1,056 results on '"Hurtado A"'
Search Results
2. Spatiotemporal analysis of malaria transmission in the autonomous Indigenous regions of Panama, Central America, 2015-2022
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Cumbrera, Alberto, Calzada, Jose Eduardo, Chaves, Luis Fernando, and Hurtado, Lisbeth Amarilis
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- 2024
3. Bioactive Compounds in Rosehip (Rosa canina) Powder with Encapsulating Agents
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Igual, Marta, García Herrera, Patricia, Cámara Hurtado, Rosa María, Martínez-Monzó, Javier, García-Segovia, Purificación, Cámara Hurtado, María De La Montaña, Igual, Marta, García Herrera, Patricia, Cámara Hurtado, Rosa María, Martínez-Monzó, Javier, García-Segovia, Purificación, and Cámara Hurtado, María De La Montaña
- Abstract
Rosa canina pseudo-fruits contain interesting bioactive compounds. This work aims to evaluate the use of different biopolymers as encapsulating agents on the content of organic acids, minerals, fibers, phenols, carotenoids, and the antioxidant activity of the powdered product. Fruits were ground and freeze-dried with or without biopolymers (maltodextrin, resistant maltodextrin, cyclodextrin, and pea protein). Rosehip formulated purees with encapsulating agents are an interesting food ingredient rich in fiber and minerals that could be used in the food industry in order to obtain different functional foods. Results obtained in this study show that all formulated samples are a good source of potassium, calcium, magnesium, and manganese. Both rosehip without biopolymers and rosehip with pea protein formulations are also a good source of Zn. Formulation with pea protein can be claimed as a good source of Fe. All formulations are food ingredients with a very high content of ascorbic acid. Comparing the encapsulating agents, depending on the studied bioactive compound samples behaved differently. In conclusion, it can be indicated that pea protein is recommended as an encapsulating agent since the rosehip with pea protein sample has the highest content of fiber, minerals, organic acids, and carotenoids among the encapsulating agents studied., UPV-CUINA, UCM-ALIMNOVA Research Group, Depto. de Nutrición y Ciencia de los Alimentos, Fac. de Farmacia, TRUE, pub
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- 2024
4. In-Plane Liftout and Push-to-Pull for In Situ Mechanical Testing of Irradiated Inconel X-750.
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Gomez-Hurtado, Lucia R., Yao, Tiankai, Teng, Fei, Matos II, Mario D., Hawkins, Laura, Yang, Ge, and Wang, Yachun
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FOCUSED ion beams , *INCONEL , *TENSILE strength , *TRANSMISSION electron microscopy , *ELECTRON diffraction - Abstract
A streamlined sample preparation method for nanomechanical testing is needed to improve the quality of specimens, reduce the cost, and increase the versatility of specimen fabrication. This work outlines an in-plane liftout focused ion beam (FIB) fabrication procedure to prepare electron-transparent specimens for in situ transmission electron microscopy (TEM) nanomechanical testing. Ion etching and electron backscatter diffraction (EBSD) techniques were used to lift out a [110] oriented grain from a neutron-irradiated bulk X-750 alloy. Careful control of voltages and currents ensured precision. Top surface thinning sweeps prevented resurfacing and redeposition while dog-bone geometries were shaped with a 1:4 gauge width-to-milling pattern diameter ratio. Nanotensile testing in the TEM with a picoindenter allowed for the estimation of an ultimate tensile strength of 2.41 GPa, and inspection revealed a high density of bubbles in the X-750 matrix. The proposed fabrication procedure is significant for preparing samples from radioactive materials, studying complex structures that are orientation-dependent, and analyzing desired planar areas. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru.
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Quiñones-Cerna, Claudio, Castañeda-Aspajo, Alina, Tirado-Gutierrez, Marycielo, Salirrosas-Fernández, David, Rodríguez-Soto, Juan Carlos, Cruz-Monzón, José Alfredo, Hurtado-Butrón, Fernando, Ugarte-López, Wilmer, Gutiérrez-Araujo, Mayra, Quezada-Alvarez, Medardo Alberto, Gálvez-Rivera, Julieta Alessandra, and Esparza-Mantilla, Mario
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SOIL remediation ,HAZARDOUS waste sites ,AGRICULTURE ,SOIL solutions ,PETROLEUM - Abstract
Pollution from crude oil and its derivatives poses a serious threat to human health and ecosystems, with accidental spills causing substantial damage. Biodegradation, using microorganisms to break down these contaminants, presents a promising and cost-effective solution. Exploring and utilizing new bacterial strains from underexplored habitats could improve remediation efforts at contaminated sites. This study aimed to evaluate the hydrocarbon biodegradation capacity of bacteria isolated from agricultural soils in Huamachuco, Peru. Soil samples from Oca crops were collected and bacteria were isolated. Biodegradation assays were conducted using diesel as the sole carbon source in the Bushnell Haas Mineral medium. Molecular characterization of the 16S rRNA gene identified four strains. Diesel biodegradation assays at 1% concentration were performed under agitation conditions at 150 rpm and 30 °C, and monitored on day 10 by measuring cellular biomass (OD
600 ), with hydrocarbons analyzed by gas chromatography. The results showed Pseudomonas protegens (PROM2) achieved the highest efficiency in removing total hydrocarbons (91.5 ± 0.7%). Additionally, Pseudomonas citri PROM3 and Acinetobacter guillouiae ClyRoM5 also demonstrated high capacity in removing several individual hydrocarbons. Indigenous bacteria from uncontaminated agricultural soils present a high potential for hydrocarbon bioremediation, offering an ecological and effective solution for soil decontamination. [ABSTRACT FROM AUTHOR]- Published
- 2024
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6. Novel Insights on Extracellular Electron Transfer Networks in the Desulfovibrionaceae Family: Unveiling the Potential Significance of Horizontal Gene Transfer.
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Gonzalez, Valentina, Abarca-Hurtado, Josefina, Arancibia, Alejandra, Claverías, Fernanda, Guevara, Miguel R., and Orellana, Roberto
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HORIZONTAL gene transfer ,MOBILE genetic elements ,SULFATE-reducing bacteria ,CHARGE exchange ,MEMBRANE proteins - Abstract
Some sulfate-reducing bacteria (SRB), mainly belonging to the Desulfovibrionaceae family, have evolved the capability to conserve energy through microbial extracellular electron transfer (EET), suggesting that this process may be more widespread than previously believed. While previous evidence has shown that mobile genetic elements drive the plasticity and evolution of SRB and iron-reducing bacteria (FeRB), few have investigated the shared molecular mechanisms related to EET. To address this, we analyzed the prevalence and abundance of EET elements and how they contributed to their differentiation among 42 members of the Desulfovibrionaceae family and 23 and 59 members of Geobacteraceae and Shewanellaceae, respectively. Proteins involved in EET, such as the cytochromes PpcA and CymA, the outer membrane protein OmpJ, and the iron–sulfur cluster-binding CbcT, exhibited widespread distribution within Desulfovibrionaceae. Some of these showed modular diversification. Additional evidence revealed that horizontal gene transfer was involved in the acquiring and losing of critical genes, increasing the diversification and plasticity between the three families. The results suggest that specific EET genes were widely disseminated through horizontal transfer, where some changes reflected environmental adaptations. These findings enhance our comprehension of the evolution and distribution of proteins involved in EET processes, shedding light on their role in iron and sulfur biogeochemical cycling. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Leveraging Gamification in ICT Education: Examining Gender Differences and Learning Outcomes in Programming Courses.
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Mellado, Rafael, Cubillos, Claudio, Vicari, Rosa Maria, and Gasca-Hurtado, Gloria
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GENDER differences in education ,ACADEMIC motivation ,GENDER differences (Psychology) ,STUDENT engagement ,TECHNOLOGY Acceptance Model - Abstract
This study investigates the differential effects of gamification on learning outcomes, motivation, and usability perceptions in an introductory programming course, focusing on gender differences. While gamification has shown promise for increasing student engagement in educational settings, its impact may vary across genders. An experimental study was conducted with 88 university students randomly assigned to gamified and non-gamified groups. Learning gains were assessed through pre- and post-tests, motivational factors were measured via questionnaires, and usability perceptions were evaluated using the Technology Acceptance Model (TAM) questionnaire. Results revealed that women learned significantly more than men in the non-gamified condition, while men outperformed women in the gamified condition. Furthermore, men reported higher enjoyment, usefulness, and comfort with the gamified tool than women. Interestingly, both genders indicated greater satisfaction with the non-gamified version. These findings contribute nuanced insights into how gamification impacts genders differently in programming education, suggesting that gamification may hinder women's learning while modestly benefiting men. The study highlights the importance for practitioners to carefully consider gender dynamics when implementing gamified approaches, potentially offering customization options or blended techniques to optimize learning outcomes for all students in programming education. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Spermiogram, Kinetics, Flow Cytometric Characteristics and DNA Damage Degree in Boar Ejaculates: Summarization and Clustering.
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Ausejo-Marcos, Raquel, Tejedor, María Teresa, Miguel-Jiménez, Sara, Gómez-Giménez, Belén, Soriano-Úbeda, Cristina, Mendoza, Noelia, Vicente-Carrillo, Alejandro, Hurtado, William Fernando, Ávila Holguín, Celia, Moreno, Bernardino, and Falceto, María Victoria
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SEMEN analysis ,CATHEPSIN B ,ACROSOME reaction ,DNA damage ,SPERM motility ,ADP-ribosylation - Abstract
Simple Summary: Artificial insemination is nowadays a widespread practice; boar semen analysis is an essential key to guaranteeing pregnancy and high prolificacy. This analysis can include sperm motility, concentration, morphology, membrane integrity, DNA damage and seminal plasma components; a huge amount of data is available for each ejaculate and the interpretation of these results is complicated. This study aims to summarize these data, to classify ejaculates in several categories (clusters) and to investigate the potential differences among clusters on fertility and prolificacy. Two groups of Pietrain boars were studied (90 and 30 ejaculates weekly in groups 1 and 2, respectively). Computer-assisted semen analysis (CASA) and flow cytometry analysis were performed, and statistical analysis of the results was carried out using SPSS v.26 software. In both groups, variables were grouped in three principal components related to sperm velocity, linearity and DNA damage that allowed the ejaculates to be grouped into four clusters. Although an adequate description of the characteristics of the ejaculates was achieved, no differences were found among clusters for fertility or prolificacy since, with efficient quality control of semen, no relationships with fertility parameters would be expected once the minimum requirements have been met. Boar semen analysis includes sperm motility, concentration, morphology and other more complex analyses such as membrane integrity, DNA damage and seminal plasma components. This study aims to summarize these numerous data by linear combinations of them, to classify ejaculates in several categories (clusters) and to investigate the potential differences among clusters on fertility and prolificacy. Young Pietrain boars (23 ± 3.6 months) were investigated: ten boars from the Nucléus genetic line (group 1: 90 ejaculates weekly) and five boars from the Batallé genetic line (group 2: 30 ejaculates weekly). Computer-assisted semen analysis (CASA) examined motility. Sperm viability, acrosome reaction, early apoptosis, mitochondrial activity and DNA damage were studied by flow cytometry analysis. SPSS v.26 software was used to perform principal component analysis (PCA) and clustering. Three principal components (PC1: speed; PC2: linear path; PC3: DNA damage) were detected and four clusters identified in both groups. Clusters also differed significantly in several variables not included in these PCs (group 1: beat cross frequency and poly (ADP-ribose) polymerase; group 2: cathepsin B, abnormal forms, mitochondrial activity and high DNA stainability). PCA and clustering achieved adequate description of these ejaculates, but no differences among clusters were found for fertility or prolificacy, probably because the minimum sperm requirements had been met. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Enhancing Fatigue Resistance of Polylactic Acid through Natural Reinforcement in Material Extrusion.
