goats, where they were compared to a commercial vaccine (formaldehyde inactivated). The two mRNAs were engineered to express the envelope protein E2 of BVDV-1, the most prevalent subtype across the world, through a 5' cap-dependent or independent fashion. Better titers of neutralizing antibodies against BVDV-1 were achieved using the capped RNA in the sera of mice and guinea pigs, with maximum values reaching 9.4 and 13.7 (by -log 2 ), respectively, on the 35th day post-vaccination. At the same time point, the antibody levels in goats were 9.1 and 10.2 for the capped and capless RNAs, respectively, and there were no significant differences compared to the commercial vaccine. The animals remained healthy throughout the experiment, as reflected by their normal leukogram profiles. Collectively, our findings demonstrate that mRNA vaccines have good safety and immunogenicity, and we laid a strong foundation for the further exploitation of efficient and safe BVDV vaccines.

Cytochrome P450 monooxygenases ( CYP450s ) play a variety of physiological roles, including pesticide resistance, plant allelochemical detoxification, and hormone metabolism catalysis. However, limited information is available on the classification and expression profiles of the CYP450 gene family in aphid species. This is the first study to identify the cytochrome P450 gene family in 19 aphid species at the whole genome level. A total of 1100 CYP450 genes were identified in 19 aphid species. Three hundred CYP450 genes belonged to six cereal crop aphid species, which were further classified into four subfamilies according to the phylogenetic relationship. The conserved motifs, exon-intron structures, and genomic organization of the same subfamilies were similar. Predictions of subcellular localization revealed that the endoplasmic reticulum harbored the majority of CYP450 proteins. In Sitobion avenae and Rhopalosiphum maidis , the increase in the CYP450 gene was primarily caused by segmental duplication events. However, only tandem duplication occurred in the CYP450 gene family of Diuraphis noxia , Rhopalosiphum padi , Schizaphis graminum, and Sitobion miscanthi . Synteny analysis found three continuous colinear CYP450 gene pairs among six cereal crop aphid species. Furthermore, we obtained the expression profiles of four cereal crop aphids, including R. padi , D. noxia , S. graminum, and S. avenae . Differential expression analysis provided growth stage specificity genes, tissue specificity genes, organ specificity genes and some detoxification metabolic genes among these four cereal crop aphids. Meanwhile, their expression patterns were showed. The related functions and pathways of CYP450s were revealed by GO and KEGG enrichment analysis. Above all, we picked the differentially expressed CYP450 genes from all of the differentially expressed genes (DEGs). These differentially expressed CYP450 genes provided some new potential candidates for aphid control and management. This work establishes the foundation for further investigations into the regulatory functions of the CYP450 gene family in aphid species and beyond.

Ferroptosis hallmarked by lipid peroxidation and iron homeostasis imbalance is involved in the occurrence and development of various diseases. The plant growth regulator chlormequat chloride (CCC) can contribute to the causality and exacerbation of reproductive disorders. However, the mechanism by which CCC may cause Leydig cell attenuation remains poorly understood. In this study, TM3 Leydig cells were used to investigate the inhibitory effect of CCC on cell growth and its possible mechanism. The results showed that CCC caused apoptosis, pyroptosis, ferroptosis and necroinflammation in TM3 cells. By comparing the effects of ferroptosis inhibitor Ferrostatin-1 (Fer-1) and pan-Caspase inhibitor Z-VAD-FMK (ZVF) on lipid peroxidation and Caspase-mediated regulated cell death (RCD), we found that Fer-1 was better at rescuing the growth of TM3 cells than ZVF. Although ZVF reduced mitochondrial ROS level and inhibited the activation of Caspase3 and Caspase1, it could not significantly ameliorate lipid peroxidation and the levels of IL-1β and HMGB1 like Fer-1. Therefore, ferroptosis might be a key non apoptotic RCD mode responsible for CCC-driven inflammation, leading to weakened viability and proliferation of TM3 cells. In addition, overexpression of ferritin light chain (FTL) promoted the resistance of TM3 cells to CCC-induced ferroptosis-mediated inflammation and to some extent improved the inhibition of viability and proliferation. Altogether, ferroptosis-initiated inflammation might play a key role in CCC-impaired TM3 cell growth.

Acute myeloid leukemia (AML) is a hematologic malignancy characterized by infiltration of the blood and bone marrow, exhibiting a low remission rate and high recurrence rate. Current research has demonstrated that class I HDAC inhibitors can downregulate anti-apoptotic proteins, leading to apoptosis of AML cells. In the present investigation, we conducted structural modifications of marine cytotoxin Santacruzamate A (SCA), a compound known for its inhibitory activity towards HDACs, resulting in the development of a novel series of potent class I HDACs hydrazide inhibitors. Representative hydrazide-based compound 25c exhibited concentration-dependent induction of apoptosis in AML cells as a single agent. Moreover, 25c exhibited a synergistic anti-AML effect when combined with Venetoclax, a clinical Bcl-2 inhibitor employed in AML therapy. This combination resulted in a more pronounced downregulation of anti-apoptotic proteins Mcl-1 and Bcl-xL, along with a significant upregulation of the pro-apoptotic protein cleaved-caspase3 and the DNA double-strand break biomarker γ-H2AX compared to monotherapy. These results highlighted the potential of 25c as a promising lead compound for AML treatment, particularly when used in combination with Venetoclax.

Research has established that the incorporation of 3D-printed lattice structures in cement substrates enhances the mechanical properties of cementitious materials. However, given that 3D-printing materials, notably polymers, exhibit varying degrees of mechanical performance under high-temperature conditions, their efficacy is compromised. Notably, at temperatures reaching 150 °C, these materials soften and lose their load-bearing capacity, necessitating further investigation into their compressive mechanical behavior in such environments. This study evaluates the compressibility of cement materials reinforced with lattice structures made from polyamide 6 (PA6) across different structural configurations and ambient temperatures, employing ABAQUS for simulation. Six distinct 3D-printed lattice designs with equivalent volume but varying configurations were tested under ambient temperatures of 20 °C, 50 °C, and 100 °C to assess their impact on compressive properties. The findings indicate that heightened ambient temperatures significantly diminish the reinforcing effect of 3D-printed materials on the properties of cement-based composites., Competing Interests: The authors declare no conflict of interest.

In this paper, we consider a downlink non-orthogonal multiple access (NOMA) system over Nakagami- m channels. The single-antenna base station serves two single-antenna NOMA users based on statistical channel state information (CSI). We derive the closed-form expression of the exact outage probability under a given decoding order, and we also deduce the asymptotic outage probability and diversity order in a high-SNR regime. Then, we analyze all the possible power allocation ranges and theoretically prove the optimal power allocation range under the corresponding decoding order. The demarcation points of the optimal power allocation ranges are affected by target data rates and total power, without an effect from the CSI. In particular, the values of the demarcation points are proportional to the total power. Furthermore, we formulate a joint decoding order and power allocation optimization problem to maximize the sum throughput, which is solved by efficiently searching in our obtained optimal power allocation ranges. Finally, Monte Carlo simulations are conducted to confirm the accuracy of our derived exact outage probability. Numerical results show the accuracy of our deduced demarcation points of the optimal power allocation ranges. And the optimal decoding order is not constant at different total transmit power levels.

