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Response surface tests were used to determine the optimal conditions for Bacillus subtilis solid-state fermentation of highland barley bran. The polyphenol composition and antioxidant activity of the fermented bran were also assessed. The results showed that the optimal fermentation conditions for highland barley bran were 10% inoculum, a liquid–feed ratio of 1.80, a fermentation temperature of 30 °C, and a fermentation time of 93.5 h. Under these conditions, the polyphenol content of highland barley bran was 12.43 mg/g. After fermentation, the ferulic acid content of the bran decreased, catechol and gallic acid contents increased significantly, and catechins shifted from the bound state to the free state. In addition, solid-state fermentation improved the antioxidant capacity of highland barley bran, and the DPPH• radical scavenging rate, Fe ion-reducing capacity, and hydroxyl radical scavenging rate of highland barley bran increased after fermentation. [ABSTRACT FROM AUTHOR]

The functional components in tartary buckwheat leaf powder can give flour products higher nutritional value. To comprehensively realize the high-value utilization of tartary buckwheat and its by-products, electric stone mill powder (EMP), ultra-fine mill powder (UMP), steel mill powder (SMP), and grain mill powder (GMP) from tartary buckwheat leaves were used in the preparation of wheat dough, and this was used to explore their effects on dough properties and protein microstructure. With an increase in tartary buckwheat leaf powder, the hydration characteristics, protein weakening rate, and starch gelatinization characteristics of the dough changed, and the water holding capacity and swelling capacity decreased. The retrogradation value increased, which could prolong the shelf life of related products. The water solubility of the dough showed an upward trend and was the lowest at 10% UMP. The addition of UMP produced a more uniform dough stability time and the lowest degree of protein weakening, which made the dough more resistant to kneading. An increasing amount of tartary buckwheat leaf powder augmented the free sulfhydryl content of the dough and decreased the disulfide bond content. The disulfide bond content of the dough containing UMP was higher than that of the other doughs, and the stability of the dough was better. The peaks of the infrared spectrum of the dough changed after adding 10% UMP and 20% EMP. The content of α-helical structures was the highest at 10% UMP, and the content of ordered structures was enhanced. The polymerization of low molecular weight proteins to form macromolecular polymers led to a reduction in surface hydrophobic regions and the aggregation of hydrophobic groups. The SEM results also demonstrated that at 10% tartary buckwheat leaf powder, the addition of UMP was significantly different from that of the other three leaf powders, and at 20%, the addition of EMP substantially altered the structure of the dough proteins. Considering the effects of different milling methods and different added amounts of tartary buckwheat leaf powder on various characteristics of dough, 10% UMP is the most suitable amount to add to the dough. [ABSTRACT FROM AUTHOR]

Recently, artificial exosomes have been developed to overcome the challenges of natural exosomes, such as production scalability and stability. In the production of artificial exosomes, the incorporation of membrane proteins into lipid nanostructures is emerging as a notable approach for enhancing biocompatibility and treatment efficacy. This study focuses on incorporating HEK293T cell-derived membrane proteins into liposomes to create membrane-protein-bound liposomes (MPLCs), with the goal of improving their effectiveness as anticancer therapeutics. MPLCs were generated by combining two key elements: lipid components that are identical to those in conventional liposomes (CLs) and membrane protein components uniquely derived from HEK293T cells. An extensive comparison of CLs and MPLCs was conducted across multiple in vitro and in vivo cancer models, employing advanced techniques such as cryo-TEM (tramsmission electron microscopy) imaging and FT-IR (fourier transform infrared spectroscopy). MPLCs displayed superior membrane fusion capabilities in cancer cell lines, with significantly higher cellular uptake. Additionally, MPLCs maintained their morphology and size better than CLs when exposed to FBS (fetal bovine serum), suggesting enhanced serum stability. In a xenograft mouse model using HeLa and ASPC cancer cells, intravenous administration of MPLCs MPLCs accumulated more in tumor tissues, highlighting their potential for targeted cancer therapy. Overall, these results indicate that MPLCs have superior tumor-targeting properties, possibly attributable to their membrane protein composition, offering promising prospects for enhancing drug delivery efficiency in cancer treatments. This research could offer new clinical application opportunities, as it uses MPLCs with membrane proteins from HEK293T cells, which are known for their efficient production and compatibility with GMP (good manufacturing practice) standards. [ABSTRACT FROM AUTHOR]