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Bermudo Gamboa, Carolina, Martín-Béjar, Sergio, Bañón García, Fermín, and Sevilla Hurtado, Lorenzo
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FATIGUE limit ,FATIGUE life ,POLYLACTIC acid ,CELLULOSE fibers ,SYNTHETIC fibers ,NATURAL fibers - Abstract
This research paper aims to enhance the fatigue resistance of polylactic acid (PLA) in Material Extrusion (ME) by incorporating natural reinforcement, focusing on rotational bending fatigue. The study investigates the fatigue behavior of PLA in ME, using various natural fibers such as cellulose, coffee, and flax as potential reinforcements. It explores the optimization of printing parameters to address challenges like warping and shrinkage, which can affect dimensional accuracy and fatigue performance, particularly under the rotational bending conditions analyzed. Cellulose emerges as the most promising natural fiber reinforcement for PLA in ME, exhibiting superior resistance to warping and shrinkage. It also demonstrates minimal geometrical deviations, enabling the production of components with tighter dimensional tolerances. Additionally, the study highlights the significant influence of natural fiber reinforcement on the dimensional deviations and rotational fatigue behavior of printed components. The fatigue resistance of PLA was significantly improved with natural fiber reinforcements. Specifically, PLA reinforced with cellulose showed an increase in fatigue life, achieving up to 13.7 MPa stress at 70,000 cycles compared to unreinforced PLA. PLA with coffee and flax fibers also demonstrated enhanced performance, with stress values reaching 13.6 MPa and 13.5 MPa, respectively, at similar cycle counts. These results suggest that natural fiber reinforcements can effectively improve the fatigue resistance and dimensional stability of PLA components produced by ME. This paper contributes to the advancement of additive manufacturing by introducing natural fiber reinforcement as a sustainable solution to enhance PLA performance under rotational bending fatigue conditions. It offers insights into the comparative effectiveness of natural fibers and synthetic counterparts, particularly emphasizing the superior performance of cellulose. [ABSTRACT FROM AUTHOR]
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- 2024
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10. What Women with HIV Know about Heart Health and Cardiovascular Risk and Intervention Preferences.
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Duthely, Lunthita M., Satish, Sanjana, Kedia, Sapna A., Vilchez, Lilliana, Valls, Priscilla T., Larson, Michaela E., O'Reilly, Carolina Cruzval, Hurtado, Vanessa, Bernal, Maria Camila, Inestroza, Karla, Nogueira, Nicholas F., Glynn, Tiffany R., Kanamori, Mariano J., and Martinez, Claudia A.
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- 2024
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11. The Design of a Strategic Platform for the Smart Supervision of Public Expenditure for Colombia in the Context of Society 5.0.
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Restrepo-Carmona, Jaime A., Zuluaga, Juan C., Flórez, Diego A., Gómez, Mario S., Londoño, Laura, Gómez, Gabriela, Villamil, Rosse M., Morales, Olguer, Hurtado, Ángela M., Escobar, Carlos A., Sierra-Pérez, Julián, and Vásquez, Rafael E.
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PUBLIC spending ,DIGITAL transformation ,QUALITY of life ,LITERATURE reviews ,DATA analytics - Abstract
The overarching vision of Society 5.0 seeks to integrate technology to enhance quality of life and address social issues, with the primary goal of creating human-centered communities, which nowadays represent the inhabitants of smart cities. In this context, this work addresses the design of a modular strategic platform for the smart supervision of public expenditure, to be used by the Directorate of Information, Analysis, and Immediate Reaction (DIARI) of the General Comptroller of the Republic (CGR) of Colombia as a significant contribution towards the country's transition into Society 5.0. The design was performed by conducting a detailed literature review on fiscal control; performing a comprehensive analysis of the legal, organizational, and technological aspects of the country and the CGR; and developing six functional modules focused on topics such as continuous learning, strategic decision making, distinctive value, strategic achievements, capacity building, and organization, within a three-year implementation plan, through a participatory approach. This plan aims to integrate the platform to enable real-time monitoring, early detection of irregularities, and increased transparency in the management of data and public resources; since the start of the operation of the strategic platform in 2024, the DIARI increased the number of alerts generated by 29% over a three-month period with respect to the year 2023. The strategic platform for the DIARI of the CGR is useful for smart cities and the transition into Society 5.0 since it ensures efficient public expenditure management, enhancing transparency and citizen empowerment through modern technologies, data analytics, and active participation in governance processes. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Handgrip Strength and Upper Limb Anthropometric Characteristics among Latin American Female Volleyball Players.
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Camacho-Villa, María Alejandra, Hurtado-Alcoser, Jhon, Jerez, Andrés Santiago, Saavedra, Juan Carlos, Paredes Prada, Erika Tatiana, Merchán, Jeimy Andrea, Millan-Domingo, Fernando, Silva-Polanía, Carlos, and De la Rosa, Adrián
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VOLLEYBALL players ,GRIP strength ,BODY mass index ,WOMEN athletes ,PREVENTION of injury ,WRIST - Abstract
Background: In volleyball, the upper limb dimensions and grip strength greatly influence offensive and defensive movements during a match. However, the relationship between these parameters remains underexplored in elite female volleyball players. Objective: This study aimed to contrast the upper limb anthropometric characteristics and handgrip strength (HGS) of female elite volleyball players against a control group. Methods: Selected upper limb anthropometric parameters and maximal HGS of 42 female volleyball players and 40 non-athletes were measured. Results: Players exhibited higher values in almost all variables studied than non-athletes. The differences were statistically significant (p < 0.001) except for body mass index and elbow and wrist diameters. Players showed a moderate correlation between dominant HGS and hand parameters (length r = 0.43 and breadth r = 0.63; p < 0.05). Weak correlations were identified with height, upper arm length, elbow diameter, and hand shape index (r = 0.32 to 0.38; p < 0.05). In the non-dominant hand, a moderate correlation with handbreadth (r = 0.55, p ≤ 0.01) and weak correlations with upper arm length, wrist diameter, hand length, and hand shape index (r = 0.32 to 0.35; p ≤ 0.05) was found. Conclusions: These findings underscore the importance of the upper limb anthropometric parameters as predictors of HGS and their utility in athlete selection. Future research should investigate biomechanical factors influencing HGS and injury prevention. [ABSTRACT FROM AUTHOR]
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- 2024
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13. Study of a New Novel HVOAF Coating Based on a New Multicomponent Al80Mg10Si5Cu5 Alloy.
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Villanueva, Ester, Vicario, Iban, Vaquero, Carlos, Albizuri, Joseba, Guraya, Maria Teresa, Burgos, Nerea, and Hurtado, Iñaki
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ENERGY dispersive X-ray spectroscopy ,PLASMA spraying ,ELECTRIC conductivity ,MECHANICAL wear ,SCANNING electron microscopy ,METAL spraying - Abstract
This paper presents and demonstrates the development of a new lightweight coating for aluminum alloy from a novel multicomponent alloy based on the AlSiMgCu system. The coating was applied using a newly designed approach that combined high velocity oxy-fuel (HVOF) and plasma spraying processes. This hybrid technique enables the deposition of coatings with enhanced performance characteristics. The optical microscopy (OM) and scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM + EDS) revealed a strong adhesion and compaction between the multicomponent coating and the A6061 substrate. The new coating improved hardness by 50% and increased electrical conductivity by approximately 3.3 times compared to the as-cast alloy. Corrosion tests showed a lower corrosion rate, comparable to thermally treated A6061 alloy. Tribological tests indicated over 20% reduction in friction and over 50% reduction in wear rate. This suggests that multicomponent aluminum coatings could improve automotive and parts in contact with hydrogen by enhancing hydrogen fragilization resistance, corrosion resistance, electrical conductivity, and wear properties, with further optimization of thermal spraying potentially boosting performance even further. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Electrochemical Noise Analysis: An Approach to the Effectivity of Each Method in Different Materials.
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Jáquez-Muñoz, Jesús Manuel, Gaona-Tiburcio, Citlalli, Méndez-Ramírez, Ce Tochtli, Martínez-Ramos, Cynthia, Baltazar-Zamora, Miguel Angel, Santiago-Hurtado, Griselda, Estupinan-Lopez, Francisco, Landa-Ruiz, Laura, Nieves-Mendoza, Demetrio, and Almeraya-Calderon, Facundo
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DETERIORATION of materials ,TIME-domain analysis ,ALLOYS ,MICROSCOPY ,ELECTROCHEMICAL analysis - Abstract
Corrosion deterioration of materials is a major problem affecting economic, safety, and logistical issues, especially in the aeronautical sector. Detecting the correct corrosion type in metal alloys is very important to know how to mitigate the corrosion problem. Electrochemical noise (EN) is a corrosion technique used to characterize the behavior of different alloys and determine the type of corrosion in a system. The objective of this research is to characterize by EN technique different aeronautical alloys (Al, Ti, steels, and superalloys) using different analysis methods such as time domain (visual analysis, statistical), frequency domain (power spectral density (PSD)), and frequency–time domain (wavelet decomposition, Hilbert Huang analysis, and recurrence plots (RP)) related to the corrosion process. Optical microscopy (OM) is used to observe the surface of the tested samples. The alloys were exposed to 3.5 wt.% NaCl and H
2 SO4 solutions at room temperature. The results indicate that HHT and recurrence plots are the best options for determining the corrosion type compared with the other methods due to their ability to analyze dynamic and chaotic systems, such as corrosion. Corrosion processes such as passivation and localized corrosion can be differentiated when analyzed using HHT and RP methods when a passive system presents values of determinism between 0.5 and 0.8. Also, to differentiate the passive system from the localized system, it is necessary to see the recurrence plot due to the similarity of the determinism value. Noise impedance (Zn ) is one of the best options for determining the corrosion kinetics of one system, showing that Ti CP2 and Ti-6Al-4V presented 742,824 and 939,575 Ω·cm2 , while Rn presented 271,851 and 325,751 Ω·cm2 , being the highest when exposed to H2 SO4 . [ABSTRACT FROM AUTHOR]- Published
- 2024
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15. The Role of Digital Transformation and Digital Competencies in Organizational Sustainability: A Study of SMEs in Lima, Peru.