The excretion care robot's (ECR) accurate recognition of transfer-assisted actions is crucial during its usage. However, transfer action recognition is a challenging task, especially since the differentiation of actions seriously affects its recognition speed, robustness, and generalization ability. We propose a novel approach for transfer action recognition assisted by a bidirectional long- and short-term memory (Bi-LSTM) network combined with a multi-head attention mechanism. Firstly, we utilize posture sensors to detect human movements and establish a lightweight three-dimensional (3D) model of the lower limbs. In particular, we adopt a discrete extended Kalman filter (DEKF) to improve the accuracy and foresight of pose solving. Then, we construct an action prediction model that incorporates a fused Bi-LSTM with Multi-head attention (MHA Bi-LSTM). The MHA extracts key information related to differentiated movements from different dimensions and assigns varying weights. Utilizing the Bi-LSTM network effectively combines past and future information to enhance the prediction results of differentiated actions. Finally, comparisons were made by three subjects in the proposed method and with two other time series based neural network models. The reliability of the MHA Bi-LSTM method was verified. These experimental results show that the introduced MHA Bi-LSTM model has a higher accuracy in predicting posture sensor-based excretory care actions. Our method provides a promising approach for handling transfer-assisted action individual differentiation in excretion care tasks.

Sodium-ion batteries have important application prospects in large-scale energy storage due to their advantages, such as safety, affordability, and abundant resources. Prussian blue analogs (PBAs) have a stable and open framework structure, making them a very promising cathode material. However, high-performance manganese-based Prussian blue cathode materials for sodium-ion batteries still suffer from significant challenges due to several key issues, such as a high number of vacancy defects and a high crystal water content. This article investigates the effects of the Fe-Mn molar ratio, Mn ion concentration, and reaction time on the electrochemical performance of MnHCF during the coprecipitation process. When Fe:Mn = 1:2, c(Mn 2+ ) = 0.02 mol/L, and the reaction time is 12 h, the content of interstitial water molecules in the sample is low, and the Fe(CN) 6 defects are few. At 0.1 C, the prepared electrode has a high initial discharge specific capacity (121.9 mAh g -1 ), and after 100 cycles at 0.2 C, the capacity retention rate is 65% (~76.2 mAh g -1 ). Meanwhile, the sample electrode exhibits excellent reversibility. The discharge capacity can still be maintained at around 75% when the magnification is restored from 5 C to 0.1 C. The improvement in performance is mainly attributed to two aspects: On the one hand, reducing the Fe(CN) 6 defects and crystal water content is conducive to the diffusion and stable structure of N. On the other hand, reducing the reaction rate can significantly delay the crystallization of materials and optimize the nucleation process.

Finding new agents to enhance the strength of alkali-activated (ground granulated blast furnace slag) GGBS is beneficial for environmental protection. Here, we reveal the effect of pregelatinized starch-modifying calcium silicate hydrate (C-S-H) particles on the nanostructure tailoring of NaOH-activated GGBS hydrates. The results show that, for the synthetic modified C-S-H, the pregelatinized starch absorbs on the surface of C-S-H, which modifies the silicate chains and crystal structure. Adding pregelatinized starch-modified C-S-H particles can tailor the chemical structure of calcium silicaluminate hydrate (C-A-S-H) formed from GGBS hydration by increasing the mean chain length (MCL) and decreasing the Al/Si ratios. When adding C-S-H particles modified by 0.1% pregelatinized starch, the MCL of C-A-S-H is increased by 344.5% and the Al/Si ratio is decreased by 16.0%. The compressive strength of NaOH-activated GGBS samples can be enhanced by adding pregelatinized starch-modified C-S-H particles, while the addition for modified C-S-H does not significantly affect the flexural strength. The high strength of hardened blocks of hydrated GGBS is related to the long MCL silicate chains. These findings provide a potential application of pregelatinized starch-modifying C-S-H particle acting as strength-enhancing agents.

The alkali-activated cementitious material was prepared by partially replacing slag with recycled concrete powder (RCP). The influence of RCP substitution rates (10%, 20%, 30%, 40%, and 50% mass fraction) on the performance of alkali-activated slag-RCP-based (AASR) foamed concrete was studied. The fluidity, water absorption, softening coefficient, compressive strength, flexural strength, drying shrinkage, thermal conductivity, and frost resistance of AASR foamed concrete were studied. The results show that the fluidity and softening coefficient of AASR foamed concrete decreases with the increase in RCP content, and the fluidity range is 230-270 mm. Due to the porous structure of the RCP, the water absorption of AASR increases. With the increase in the curing age, the strength of AASR foamed concrete increases. The addition of RCP reduced the mechanical properties of AASR foamed concrete. Compared with the control group, the compressive strength of AASR50 decreased by 66.7% at 28 days, and the flexural strength decreased by 61.5%. However, the 28 d compressive strength of AASR foamed concrete under all RCP replacement rates still meets the standard value (0.6 MPa). The addition of RCP effectively reduces the thermal conductivity of the AASR foamed concrete, and when the RCP content is 50%, the thermal conductivity is lowest, 0.119 W/(m·K); the drying shrinkage of the AASR foamed concrete can be improved by adding RCP, and the drying shrinkage value is lowest when the RCP is 30%, which is 14.7% lower than that of the control group. The frost resistance of AASR foamed concrete decreases with the increase in the RCP content. When the recycled micropowder content is 20-50% and after 25 freeze-thaw cycles, AASR foamed concrete has reached freeze-thaw damage.

Interleukin-21 and its receptors (IL-21/IL-21R) aggravate chlamydial lung infection, while macrophages (Mφ) are one of the main cells infected by chlamydia and the main source of inflammatory cytokines. Therefore, it is particularly important to study whether IL-21/IL-21R aggravates chlamydia respiratory infection by regulating Mφ. Combined with bioinformatics analysis, we established an IL-21R-deficient (IL-21R -/- ) mouse model of Chlamydia muridarum ( C. muridarum ) respiratory tract infection in vivo, studied C. muridarum -stimulated RAW264.7 by the addition of rmIL-21 in vitro, and conducted adoptive transfer experiments to clarify the association between IL-21/IL-21R and Mφ. IL-21R -/- mice showed lower infiltration of pulmonary total Mφ, alveolar macrophages, and interstitial macrophages compared with WT mice following infection. Transcriptomic analysis suggested that M1-related genes are downregulated in IL-21R -/- mice and that IL-21R deficiency affects the Mφ-mediated inflammatory response during C. muridarum infection. In vivo experiments verified that in IL-21R -/- mice, pulmonary M1-type CD80 + , CD86 + , MHC II + , TNFα + , and iNOS + Mφ decreased, while there were no differences in M2-type CD206 + , TGF-β + , IL-10 + and ARG1 + Mφ. In vitro, administration of rmIL-21 to C. muridarum -stimulated RAW264.7 cells promoted the levels of iNOS-NO and the expression of IL-12p40 and TNFα, but had no effect on TGFβ or IL-10. Further, adoptive transfer of M1-like bone marrow-derived macrophages derived from IL-21R -/- mice, unlike those from WT mice, effectively protected the recipients against C. muridarum infection and induced relieved pulmonary pathology. These findings help in understanding the mechanism by which IL-21/IL-21R exacerbates chlamydia respiratory infection by promoting the proinflammatory effect of Mφ.