The river and lake chief system offers a valuable policy toolkit to mitigate the degradation of water ecology, thereby bolstering water resource management for sustainable water development in China. To evaluate the effects of implementation and improve policy, this study took Beijing as a typical case and conducted a quantitative assessment using multidimensional data. The findings suggest that while the river and lake chief system in Beijing is effective and has significantly contributed to the ecological management of rivers and lakes, there are also notable regional disparities and urban–rural divergences. In addition, human activities are the main sources of environmental pollution in rivers and lakes, which should be the focus of the river and lake chief system. The river and lake chief system needs to embed more public participation and cooperative governance. This research aids in better understanding China's river and lake chief system for both researchers and practitioners, facilitating the advancement of the knowledge body of global water policy and governance. [ABSTRACT FROM AUTHOR]

Currently, many virtual simulation design studies of compression pants do not consider pressure distribution and human body characteristics. Therefore, this study aimed to optimize the simulation design accuracy of compression pants by investigating female body characteristics to improve the pressure distribution and enhance comfort. Firstly, we divided the body part features into flexible and rigid parts, performed compression relationship analysis between the material and the body, and collected qualitative and quantitative data related to the potential influencing factors. Subsequently, by conducting correlation analysis of the data, a pressure prediction model was established to address the pressure value errors in the simulation data. The research results showed that there was a significant difference between the real and virtual pressures in the flexible parts of the female body, and that the real pressure was closely related to the elasticity and thickness properties of the material. By optimizing virtual pressure values, the consistency between the virtual pressure and real test results can be significantly improved. The accurate prediction and optimization of pressure values can lead to the reduction of material waste and energy consumption during the manufacturing process. [ABSTRACT FROM AUTHOR]

We present a series of TCAD analysis of gallium nitride (GaN) heterojunction bipolar transistors (HBTs) that investigates the impact of various key parameters on the gain characteristics, output characteristics, and breakdown characteristics. It has been observed that the DC gain of the AlGaN/GaN HBTs exhibits a non-linear relationship with the increase in the Al fraction. Specifically, the DC gain initially rises, then declines after reaching its peak value at approximately 7%. By optimizing the concentration of the base and the concentration and thickness of the collector epitaxial layer, it is possible to achieve devices with breakdown voltages of 1270 V (with a collector thickness of 6 μm and a carrier concentration of 2 × 1016 cm−3), specific on-resistance of 0.88 mΩ·cm2, and a current gain of 73. In addition, an investigation on breakdown characteristics is conducted for HBTs with two types of substrates, namely QV-HBTs and FV-HBTs, at different inclinations of the ramp. We propose that critical angles are 79° and 69° to prevent the surface breakdown of the device, which helps to achieve an avalanche in GaN HBTs. We anticipate that the aforementioned findings will offer valuable insights for designing GaN-based power HBTs with elevated breakdown thresholds, heightened current densities, and increased power capabilities. [ABSTRACT FROM AUTHOR]