- Author
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Espina-Romero, Lorena, Ríos Parra, Doile, Gutiérrez Hurtado, Humberto, Peixoto Rodriguez, Egidio, Arias-Montoya, Francisco, Noroño-Sánchez, José Gregorio, Talavera-Aguirre, Rosa, Ramírez Corzo, Javier, and Vilchez Pirela, Rafael Alberto
- Abstract
Digitalization has significantly transformed businesses in the 21st century, but there are gaps in understanding how it affects human resource management and organizational culture in SMEs in Lima, Peru. This study aims to fill this gap by analyzing the influence of digital transformation, digital competencies, and digital HR management on the organizational culture of SMEs in Lima and how these factors contribute to organizational sustainability. Using a quantitative approach and structural equation modeling (SEM), 307 business leaders were surveyed between January and March 2024. The results show that digital transformation and digital competencies significantly influence digital HR management, which positively impacts organizational culture and promotes sustainable practices. Additionally, it was found that digital HR management mediates the relationship between digital transformation and organizational culture, as well as between digital competencies and organizational culture. It is crucial to invest in digital technologies and foster digital competencies to improve HR management and promote a sustainable organizational culture. In conclusion, digitalization redefines organizational culture and reinforces sustainability, especially in SMEs in Lima, Peru, providing new scientific value by demonstrating these mechanisms of change. [ABSTRACT FROM AUTHOR]
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- 2024
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16. Evaluation of Mis-Selection of End Vertebrae and Its Effect on Measuring Cobb Angle and Curve Length in Adolescent Idiopathic Scoliosis.
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Hurtado-Avilés, José, León-Muñoz, Vicente J., Santonja-Medina, Fernando, Raimondi, Paolo, and Martínez-Martínez, Francisco
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ADOLESCENT idiopathic scoliosis , *VERTEBRAE , *MEASUREMENT errors , *SPINE , *CURVATURE - Abstract
Background: The Cobb angle is critical in assessing adolescent idiopathic scoliosis (AIS) patients. This study aimed to evaluate the error in selecting the upper- and lower-end vertebrae on AIS digital X-rays by experienced and novice observers and its correlation with the error in measuring the Cobb angle and determining the length of the scoliotic curves. Methods: Using the TraumaMeter v.873 software, eight raters independently evaluated 68 scoliotic curves. Results: The error percentage in the upper-end vertebra selection was higher than for the lower-end vertebra (44.7%, CI95% 41.05–48.3 compared to 35%, CI95% 29.7–40.4). The mean bias error (MBE) was 0.45 (CI95% 0.38–0.52) for the upper-end vertebra and 0.35 (CI% 0.69–0.91) for the lower-end vertebra. The percentage of errors in the choice of the end vertebrae was lower for the experienced than for the novices. There was a positive correlation (r = 0.673, p = 0.000) between the error in selecting the end vertebrae and determining the length of the scoliotic curves. Conclusions: We can conclude that errors in selecting end vertebrae are common among experienced and novice observers, with a greater error frequency for the upper-end vertebrae. Contrary to the consensus, the accuracy of determining the length of the scoliotic curve is limited by the Cobb method's reliance on the correct selection of the end vertebrae. [ABSTRACT FROM AUTHOR]
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- 2024
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17. Homophobic Bullying, Traditional Bullying, Cyberbullying, and Health-Related Quality of Life (HRQoL) in Adolescents According to Their Sexual Orientation.
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Hurtado-Mellado, Almudena and Rodríguez-Hidalgo, Antonio J.
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QUALITY of life , *SEXUAL orientation , *SOCIAL acceptance , *HOMOSEXUALITY , *BULLYING , *CYBERBULLYING - Abstract
Recent studies suggest that traditional bullying, cyberbullying, and homophobic bullying lead to a low health-related quality of life (HRQoL) in adolescents. The present study aims to analyze this, paying particular attention to homophobic bullying, based on the sexual orientation of 815 adolescents who were asked to fill in a self-report questionnaire. In heterosexuals, both homophobic victimization and victimization were inversely related to different dimensions of HRQoL (moods and emotions, and school environment, respectively). In adolescents who were uncertain about their sexual orientation, there were inverse relationships between homophobic victimization and mood and social acceptance, and there was also a positive relationship between homophobic aggression and physical well-being. In homosexuals and bisexuals, homophobic victimization was inversely related to social acceptance, economic resources, and autonomy, while homophobic aggression was not related to HRQoL. The results obtained offer new insights, thanks to the use of a specific and validated instrument to record homophobic bullying that covers both homophobic victimization and homophobic aggression. In addition, the need to intervene in a holistic manner, involving political and social agents, as well as all actors implicated in the school environment, is discussed with a view to protecting adolescent health and promoting inclusive education. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Scale to Evaluate Employee Experience: Evidence of Validity and Reliability in Regular Basic Education Teachers in the Peruvian Context.
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Acuña-Hurtado, Nilton, García-Salirrosas, Elizabeth Emperatriz, Villar-Guevara, Miluska, and Fernández-Mallma, Israel
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ORGANIZATIONAL behavior , *CONFIRMATORY factor analysis , *TALENT management , *PSYCHOMETRICS , *TEACHER educators - Abstract
Research on employee experience is a topic that has been growing in recent decades. This study analyzes the validity and reliability of an employee experience scale in Peruvian teachers. The study had an instrumental design. The sample was comprised of 760 Peruvian teachers between 20 and 71 years old (M = 40.91; SD = 10.05), where men (36.1%) and women (63.9%) participated, recruited through non-probabilistic sampling. A validity and reliability analysis of the employee experience scale confirmed the three original factors (sensory experience, intellectual experience, and emotional experience). The KMO test reaches a high level (0.950 > 0.70), and the Bartlett test reaches a highly significant level (Sig. = 0.000). The scale also showed good internal consistency (α = 0.948 to 0.980; CR = 0.950 to 0.981; AVE = 0.864 to 0.878). Similarly, for the confirmatory factor analysis, a measurement adjustment was performed, obtaining excellent and acceptable fit indices for Model 2 for three factors (CMIN/DF = 4.764; CFI = 0.984; SRMR = 0.024; RMSEA = 0.070). This study provides a useful tool to measure the employee experience in a friendly way, using simple language to be applied to the Peruvian context. This study is considered an important contribution to organizational behavior and human talent management in educational circles. [ABSTRACT FROM AUTHOR]
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- 2024
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19. SAMPLID: A New Supervised Approach for Meaningful Place Identification Using Call Detail Records as an Alternative to Classical Unsupervised Clustering Techniques.
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Mendoza-Hurtado, Manuel, Romero-del-Castillo, Juan A., and Ortiz-Boyer, Domingo
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MOBILE learning , *CELL phones , *KNOWLEDGE base , *MACHINE learning , *SOUND recordings - Abstract
Data supplied by mobile phones have become the basis for identifying meaningful places frequently visited by individuals. In this study, we introduce SAMPLID, a new Supervised Approach for Meaningful Place Identification, based on providing a knowledge base focused on the specific problem we aim to solve (e.g., home/work identification). This approach allows to tackle place identification from a supervised perspective, offering an alternative to unsupervised clustering techniques. These clustering techniques rely on data characteristics that may not always be directly related to classification objectives. Our results, using mobility data provided by call detail records (CDRs) from Milan, demonstrate superior performance compared to applying clustering techniques. For all types of CDRs, the best results are obtained with the 20 × 20 subgrid, indicating that the model performs better when supplied with information from neighboring cells with a close spatial relationship, establishing neighborhood relationships that allow the model to clearly learn to identify transitions between cells of different types. Considering that it is common for a place or cell to be labeled in multiple categories at once, this supervised approach opens the door to addressing the identification of meaningful places from a multi-label perspective, which is difficult to achieve using classical unsupervised methods. [ABSTRACT FROM AUTHOR]
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- 2024
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20. Transcranial Photobiomodulation for Executive Function in Bipolar Disorder (TPEB): Study Protocol.
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Coelho, David Richer Araujo, Puerto, Aura Maria Hurtado, Vieira, Willians Fernando, Lohmann, Carlos Alberto, Shahab, Muhammad Hamza, Gersten, Maia Beth, Vahedifard, Farzan, McEachern, Kayla Marie, Clancy, Julie A., and Cassano, Paolo
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FUNCTIONAL magnetic resonance imaging ,REWARD (Psychology) ,EXECUTIVE function ,CEREBRAL circulation ,NEAR infrared radiation ,TRANSCRANIAL direct current stimulation - Abstract
Bipolar disorder (BD) is a debilitating psychiatric disorder characterized by mood disturbances and executive function deficits. Impairments in executive function, including impulsivity, significantly impact the daily lives of individuals with BD. Transcranial photobiomodulation (t-PBM) with near-infrared light offers a promising noninvasive neurostimulation approach to improve cognitive function. The Transcranial Photobiomodulation for Executive Function in Bipolar Disorder (TPEB) study aims to explore the potential of t-PBM in individuals with BD and executive function impairments. This study will include 20 adults with BD who will each receive one sham and one t-PBM session on the first day of stimulation (treatment day 1), followed by one daily t-PBM stimulation session for four days (treatment days 2 to 5). Cerebral blood flow changes will be evaluated using functional magnetic resonance imaging. Impulsivity, decision-making, and reward responsiveness will be assessed using the Barratt Impulsiveness Scale, the Iowa Gambling Task, and a gambling task that evaluates reward. The outcomes involve examining changes in cerebral blood flow, improvements in decision-making, and reductions in impulsivity and manic symptoms. The TPEB study aims to provide valuable insights into the potential of t-PBM as a therapeutic intervention to enhance executive function in BD. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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21. Age-Related Retinal Layer Thickness Changes Measured by OCT in APP NL-F/NL-F Mice: Implications for Alzheimer's Disease.
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Sánchez-Puebla, Lidia, de Hoz, Rosa, Salobrar-García, Elena, Arias-Vázquez, Alberto, González-Jiménez, María, Ramírez, Ana I., Fernández-Albarral, José A., Matamoros, José A., Elvira-Hurtado, Lorena, Saido, Takaomi C., Saito, Takashi, Nieto Vaquero, Carmen, Cuartero, María I., Moro, María A., Salazar, Juan J., López-Cuenca, Inés, and Ramírez, José M.
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ALZHEIMER'S disease ,OPTICAL coherence tomography ,LABORATORY mice ,DIABETIC retinopathy ,TRANSGENIC mice ,CASE-control method - Abstract
In Alzheimer's disease (AD), transgenic mouse models have established links between abnormalities in the retina and those in the brain. APP
NL-F/NL-F is a murine, humanized AD model that replicates several pathological features observed in patients with AD. Research has focused on obtaining quantitative parameters from optical coherence tomography (OCT) in AD. The aim of this study was to analyze, in a transversal case-control study using manual retinal segmentation via SD-OCT, the changes occurring in the retinal layers of the APPNL/F-NF/L AD model in comparison to C57BL/6J mice (WT) at 6, 9, 12, 15, 17, and 20 months of age. The analysis focused on retinal thickness in RNFL-GCL, IPL, INL, OPL, and ONL based on the Early Treatment Diabetic Retinopathy Study (ETDRS) sectors. Both APPNL-F/NL-F -model and WT animals exhibited thickness changes at the time points studied. While WT showed significant changes in INL, OPL, and ONL, the AD model showed changes in all retinal layers analyzed. The APPNL-F/NL-F displayed significant thickness variations in the analyzed layers except for the IPL compared to related WT. These thickness changes closely resembled those found in humans during preclinical stages, as well as during mild and moderate AD stages, making this AD model behave more similarly to the disease in humans. [ABSTRACT FROM AUTHOR]- Published
- 2024
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22. Molecular Adaptations of BDNF/NT-4 Neurotrophic and Muscarinic Pathways in Ageing Neuromuscular Synapses.