Disulfide bonds are dynamic covalent bonds, which are easy to cleave and reform upon chemical stimulus. Various methods including the oxidative coupling of thiols and polymerization of disulfide-containing monomers have been developed for the synthesis of poly(disulfide)s. However, installing small amounts of disulfide units in the main chain of polyolefins has received much less attention. Herein, we report a novel strategy for incorporating cleavable disulfide units into the backbone of polyolefins using commercially available diallyl disulfide (DADS) as a comonomer via metathesis copolymerization. The copolymerization of diallyl disulfide with cyclooctene occurred using the second-generation Grubbs catalyst under mild conditions, allowing for the synthesis of copolymers with adjustable disulfide content ranging from 0.7 to 8.5 mol%, and the molecular weight of the obtained copolymers ranged from 5.8 kg·mol -1 to 42.8 kg·mol -1 . The resulting polyolefins with disulfide insertion retained excellent thermal processability and exhibited degradability. Treatment of the copolymer (8.5 mol% disulfide content) with tri- n -butylphosphine resulted in a significant reduction in molecular weight from 5.8 kg·mol -1 to 1.6 kg·mol -1 . Successful copolymerization with diallyl disulfide provides a convenient and effective method for obtaining degradable polyolefins.

Long-chain acyl-CoA synthetase 1 (ACSL1) plays an important role in fatty acid metabolism and fat deposition. The transcription of the ACSL1 gene is regulated specifically among cells and physiological processes, and transcriptional regulation of ACSL1 in adipogenesis remains elusive. Here, we characterize transcription factors (TFs) associated with adipogenesis in the porcine ACSL1 gene. CCAAT-enhancer binding protein (C/EBP)α, a well-known adipogenic marker, was found to enhance the expression of the ACSL1 gene via binding two tandem motifs in the promoter. Further, we demonstrate that ACSL1 mediates C/EBPα effects on adipogenesis in preadipocytes cultured from subcutaneous fat tissue of pigs via gain- and loss-of-function analyses. The cAMP-response element binding protein, another TF involved in adipogenesis, was also identified in the regulation of ACSL1 gene expression. Additionally, single nucleotide polymorphisms (SNPs) were screened in the promoter of ACSL1 among four breeds including the Chinese indigenous Min, and Duroc, Berkshire, and Yorkshire pigs through sequencing of PCR products. Two tightly linked SNPs, -517G>T and -311T>G, were found exclusively in Min pigs. The haplotype mutation decreases promoter activity in PK-15 and ST cells, and in vivo the expression of ACSL1 , illustrating a possible role in adipogenesis regulated by C/EBPα/ACSL1 axis. Additionally, a total of 24 alternative splicing transcripts were identified, indicating the complexity of alternative splicing in the ACSL1 gene. The results will contribute to further revealing the regulatory mechanisms of ACSL1 during adipogenesis and to the characterization of molecular markers for selection of fat deposition in pigs.

The need to reach carbon neutrality as soon as possible has made the use of recycled materials widespread. However, the treatment of artificial marble waste powder (AMWP) containing unsaturated polyester is a very challenging task. This task can be accomplished by converting AMWP into new plastic composites. Such conversion is a cost-effective and eco-friendly way to recycle industrial waste. However, the lack of mechanical strength in composites and the low filling content of AMWP have been major obstacles to its practical application in structural and technical buildings. In this study, a composite of AMWP/linear low-density polyethylene (LLDPE) filled with a 70 wt% AMWP content was fabricated using maleic anhydride-grafted polyethylene as a compatibilizer (MAPE). The mechanical strength of the prepared composites is excellent (tensile strength ~18.45 MPa, impact strength ~51.6 kJ/m 2 ), making them appropriate as useful building materials. Additionally, laser particle size analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy, and thermogravimetric analysis were used to examine the effects of maleic anhydride-grafted polyethylene on the mechanical properties of AMWP/LLDPE composites and its mechanism of action. Overall, this study offers a practical method for the low-cost recycling of industrial waste into high-performance composites.

Changes in pig behavior are crucial information in the livestock breeding process, and automatic pig behavior recognition is a vital method for improving pig welfare. However, most methods for pig behavior recognition rely on human observation and deep learning. Human observation is often time-consuming and labor-intensive, while deep learning models with a large number of parameters can result in slow training times and low efficiency. To address these issues, this paper proposes a novel deep mutual learning enhanced two-stream pig behavior recognition approach. The proposed model consists of two mutual learning networks, which include the red-green-blue color model (RGB) and flow streams. Additionally, each branch contains two student networks that learn collaboratively to effectively achieve robust and rich appearance or motion features, ultimately leading to improved recognition performance of pig behaviors. Finally, the results of RGB and flow branches are weighted and fused to further improve the performance of pig behavior recognition. Experimental results demonstrate the effectiveness of the proposed model, which achieves state-of-the-art recognition performance with an accuracy of 96.52%, surpassing other models by 2.71%.

Developing flame-retarded styrene-acrylic emulsion (SAE) based damping composites is a challenging task because of their very high flammability. A promising approach is the synergistic combination of expandable graphite (EG) and ammonium polyphosphate (APP). In this study, the surface modification of APP was modified by commercial titanate coupling agent ndz-201 through ball milling, and the SAE-based composite material was prepared with SAE and different ratios of modified ammonium polyphosphate (MAPP) and EG. The surface of MAPP was successfully chemically modified by NDZ-201 through scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), Energy Dispersion Spectroscopy (EDS), and contact angle. The effects of different ratios of MAPP and EG on the dynamic and static mechanical properties and flame retardancy of composite materials were explored. The results showed that when MAPP:EG = 1:4, the limiting oxygen index (LOI) of the composite material was 52.5%, and the vertical burning test (UL-94) was at the V0 level. Its LOI increased by 141.9% compared to the composite materials without flame retardant. The optimized formulation of MAPP and EG in SAE-based damping composite materials showed a significant synergistic effect on the flame retardancy of the composite material.

The FeCrSiNiCoC coatings with fine macroscopic morphology and uniform microstructure were made on 1Cr11Ni heat resistant steel substrate by a laser-based cladding technique. The coating consists of dendritic γ-Fe and eutectic Fe-Cr intermetallic with an average microhardness of 467 HV 0.5 ± 22.6 HV 0.5 . At the load of 200 N, the average friction coefficient of the coating dropped as temperature increased, while the wear rate decreased and then increased. The wear mechanism of the coating changed from abrasive wear, adhesive wear and oxidative wear to oxidative wear and three-body wear. Apart from an elevation in wear rate with increasing load, the mean friction coefficient of the coating hardly changed at 500 °C. Due to the coating's transition from adhesive wear and oxidative wear to three-body wear and abrasive wear, the underlying wear mechanism also shifted.

Chitooligosaccharide (COS) is a green and non-toxic cationic carbohydrate that has attracted wide attention in recent years due to its anti-inflammatory activity. However, the anti-inflammatory mechanism of COS remains unclear. In this study, RNA-seq was used to investigate the integrated response of COS to LPS-induced damage in macrophages. The results showed that the experimental group with COS had 2570 genes with significant differences compared to the model group, and that these genes were more enriched in inflammatory and immune pathways. The KEGG results showed that COS induces the pleiotropic modulation of classical inflammatory pathways, such as the Toll-like receptor signaling pathway, NF-κB, MAPK, etc. Based on the RNA-seq data and the RT-qPCR, as well as the WB validation, COS can significantly upregulate the expression of membrane receptors, such as Tlr4, Tlr5, and MR, and significantly inhibits the phosphorylation of several important proteins, such as IκB and JNK. Overall, this study offers deep insights into the anti-inflammatory mechanism and lays the foundation for the early application of COS as an anti-inflammatory drug.