Papillary thyroid carcinoma (PTC) is the most common pathological type of thyroid cancer. PTC patients with extrathyroidal extension (ETE) are associated with poor prognoses. The preoperative accurate prediction of ETE is crucial for helping the surgeon decide on the surgical plan. This study aimed to establish a novel clinical-radiomics nomogram based on B-mode ultrasound (BMUS) and contrast-enhanced ultrasound (CEUS) for the prediction of ETE in PTC. A total of 216 patients with PTC between January 2018 and June 2020 were collected and divided into the training set (n = 152) and the validation set (n = 64). The least absolute shrinkage and selection operator (LASSO) algorithm was applied for radiomics feature selection. Univariate analysis was performed to find clinical risk factors for predicting ETE. The BMUS Radscore, CEUS Radscore, clinical model, and clinical-radiomics model were established using multivariate backward stepwise logistic regression (LR) based on BMUS radiomics features, CEUS radiomics features, clinical risk factors, and the combination of those features, respectively. The diagnostic efficacy of the models was assessed using receiver operating characteristic (ROC) curves and the DeLong test. The model with the best performance was then selected to develop a nomogram. The results show that the clinical-radiomics model, which is constructed by age, CEUS-reported ETE, BMUS Radscore, and CEUS Radscore, showed the best diagnostic efficiency in both the training set (AUC = 0.843) and validation set (AUC = 0.792). Moreover, a clinical-radiomics nomogram was established for easier clinical practices. The Hosmer–Lemeshow test and the calibration curves demonstrated satisfactory calibration. The decision curve analysis (DCA) showed that the clinical-radiomics nomogram had substantial clinical benefits. The clinical-radiomics nomogram constructed from the dual-modal ultrasound can be exploited as a promising tool for the pre-operative prediction of ETE in PTC. [ABSTRACT FROM AUTHOR]

Background: Hepatocellular carcinoma (HCC) is a malignant tumor associated with high morbidity and mortality. Therefore, it is of great importance to develop effective prognostic models and guide clinical treatment in HCC. Protein lactylation is found in HCC tumors and is associated with HCC progression. Methods: The expression levels of lactylation-related genes were identified from the TCGA database. A lactylation-related gene signature was constructed using LASSO regression. The prognostic value of the model was assessed and further validated in the ICGC cohort, with the patients split into two groups based on risk score. Glycolysis and immune pathways, treatment responsiveness, and the mutation of signature genes were analyzed. The correlation between PKM2 expression and the clinical characteristics was investigated. Results: Sixteen prognostic differentially expressed lactylation-related genes were identified. An 8-gene signature was constructed and validated. Patients with higher risk scores had poorer clinical outcomes. The two groups were different in immune cell abundance. The high-risk group patients were more sensitive to most chemical drugs and sorafenib, while the low-risk group patients were more sensitive to some targeted drugs such as lapatinib and FH535. Moreover, the low-risk group had a higher TIDE score and was more sensitive to immunotherapy. PKM2 expression correlated with clinical characteristics and immune cell abundance in the HCC samples. Conclusions: The lactylation-related model exhibited robust predictive efficiency in HCC. The glycolysis pathway was enriched in the HCC tumor samples. A low-risk score indicated better treatment response to most targeted drugs and immunotherapy. The lactylation-related gene signature could be used as a biomarker for the effective clinical treatment of HCC. [ABSTRACT FROM AUTHOR]

Although public–private partnerships (PPPs) for tourism have received extensive attention, there has been limited research on their application to tourist toilets. The purpose of this study was to systematically explore the applicability of PPPs with respect to the construction and operation of tourist toilets. The study conducted field investigations and semi-structured interviews in the Qingdao Laoshan Scenic Area (LSA), where purposive sampling techniques were used to manage participant selection and expand sample sizes. It finds that first, the use of PPPs to build tourist toilets is new and has shown both development potential and challenges. Second, the use of PPPs to provide tourist toilets is not yet mature owing to management and supply inefficiencies. Third, the DBTO model in the Laoshan case offers a significant methodological value for similar PPP projects, which could be replicated and promoted more widely when certain conditions are met. This study has filled a research gap in the global toilet revolution and shows a different path from the investment guarantee system in developed Western countries and the Guilin model in China. This application of a PPP in provision of tourist toilets also broadens the knowledge base of researchers studying PPPs in the field of tourism and could lead to the development of a policy approach with respect to PPPs' application in the toilet revolution and regional tourism development. [ABSTRACT FROM AUTHOR]