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Balanyà-Segura, Marta, Polishchuk, Aleksandra, Just-Borràs, Laia, Cilleros-Mañé, Víctor, Silvera, Carolina, Ardévol, Anna, Tomàs, Marta, Lanuza, Maria A., Hurtado, Erica, and Tomàs, Josep
- Subjects
BRAIN-derived neurotrophic factor ,PROTEIN kinase C ,MUSCARINIC receptors ,CHIMERIC proteins ,NEUROMUSCULAR system ,SARCOPENIA - Abstract
Age-related conditions, such as sarcopenia, cause physical disabilities for an increasing section of society. At the neuromuscular junction, the postsynaptic-derived neurotrophic factors brain-derived neurotrophic factor (BDNF) and neurotrophin 4 (NT-4) have neuroprotective functions and contribute to the correct regulation of the exocytotic machinery. Similarly, presynaptic muscarinic signalling plays a fundamental modulatory function in this synapse. However, whether or not these signalling pathways are compromised in ageing neuromuscular system has not yet been analysed. The present study analyses, through Western blotting, the differences in expression and activation of the main key proteins of the BDNF/NT-4 and muscarinic pathways related to neurotransmission in young versus ageing Extensor digitorum longus (EDL) rat muscles. The main results show an imbalance in several sections of these pathways: (i) a change in the stoichiometry of BDNF/NT-4, (ii) an imbalance of Tropomyosin-related kinase B receptor (TrkB)-FL/TrkB-T1 and neurotrophic receptor p 75 (p75
NTR ), (iii) no changes in the cytosol/membrane distribution of phosphorylated downstream protein kinase C (PKC)βI and PKCε, (iv) a reduction in the M2-subtype muscarinic receptor and P/Q-subtype voltage-gated calcium channel, (v) an imbalance of phosphorylated mammalian uncoordinated-18-1 (Munc18-1) (S313) and synaptosomal-associated protein 25 (SNAP-25) (S187), and (vi) normal levels of molecules related to the management of acetylcholine (Ach). Based on this descriptive analysis, we hypothesise that these pathways can be adjusted to ensure neurotransmission rather than undergoing negative alterations caused by ageing. However, further studies are needed to assess this hypothetical suggestion. Our results contribute to the understanding of some previously described neuromuscular functional age-related impairments. Strategies to promote these signalling pathways could improve the neuromuscular physiology and quality of life of older people. [ABSTRACT FROM AUTHOR]- Published
- 2024
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23. Optimal Area for a Rectangular Isolated Footing with an Eccentric Column and Partial Ground Compression.
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Moreno-Landeros, Victor Manuel, Luévanos-Rojas, Arnulfo, Santiago-Hurtado, Griselda, López-León, Luis Daimir, and Diaz-Gurrola, Eyran Roberto
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COLUMNS ,AXIAL loads ,STRUCTURAL engineering ,STRUCTURAL engineers ,PYRAMIDS - Abstract
Featured Application: This study is a practical tool for Structural Engineers to obtain the minimum contact area with the ground for rectangular isolated footings with an eccentric column. This manuscript aims to present a novel model to find the optimal area of a rectangular isolated footing with an eccentric column, taking into account that the footing is partially supported; that is, one part of the contact surface is compressed and the other part has zero pressure. The methodology is developed by integration and can also be verified using the geometric properties of a triangular-based pyramid to determine the axial load, the moments in the X and Y axes in terms of the available allowable soil pressure, the footing sides, the greatest distance on one of its sides in the X-direction where it crosses the neutral axis, the greatest distance on one of its sides in the Y-direction where it crosses the neutral axis, and the coordinates at the base of the footing. Four types of numerical problems are shown to find the optimal area of a rectangular footing with an eccentric column subjected to biaxial bending: (1) the column in the center of the footing; (2) the column on the edge of the footing in the X-direction; (3) the column on the edge of the footing in the Y-direction; and (4) the column in the corner of the footing. A comparison is presented of the new model against a model proposed by another author. The new model presents a reduction of up to 42.37% for the column in the center of the footing and up to 40.32% for the column in the corner of the footing compared to the model by the other authors. Therefore, the new model will be of great help to professionals in foundation design. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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24. Visual Performance of Children with Amblyopia after 6 Weeks of Home-Based Dichoptic Visual Training.
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Piñero, David P., Gil-Casas, Amparo, Hurtado-Ceña, Francisco J., and Molina-Martin, Ainhoa
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HOME care services ,REFRACTIVE errors ,DATA analysis ,RESEARCH funding ,CLINICAL trials ,STRABISMUS ,TREATMENT effectiveness ,DESCRIPTIVE statistics ,MANN Whitney U Test ,PRE-tests & post-tests ,RESEARCH methodology ,FRIEDMAN test (Statistics) ,STATISTICS ,VISUAL acuity ,AMBLYOPIA ,VISUAL perception ,DATA analysis software ,NONPARAMETRIC statistics ,CHILDREN - Abstract
Objectives: This study was aimed at analyzing the efficacy on the improvement of the visual function of a dichoptic online cloud-based platform for the treatment of amblyopia in anisometropic children. Methods: A quasi-experimental (pretest–post-test) study was conducted in 23 subjects with ages from 5 to 15 years old with anisometropic amblyopia combined with additional presence (2 subjects) or not (21 subjects) of microtropia. A total of 30 home-based training sessions of 30 min per session with Bynocs
® platform were prescribed for 6 weeks. Results: Amblyopic eye logMAR visual acuity (VA) significantly improved from 0.28 ± 0.24 to 0.13 ± 0.20 after the 6-week treatment (p < 0.001). At baseline, 60.9% of participants had VA in amblyopic eye of 0.20 logMAR or worse, whereas this percentage decreased to 21.7% after treatment. Binocular function (BF) significantly improved from 2.82 ± 1.11 to 2.32 ± 0.94 (p < 0.001). Mean compliance was 92%, 87% and 93% at 2, 4 and 6 weeks of treatment, respectively. Conclusions: In conclusion, home-based dichoptic training with the digital platform evaluated is an effective method to improve amblyopic VA and stereoacuity in children with anisometropic amblyopia combined or not with microtropia. [ABSTRACT FROM AUTHOR]- Published
- 2024
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25. Role of Quinoa (Chenopodium quinoa Willd) and Chickpea (Cicer arietinum L.) Ratio in Physicochemical Stability and Microbiological Quality of Fermented Plant-Based Beverages during Storage.
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Hurtado-Murillo, John, Franco, Wendy, and Contardo, Ingrid
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CHICKPEA ,PROTEIN stability ,LACTIC acid bacteria ,AMYLOPECTIN ,AMYLOSE ,QUINOA ,FERMENTED beverages - Abstract
Three different fermented plant-based beverages were prepared and stored for a long period (50 days) to assess the effect of the quinoa-to-chickpea ratio on physicochemical stability and microbiological quality. Physicochemical stability was evaluated based on pH, acidity, Brix degrees, water-holding capacity (WHC), viscosity, and viscoelasticity. At the end of the long-term storage period, the pH, acidity, and WHC remained stable. During the entire storage period, the beverages maintained good bacterial, fungal, and lactic acid bacteria (LAB) counts. Quinoa and chickpea flour ratios of 50% showed a higher viscosity (18 Pa.s) and WHC (65%) during short-term storage (0–30 d), indicating that the presence of chickpea flour had a positive effect on these parameters, possibly because chickpea starch contains higher amounts of amylose and long-branch chain amylopectin, which impacts the retrogradation pattern under acidic and refrigerated conditions. However, at the end of storage (50 days), the same blend had a higher acidity, lower viscosity (0.78 Pa.s), and lower LAB counts (~1 × 10
8 CFU/mL), indicating that the increase in chickpea flour had an adverse long-term effect on these parameters. These results suggest that although different ratios of plant sources can improve the physical aspects, they need to be incorporated in a balanced manner to avoid negative effects on both short- and long-term storage, owing to the incorporation of different types of starches and proteins affecting the stability of the system. [ABSTRACT FROM AUTHOR]- Published
- 2024
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26. Evaluating and Developing Transversal and Sustainability Competencies in University Classrooms to Empower New Generations.
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Tójar-Hurtado, Juan-Carlos, Lechuga-Jiménez, Clotilde, and Esteban-Ibáñez, Macarena
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SUSTAINABLE development ,HIGHER education ,COLLEGE teaching ,SUSTAINABILITY ,TRANSVERSAL lines - Abstract
This study examines the convergence of sustainability competencies in Spanish university curricula. The objective is to analyse and contrast opinions among university teaching staff and students from different fields of study in relation to the presence of competencies and curricular improvements concerning sustainability. The procedure comprised two surveys applied to professors (n
2 = 70) and students (n3 = 698). The instrument employed was the Register for Evaluating Curricular Sustainability at the University (RECSU), which permits the collation of data pertaining to sustainability-related transversal competencies, including sustainability values, the use of sustainable methodology in the classroom, the Sustainable Development Goals, and principles of action. In its entirety, this study's approach uses a mixed QUAN (qual) QUAL design. The results indicate which sustainability-aligned competencies and teaching practices are the most and least utilised by teaching staff, as well as which competencies present the greatest opportunity for improvement from the perspective of teaching staff. The findings highlight the relevance of empowering new generations through the development of transversal competencies in university studies. Sustainability competencies should be a priority to ensure that students are prepared to face future eco-social challenges. [ABSTRACT FROM AUTHOR]- Published
- 2024
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27. Exploring Shared Challenges of Empowered Patients and Entrepreneurs: Towards Diversity, Innovation, and Entrepreneurship in Post-Crisis Contexts.
