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Probiotic and Functional Properties of Limosilactobacillus reuteri INIA P572

Authors :
Margarita Medina
Patricia Diez-Echave
Arjan Narbad
Ana García-Lafuente
Melinda J. Mayer
José Garrido-Mesa
José María Landete
Julio Gálvez
Juan Luis Arqués
Laura Hidalgo-García
Teresa Vezza
Alba Rodríguez-Nogales
María Elena Rodríguez-Cabezas
Francesca Algieri
Izaskun Martín-Cabrejas
Susana Langa
[Diez-Echave,P
Garrido-Mesa,J
Vezza,T
Hidalgo-García,L
Algieri,F
Rodríguez-Nogales,A
Rodríguez-Cabezas,ME
Gálvez,J] Centro de Investigaciones Biomédicas en Red–Enfermedades Hepáticas y Digestivas (CIBER-EHD), Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, Granada, Spain. [Diez-Echave,P
Gálvez,J] Instituto de Investigación Biosanitaria de Granada (ibs.GRANADA), Granada, Spain. [Martín-Cabrejas,I
Langa,S
Landete,JM
Medina,M
Arqués,JL] Departamento Tecnología de Alimentos, INIA-CSIC, Madrid, Spain. [Mayer,MJ
Narbad,A] Gut Microbes and Health Institute Strategic Programme, Quadram Institute Bioscience, Norwich, UK. [García-Lafuente,A] Centro para la Calidad de los Alimentos, INIA-CISC, Soria, Spain.
This work was supported by project no. RTA2017-00002-00-00 from the Spanish Ministry of Science and Innovation, by the Junta de Andalucía (CTS 164) and Instituto de Salud Carlos III (PI19/01058) with funds from the European Union.
Ministerio de Ciencia e Innovación (España)
Junta de Andalucía
Instituto de Salud Carlos III
European Commission
Diez-Echave, Patricia (0000-0003-4493-3091)
Martín-Cabrejas, Izaskun (0000-0002-7913-0291)
Garrido-Mesa, José (0000-0001-6420-9055)
Langa, Susana (0000-0003-2729-219X)
Vezza, Teresa (0000-0002-9780-0180)
Landete, José M (0000-0002-5147-3989)
Hidalgo-García, Laura (0000-0002-5529-171X)
Algieri, Francesca (0000-0002-0262-4867)
Mayer, Melinda J (0000-0002-8764-2836)
Narbad, Arjan (0000-0003-2968-7558)
García-Lafuente, Ana (0000-0002-5147-3145)
Medina, Margarita (0000-0001-9692-4977)
Rodríguez-Nogales, Alba (0000-0003-1927-0628)
Rodríguez-Cabezas, María Elena (0000-0001-9085-8147)
Gálvez, Julio (0000-0001-6876-3782)
Arqués, Juan L (0000-0002-8548-0183)
Source :
Digibug: Repositorio Institucional de la Universidad de Granada, Universidad de Granada (UGR), Nutrients, Vol 13, Iss 1860, p 1860 (2021), Nutrients, Volume 13, Issue 6, Digibug. Repositorio Institucional de la Universidad de Granada, instname
Publication Year :
2021
Publisher :
MDPI, 2021.

Abstract

18 Pág. Departamento de Tecnología de Alimentos​​<br />Limosilactobacillus reuteri INIA P572 is a strain able to produce the antimicrobial compound reuterin in dairy products, exhibiting a protective effect against some food-borne pathogens. In this study, we investigated some probiotic properties of this strain such as resistance to gastrointestinal passage or to colonic conditions, reuterin production in a colonic environment, and immunomodulatory activity, using different in vitro and in vivo models. The results showed a high resistance of this strain to gastrointestinal conditions, as well as capacity to grow and produce reuterin in a human colonic model. Although the in vitro assays using the RAW 264.7 macrophage cell line did not demonstrate direct immunomodulatory properties, the in vivo assays using a Dextran Sulphate Sodium (DSS)-induced colitic mice model showed clear immunomodulatory and protective effects of this strain.<br />This work was supported by project no. RTA2017-00002-00-00 from the Spanish Ministry of Science and Innovation, by the Junta de Andalucía (CTS 164) and Instituto de Salud Carlos III (PI19/01058) with funds from the European Union

Subjects

Subjects :
Male
0301 basic medicine
Limosilactobacillus reuteri
Colonic model
Probiotic
Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus::Lactobacillus reuteri [Medical Subject Headings]
law.invention
Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]
Mice
Anatomy::Cells::Cells, Cultured::Cell Line [Medical Subject Headings]
law
Inmunomodulación
Chemicals and Drugs::Organic Chemicals::Aldehydes::Glyceraldehyde [Medical Subject Headings]
Organisms::Eukaryota::Animals [Medical Subject Headings]
Technology and Food and Beverages::Food and Beverages::Food::Dairy Products [Medical Subject Headings]
TX341-641
Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons, Acyclic::Alkanes::Propane [Medical Subject Headings]
Chemicals and Drugs::Carbohydrates::Polysaccharides::Glucans::Dextrans::Dextran Sulfate [Medical Subject Headings]
Nutrition and Dietetics
Strain (chemistry)
In vitro toxicology
Antimicrobial compound
Reuterin
Anatomy::Cells::Myeloid Cells::Macrophages [Medical Subject Headings]
Sulfato de Dextran
Línea Celular
Technology and Food and Beverages::Food and Beverages::Food::Dietary Supplements::Probiotics [Medical Subject Headings]
Sodium
030106 microbiology
Lactobacillus reuteri
chemistry.chemical_element
Check Tags::Male [Medical Subject Headings]
In Vitro Techniques
Protectores contra radiación
Glyceraldehyde
Microbiology
Protective effect
Immunomodulation
Propane
03 medical and health sciences
In vivo
Animals
Humans
Macrófagos
Inflammation
Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings]
Nutrition. Foods and food supply
Macrophages
Probiotics
Probióticos
Dextran sulphate sodium
In vitro
Diseases::Animal Diseases::Disease Models, Animal [Medical Subject Headings]
Gastrointestinal Tract
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
chemistry
Dextran sulphate
DSS-induced colitic model
Anatomy::Digestive System::Gastrointestinal Tract [Medical Subject Headings]
Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Laboratory::Animals, Inbred Strains::Mice, Inbred Strains::Mice, Inbred C57BL [Medical Subject Headings]
Cell line
Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Inflammation [Medical Subject Headings]
Food Science

Details

Language :
English
Database :
OpenAIRE
Journal :
Digibug: Repositorio Institucional de la Universidad de Granada, Universidad de Granada (UGR), Nutrients, Vol 13, Iss 1860, p 1860 (2021), Nutrients, Volume 13, Issue 6, Digibug. Repositorio Institucional de la Universidad de Granada, instname
Accession number :
edsair.doi.dedup.....c896840c30addeb039ace2f2ff7906f8
Full Text :
https://doi.org/10.3390/nu13061860