Infrastructure financialization is an important economic phenomenon in urbanization and urban financialization. The existing studies are mainly focused on qualitative and theoretical analysis around the world. To quantitatively evaluate the impact of infrastructure financialization on uneven regional development, this study firstly measures the characteristics of uneven regional development in China, then we use the Tobit model to analyze the impact of infrastructure financialization based on panel data from 2006 to 2019. The results find that infrastructure financialization plays a significant role in uneven regional development in China. Low infrastructure financialization constrains the economic growth and urbanization of underdeveloped regions. This study not only contributes to the knowledge body of global financialization theory, but also provides a scientific basis for the optimization of infrastructure development both in China and the Global South. [ABSTRACT FROM AUTHOR]

Inerter is a novel type of mechanical element. As the important composition of vibration control systems, inerters are widely used. Meanwhile, the operation security issue of the inerter is becoming increasingly prominent. Dynamic-breakdown of the inerter (DBoI) means that the inerter's two terminals come into contact, which can cause a huge impact force and destroy the system. The ball-screw inerter is an essential protype of inerters. The existence of dynamic-breakdown of a single inerter has been already proved by previous research. To confirm whether DBoI exists in the inerter-spring-damper (ISD) system, the research focuses on the dynamic-breakdown of a ball-screw inerter (DBoBSI) in the ISD system. The dynamic model of the DBoI in the ISD system is established and analyzed. On account of the model and analysis, DBoBSI is analyzed. A novel experimental system is established and experiments of DBoBSI in the ISD system are carried out. The coefficient of restitution et changes slightly with a value of about 0.5. The variation of momentum is nearly equal to the impulse, with an error within 15%. On account of the experimental data collected, the conclusions are that there is a DBoBSI in the ISD system, and the impact force during DBoI in the ISD system ought to be avoided. [ABSTRACT FROM AUTHOR]

Background: Ovarian cancer is the most lethal gynecological tumor, with a poor prognosis due to the lack of early symptoms, resistance to chemotherapy, and recurrence. Ferroptosis belongs to the regulated cell death family, and is characterized by iron-dependent processes. Here, comprehensive bioinformatics analysis was applied to explore a valuable prognostic model based on ferroptosis-related genes, which was further validated in clinical OC samples. Methods: mRNA data of normal and ovarian tumor samples were obtained separately from the GTEx and TCGA databases. The least absolute shrinkage and selection operator (LASSO) cox regression was applied to construct the prognostic model based on ferroptosis-associated genes. Expression of ALOX12 in OC cell lines, as well as cell functions, including proliferation and migration, were examined. Finally, the prognostic efficiency of the model was assessed in the clinical tissues of OC patients. Results: A gene signature consisting of ALOX12, RB1, DNAJB6, STEAP3, and SELENOS was constructed. The signature divided TCGA, ICGC, and GEO cohorts into high-risk and low-risk groups separately. Receiver operating characteristic (ROC) curves and independent prognostic factor analysis were carried out, and the prognostic efficacy was validated. The expression levels of ALOX12 in cell lines were examined. Inhibition of ALOX12 attenuated cell proliferation and migration in HEY cells. Moreover, the prognostic value of ALOX12 expression was examined in clinical samples of OC patients. Conclusion: This work constructed a novel ferroptosis-associated gene model. Furthermore, the clinical predictive role of ALOX12 was identified in OC patients, suggesting that ALOX12 might act as a potential prognostic tool and therapeutic target for OC patients. [ABSTRACT FROM AUTHOR]

At present, the traditional mode of research and development for mass-produced wetsuits usually requires repeated sample making and try-on evaluation, and performance cannot be predicted, monitored and evaluated in real time; this can lead to problems including low material utilization and production efficiency. In this study, real human body static and dynamic measurements, material properties and structure data are applied through 3D software to build an accurate virtual model, and new wetsuits are designed through simulation, optimization and evaluation. The static and dynamic fitting performance above and underwater is comprehensively evaluated in virtual and real environments, and it is proved that the virtual development mode can accurately and effectively guide the development and evaluation of wetsuits, and can meet personalized comfort and functional requirements today. This simulation evaluation method avoids repeated sample preparation, some unnecessary waste of materials and environmental pollution, and improves manufacturing efficiency. [ABSTRACT FROM AUTHOR]

