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Assessment of Photodynamic Inactivation against Periodontal Bacteria Mediated by a Chitosan Hydrogel in a 3D Gingival Model.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2016 Nov 01; Vol. 17 (11). Date of Electronic Publication: 2016 Nov 01. - Publication Year :
- 2016
-
Abstract
- Chitosan hydrogels containing hydroxypropyl methylcellulose (HPMC) and toluidine blue O were prepared and assessed for their mucoadhesive property and antimicrobial efficacy of photodynamic inactivation (PDI). Increased HPMC content in the hydrogels resulted in increased mucoadhesiveness. Furthermore, we developed a simple In Vitro 3D gingival model resembling the oral periodontal pocket to culture the biofilms of Staphylococcus aureus ( S. aureus ), Aggregatibacter actinomycetemcomitans ( A. actinomycetemcomitans ), and Porphyromonas gingivalis ( P. gingivalis ). The PDI efficacy of chitosan hydrogel was examined against periodontal biofilms cultured in this 3D gingival model. We found that the PDI effectiveness was limited due to leaving some of the innermost bacteria alive at the non-illuminated site. Using this 3D gingival model, we further optimized PDI procedures with various adjustments of light energy and irradiation sites. The PDI efficacy of the chitosan hydrogel against periodontal biofilms can significantly improve via four sides of irradiation. In conclusion, this study not only showed the clinical applicability of this chitosan hydrogel but also the importance of the light irradiation pattern in performing PDI for periodontal disease.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Aggregatibacter actinomycetemcomitans drug effects
Aggregatibacter actinomycetemcomitans physiology
Aggregatibacter actinomycetemcomitans radiation effects
Bacterial Infections drug therapy
Bacterial Infections microbiology
Biofilms drug effects
Biofilms radiation effects
Dose-Response Relationship, Radiation
Gingiva drug effects
Gingiva microbiology
Gingiva radiation effects
Gingival Diseases microbiology
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate chemistry
Hypromellose Derivatives chemistry
Hypromellose Derivatives therapeutic use
Light
Models, Anatomic
Periodontal Pocket microbiology
Photosensitizing Agents chemistry
Photosensitizing Agents therapeutic use
Porphyromonas gingivalis drug effects
Porphyromonas gingivalis physiology
Porphyromonas gingivalis radiation effects
Staphylococcus aureus drug effects
Staphylococcus aureus physiology
Staphylococcus aureus radiation effects
Tolonium Chloride chemistry
Tolonium Chloride therapeutic use
Treatment Outcome
Chitosan chemistry
Gingival Diseases drug therapy
Hydrogel, Polyethylene Glycol Dimethacrylate therapeutic use
Photochemotherapy methods
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 17
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 27809278
- Full Text :
- https://doi.org/10.3390/ijms17111821