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Hurtado Illanes, Marisol
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BUSINESSPEOPLE ,CULTURAL pluralism ,VIRTUAL communities ,SUSTAINABLE communities ,SOCIAL innovation - Abstract
This practical study explores the shared challenges faced by individuals managing chronic health conditions and entrepreneurs within the context of post-crisis periods, focusing on diversity, innovation, and individual empowerment. The aim is to develop a theoretical framework elucidating the mechanisms behind these challenges and their implications for fostering diversity, social innovation, and sustainability. Methodologically, an integrative approach was employed, drawing upon insights from psychology, sociology, health management, and entrepreneurship literature to conduct a comprehensive analysis. The research utilized mixed methods, including scoping reviews and qualitative analysis of virtual communities, to uncover the dynamic mechanisms that drive empowerment. This approach not only validated the theoretical framework but also proposed practical strategies to harness diversity, innovation, and entrepreneurship in post-crisis contexts. Drawing upon Dubin's methodology, the study structured the theoretical framework to highlight the interconnectedness of empowerment experiences within communities. The findings underscore the interconnectedness of empowerment experiences within communities and highlight the vital role of collaborative environments and support networks in promoting diversity, innovation, and entrepreneurship. Ultimately, this research contributes to the development of strategies that capitalize on cultural diversities and reap the benefits of different nationalities, knowledge areas, and cultures, particularly in the aftermath of crises. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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28. Management of skin lesions in patients with epidermolysis bullosa by topical treatment: systematic review and meta-analysis
- Author
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Universidad de Sevilla. Departamento de Enfermería, Universidad de Sevilla. Departamento de Podología, Universidad de Sevilla. CTS-1050: Cuidados Complejos, Cronicidad y Resultados en Salud, Universidad de Sevilla. CTS-601: Hermes, Pabón Carrasco, Manuel, Cáceres Matos, Rocío, Roche-Campos, Marta, Hurtado-Guapo, María Antonia, Ortiz Romero, Mercedes, Gordillo Fernández, Luis María, Pabón-Carrasco, Daniel, Castro Méndez, Aurora, Universidad de Sevilla. Departamento de Enfermería, Universidad de Sevilla. Departamento de Podología, Universidad de Sevilla. CTS-1050: Cuidados Complejos, Cronicidad y Resultados en Salud, Universidad de Sevilla. CTS-601: Hermes, Pabón Carrasco, Manuel, Cáceres Matos, Rocío, Roche-Campos, Marta, Hurtado-Guapo, María Antonia, Ortiz Romero, Mercedes, Gordillo Fernández, Luis María, Pabón-Carrasco, Daniel, and Castro Méndez, Aurora
- Abstract
Epidermolysis bullosa (EB) is the overarching term for a set of rare inherited skin fragility disorders that result from mutations in at least 20 different genes. Currently, there is no cure for any of the EB subtypes associated with various mutations. Existing therapies primarily focus on alleviating pain and promoting early wound healing to prevent potential complications. Consequently, there is an urgent need for innovative therapeutic approaches. The objective of this research was to assess the efficacy of various topical treatments in patients with EB with the goal of achieving wound healing. A secondary objective was to analyse the efficacy of topical treatments for symptom reduction. A literature search was conducted using scientific databases, including The Cochrane Library, Medline (Pubmed), Web of Science, CINHAL, Embase, and Scopus. The protocol review was registered in PROSPERO (ID: 418790), and inclusion and exclusion criteria were applied, resulting in the selection of 23 articles. Enhanced healing times were observed compared with the control group. No conclusive data have been observed on pain management, infection, pruritus episodes, and cure rates over time. Additionally, evidence indicates significant progress in gene therapies (B-VEC), as well as cell and protein therapies. The dressing group, Oleogel S-10, allantoin and diacerein 1%, were the most represented, followed by fibroblast utilisation. In addition, emerging treatments that improve the patient’s innate immunity, such as calcipotriol, are gaining attention. However, more trials are needed to reduce the prevalence of blistering and improve the quality of life of individuals with epidermolysis bullosa.
- Published
- 2024
29. Cricket Protein as an Innovative Emulsifier for Avocado Oil: Formulation and Characterization of Sustainable Emulsions
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Universidad de Sevilla. Departamento de Ingeniería Química, Trujillo-Cayado, Luis Alfonso, García Domínguez, Irene, Rodríguez Luna, Azahara María, Hurtado-Fernández, Elena, Santos García, Jenifer, Universidad de Sevilla. Departamento de Ingeniería Química, Trujillo-Cayado, Luis Alfonso, García Domínguez, Irene, Rodríguez Luna, Azahara María, Hurtado-Fernández, Elena, and Santos García, Jenifer
- Abstract
The use of cricket protein in emulsions is in line with the growing interest in sustainable food sources, as crickets require minimal resources and produce lower greenhouse gas emissions than traditional livestock. Research in this area suggests that incorporating cricket protein into emulsions not only improves their nutritional value but also contributes to the development of environmentally friendly and functional food products. This study proposes the use of cricket protein for the stabilization of emulsions formulated with avocado oil as a dispersed phase. This oil is rich in monounsaturated fats, particularly oleic acid, and a variety of bioactive compounds. In the first part of this study, we assessed the influence of the emulsifier concentration and found that 2 wt.% is the optimum because a depletion flocculation effect was produced. Subsequently, processing was optimized using ultrasonication so that the higher energy input produced emulsions with a droplet diameter of less than 700 nm. Finally, rhamsan gum was added to the formulation, producing emulgels with improved pseudoplastic behavior and physical stability. This study demonstrates that cricket protein in combination with rhamsan gum is capable of forming stable, low-droplet-size emulgels with potential applications in encapsulation systems.
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- 2024
30. A Tailored Approach to Leishmaniases Vaccination: Comparative Evaluation of the Efficacy and Cross-Protection Capacity of DNA vs. Peptide-Based Vaccines in a Murine Model
- Author
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Mas Zubiri, Alicia, Hurtado Morillas, Clara, Martínez Rodrigo, Abel, Orden Gutiérrez, José Antonio, Fuente López, Ricardo De La, Domínguez Bernal, Gustavo Ramón, Carrión Herrero, Francisco Javier, Mas Zubiri, Alicia, Hurtado Morillas, Clara, Martínez Rodrigo, Abel, Orden Gutiérrez, José Antonio, Fuente López, Ricardo De La, Domínguez Bernal, Gustavo Ramón, and Carrión Herrero, Francisco Javier
- Abstract
Zoonotic leishmaniases are a worldwide public health problem for which the development of effective vaccines remains a challenge. A vaccine against leishmaniases must be safe and affordable and should induce cross-protection against the different disease-causing species. In this context, the DNA vaccine pHisAK70 has been demonstrated to induce, in a murine model, a resistant phenotype against L. major, L. infantum, and L. amazonensis. Moreover, a chimeric multiepitope peptide, HisDTC, has been obtained by in silico analysis from the histone proteins encoded in the DNA vaccine and has showed its ability to activate a potent CD4+ and CD8+ T-cell protective immune response in mice against L. infantum infection. In the present study, we evaluated the plasmid DNA vaccine pHisAK70 in comparison with the peptide HisDTC (with and without saponin) against L. major and L. infantum infection. Our preliminary results showed that both formulations were able to induce a potent cellular response leading to a decrease in parasite load against L. infantum. In addition, the DNA candidate was able to induce better lesion control in mice against L. major. These preliminary results indicate that both strategies are potentially effective candidates for leishmaniases control. Furthermore, it is important to carry out such comparative studies to elucidate which vaccine candidates are the most appropriate for further development., COMUNIDAD DE MADRID, Depto. de Sanidad Animal, Fac. de Veterinaria, TRUE, pub
- Published
- 2024
31. A Tailored Approach to Leishmaniases Vaccination: Comparative Evaluation of the Efficacy and Cross-Protection Capacity of DNA vs. Peptide-Based Vaccines in a Murine Model
- Author
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Carrión Herrero, Francisco Javier, Mas Zubiri, Alicia, Hurtado Morillas, Clara, Martínez Rodrigo, Abel, Orden Gutiérrez, José Antonio, Fuente López, Ricardo De La, Domínguez Bernal, Gustavo Ramón, Carrión Herrero, Francisco Javier, Mas Zubiri, Alicia, Hurtado Morillas, Clara, Martínez Rodrigo, Abel, Orden Gutiérrez, José Antonio, Fuente López, Ricardo De La, and Domínguez Bernal, Gustavo Ramón
- Abstract
Author Contributions: All the authors listed contributed to this study. Conceptualization, A.M.-R. and A.M.; methodology, A.M.-R. and C.H.-M.; formal analysis, G.D.-B., A.M.-R., J.A.O. and C.H.-M.; writing—original draft preparation, A.M.-R. and A.M.; writing—review and editing, J.C., J.A.O., R.d.l.F. and G.D.-B.; project administration, J.C., R.d.l.F. and G.D.-B.; funding acquisition, J.C. and G.D.-B. All authors have read and agreed to the published version of the manuscript., Zoonotic leishmaniases are a worldwide public health problem for which the development of effective vaccines remains a challenge. A vaccine against leishmaniases must be safe and affordable and should induce cross-protection against the different disease-causing species. In this context, the DNA vaccine pHisAK70 has been demonstrated to induce, in a murine model, a resistant phenotype against L. major, L. infantum, and L. amazonensis. Moreover, a chimeric multiepitope peptide, HisDTC, has been obtained by in silico analysis from the histone proteins encoded in the DNA vaccine and has showed its ability to activate a potent CD4+ and CD8+ T-cell protective immune response in mice against L. infantum infection. In the present study, we evaluated the plasmid DNA vaccine pHisAK70 in comparison with the peptide HisDTC (with and without saponin) against L. major and L. infantum infection. Our preliminary results showed that both formulations were able to induce a potent cellular response leading to a decrease in parasite load against L. infantum. In addition, the DNA candidate was able to induce better lesion control in mice against L. major. These preliminary results indicate that both strategies are potentially effective candidates for leishmaniases control. Furthermore, it is important to carry out such comparative studies to elucidate which vaccine candidates are the most appropriate for further development., Comunidad de Madrid, PLATESA2-CM, S2018/BAA-4370, PID2019-106487RB-I00/AEI/10.13039/501100011033, Depto. de Sanidad Animal, Fac. de Veterinaria, TRUE, pub
- Published
- 2024
32. Nutritional and phytochemical composition of mediterranean wild vegetables after culinary treatment
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García Herrera, Patricia, Morales Gómez, Patricia, Cámara Hurtado, María De La Montaña, Fernánez-Ruiz, Virginia, Tardío, Javier, Sánchez Mata, María De Cortes, García Herrera, Patricia, Morales Gómez, Patricia, Cámara Hurtado, María De La Montaña, Fernánez-Ruiz, Virginia, Tardío, Javier, and Sánchez Mata, María De Cortes
- Abstract
Studies are scarce on the nutritional and phytochemical composition of wild edible Mediterranean plants after culinary processing. This work provides the nutritional composition after culinary treatment (including dietary fiber and mineral composition) and bioactive compounds (folates, vitamin C and organic acids) of wild Rumex pulcher L., Silene vulgaris (Moench) Garcke. leaves, and wild Asparagus acutifolius L., Bryonia dioica Jacq., Humulus lupulus L., Tamus communis L. young shoots. Shoots better preserved their nutrients than leaves, due to their different tissue structure. Fresh and cooked wild greens present high dietary fiber values, and remained at remarkable levels after boiling. Na, K, Mg and Zn were lost in about 50% due to culinary processing, while Ca, Cu, Fe and Mn were more stable. Boiled leaves of S. vulgaris remained as a good Mn source. A portion of 100 g of most of the cooked analyzed species could cover a relevant percentage of the daily requirement of folates (R. pulcher and A. acutifolius providing more than 80%) and vitamin C (T. communis and A. acutifolius providing more than 35%)., ALIMNOVA UCM Research Group, Depto. de Nutrición y Ciencia de los Alimentos, Fac. de Farmacia, TRUE, pub
- Published
- 2024