The impact response of a composite structure consisting of a metal-packaged ceramic interlayer and an ultra-high molecular weight polyethylene (UHMWPE) laminate has been studied through a ballistic test and numerical simulation. The studied structure exhibits 50% higher anti-penetration performance than the traditional ceramic/metal structure with the same areal density. The metal-packaged ceramic interlayer and the UHMWPE laminate are key components in resisting the penetration. By using a metal frame to impose three-dimensional constraints on ceramic tiles, the metal-packaged ceramic interlayer can limit the crushing of the ceramic and contain the broken ceramic fragment to improve the erosion of the projectile. The large deformation of UHMWPE laminate absorbs a large amount of energy from the projectile. By decreasing the amplitude of the shock wave and changing the distribution of the impact load in the structure, the projectile has longer residence time on the interlayer. The anti-penetration performance shows within 10% variation when the impact position is varied. Due to the asymmetric deformation and high elastic recovery ability of the UHMWPE laminate, the projectile trajectory deflection is increased, and the broken ceramic fragments are restrained, thereby mitigating after-effect damage caused by the projectile after penetrating the structure.

SiC p /AZ91D magnesium matrix composites with 30% SiC p were successfully prepared by pulsed current melting in this work. Then, the influences of the pulse current on the microstructure, phase composition, and heterogeneous nucleation of the experimental materials were analyzed in detail. The results show that the grain size of both the solidification matrix structure and SiC reinforcement are refined by pulse current treatment, and the refining effect is gradually more obvious with an increase in the pulse current peak value. Moreover, the pulse current reduces the chemical potential of the reaction between SiC p and Mg matrix, thus promoting the reaction between SiC p and the alloy melt and stimulating the formation of Al 4 C 3 along the grain boundaries. Furthermore, Al 4 C 3 and MgO, as heterogeneous nucleation substrates, can induce heterogeneous nucleation and refine the solidification matrix structure. Finally, when increasing the peak value of the pulse current, the repulsive force between the particles increases while the agglomeration phenomenon is suppressed, which results in the dispersed distribution of SiC reinforcements.

Photodynamic therapy (PDT) is a treatment that employs exogenously produced reactive oxygen species (ROS) to kill cancer cells. ROS are generated from the interaction of excited-state photosensitizers (PSs) or photosensitizing agents with molecular oxygen. Novel PSs with high ROS generation efficiency is essential and highly required for cancer photodynamic therapy. Carbon dots (CDs), the rising star of carbon-based nanomaterial family, have shown great potential in cancer PDT benefiting from their excellent photoactivity, luminescence properties, low price, and biocompatibility. In recent years, photoactive near-infrared CDs (PNCDs) have attracted increasing interest in this field due to their deep therapeutic tissue penetration, superior imaging performance, excellent photoactivity, and photostability. In this review, we review recent progress in the designs, fabrication, and applications of PNCDs in cancer PDT. We also provide insights of future directions in accelerating the clinical progress of PNCDs.

DNA methylation is an epigenetic modification that plays a vital role in a variety of biological processes, including the regulation of gene expression, cell differentiation, early embryonic development, genomic imprinting, and X chromosome inactivation. PGC7 is a maternal factor that maintains DNA methylation during early embryonic development. One mechanism of action has been identified by analyzing the interactions between PGC7 and UHRF1, H3K9 me2, or TET2/TET3, which reveals how PGC7 regulates DNA methylation in oocytes or fertilized embryos. However, the mechanism by which PGC7 regulates the post-translational modification of methylation-related enzymes remains to be elucidated. This study focused on F9 cells (embryonic cancer cells), which display high levels of PGC7 expression. We found that both knockdown of Pgc7 and inhibition of ERK activity resulted in increased genome-wide DNA methylation levels. Mechanistic experiments confirmed that inhibition of ERK activity led to the accumulation of DNMT1 in the nucleus, ERK phosphorylated DNMT1 at ser717, and DNMT1 Ser717-Ala mutation promoted the nuclear localization of DNMT1. Moreover, knockdown of Pgc7 also caused downregulation of ERK phosphorylation and promoted the accumulation of DNMT1 in the nucleus. In conclusion, we reveal a new mechanism by which PGC7 regulates genome-wide DNA methylation via phosphorylation of DNMT1 at ser717 by ERK. These findings may provide new insights into treatments for DNA methylation-related diseases.

The vertebrate photoperiodic neuroendocrine system uses the photoperiod as a proxy to time the annual rhythms in reproduction. The thyrotropin receptor (TSHR) is a key protein in the mammalian seasonal reproduction pathway. Its abundance and function can tune sensitivity to the photoperiod. To investigate seasonal adaptation in mammals, the hinge region and the first part of the transmembrane domain of the Tshr gene were sequenced for 278 common vole ( Microtus arvalis ) specimens from 15 localities in Western Europe and 28 localities in Eastern Europe. Forty-nine single nucleotide polymorphisms (SNPs; twenty-two intronic and twenty-seven exonic) were found, with a weak or lack of correlation with pairwise geographical distance, latitude, longitude, and altitude. By applying a temperature threshold to the local photoperiod-temperature ellipsoid, we obtained a predicted critical photoperiod (pCPP) as a proxy for the spring onset of local primary food production (grass). The obtained pCPP explains the distribution of the genetic variation in Tshr in Western Europe through highly significant correlations with five intronic and seven exonic SNPs. The relationship between pCPP and SNPs was lacking in Eastern Europe. Thus, Tshr , which plays a pivotal role in the sensitivity of the mammalian photoperiodic neuroendocrine system, was targeted by natural selection in Western European vole populations, resulting in the optimized timing of seasonal reproduction.

Development from single cells to multicellular tissues and organs involves more than just the exact replication of cells, which is known as differentiation. The primary focus of research into the mechanism of differentiation has been differences in gene expression profiles between individual cells. However, it has predominantly been conducted at low throughput and bulk levels, challenging the efforts to understand molecular mechanisms of differentiation during the developmental process in animals and humans. During the last decades, rapid methodological advancements in genomics facilitated the ability to study developmental processes at a genome-wide level and finer resolution. Particularly, sequencing transcriptomes at single-cell resolution, enabled by single-cell RNA-sequencing (scRNA-seq), was a breath-taking innovation, allowing scientists to gain a better understanding of differentiation and cell lineage during the developmental process. However, single-cell isolation during scRNA-seq results in the loss of the spatial information of individual cells and consequently limits our understanding of the specific functions of the cells performed by different spatial regions of tissues or organs. This greatly encourages the emergence of the spatial transcriptomic discipline and tools. Here, we summarize the recent application of scRNA-seq and spatial transcriptomic tools for developmental biology. We also discuss the limitations of current spatial transcriptomic tools and approaches, as well as possible solutions and future prospects.

Mycotoxins are ubiquitously present in feeds and raw materials and can exert toxicity on animals and humans. Therefore, mycotoxin occurrence should be monitored. We report here a multi-mycotoxin survey of feed samples in China from 2017 to 2021. Concentrations of aflatoxins, trichothecenes type B, fumonisins, and zearalenone were determined in a total of 9392 samples collected throughout China. Regional differences and year-to-year variation of mycotoxin occurrence were also assessed in new-season corn. Generally, Fusarium mycotoxins were prevalent, while mycotoxin contamination in each feed commodity showed a distinct pattern, e.g., wheat and bran were typically affected by trichothecenes type B, peanut meals were highly susceptible to aflatoxins, and finished feeds exhibited a comparatively high prevalence of all mycotoxins. In new-season corn, trichothecenes type B and fumonisins were most prevalent, with positive rates of 84.04% and 87.16%, respectively. Regions exhibited different patterns of mycotoxin occurrence. The Anhui and Jiangsu provinces of East China exhibited a high prevalence and concentrations of aflatoxins with a positive rate and a positive average of 82.61% and 103.08 μg/kg, respectively. Central China obtained high fumonisins levels of 4707.84 μg/kg. Trichothecenes type B and zearalenone occurred more frequently in temperate regions of Northeast China, and their positive rates reached 94.99% and 55.67%, respectively. In these regions, mycotoxin concentrations in new-season corn exhibited pronounced year-to-year variations and this could be due to the unusual changes of rainfall or temperature during sensitive periods of corn growing. A large fraction of new-season corn samples contained multiple mycotoxins with two to three classes (75.42%), and the most frequently observed co-contaminants were the combination of trichothecenes type B and fumonisins (73.52%). Trichothecenes type B and zearalenone concentrations were highly positively correlated with a coefficient of 0.775. In conclusion, mycotoxins contamination and co-contamination of feeds are common. Mycotoxin contamination in new-season corn exhibited regional patterns and year-to-year variations, with climate and weather conditions as determinant factors.

