1. Assessment of Photodynamic Inactivation against Periodontal Bacteria Mediated by a Chitosan Hydrogel in a 3D Gingival Model.
- Author
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Peng PC, Hsieh CM, Chen CP, Tsai T, and Chen CT
- Subjects
- Aggregatibacter actinomycetemcomitans drug effects, Aggregatibacter actinomycetemcomitans physiology, Aggregatibacter actinomycetemcomitans radiation effects, Bacterial Infections drug therapy, Bacterial Infections microbiology, Biofilms drug effects, Biofilms radiation effects, Dose-Response Relationship, Radiation, Gingiva drug effects, Gingiva microbiology, Gingiva radiation effects, Gingival Diseases microbiology, Humans, Hydrogel, Polyethylene Glycol Dimethacrylate chemistry, Hypromellose Derivatives chemistry, Hypromellose Derivatives therapeutic use, Light, Models, Anatomic, Periodontal Pocket microbiology, Photosensitizing Agents chemistry, Photosensitizing Agents therapeutic use, Porphyromonas gingivalis drug effects, Porphyromonas gingivalis physiology, Porphyromonas gingivalis radiation effects, Staphylococcus aureus drug effects, Staphylococcus aureus physiology, Staphylococcus aureus radiation effects, Tolonium Chloride chemistry, Tolonium Chloride therapeutic use, Treatment Outcome, Chitosan chemistry, Gingival Diseases drug therapy, Hydrogel, Polyethylene Glycol Dimethacrylate therapeutic use, Photochemotherapy methods
- Abstract
Chitosan hydrogels containing hydroxypropyl methylcellulose (HPMC) and toluidine blue O were prepared and assessed for their mucoadhesive property and antimicrobial efficacy of photodynamic inactivation (PDI). Increased HPMC content in the hydrogels resulted in increased mucoadhesiveness. Furthermore, we developed a simple In Vitro 3D gingival model resembling the oral periodontal pocket to culture the biofilms of Staphylococcus aureus ( S. aureus ), Aggregatibacter actinomycetemcomitans ( A. actinomycetemcomitans ), and Porphyromonas gingivalis ( P. gingivalis ). The PDI efficacy of chitosan hydrogel was examined against periodontal biofilms cultured in this 3D gingival model. We found that the PDI effectiveness was limited due to leaving some of the innermost bacteria alive at the non-illuminated site. Using this 3D gingival model, we further optimized PDI procedures with various adjustments of light energy and irradiation sites. The PDI efficacy of the chitosan hydrogel against periodontal biofilms can significantly improve via four sides of irradiation. In conclusion, this study not only showed the clinical applicability of this chitosan hydrogel but also the importance of the light irradiation pattern in performing PDI for periodontal disease., Competing Interests: The authors declare no conflict of interest.
- Published
- 2016
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