33. Piperacillin-Tazobactam as an Adjuvant in the Mechanical Treatment of Patients with Periodontitis: A Randomized Clinical Study
- Author
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Hurtado-Celotti, Dolores, Martínez Rodríguez, Natalia, Ruiz-Sáenz, Pedro Luis, Barona Dorado, Cristina, Juan Santos-Marino, José María Martínez-González, Martínez González, José María, Hurtado-Celotti, Dolores, Martínez Rodríguez, Natalia, Ruiz-Sáenz, Pedro Luis, Barona Dorado, Cristina, Juan Santos-Marino, José María Martínez-González, and Martínez González, José María
- Abstract
In this study, the aim was to evaluate the effects of the adjuvant piperacillin–tazobactam solution in the mechanical treatment of periodontitis. A single-blind split-mouth randomized study, it included 24 participants. All of them presented periodontitis stage III according to the 2018 World Workshop classification and the presence of at least one of the following periodontal pathogens: Aggregatibacter actinomycetemcomitans; Porphyromona gingivalis; Treponema denticola; Tannerella forsythia; Prevotella intermedia. The study established two groups: a control group (SRP: scaling and root planing) and a test group (SRP plus local piperacillin–tazobactam). The final recruitment included 11 women (45.8%) and 13 men (54.2%). The age range was between 25 and 72 years, and the mean age was 57 ± 10.20 years. Clinical controls were performed at 2 weeks, 3 months, and 6 months, repeating the SRP and applying the piperacillin–tazobactam solution again at the 3-month appointment. The clinical attachment level decreased by a mean of 2.13 ± 0.17 mm from the baseline to 6 months in the test group versus 1.63 ± 0.18 mm in the control group. The mean probing pocket depth decreased from 1.32 ± 0.09 mm in the test group, versus from 0.96 ± 0.14 mm on the control side. The plaque index in the test group decreased by 0.46 ± 0.04, while it decreased by an average of 0.31 ± 0.04 in the control group. In conclusion, the local use of piperacillin–tazobactam as complementary therapy produces better clinical results in patients with periodontitis. However, these results are not maintained over time, and so a more persistent local application is necessary., Depto. de Especialidades Clínicas Odontológicas, Fac. de Odontología, TRUE, pub
- Published
- 2024
34. Latent Microsporidia Infection Prevalence as a Risk Factor in Colon Cancer Patients
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Redondo, Fernando, Hurtado-Marcos, Carolina, Izquierdo, Fernando, Cuéllar Del Hoyo, María Del Carmen, Fenoy, Soledad, Sáez, Yanira, Magnet, Ángela, Galindo-Regal, Lorena, Uribe, Natalia, López-Bañeres, Manuel, Jiménez, Ana Isabel, Llombart-Cussac, Antonio, Águila, Carmen Del, Andreu-Ballester, Juan Carlos, Redondo, Fernando, Hurtado-Marcos, Carolina, Izquierdo, Fernando, Cuéllar Del Hoyo, María Del Carmen, Fenoy, Soledad, Sáez, Yanira, Magnet, Ángela, Galindo-Regal, Lorena, Uribe, Natalia, López-Bañeres, Manuel, Jiménez, Ana Isabel, Llombart-Cussac, Antonio, Águila, Carmen Del, and Andreu-Ballester, Juan Carlos
- Abstract
Microsporidia are opportunistic intracellular parasites, generating serious pathology in individuals with a compromised immune system. Infection by microsporidia inhibits p53 and Caspase 3, proteins involved in apoptosis and the cell cycle, which are vital in the malignant process of epithelial cells. The presence of microsporidia in the intestinal tissues of 87 colon cancer (CC) patients and 25 healthy controls was analyzed by real-time PCR and an immunofluorescence antibody test. Anti-Encephalitozoon antibodies were analyzed in serum samples by ELISA (enzyme linked immunosorbent assay). In 36 (41.3%) CC cases, microsporidia infections were identified in their tissues vs. no cases among control subjects (p < 0.0001). An increase in IgG and IgE anti-Encephalitozoon antibodies was found in patients with CC, which would demonstrate continuous and previous contact with the parasite. The high prevalence of microsporidia in tissues and the seroprevalence in patients with CC suggest a relationship between microsporidia and the etiopathogenesis of CC., Banco Santander Foundation-CEU Foundation, Depto. de Microbiología y Parasitología, Fac. de Farmacia, TRUE, pub
- Published
- 2024
35. Synergic Effect of the Antimicrobial Peptide ToAP2 and Fluconazole on Candida albicans Biofilms.
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do Nascimento Dias, Jhones, Hurtado Erazo, Fabián Andrés, Bessa, Lucinda J., Eaton, Peter, Leite, José Roberto de Souza de Almeida, Paes, Hugo Costa, Nicola, André Moraes, Silva-Pereira, Ildinete, and Albuquerque, Patrícia
- Subjects
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ANTIMICROBIAL peptides , *CANDIDA albicans , *SCANNING force microscopy , *BIOFILMS , *ATOMIC force microscopy , *ANTIFUNGAL agents - Abstract
Candida albicans is one of the agents of invasive candidiasis, a life-threatening disease strongly associated with hospitalization, particularly among patients in intensive care units with central venous catheters. This study aimed to evaluate the synergistic activity of the antifungal peptide ToAP2 combined with fluconazole against C. albicans biofilms grown on various materials. We tested combinations of different concentrations of the peptide ToAP2 with fluconazole on C. albicans biofilms. These biofilms were generated on 96-well plates, intravenous catheters, and infusion tubes in RPMI medium at two maturation stages. Scanning electron microscopy and atomic force microscopy were employed to assess the biofilm structure. We also evaluated the expression of genes previously proven to be involved in C. albicans biofilm formation in planktonic and biofilm cells after treatment with the peptide ToAP2 using qPCR. ToAP2 demonstrated a synergistic effect with fluconazole at concentrations up to 25 µM during both the early and mature stages of biofilm formation in 96-well plates and on medical devices. Combinations of 50, 25, and 12.5 µM of ToAP2 with 52 µM of fluconazole significantly reduced the biofilm viability compared to individual treatments and untreated controls. These results were supported by substantial structural changes in the biofilms observed through both scanning and atomic force microscopy. The gene expression analysis of C. albicans cells treated with 25 µM of ToAP2 revealed a decrease in the expression of genes associated with membrane synthesis, along with an increase in the expression of genes involved in efflux pumps, adhesins, and filamentation. Our results highlight the efficacy of the combined ToAP2 and fluconazole treatment against C. albicans biofilms. This combination not only shows therapeutic potential but also suggests its utility in developing preventive biofilm tools for intravenous catheters. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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36. Comparative Analysis of Viral Load and Cytokines during SARS-CoV-2 Infection between Pregnant and Non-Pregnant Women.
- Author
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Liu, Dakai, Li, Hui, Li, Xiaofeng, Rodriguez, George D., Pietz, Harlan, Fiel, Roberto Hurtado, Konadu, Eric, Singh, Vishnu, Loo, Florence, and Rodgers, William Harry
- Subjects
PREGNANT women ,VIRAL load ,REVERSE transcriptase polymerase chain reaction ,SARS-CoV-2 ,COVID-19 pandemic - Abstract
To better understand the vulnerabilities of pregnant women during the COVID-19 pandemic, we conducted a comprehensive, retrospective cohort study to assess differences in immune responses to SARS-CoV-2 infection between pregnant and non-pregnant women. Nasopharyngeal swabs and serum specimens from 90 pregnant and 278 age-matched non-pregnant women were collected from 15 March 2020 to 23 July 2021 at NewYork-Presbyterian Queens Hospital in New York City. Multiplex reverse transcription polymerase chain reaction, neutralizing antibody, and cytokine array assays were used to assess the incidence, viral load, antibody titers and profiles, and examine cytokine expression patterns. Our results show a lower incidence of SARS-CoV-2 infection in pregnant women compared with non-pregnant women. Pregnant women infected with SARS-CoV-2 exhibited a substantially lower viral load. In addition, the levels of both anti-spike protein receptor-binding domain IgG neutralizing antibodies and anti-N Protein IgG were elevated in pregnant women. Finally, cytokine profiling revealed differential expression of leptin across cohorts. These findings suggest that pregnancy is associated with distinct immune and virological responses to SARS-CoV-2 infection, characterized by lower infection rates, substantially lower viral loads, and enhanced antibody production. Differential cytokine expression indicates unique immune modulation in pregnant women. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
37. Methodological and Statistical Considerations for Cross-Sectional, Case–Control, and Cohort Studies.
- Author
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Pérez-Guerrero, Edsaúl Emilio, Guillén-Medina, Miryam Rosario, Márquez-Sandoval, Fabiola, Vera-Cruz, José María, Gallegos-Arreola, Martha Patricia, Rico-Méndez, Manuel Alejandro, Aguilar-Velázquez, José Alonso, and Gutiérrez-Hurtado, Itzae Adonai
- Subjects
DISEASE risk factors ,STATISTICS methodology ,CROSS-sectional method ,SCIENTIFIC observation ,INFECTIOUS disease transmission - Abstract
Epidemiological studies are essential in medicine and public health as they help identify risk factors and causes of diseases. Additionally, they are key to planning, implementing, and evaluating health interventions aimed at preventing and controlling the spread of diseases. Among these studies, analytical observational studies, such as cross-sectional, case–control, and cohort studies, are the most used. The validity of their results largely depends on the robustness of the design, execution, and statistical analysis. Objective: The objective of this study is to examine the most common errors in the selection of methodological design and statistical tests in analytical observational studies and to provide recommendations to correct them. Methodology: A comprehensive review of the available literature on methodology in epidemiological observational studies was conducted, focusing on cross-sectional, case–control, and cohort studies. Common errors in the selection of designs and statistical tests were identified and analyzed. Results and Conclusions: Errors in the selection of methodological design and statistical tests are common in epidemiological observational studies. Based on the identified errors, a series of recommendations is provided to improve the selection of methodological design and statistical tests, thereby increasing the reliability of the results in cross-sectional, case–control, and cohort studies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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38. TransPhoM-DS Study Grant Report: Rationale and Protocol for Investigating the Efficacy of Low-Power Transcranial Photobiomodulation on Language, Executive Function, Attention, and Memory in Down Syndrome.
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Vieira, Willians Fernando, Coelho, David Richer Araujo, Gersten, Maia, Puerto, Aura Maria Hurtado, Kalli, Stefani, Gonzalez-Garibay, Guillermo, McEachern, Kayla, Clancy, Julie A., Skotko, Brian G., Abbeduto, Leonard, Thurman, Angela John, Pulsifer, Margaret B., Corcoran, Elizabeth, Saltmarche, Anita E., Naeser, Margaret A., and Cassano, Paolo
- Subjects
PHOTOBIOMODULATION therapy ,EXECUTIVE function ,DOWN syndrome ,SHORT-term memory ,INTELLECTUAL disabilities ,TRANSCRANIAL direct current stimulation ,PREMATURE labor - Abstract
Down syndrome (DS) is the leading genetic cause of intellectual disability globally, affecting about 1 in every 800 births. Individuals with DS often face various neuropsychiatric conditions alongside intellectual disabilities due to altered brain development. Despite the diverse phenotypic expressions of DS, typical physical characteristics frequently influence language development and acquisition. EEG studies have identified abnormal oscillatory patterns in individuals with DS. Emerging interventions targeting the enhancement of gamma (40 Hz) neuronal oscillations show potential for improving brain electrical activity and cognitive functions in this population. However, effective cognitive interventions for DS remain scarce. Extensive research indicates that transcranial photobiomodulation (t-PBM) with near-infrared (NIR) light can penetrate deeply into the cerebral cortex, modulate cortical excitability, and enhance cerebral perfusion and oxygenation. Furthermore, t-PBM has been shown to improve cognitive functions such as language, attention, inhibition, learning, and memory, including working memory. This study presents the rationale and design of an ongoing randomized, sham-controlled clinical trial aimed at assessing the effectiveness of t-PBM using NIR light in enhancing the language abilities of individuals with DS. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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39. Exploring the Association between Parental Employment Status, Education Level, and Sensory Reactivity in Spanish Children Aged 3–7 Years: Findings from the InProS Study.