In the alpine mountain area, the stability of the steep-slope permafrost subgrade is mainly affected by the temperature variation in the frozen soil layer at the base of the road. Under the conditions of climate warming and engineering-related disturbance, the cooling of the subgrade is an urgent problem to be solved in the field of construction. In this paper, the numerical calculation method, combined with data monitored in situ, is used to study the cooling effects of several subgrade cooling measures, in which the non-linear ventilation pipe and rubble layer are combined. The results show the following: (1) the temperature field of the steep-slope subgrade is clearly different in lateral terms—after 20 years of operation, the maximum difference in the melting depth between the left shoulder and the subgrade center is 3 m; (2) the maximum melting depth of the gravel subgrade center is 3.85 m, while the maximum difference in the melting depth between the left shoulder and the subgrade center is 2.3 m; (3) the cooling effect of the composite measures is noteworthy, and a −2.2 °C freezing area appears under the subgrade. [ABSTRACT FROM AUTHOR]

Background: Afatinib, a second-generation tyrosine kinase inhibitor (TKI), exerts its radiosensitive effects in nasopharyngeal carcinoma (NPC). However, the detailed mechanism of afatinib-mediated sensitivity to radiation is still obscure in NPC. Methods: Quantitative phosphorylated proteomics and bioinformatics analysis were performed to illustrate the global phosphoprotein changes. The activity of the CD44-Stat3 axis and Epithelial-Mesenchymal Transition (EMT)-linked markers were evaluated by Western blotting. Wound healing and transwell assays were used to determine the levels of cell migration upon afatinib combined IR treatment. Cell proliferation was tested by CCK-8 assay. A pharmacological agonist by IL-6 was applied to activate Stat3. The xenograft mouse model was treated with afatinib, radiation or a combination of afatinib and radiation to detect the radiosensitivity of afatinib in vivo. Results: In the present study, we discovered that afatinib triggered global protein phosphorylation alterations in NPC cells. Further, bioinformatics analysis indicated that afatinib inhibited the CD44-Stat3 signaling and subsequent EMT process. Moreover, functional assays demonstrated that afatinib combined radiation treatment remarkably impeded cell viability, migration, EMT process and CD44-Stat3 activity in vitro and in vivo. In addition, pharmacological stimulation of Stat3 rescued radiosensitivity and biological functions induced by afatinib in NPC cells. This suggested that afatinib reversed the EMT process by blocking the activity of the CD44-Stat3 axis. Conclusion: Collectively, this work identifies the molecular mechanism of afatinib as a radiation sensitizer, thus providing a potentially useful combination treatment and drug target for NPC radiosensitization. Our findings describe a new function of afatinib in radiosensitivity and cancer treatment. [ABSTRACT FROM AUTHOR]

Dynamic modeling and analysis are generally regarded as effective tools to investigate the vibration characteristics and fault mechanisms of planetary gear systems with a tooth crack fault. In actual gearboxes, the tooth crack is always a three-dimensional spatial surface, but it was usually simplified as a two-dimensional domain in most previous studies. In this paper, the tooth crack is modeled as a spatial shape that propagates along the crack depth, length and height directions simultaneously. Based on the potential energy principle, an improved analytical method is proposed to calculate the time-varying mesh stiffness (TVMS) of a planetary gear system with a spatial tooth crack. Furthermore, a coupled translational–torsional dynamic model is established for a planetary gear system including time-varying parameters and nonlinear factors. Numerical simulations are conducted to reveal the influences of the spatial crack propagation on the TVMS and vibration responses. In addition, an experimental study is carried out on a gear transmission test rig to verify the proposed analytical method and dynamic model. The mesh stiffness calculation method of the spatial cracked tooth and corresponding analysis results in this study might provide references to detect tooth crack faults in planetary gear systems. [ABSTRACT FROM AUTHOR]