Dysfunction of the lenticular nucleus is thought to contribute to neurological symptoms in Wilson's disease (WD). However, very little is known about whether and how the lenticular nucleus influences dystonia by interacting with the cerebral cortex and cerebellum. To solve this problem, we recruited 37 WD patients (20 men; age, 23.95 ± 6.95 years; age range, 12-37 years) and 37 age- and sex-matched healthy controls (HCs) (25 men; age, 25.19 ± 1.88 years; age range, 20-30 years), and each subject underwent resting-state functional magnetic resonance imaging (RS-fMRI) scans. The muscle biomechanical parameters and Unified Wilson Disease Rating Scale (UWDRS) were used to evaluate the level of dystonia and clinical representations, respectively. The lenticular nucleus, including the putamen and globus pallidus, was divided into 12 subregions according to dorsal, ventral, anterior and posterior localization and seed-based functional connectivity (FC) was calculated for each subregion. The relationships between FC changes in the lenticular nucleus with muscle tension levels and clinical representations were further investigated by correlation analysis. Dystonia was diagnosed by comparing all WD muscle biomechanical parameters with healthy controls (HCs). Compared with HCs, FC decreased from all subregions in the putamen except the right ventral posterior part to the middle cingulate cortex (MCC) and decreased FC of all subregions in the putamen except the left ventral anterior part to the cerebellum was observed in patients with WD. Patients with WD also showed decreased FC of the left globus pallidus primarily distributed in the MCC and cerebellum and illustrated decreased FC from the right globus pallidus to the cerebellum. FC from the putamen to the MCC was significantly correlated with psychiatric symptoms. FC from the putamen to the cerebellum was significantly correlated with muscle tension and neurological symptoms. Additionally, the FC from the globus pallidus to the cerebellum was also associated with muscle tension. Together, these findings highlight that lenticular nucleus-cerebellum circuits may serve as neural biomarkers of dystonia and provide implications for the neural mechanisms underlying dystonia in WD.

Gandouling (GDL) Pill is a novel Traditional Chinese medicinal drug to treat Wilson's disease in clinics. It is composed of six separate herbal medicines, including Rhei Radix ET Rhizoma, Coptidis Rhizoma, Salviae Miltiorrhizae Radix ET Rhizoma, Spatholobi Caulis, Curcumae Rhizoma, and Curcumae Longae Rhizoma. In this study, a strategy was proposed to investigate the chemical constituents and to quantify the potential bioactive components in GDL Pill. Firstly, the mass fragmentation behaviors of representative compounds were investigated, and, in total, 69 compounds were characterized in GDL Pill using full scan/dd-MS 2 scan mode by ultra-high-performance liquid chromatography (UPLC)/Q-Orbitrap mass spectrometry (MS). These compounds included 18 alkaloids, 18 ketones, 16 phenolic compounds, 11 organic acids, and 6 tanshinones. Seventeen of the compounds were unambiguously identified by comparison with reference standards. Secondly, the absorption components of GDL Pill in rat plasma were investigated by using target-Selected Ion Monitoring (t-SIM) scan mode built in Q-Orbitrap MS. A total of 18 components were detected, which were considered as potential bioactive components of GDL Pill. Thirdly, 10 major absorption components were simultaneously determined in six batches of samples by UPLC/diode array detector (DAD). The method was fully validated with respect to linearity, precision, repeatability, stability, and recovery. Alkaloids from Coptidis Rhizoma, such as coptisine ( 8 ), berberine ( 18 ), palmatine ( 19 ), were the most abundant bioactive compounds for GDL Pill that possess the potential be used as quality markers. The proposed strategy is practical and efficient for revealing the material basis of GDL Pill, and also provides a simple and accurate method for quality control.

Pork is the most widely consumed meat product in the world, and achieving accurate detection of individual pigs is of great significance for intelligent pig breeding and health monitoring. Improved pig detection has important implications for improving pork production and quality, as well as economics. However, most of the current approaches are based on manual labor, resulting in unfeasible performance. In order to improve the efficiency and effectiveness of individual pig detection, this paper describes the development of an attention module enhanced YOLOv3-SC model (YOLOv3-SPP-CBAM. SPP denotes the Spatial Pyramid Pooling module and CBAM indicates the Convolutional Block Attention Module). Specifically, leveraging the attention module, the network will extract much richer feature information, leading the improved performance. Furthermore, by integrating the SPP structured network, multi-scale feature fusion can be achieved, which makes the network more robust. On the constructed dataset of 4019 samples, the experimental results showed that the YOLOv3-SC network achieved 99.24% mAP in identifying individual pigs with a detection time of 16 ms. Compared with the other popular four models, including YOLOv1, YOLOv2, Faster-RCNN, and YOLOv3, the mAP of pig identification was improved by 2.31%, 1.44%, 1.28%, and 0.61%, respectively. The YOLOv3-SC proposed in this paper can achieve accurate individual detection of pigs. Consequently, this novel proposed model can be employed for the rapid detection of individual pigs on farms, and provides new ideas for individual pig detection.

High tensile strength and toughness play an important role in improving the mechanical performance of separator films, such as resistance to external force, improving service life, etc. In this study, a nanoindentation experiment is performed to investigate the mechanical properties of two types of separators for LIBs based on the grid nanoindentation method. During the indentation experiment, the "sink-in" phenomenon is observed around the indenter when plastic deformation of the specimen occurs. The "sink-in" area of the polyethylene (PE) separator is larger than that of the polypropylene/polyethylene/polypropylene (PP/PE/PP) separator, i.e., the plastic area of the PE separator is larger than that of the PP/PE/PP separator. In order to select a suitable method to evaluate the hardness and elastic modulus of these separators for LIBs, three theoretical methods, including the Oliver-Pharr method, the indentation work method, and the fitting curve method, are used for analysis and comparison in this study. The results obtained by the fitting curve method are more reasonable and accurate, which not only avoids the problem of the large contact area obtained by the Oliver-Pharr method, but also avoids the influence caused by the large fitting data of the displacement-force curve and the inaccuracy of using the maximum displacement obtained by the indentation method. In addition, the obstruction ability of the PP/PE/PP separator to locally resist external load pressed into its surface and to resist micro particles, such as fine metal powder, that can enter the lithium-ion battery during the manufacturing process is greater than that of the PE separator. This research provides guidance for studying the mechanical properties and exploring the estimation method of macromolecular separators for LIBs.

