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Rutin and Quercetin Decrease Cholesterol in HepG2 Cells but Not Plasma Cholesterol in Hamsters by Oral Administration.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2021 Jun 21; Vol. 26 (12). Date of Electronic Publication: 2021 Jun 21. - Publication Year :
- 2021
-
Abstract
- Rutin (R) and quercetin (Q) are two widespread dietary flavonoids. Previous studies regarding the plasma cholesterol-lowering activity of R and Q generated inconsistent results. The present study was therefore carried out to investigate the effects of R and Q on cholesterol metabolism in both HepG2 cells and hypercholesterolemia hamsters. Results from HepG2 cell experiments demonstrate that both R and Q decreased cholesterol at doses of 5 and 10 µM. R and Q up-regulated both the mRNA and protein expression of sterol regulatory element binding protein 2 (SREBP2), low-density lipoprotein receptor (LDLR), and liver X receptor alpha (LXRα). The immunofluorescence study revealed that R and Q increased the LDLR expression, while only Q improved LDL-C uptake in HepG2 cells. Results from hypercholesterolemia hamsters fed diets containing R (5.5 g/kg diet) and Q (2.5 g/kg diet) for 8 weeks demonstrate that both R and Q had no effect on plasma total cholesterol. In the liver, only Q reduced cholesterol significantly. The discrepancy between the in vitro and in vivo studies was probably due to a poor bioavailability of flavonoids in the intestine. It was therefore concluded that R and Q were effective in reducing cholesterol in HepG2 cells in vitro, whereas in vivo, the oral administration of the two flavonoids had little effect on plasma cholesterol in hamsters.
- Subjects :
- Administration, Oral
Animals
Cell Line, Tumor
Cricetinae
Flavonoids pharmacology
Hep G2 Cells
Humans
Hypercholesterolemia blood
Hypercholesterolemia drug therapy
Hypercholesterolemia metabolism
Lipid Metabolism drug effects
Liver drug effects
Liver metabolism
Liver X Receptors metabolism
Male
RNA, Messenger metabolism
Receptors, LDL blood
Receptors, LDL metabolism
Sterol Regulatory Element Binding Protein 2 metabolism
Triglycerides blood
Triglycerides metabolism
Up-Regulation drug effects
Cholesterol blood
Cholesterol metabolism
Quercetin pharmacology
Rutin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 26
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 34205604
- Full Text :
- https://doi.org/10.3390/molecules26123766