- Author
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Muñoz-Sánchez, Rocío, Hurtado-Pomares, Miriam, Juárez-Leal, Iris, Piñero, Jessica, Navarrete-Muñoz, Eva-María, and Valera-Gran, Desirée
- Subjects
RISK assessment ,PARENTS ,STATISTICAL correlation ,CHILDREN'S health ,POISSON distribution ,QUESTIONNAIRES ,TASTE ,FISHER exact test ,MULTIPLE regression analysis ,SENSORY disorders ,PARENT attitudes ,DISEASE prevalence ,CHI-squared test ,MANN Whitney U Test ,DESCRIPTIVE statistics ,SMELL ,RESEARCH ,CONFIDENCE intervals ,DATA analysis software ,EMPLOYMENT ,EDUCATIONAL attainment ,WELL-being ,DISEASE risk factors ,CHILDREN - Abstract
This study explored the association between parental employment status and education level and the prevalence of sensory reactivity (SR) in population-based sample of school-aged children. SR was assessed in 495 children using the parent-reported Short Sensory Profile (SSP) questionnaire. Children with SR were identified based on probable or definitive differences in total SSP and subscales. Association between parental employment and education level were explored using multiple Poisson regression models with robust variance, adjusted for potential confounders. The main findings showed that a mother's unemployment status was associated with higher prevalence of SR for the taste/smell sensitivity subscale (PR = 1.66, 95%CI: 1.08–2.56), and the low energy/weak (PR = 2.18, 95%CI: 1.31–3.49) subscale. A lower education level of a father was also associated with a higher prevalence of sensory problems on the tactile sensitivity subscale (PR
primary education = 2.68, 95%CI: 1.27–5.61; PRsecondary education = 1.96, 95%CI: 1.004–3.66) and the low energy/weak subscale (PRsecondary education = 1.95, 95%CI: 1.02–3.73). This study underscores the impact of parental employment and education on SR in school-aged children, offering insights for interventions and support systems aimed at improving their sensory functioning and overall well-being. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
40. Retinal Vascular and Structural Changes in the Murine Alzheimer's APP NL-F/NL-F Model from 6 to 20 Months.
- Author
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Sánchez-Puebla, Lidia, López-Cuenca, Inés, Salobrar-García, Elena, González-Jiménez, María, Arias-Vázquez, Alberto, Matamoros, José A., Ramírez, Ana I., Fernández-Albarral, José A., Elvira-Hurtado, Lorena, Saido, Takaomi C., Saito, Takashi, Nieto-Vaquero, Carmen, Cuartero, María I., Moro, María A., Salazar, Juan J., de Hoz, Rosa, and Ramírez, José M.
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ALZHEIMER'S disease ,DIABETIC retinopathy ,BRAIN diseases ,NATURAL history ,RETINA - Abstract
Alzheimer's disease (AD) may manifest retinal changes preceding brain pathology. A transversal case-control study utilized spectral-domain OCT angiography (SD-OCTA) and Angio-Tool software 0.6a to assess retinal vascular structures and OCT for inner and outer retina thickness in the APP
NL-F/NL-F AD model at 6, 9, 12, 15, 17, and 20 months old. Comparisons to age-matched wild type (WT) were performed. The analysis focused on the three vascular plexuses using AngiooTool and on retinal thickness, which was represented with the Early Treatment Diabetic Retinopathy Study (ETDRS) sectors. Compared to WT, the APPNL-F/NL-F group exhibited both vascular and structural changes as early as 6 months persisting and evolving at 15, 17, and 20 months. Significant vascular alterations, principally in the superficial vascular complex (SVC), were observed. There was a significant decrease in the vessel area and the total vessel length in SVC, intermediate, and deep capillary plexus. The inner retina in the APPNL-F/NL-F group predominantly decreased in thickness while the outer retina showed increased thickness in most analyzed time points compared to the control group. There are early vascular and structural retinal changes that precede the cognitive changes, which appear at later stages. Therefore, the natural history of the APPNL-F/NL-F model may be more similar to human AD than other transgenic models. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
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41. The Melting Behavior of Hydrogen Direct Reduced Iron in Molten Steel and Slag: An Integrated Computational and Experimental Study.
- Author
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Calderon Hurtado, Fabian Andres, Govro, Joseph, Emdadi, Arezoo, and O'Malley, Ronald J.
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SYNTHETIC natural gas ,LIQUID iron ,ELECTRIC arc ,ARC furnaces ,ELECTRIC furnaces ,SLAG - Abstract
Direct reduced iron (DRI) and hot briquetted iron (HBI) are essential feedstocks for tramp element control in the electric arc furnace (EAF). Due to greenhouse gas (GHG) concerns related to CO
2 emissions, hydrogen as a substitute for natural gas and a reductant in DRI production is being widely explored to reduce GHG emissions in ironmaking. This study examines the melting behavior of hydrogen DRI (H-DRI) pellets in the EAF containing low-carbon (0.1 wt.%) molten steel and molten slag. A computational heat transfer model was developed to predict the melting behavior of H-DRI pellets. To validate the model, a set of experimental laboratory simulations was conducted by immersing H-DRI in a molten steel bath and slag. The temperature history at the center of the pellet during melting and the shell thickness at different melting stages were utilized to validate the model. The simulation results agree with the experimental measurements of steel balls and H-DRI in different metallic molten steel and slag baths. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
42. In Silico Identification of Dysregulated miRNAs Targeting KRAS Gene in Pancreatic Cancer.
- Author
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Garibaldi-Ríos, Asbiel Felipe, Figuera, Luis E., Zúñiga-González, Guillermo Moisés, Gómez-Meda, Belinda Claudia, García-Verdín, Patricia Montserrat, Carrillo-Dávila, Irving Alejandro, Gutiérrez-Hurtado, Itzae Adonai, Torres-Mendoza, Blanca Miriam, and Gallegos-Arreola, Martha Patricia
- Subjects
GENETIC regulation ,GENE expression ,RAS oncogenes ,CANCER genes ,PANCREATIC cancer - Abstract
Pancreatic cancer (PC) is highly lethal, with KRAS mutations in up to 95% of cases. miRNAs inversely correlate with KRAS expression, indicating potential as biomarkers. This study identified miRNAs targeting KRAS and their impact on PC characteristics using in silico methods. dbDEMC identified dysregulated miRNAs in PC; TargetScan, miRDB, and PolymiRTS 3.0 identified miRNAs specific for the KRAS gene; and OncomiR evaluated the association of miRNAs with clinical characteristics and survival in PC. The correlation between miRNAs and KRAS was analysed using ENCORI/starBase. A total of 210 deregulated miRNAs were identified in PC (116 overexpressed and 94 underexpressed). In total, 16 of them were involved in the regulation of KRAS expression and 9 of these (hsa-miR-222-3p, hsa-miR-30a-5p, hsa-miR-30b-5p, hsa-miR-30e-5p, hsa-miR-377-3p, hsa-miR-495-3p, hsa-miR-654-3p, hsa-miR-877-5p and hsa-miR-885-5p) were associated with the clinical characteristics of the PC. Specifically, the overexpression of hsa-miR-30a-5p was associated with PC mortality, and hsa-miR-30b-5p, hsa-miR-377-3p, hsa-miR-495-3p, and hsa-miR-885-5p were associated with survival. Correlation analysis revealed that the expression of 10 miRNAs is correlated with KRAS expression. The dysregulated miRNAs identified in PC may regulate KRAS and some are associated with clinically relevant features, highlighting their potential as biomarkers and therapeutic targets in PC treatment. However, experimental validation is required for confirmation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
43. Corrosion of Anodized Titanium Alloys.
- Author
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Jáquez-Muñoz, Jesús Manuel, Gaona-Tiburcio, Citlalli, Mendez-Ramirez, Ce Tochtli, Carrera-Ramirez, Martha Guadalupe, Baltazar-Zamora, Miguel Angel, Santiago-Hurtado, Griselda, Lara-Banda, Maria, Estupiñan-Lopez, Francisco, Nieves-Mendoza, Demetrio, and Almeraya-Calderon, Facundo
- Subjects
ELECTROLYTIC corrosion ,TITANIUM corrosion ,TITANIUM alloys ,OXIDE coating ,CORROSION resistance - Abstract
Ti and Ti alloys are employed in demanding industries such as aerospace, automotive, biomedical, aeronautic, structural, naval, and chemical, thanks to their resistance to corrosion due to the formation of the TiO
2 film on the surface. Diverse research has established that different corrosive media could attack the oxide layer. One way to generate a stable, compact, and continuous oxide film is through anodizing treatment. The efficiency of anodization depends on diverse factors such as the microstructure, chemical composition of alloys, pH of electrolyte, time, and temperature of anodizing. This review aims to examine the corrosion resistance of the anodized layer on Ti and Ti alloys, with different parameters. The discussion is centered on the influence of the different parameters and alloy properties in the effectivity of anodizing when they are characterized by electrochemical techniques while studying the behavior of oxide. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
44. The Development and Content Validation of a Clinical Screening Scale to Identify Attention-Deficit Hyperactivity Disorder Cases Based on the Gender Perspective: An e-Delphi Study.
- Author
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Perez-Beltran, Meritxell, Roldán-Merino, Juan, Russi, Maria Eugenia, Rolandi, Maria Garau, Colome Roura, Roser, Sampaio, Francisco, Del Campo, Marta Domínguez, Farres-Tarafa, Mariona, Pardos, Barbara Hurtado, and Alda Díez, José Ángel
- Subjects
SELF-evaluation ,ATTENTION-deficit hyperactivity disorder ,RESEARCH methodology evaluation ,SEX distribution ,QUESTIONNAIRES ,CLASSIFICATION of mental disorders ,DESCRIPTIVE statistics ,EXPERIMENTAL design ,RESEARCH methodology ,MEDICAL screening ,EARLY diagnosis ,DELPHI method ,CHILDREN - Abstract
Background: Although many studies analyse gender differences in the clinical expression of Attention-Deficit Hyperactivity Disorder (ADHD) and prevalence studies show that girls with ADHD are underdiagnosed, there are no instruments that are sensitive to the detection of girls with ADHD. Objective: The objective of this study is to develop a self-report early detection instrument for boys and girls with ADHD aged 7 to 16, which includes the gender perspective and is sensitive to the detection of girls with ADHD. Methods: The scale was developed and the items that comprised it were created from the thematic analysis of ADHD and its evaluation in children based on the diagnostic criteria of the DSM-5-TR. A modified e-Delphi method involving a three-round web survey was used to establish a consensus on the content of the scale. Ten experts were recruited to form a professional panel. The panel members were asked to assess the differential symptomatology of ADHD in boys and girls, the dimensions to be evaluated, and the importance of scale items to evaluate the content. Results: A consensus was reached regarding 13 total items distributed in three dimensions: inattention; hyperactivity/impulsivity; and, a third dimension, internalisation, which includes symptoms most present in the expression of ADHD in girls. Conclusions: To the best of our knowledge, the development of this scale using the Delphi method is the first specific scale used for identifying ADHD that also addresses the gender perspective and the differential symptomatology between boys and girls. However, we must proceed to the analysis of psychometric properties, as the scale requires an exhaustive study of its reliability and validity. We can anticipate that this scale will provide relevant and reliable information that can be used for the identification of ADHD in both boys and girls. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
45. Phenanthrene-Induced Cytochrome P450 Genes and Phenanthrene Tolerance Associated with Arabidopsis thaliana CYP75B1 Gene.