Endotoxemia is mainly caused by a massive burst of inflammatory cytokines as a result of lipopolysaccharide (LPS) invasion. Chitooligosaccharides (COS) is expected to be a potential drug for relieving endotoxemia due to its anti-inflammatory properties. However, the structural parameters of COS are often ambiguous, and the effect of degree of acetylation (DA) of COS on its anti-inflammatory remains unknown. In this study, four COSs with different DAs (0%, 12%, 50% and 85%) and the same oligomers distribution were successfully obtained. Their structures were confirmed by 1 H NMR and MS analysis. Then, the effect of DA on the anti-inflammatory activity and relieving endotoxemia potential of COS was researched. The results revealed that COS with a DA of 12% had better anti-inflammatory activity than COSs with other DAs, mainly in inhibiting LPS-induced inflammatory cytokines burst, down-regulating its mRNA expression and reducing phosphorylation of IκBα. Furthermore, this COS showed an obviously protective effect on endotoxemia mice, such as inhibiting the increase in inflammatory cytokines and transaminases, alleviating the injury of liver and intestinal tissue. This study explored the effect of DA on the anti-inflammatory activity of COS for the first time and lays the foundation for the development of COS as an anti-inflammatory drug against endotoxemia.

The cAMP response element-binding protein (CREB), a basic leucine zipper transcription factor, is involved in the activation of numerous genes in a variety of cell types. The CREB gene is rich in alternative splicing (AS) events. However, studies on the AS of CREB genes in pigs are limited, and few reports have compared the roles of isoforms in activating gene expression. Here, five AS transcripts, V1-5, were characterized by RT-PCR and two, V3 and V5, were new identifications. Both V1 and V2 have all the functional domains of the CREB protein, with similar tissue expression profiles and mRNA stability, suggesting that they have similar roles. The transcriptional transactivation activities of four isoforms encoding complete polypeptides were analyzed on the expression of the B-cell CLL/lymphoma 2-like protein 2 and the poly (A)-binding protein, nuclear 1 genes with a dual-luciferase reporter system, and differential activities were observed. Both V1 and V2 have promoting effects, but their roles are gene-specific. V3 has no effect on the promoter of the two genes, while V4 functions as a repressor. The mechanisms underlying the differential roles of V1 and V2 were analyzed with RNA-seq, and the genes specifically regulated by V1 and V2 were identified. These results will contribute to further revealing the role of CREB and to analyzing the significance of AS in genes., Competing Interests: The authors declare no conflict of interest.

The objective of this study was to investigate the protective effects and potential molecular mechanisms of procyanidin B2 (PB2) in MAC-T (mammary alveolar cells-large T antigen) cells during heat stress (HS). The MAC-T cells were divided into three treatment groups: control (37 °C), HS (42 °C), and PB2 + HS (42 °C). Compared with MAC-T cells that were consistently cultured at 37 °C, acute HS treatment remarkably decreased cell viability, reduced activities of catalase (CAT), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC), and elevated intracellular levels of malondialdehyde (MDA) and reactive oxygen species (ROS). Additionally, nuclear factor erythroid 2-related factor 2 (Nrf2) was activated and translocated to the nucleus, in accompaniment with upregulation of Nrf2, heme oxygenase 1 (HO-1), thioredoxin reductase 1 (Txnrd1), and heat shock protein 70 (HSP70). In parallel, both mRNA transcript and actual protein secretion of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), were increased by heat stress. Pretreatment of MAC-T cells with 0~25 μM PB2 alleviated the decline of cell viability by HS in a dose-dependent fashion and protected cells against HS-induced oxidative stress, as evidenced by significantly improved CAT, SOD, and T-AOC activity, as well as with decreased MDA and ROS generation. Furthermore, PB2 further activated the Nrf2 signaling pathway and reversed the inflammatory response induced by HS. Silencing of Nrf2 by si-Nrf2 transfection not only exacerbated HS-induced cell death and provoked oxidative stress and the inflammatory response, but also greatly abolished the cytoprotective effects under HS of PB2. In summary, PB2 protected MAC-T cells against HS-induced cell death, oxidative stress, and inflammatory response, partially by operating at the Nrf2 signal pathway.

Floral colour is an important agronomic trait that influences the commercial value of ornamental plants. Anthocyanins are a class of flavonoids and confer diverse colours, and elucidating the molecular mechanisms that regulate their pigmentation could facilitate artificial manipulation of flower colour in ornamental plants. Here, we investigated the regulatory mechanism of light-induced anthocyanin biosynthesis during flower colouration in grape hyacinth ( Muscari spp.). We studied the function of two B-box proteins, MaBBX20 and MaBBX51. The qPCR revealed that MaBBX20 and MaBBX51 were associated with light-induced anthocyanin biosynthesis. Both MaBBX20 and MaBBX51 are transcript factors and are specifically localised in the nucleus. Besides, overexpression of MaBBX20 in tobacco slightly increased the anthocyanin content of the petals, but reduced in MaBBX51 overexpression lines. The yeast one-hybrid assays indicated that MaBBX20 and MaBBX51 did not directly bind to the MaMybA or MaDFR promoters, but MaHY5 did. The BiFC assay revealed that MaBBX20 and MaBBX51 physically interact with MaHY5. A dual luciferase assay further confirmed that the MaBBX20-MaHY5 complex can strongly activate the MaMybA and MaDFR transcription in tobacco. Moreover, MaBBX51 hampered MaBBX20-MaHY5 complex formation and repressed MaMybA and MaDFR transcription by physically interacting with MaHY5 and MaBBX20. Overall, the results suggest that MaBBX20 positively regulates light-induced anthocyanin biosynthesis in grape hyacinth, whereas MaBBX51 is a negative regulator.

Real-time regional risk prediction can play a crucial role in preventing traffic accidents. Thus, this study established a lane-level real-time regional risk prediction model. Based on observed data, the least squares-support vector machines (LS-SVM) algorithm was used to identify each lane region of the mainline, and the initial traffic parameters and surrogate safety measures (SSMs) were extracted and aggregated. The negative samples that characterized normal traffic and the positive samples that characterized regional risk were identified. Mutual information (MI) was used to determine the information gain of various feature variables in the samples, and the key feature variables affecting the regional conditions were tested and screened by means of binary logit regression analysis. Upon screening the variables and corresponding labels, the construction and verification of a lane-level regional risk prediction model was completed using the catastrophe theory. The results showed that lane difference is an important parameter to reduce the uncertainty of regional risk, and its odds ratio (OR) was 16.30 at the 95% confidence level. The 10%-quantile modified time to collision (MTTC) inverse, the speed difference between lanes, and 10%-quantile headway (DHW) had an obvious influence on regional status. The model achieved an overall accuracy of 86.50%, predicting 84.78% of regional risks with a false positive rate of 13.37% and 86.63% of normal traffic with a false positive rate of 15.22%. The proposed model can provide a basis for formulating individualized active traffic control strategies for different lanes.

Berberine is an isoquinoline alkaloid isolated from Chinese herbal medicines such as Coptis chinensis. It has many pharmacological actions, such as antibacterial, hypoglycemic, anti-inflammatory, and so on. However, due to the low lipophilicity of berberine, it is difficult to penetrate the bacterial cell membrane and also difficult to be absorbed orally and usually needs a relatively high dose to achieve the ideal effect. The purpose of this study is to transform the structure of berberine in order to improve the bioavailability of berberine and reduce the dosage. Moreover, we introduce a pharmacophore named Canagliflozin, a hypoglycemic drug (which was also found to have potential anti-bacterial activity) into BBR to see whether this new compound has more existed activities. We at first connected berberine with Canagliflozin, to form a new compound (BC) and see whether BC has synergic effects. We use microbroth dilution method to determine the minimum inhibitory concentration of BC, determine the bacterial growth with the enzyme labeling instrument, observe the formation of bacterial biofilm with crystal violet staining method, observe the bacterial morphology with field emission scanning electron microscope, and determine the intracellular protein with SDS-PAGE. The above indicators reflect the damage of BC to bacteria. New compound BC was successfully obtained by chemical synthesis. The minimal inhibitory concentration of compound BC on three bacteria was significantly better than that of berberine and canagliflozin alone and the combination of berberine and canagliflozin. Moreover, compound BC has obvious destructive effect on bacterial morphology and biofilm, and the compound also has destructive effect on intracellular proteins. Therefore, new compound BC has broad-spectrum antibacterial activity and the inhibitory effect of BC might play a role by destroying the integrity of biofilm and the intracellular protein of bacteria. In conclusion, we create a new molecular entity of berberine and Canagliflozin chimera and open up a new prospect for berberine derivatives in the treatment of bacterial infection.

