1. Probiotic and Functional Properties of Limosilactobacillus reuteri INIA P572
- Author
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Margarita Medina, Patricia Diez-Echave, Arjan Narbad, Ana García-Lafuente, Melinda J. Mayer, José Garrido-Mesa, José María Landete, Julio Gálvez, Juan Luis Arqués, Laura Hidalgo-García, Teresa Vezza, Alba Rodríguez-Nogales, María Elena Rodríguez-Cabezas, Francesca Algieri, Izaskun Martín-Cabrejas, Susana Langa, [Diez-Echave,P, Garrido-Mesa,J, Vezza,T, Hidalgo-García,L, Algieri,F, Rodríguez-Nogales,A, Rodríguez-Cabezas,ME, Gálvez,J] Centro de Investigaciones Biomédicas en Red–Enfermedades Hepáticas y Digestivas (CIBER-EHD), Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, Granada, Spain. [Diez-Echave,P, Gálvez,J] Instituto de Investigación Biosanitaria de Granada (ibs.GRANADA), Granada, Spain. [Martín-Cabrejas,I, Langa,S, Landete,JM, Medina,M, Arqués,JL] Departamento Tecnología de Alimentos, INIA-CSIC, Madrid, Spain. [Mayer,MJ, Narbad,A] Gut Microbes and Health Institute Strategic Programme, Quadram Institute Bioscience, Norwich, UK. [García-Lafuente,A] Centro para la Calidad de los Alimentos, INIA-CISC, Soria, Spain., This work was supported by project no. RTA2017-00002-00-00 from the Spanish Ministry of Science and Innovation, by the Junta de Andalucía (CTS 164) and Instituto de Salud Carlos III (PI19/01058) with funds from the European Union., Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Instituto de Salud Carlos III, European Commission, Diez-Echave, Patricia (0000-0003-4493-3091), Martín-Cabrejas, Izaskun (0000-0002-7913-0291), Garrido-Mesa, José (0000-0001-6420-9055), Langa, Susana (0000-0003-2729-219X), Vezza, Teresa (0000-0002-9780-0180), Landete, José M (0000-0002-5147-3989), Hidalgo-García, Laura (0000-0002-5529-171X), Algieri, Francesca (0000-0002-0262-4867), Mayer, Melinda J (0000-0002-8764-2836), Narbad, Arjan (0000-0003-2968-7558), García-Lafuente, Ana (0000-0002-5147-3145), Medina, Margarita (0000-0001-9692-4977), Rodríguez-Nogales, Alba (0000-0003-1927-0628), Rodríguez-Cabezas, María Elena (0000-0001-9085-8147), Gálvez, Julio (0000-0001-6876-3782), and Arqués, Juan L (0000-0002-8548-0183)
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Male ,0301 basic medicine ,Limosilactobacillus reuteri ,Colonic model ,Probiotic ,Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus::Lactobacillus reuteri [Medical Subject Headings] ,law.invention ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] ,Mice ,Anatomy::Cells::Cells, Cultured::Cell Line [Medical Subject Headings] ,law ,Inmunomodulación ,Chemicals and Drugs::Organic Chemicals::Aldehydes::Glyceraldehyde [Medical Subject Headings] ,Organisms::Eukaryota::Animals [Medical Subject Headings] ,Technology and Food and Beverages::Food and Beverages::Food::Dairy Products [Medical Subject Headings] ,TX341-641 ,Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons, Acyclic::Alkanes::Propane [Medical Subject Headings] ,Chemicals and Drugs::Carbohydrates::Polysaccharides::Glucans::Dextrans::Dextran Sulfate [Medical Subject Headings] ,Nutrition and Dietetics ,Strain (chemistry) ,In vitro toxicology ,Antimicrobial compound ,Reuterin ,Anatomy::Cells::Myeloid Cells::Macrophages [Medical Subject Headings] ,Sulfato de Dextran ,Línea Celular ,Technology and Food and Beverages::Food and Beverages::Food::Dietary Supplements::Probiotics [Medical Subject Headings] ,Sodium ,030106 microbiology ,Lactobacillus reuteri ,chemistry.chemical_element ,Check Tags::Male [Medical Subject Headings] ,In Vitro Techniques ,Protectores contra radiación ,Glyceraldehyde ,Microbiology ,Protective effect ,Immunomodulation ,Propane ,03 medical and health sciences ,In vivo ,Animals ,Humans ,Macrófagos ,Inflammation ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings] ,Nutrition. Foods and food supply ,Macrophages ,Probiotics ,Probióticos ,Dextran sulphate sodium ,In vitro ,Diseases::Animal Diseases::Disease Models, Animal [Medical Subject Headings] ,Gastrointestinal Tract ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,chemistry ,Dextran sulphate ,DSS-induced colitic model ,Anatomy::Digestive System::Gastrointestinal Tract [Medical Subject Headings] ,Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Laboratory::Animals, Inbred Strains::Mice, Inbred Strains::Mice, Inbred C57BL [Medical Subject Headings] ,Cell line ,Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Inflammation [Medical Subject Headings] ,Food Science - Abstract
18 Pág. Departamento de Tecnología de Alimentos, Limosilactobacillus reuteri INIA P572 is a strain able to produce the antimicrobial compound reuterin in dairy products, exhibiting a protective effect against some food-borne pathogens. In this study, we investigated some probiotic properties of this strain such as resistance to gastrointestinal passage or to colonic conditions, reuterin production in a colonic environment, and immunomodulatory activity, using different in vitro and in vivo models. The results showed a high resistance of this strain to gastrointestinal conditions, as well as capacity to grow and produce reuterin in a human colonic model. Although the in vitro assays using the RAW 264.7 macrophage cell line did not demonstrate direct immunomodulatory properties, the in vivo assays using a Dextran Sulphate Sodium (DSS)-induced colitic mice model showed clear immunomodulatory and protective effects of this strain., This work was supported by project no. RTA2017-00002-00-00 from the Spanish Ministry of Science and Innovation, by the Junta de Andalucía (CTS 164) and Instituto de Salud Carlos III (PI19/01058) with funds from the European Union
- Published
- 2021
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