- Author
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Cabello-Hurtado, Francisco and El Amrani, Abdelhak
- Subjects
PHENANTHRENE ,CYTOCHROME P-450 ,POLYCYCLIC aromatic hydrocarbons ,ARABIDOPSIS thaliana ,REACTIVE oxygen species ,BIOCHEMICAL substrates ,CYANIDIN - Abstract
Polycyclic aromatic hydrocarbons (PAHs) form an important group of organic pollutants due to their distribution in the environment and their carcinogenic and/or mutagenic effects. In order to identify at the molecular level some of the players in the biodegradation and tolerance response to PAHs in plants, we have phenotyped 32 Arabidopsis thaliana T-DNA mutant lines corresponding to 16 cytochrome P450 (CYP) genes that showed to be differentially expressed under contrasted stress conditions induced by phenanthrene, a 3-ring PAH. This screening has allowed us to identify CYP75B1 (At5g07990) T-DNA mutants as the only ones being sensitive to phenanthrene-induced stress, supporting that CYP75B1 protein is necessary for PAH tolerance. CYP75B1 codes for a 3′flavonol hydroxylase. CYP75B1 gene was heterologously expressed on yeast in order to investigate whether it affects the A. thaliana response to phenanthrene by participating in its metabolization. Heterologously-produced CYP75B1 enzyme shows to be catalytically efficient against its physiological substrates (e.g., naringenin) but unable to metabolize phenanthrene or 9-phenanthrenol. In contrast, CYP75B1 seems rather involved in phenanthrene tolerance as a crucial element by regulating concentration of antioxidants through the production of 3′-hydroxylated flavonoids such as quercetin and cyanidin. In particular, we report a highly increased generation of reactive oxygen species (H
2 O2 and singlet oxygen) in cyp75b1 mutants compared to control plants in response to phenanthrene treatment. Overall, CYP75B1 shows to play an important role in the response to the deleterious effects of phenanthrene exposure and this is related to oxidative stress sensitivity rather than metabolization. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
46. Biological Activity of Complexes Involving Nitro-Containing Ligands and Crystallographic-Theoretical Description of 3,5-DNB Complexes.
- Author
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Fonseca-López, Daniela, Lozano, Johan D., Macías, Mario A., Muñoz-Castro, Álvaro, MacLeod-Carey, Desmond, Nagles, Edgar, and Hurtado, John
- Subjects
NITROBENZOIC acid ,CRYSTAL structure ,NATURAL immunity ,DRUG resistance ,COMMUNICABLE diseases - Abstract
Drug resistance in infectious diseases developed by bacteria and fungi is an important issue since it is necessary to further develop novel compounds with biological activity that counteract this problem. In addition, new pharmaceutical compounds with lower secondary effects to treat cancer are needed. Coordination compounds appear to be accessible and promising alternatives aiming to overcome these problems. In this review, we summarize the recent literature on coordination compounds based on nitrobenzoic acid (NBA) as a ligand, its derivatives, and other nitro-containing ligands, which are widely employed owing to their versatility. Additionally, an analysis of crystallographic data is presented, unraveling the coordination preferences and the most effective crystallization methods to grow crystals of good quality. This underscores the significance of elucidating crystalline structures and utilizing computational calculations to deepen the comprehension of the electronic properties of coordination complexes. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
47. Critically Small Contemporaneous Effective Population Sizes Estimated for Stocks of the African Bonytongue in Western Africa.
- Author
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Hurtado, Luis A., Mateos, Mariana, Caballero, Isabel C., Oladimeji, Tofunmi E., Adite, Alphonse, Awodiran, Michael O., Winemiller, Kirk O., and Hamilton, Matthew B.
- Subjects
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FISHERIES , *LINKAGE disequilibrium , *FOOD security , *INBREEDING , *CONFIDENCE intervals - Abstract
Inland capture fisheries play a critical role in supporting food security and livelihoods in Africa. Therefore, it is important to evaluate the genetic health of exploited fish populations. The African bonytongue, Heterotis niloticus, supports important commercial and subsistence fisheries in western Africa. However, sharp declines in stocks have been reported. Herein, we estimate contemporary effective population sizes (Ne) of four Heterotis populations in Nigeria, three in Benin, and five in Cameroon using Linkage Disequilibrium methods. Ne estimates were used to assess genetic short-term (i.e., inbreeding depression) and long-term (i.e., loss of evolutionary potential) risks. Ne point estimates obtained with the best estimator (out of 16), as determined by computer simulations, were <50 (range = 5.1–36.2) for nine of the twelve populations examined, which is below the minimum recommended for avoiding the potential deleterious effects of inbreeding depression (original criterion Ne ≥ 50, revised to Ne ≥ 100); and well below the minimum recommended for populations to retain evolutionary potential (original criterion Ne ≥ 500; revised to Ne ≥ 1000). The lower bound of the confidence interval for two of the remaining populations was below the minimum recommended to retain evolutionary potential (with the point estimate of one of them also below this threshold), and for some methods, values were lower than the minimum recommended to avoid inbreeding depression. Accordingly, our results suggest that urgent conservation and management plans are needed to guarantee the persistence and sustainability of the H. niloticus populations examined. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
48. Silicon-Mediated Adjustments in C:N:P Ratios for Improved Beetroot Yield under Ammonium-Induced Stress.
- Author
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Olivera-Viciedo, Dilier, Salas Aguilar, Daimy, de Mello Prado, Renato, Peña Calzada, Kolima, Calero Hurtado, Alexander, de Cássia Piccolo, Marisa, Bomfim Soares, Mariana, Lizcano Toledo, Rodolfo, Alves, Guilherme Ribeiro, Ferreira, Daniele, Rodrigues, Rosane, and de Moura Zanine, Anderson
- Subjects
BEETS ,NITROGEN ,CULTIVATED plants ,BLOCK designs ,AMINO acids ,STOICHIOMETRY ,SUGAR beets - Abstract
Nitrogen (N) holds a prominent position in the metabolic system of plants, as it is a main constituent of amino acids, which are the basic building blocks of proteins and enzymes. Plants primarily absorb N in the form of ammonium (NH
4 + ) and nitrate (NO3 − ). However, most plants exhibit severe toxicity symptoms when exposed to NH4 + as the sole N source. Addressing NH4 + stress requires effective strategies, and the use of silicon (Si) has shown promising results. However, there is a lack of underlying studies on the impact of NH4 + toxicity on C:N:P stoichiometric balance and the role of Si in these ratios. In this study, we explored the effects of varying NH4 + concentrations (1, 7.5, 15, 22.5, and 30 mmol L−1 ) on the C:N:P stoichiometry and yield of beetroot in hydroponic conditions. Additionally, we investigated whether the application of Si (2 mmol L−1 ) could mitigate the detrimental effects caused by toxic NH4 + levels. The experiment followed a randomized block design based on a 5 × 2 factorial scheme with four replicates. Results revealed that in the presence of Si, both [N] and [P] significantly increased in shoots and roots, peaking at 15 mmol L−1 of NH4 + in the nutrient solution. While shoot [C] remained stable, root [C] increased with NH4 + concentrations of 22.5 and 30 mmol L−1 , respectively. Moreover, shoot and root [Si] increased with higher NH4 + levels in the nutrient solution. The findings underscored homeostatic instability under the highest NH4 + levels, particularly in plants cultivated without Si in the nutritive solution, leading to a reduction in both shoot and root dry matter production. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
49. Neurodegenerative Diseases: Unraveling the Heterogeneity of Astrocytes.
- Author
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Santiago-Balmaseda, Alberto, Aguirre-Orozco, Annai, Valenzuela-Arzeta, Irais E., Villegas-Rojas, Marcos M., Pérez-Segura, Isaac, Jiménez-Barrios, Natalie, Hurtado-Robles, Ernesto, Rodríguez-Hernández, Luis Daniel, Rivera-German, Erick R., Guerra-Crespo, Magdalena, Martinez-Fong, Daniel, Ledesma-Alonso, Carlos, Diaz-Cintra, Sofía, and Soto-Rojas, Luis O.
- Subjects
NEURODEGENERATION ,ASTROCYTES ,HOMEOSTASIS ,CENTRAL nervous system ,CENTRAL nervous system injuries ,HETEROGENEITY - Abstract
The astrocyte population, around 50% of human brain cells, plays a crucial role in maintaining the overall health and functionality of the central nervous system (CNS). Astrocytes are vital in orchestrating neuronal development by releasing synaptogenic molecules and eliminating excessive synapses. They also modulate neuronal excitability and contribute to CNS homeostasis, promoting neuronal survival by clearance of neurotransmitters, transporting metabolites, and secreting trophic factors. Astrocytes are highly heterogeneous and respond to CNS injuries and diseases through a process known as reactive astrogliosis, which can contribute to both inflammation and its resolution. Recent evidence has revealed remarkable alterations in astrocyte transcriptomes in response to several diseases, identifying at least two distinct phenotypes called A1 or neurotoxic and A2 or neuroprotective astrocytes. However, due to the vast heterogeneity of these cells, it is limited to classify them into only two phenotypes. This review explores the various physiological and pathophysiological roles, potential markers, and pathways that might be activated in different astrocytic phenotypes. Furthermore, we discuss the astrocyte heterogeneity in the main neurodegenerative diseases and identify potential therapeutic strategies. Understanding the underlying mechanisms in the differentiation and imbalance of the astrocytic population will allow the identification of specific biomarkers and timely therapeutic approaches in various neurodegenerative diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
50. Patient Safety Culture from a Nursing Perspective in a Chilean Hospital.
- Author
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Hurtado-Arenas, Paulina, Guevara, Miguel R., and González-Chordá, Víctor M.
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PUBLIC hospitals ,CORPORATE culture ,CROSS-sectional method ,PATIENT safety ,MEDICAL quality control ,MEDICAL specialties & specialists ,HOSPITAL nursing staff ,KRUSKAL-Wallis Test ,QUESTIONNAIRES ,SEX distribution ,NURSING ,QUANTITATIVE research ,DESCRIPTIVE statistics ,MANN Whitney U Test ,WORK experience (Employment) ,WORKING hours ,NURSES' attitudes ,RESEARCH methodology ,INFERENTIAL statistics ,ADVERSE health care events ,DATA analysis software ,NONPARAMETRIC statistics ,REGRESSION analysis ,EDUCATIONAL attainment ,HOSPITAL wards ,EMPLOYMENT - Abstract
Patient safety culture is relevant both in the delivery of care and in the training of nursing staff, its purpose being to prevent and reduce risks associated with health care. This research aims to evaluate patient safety culture from the perspective of the nursing teams in a highly complex public hospital in the city of Valparaíso, Chile. A cross-sectional study with a quantitative approach applying descriptive, bivariate, and inferential statistical analysis was conducted on 259 nurses and nursing assistants from 13 adult medical–surgical units of the Carlos Van Buren hospital. The participants were obtained through a non-probabilistic convenience sample, answering the hospital survey on Patient Safety Culture version 2.0 (HSOPS 2.0), adapted to the Chilean population. The best-evaluated dimension was communication and receptivity; the worst was the support administrators provide for patient safety. This study identified the weaknesses and strengths of the hospital, the most worrying weakness being the shortage of human capital, material, and financial resources necessary to improve patient safety. This study was not registered. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
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