Disposable paper cups are widely used in daily life and most of them are landfilled or incinerated after use, resulting in a serious ecological hazard and significant waste of resources due to the usage of thin polyethylene (PE) as their inner coating. Hence, converting these common solid domestic wastes into high-value added materials is attractive and meaningful. In this study, transparent cellulose-based films were achieved from old bamboo-based disposable paper cups after pretreatment through using the room ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl) as solvent. The cellulose-based film with a dense texture demonstrated a relatively nice mechanical and UV-shielding performances, and its tensile strength was as high as 48 MPa, much higher than that of commercial polyethylene (PE, 12 MPa) film. Thus, the resultant cellulose-based film showed a great potential in the packaging field. Besides, the flexible paper plastic composites (PPC) were also fabricated from the rest thin PE coating with the stuck fibers, and it was found that PPC showed excellent mechanical property and hydrophobicity. Consequently, a feasible and eco-friendly process of recycling and reusing waste disposable paper cups was developed to achieve a complete utilization and valorization of waste disposable paper cups.

Myogenic factor 5 plays active roles in the regulation of myogenesis. The aim of this study is to expose the genetic variants of the MYF5 and its association with growth performance and ultrasound traits in grassland short-tailed sheep (GSTS) in China. The combination technique of sequencing and SNaPshot revealed seven SNPs in ovine MYF5 from 533 adult individuals (male 103 and female 430), four of which are novel ones located at g.6838G > A, g.6989 G > T, g.7117 C > A in the promoter region and g.9471 T > G in the second intron, respectively. Genetic diversity indexes showed the seven SNPs in low or intermediate level, but each of them conformed HWE (p > 0.05) in genotypic frequencies. Association analysis indicated that g.6838G > A, g.7117 C > A, g.8371 T > C, g.9471 T > G, and g.10044 C > T had significant effects on growth performance and ultrasound traits. The diplotypes of H1H3 and H2H3 had higher body weight and greater body size, and haplotype H3 had better performance on meat production than the others. In addition, the dual-luciferase reporter assay showed that there are two active regions in the MYF5 promoter located at −1799~−1197 bp and −514~−241 bp, respectively, but g.6838G > A and g.7117 C > A were out of the region, suggesting these two SNPs influence the phenotype by other pathway. The results suggest that the MYF5 gene might be applied as a promising candidate of functional genetic marker in GSTS breeding.

cis -Splicing of adjacent genes ( cis -SAGe) has been involved in multiple physiological and pathological processes in humans. However, to the best of our knowledge, there is no report of cis -SAGe in adipogenic regulation. In this study, a cis -SAGe product, BCL2L2-PABPN1 (BP), was characterized in fat tissue of pigs with RT-PCR and RACE method. BP is an in-frame fusion product composed of 333 aa and all the functional domains of both parents. BP is highly conserved among species and rich in splicing variants. BP was found to promote proliferation and inhibit differentiation of primary porcine preadipocytes. A total of 3074/44 differentially expressed mRNAs (DEmRs)/known miRNAs (DEmiRs) were identified in porcine preadipocytes overexpressing BP through RNA-Seq analysis. Both DEmRs and target genes of DEmiRs were involved in various fat-related pathways with MAPK and PI3K-Akt being the top enriched. PPP2CB , EGFR , Wnt5A and EHHADH were hub genes among the fat-related pathways identified. Moreover, ssc-miR-339-3p was found to be critical for BP regulating adipogenesis through integrated analysis of mRNA and miRNA data. The results highlight the role of cis -SAGe in adipogenesis and contribute to further revealing the mechanisms underlying fat deposition, which will be conductive to human obesity control.

In this paper, considering the strength and geometric discrete distribution characteristics of composite reinforcement, by introducing the discrete distribution function of reinforcement, the secondary development of ABAQUS is realized by using the Python language, the parametric automatic generation method of representative volume elements of particle-reinforced composites is established, and the tensile properties of silicon carbide particle-reinforced aluminum matrix composites are analyzed. The effects of particle strength, particle volume fraction, and particle random distribution on the mechanical properties of SiCp/Al composites are studied. The results show that the random distribution of particles and the change in particle strength have no obvious influence on the stress-strain relationship before the beginning of material damage, but have a great influence on the damage stage, maximum strength, and corresponding failure strain. With the increase in particle volume fraction, the damage intensity of the model increases, and the random distribution of particles has a great influence on the model with a large particle volume fraction. The results can provide a reference for the design, preparation, and characterization of particle-reinforced metal matrix composites.

Meat quality is one of the most important economic traits in pig breeding and production. Intramuscular fat (IMF) is a major factor that improves meat quality. To better understand the alternative splicing (AS) events underlying meat quality, long-read isoform sequencing (Iso-seq) was used to identify differential (D)AS events between the longissimus thoracis (LT) and semitendinosus (ST), which differ in IMF content, together with short-read RNA-seq. Through Iso-seq analysis, we identified a total of 56,789 novel transcripts covering protein-coding genes, lncRNA, and fusion transcripts that were not previously annotated in pigs. We also identified 456,965 AS events, among which 3930 were DAS events, corresponding to 2364 unique genes. Through integrative analysis of Iso-seq and RNA-seq, we identified 1174 differentially expressed genes (DEGs), among which 122 were DAS genes, i.e., DE-DAS genes. There are 12 overlapped pathways between the top 20 DEGs and DE-DAS genes, as revealed by KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis, indicating that DE-DAS genes play important roles in the differential phenotype of LT and ST. Further analysis showed that upregulated DE-DAS genes are more important than downregulated ones in IMF deposition. Fatty acid degradation and the PPAR (peroxisome proliferator-activated receptor) signaling pathway were found to be the most important pathways regulating the differential fat deposition of the two muscles. The results update the existing porcine genome annotations and provide data for the in-depth exploration of the mechanisms underlying meat quality and IMF deposition.

Colitis is not fully curable, although currently, some treatment options are being adopted. In this study, we investigated the effects of pineapple leaf phenols (PLPs), natural phenol products from pineapple leaves, on DSS-induced colitis in mice. The results showed that PLPs dramatically decreased the inflammatory response by inhibiting NF-κB activation and the secretion of pro-inflammatory factors. Moreover, PLPs provided protection against DSS-induced acute colitis by maintaining epithelial integrity. Caffeic and P-coumaric acids had similar effects and could be the active components responsible for PLPs' effect on colitis. These results indicate that the oral administration of PLPs might be considered as a therapeutic strategy in the treatment of patients with colitis. However, further research on clinical applications and the exact effect of PLPs on colitis is required.

Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that instigates several signaling cascades, including the NF-κB signaling pathway, to induce cell differentiation and proliferation. Overexpression and mutations of EGFR are found in up to 30% of solid tumors and correlate with a poor prognosis. Although it is known that EGFR-mediated NF-κB activation is involved in tumor development, the signaling axis is not well elucidated. Here, we found that plakophilin 2 (PKP2) and the linear ubiquitin chain assembly complex (LUBAC) were required for EGFR-mediated NF-κB activation. Upon EGF stimulation, EGFR recruited PKP2 to the plasma membrane, and PKP2 bridged HOIP, the catalytic E3 ubiquitin ligase in the LUBAC, to the EGFR complex. The recruitment activated the LUBAC complex and the linear ubiquitination of NEMO, leading to IκB phosphorylation and subsequent NF-κB activation. Furthermore, EGF-induced linear ubiquitination was critical for tumor cell proliferation and tumor development. Knockout of HOIP impaired EGF-induced NF-κB activity and reduced cell proliferation. HOIP knockout also abrogated the growth of A431 epidermal xenograft tumors in nude mice by more than 70%. More importantly, the HOIP inhibitor, HOIPIN-8, inhibited EGFR-mediated NF-κB activation and cell proliferation of A431, MCF-7, and MDA-MB-231 cancer cells. Overall, our study reveals a novel linear ubiquitination signaling axis of EGFR and that perturbation of HOIP E3 ubiquitin ligase activity is potential targeted cancer therapy.

Self-healing concrete designs can protect against deterioration and improve durability. However, there is no unified conclusion regarding the effective preparation and mechanical properties of self-healing concrete. In this paper, microcapsules are used in cement-based materials, the reasonable dosage of microcapsules is determined, and the self-healing performance of the microcapsule self-healing system under different curing agents is explored. The microcapsules and curing agent are shown to enhance the flexural and compressive strength of mortar specimens at relatively low contents. The optimal microcapsule content in terms of compressive strength is 1-3%. When the content of the microcapsule reaches 7%, the strength of the specimen decreases by approximately 30%. Sodium fluorosilicate is better-suited to the microcapsule self-healing cement-based system than the other two fluorosilicates, potassium fluorosilicate and magnesium, which have similarly poor healing performance as curing agents. Healing time also appears to significantly influence the microcapsule self-healing system; mortar specimens that healed for 28 days are significantly higher than those that healed for 7 days. This work may provide a valuable reference for the design and preparation of self-healing cementitious composite structures.

Effective intracerebral delivery is key for glioma treatment. However, the drug delivery system within the brain is largely limited by its own adverse physical and chemical properties, low targeting efficiency, the blood-brain barrier and the blood-brain tumor barrier. Herein, we developed a simple, safe and efficient biomimetic nanosuspension. The C6 cell membrane (CCM) was utilized to camouflaged the 10-hydroxycamptothecin nanosuspension (HCPT-NS) in order to obtain HCPT-NS/CCM. Through the use of immune escape and homotypic binding of the cancer cell membrane, HCPT-NS/CCM was able to penetrate the blood-brain barrier and target tumors. The HCPT-NS is only comprised of drugs, as well as a small amount of stabilizers that are characterized by a simple preparation method and high drug loading. Similarly, the HCPT-NS/CCM is able to achieve targeted treatment of glioma without any ligand modification, which leads it to be stable and efficient. Cellular uptake and in vivo imaging experiments demonstrated that HCPT-NS/CCM is able to effectively cross the blood-brain barrier and was concentrated at the glioma site due to the natural homing pathway. Our results reveal that the glioma cancer cell membrane is able to promote drug transport into the brain and enter the tumor via a homologous targeting mechanism.

Recent achievements in the field of computer vision, reinforcement learning, and locomotion control have largely extended legged robots' maneuverability in complex natural environments. However, little research focuses on sensing and analyzing the physical properties of the ground, which is crucial to robots' locomotion during their interaction with highly irregular profiles, deformable terrains, and slippery surfaces. A biomimetic, flexible, multimodal sole sensor (FMSS) designed for legged robots to identify the ontological status and ground information, such as reaction force mapping, contact situation, terrain, and texture information, to achieve agile maneuvers was innovatively presented in this paper. The FMSS is flexible and large-loaded (20 Pa-800 kPa), designed by integrating a triboelectric sensing coat, embedded piezoelectric sensor, and piezoresistive sensor array. To evaluate the effectiveness and adaptability in different environments, the multimodal sensor was mounted on one of the quadruped robot's feet and one of the human feet then traversed through different environments in real-world tests. The experiment's results demonstrated that the FMSS could recognize terrain, texture, hardness, and contact conditions during locomotion effectively and retrain its sensitivity (0.66 kPa -1 ), robustness, and compliance. The presented work indicates the FMSS's potential to extend the feasibility and dexterity of tactile perception for state estimation and complex scenario detection.

The amounts of the components in a microcapsule self-healing system significantly impact the basic performance and self-healing performance of concrete. In this paper, an orthogonal experimental design is used to investigate the healing performance of microcapsule self-healing concrete under different pre-damage loads. The strength recovery performance and sound speed recovery performance under extensive damage are analyzed. The optimum factor combination of the microcapsule self-healing concrete is obtained. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) are carried out on the concrete samples before and after healing to determine the healing mechanism. The results show that the healing effect of self-healing concrete decreases with an increase in the pre-damage load, and the sound speed recovery rate increases with an increase in the damage degree. The influence of the sodium silicate content on the compressive strength and compressive strength recovery rate of the self-healing concrete increases, followed by a decrease. The optimum combination of factors of the microcapsule self-healing system is 3% microcapsules, 30% sodium silicate, and 15% sodium fluosilicate. The results can be used for the design and preparation of self-healing concrete.

As a popular marine saccharide, chitooligosaccharides (COS) has been proven to have good antioxidant activity. Its antioxidant effect is closely related to its degree of polymerization, degree of acetylation and sequence. However, the specific structure-activity relationship remains unclear. In this study, three chitosan dimers with different sequences were obtained by the separation and enzymatic method, and the antioxidant activity of all four chitosan dimers were studied. The effect of COS sequence on its antioxidant activity was revealed for the first time. The amino group at the reducing end plays a vital role in scavenging superoxide radicals and in the reducing power of the chitosan dimer. At the same time, we found that the fully deacetylated chitosan dimer DD showed the strongest DPPH scavenging activity. When the amino groups of the chitosan dimer were acetylated, it showed better activity in scavenging hydroxyl radicals. Research on COS sequences opens up a new path for the study of COS, and is more conducive to the investigation of its mechanism.

Rutin (R) and quercetin (Q) are two widespread dietary flavonoids. Previous studies regarding the plasma cholesterol-lowering activity of R and Q generated inconsistent results. The present study was therefore carried out to investigate the effects of R and Q on cholesterol metabolism in both HepG2 cells and hypercholesterolemia hamsters. Results from HepG2 cell experiments demonstrate that both R and Q decreased cholesterol at doses of 5 and 10 µM. R and Q up-regulated both the mRNA and protein expression of sterol regulatory element binding protein 2 (SREBP2), low-density lipoprotein receptor (LDLR), and liver X receptor alpha (LXRα). The immunofluorescence study revealed that R and Q increased the LDLR expression, while only Q improved LDL-C uptake in HepG2 cells. Results from hypercholesterolemia hamsters fed diets containing R (5.5 g/kg diet) and Q (2.5 g/kg diet) for 8 weeks demonstrate that both R and Q had no effect on plasma total cholesterol. In the liver, only Q reduced cholesterol significantly. The discrepancy between the in vitro and in vivo studies was probably due to a poor bioavailability of flavonoids in the intestine. It was therefore concluded that R and Q were effective in reducing cholesterol in HepG2 cells in vitro, whereas in vivo, the oral administration of the two flavonoids had little effect on plasma cholesterol in hamsters.