As a special territory type, the farming–pastoral ecotone is facing challenges surrounding path creation and high-quality and sustainable development. Counties are not only an important spatial unit to promote high-quality development, but also an important part of the modernization of the national governance system. County-level development is the critical driving force of sustainable development and the breakthrough of governance modernization in the farming-pastoral ecotone. First, this study systematically reviews the progress of the farming–pastoral ecotone and high-quality development. Then, this study adopts the "Driving Forces-Pressure-State-Impact-Responses" (DPSIR) model and entropy method to construct an analysis framework. Third, taking Kulun county as a typical case, this study diagnoses the state of high-quality county-level development in the farming–pastoral ecotone. Finally, a governance mechanism of "idea-space-industry-people's livelihood-governance" has been proposed. This study provides theoretical guidance for the high-quality development of the farming–pastoral ecotone and enriches the body of knowledge regarding high-quality development. [ABSTRACT FROM AUTHOR]

The strictest water resources management (SWRM) policy is a critical policy to address China's severe water shortage and pollution problems, and aims to promote sustainable water development and water governance. Based on data mining from multiple sources, including policy text from the strictest water resource management policy from 2011 to 2021, the reports of major media websites, and the Baidu Index, this study used the ROST-CM6 text-analysis tool to analyze the policy content, public opinion, and public perception of the strictest water resources management policy quantitatively and visually. The results found that the policy text and public-opinion are given high attention to the water resources assessment, water control management, and water resources protection, but the policy text focuses on the macro level, and pays more attention to national development and long-term planning. The public opinion belongs to the micro level and is more economic, and there is a certain degree of media bias. With notable regional disparities, the general public's opinion of the harshest water resource management policy has been rising every year. This research adds to the global body of knowledge on water governance, and serves as a guide for Chinese and other governments looking to improve their water resource management strategies. [ABSTRACT FROM AUTHOR]

Rapid and accurate differentiation between live and dead cells is highly desirable for the evaluation of cell viability. Here, we report the application of the orange-emitting sulfur-doped organosilica nanodots (S-OSiNDs) for ultrafast (30 s), ultrasensitive (1 μg/mL), and universal staining of the dead bacterial, fungal, and mammalian cells but not the live ones, which satisfies the requirements of a fluorescent probe that can specifically stain the dead cells. We further verify that the fluorescence distribution range of S-OSiNDs (which are distributed in cytoplasm and nucleus) is much larger than that of the commercial dead/fixed cell/tissue staining dye RedDot2 (which is distributed in the nucleus) in terms of dead mammalian cell staining, indicating that S-OSiNDs possess a better staining effect of dead cells than RedDot2. Overall, S-OSiNDs can be used as a robust fluorescent probe for ultrafast and accurate discrimination between dead and live cells at a single cell level, which may find a variety of applications in the biomedical field. [ABSTRACT FROM AUTHOR]

The main gearbox is very important for the operation safety of helicopters, and the oil temperature reflects the health degree of the gearbox; therefore establishing an accurate oil temperature forecasting model is an important step for reliable fault detection. Firstly, in order to achieve accurate gearbox oil temperature forecasting, an improved deep deterministic policy gradient algorithm with a CNN–LSTM basic learner is proposed, which can excavate the complex relationship between oil temperature and working condition. Secondly, a reward incentive function is designed to accelerate the training time costs and to stabilize the model. Further, a variable variance exploration strategy is proposed to enable the agents of the model to fully explore the state space in the early training stage and to gradually converge in the training later stage. Thirdly, a multi-critics network structure is adopted to solve the problem of inaccurate Q-value estimation, which is the key to improving the prediction accuracy of the model. Finally, KDE is introduced to determine the fault threshold to judge whether the residual error is abnormal after EWMA processing. The experimental results show that the proposed model achieves higher prediction accuracy and shorter fault detection time costs. [ABSTRACT FROM AUTHOR]

At present, it is unclear how ergonomic factors of underwear affect men's work, lifestyle, physiology and psychology, and whether the demand factors of some special groups will affect the development of underwear. This study analyzed the underwear preferences of male groups working in Chinese and European universities from the perspective of ergonomics. The survey results confirm that ergonomic factors significantly affect men's preference for underwear, including subjective style preference and objective comfort. These problems come from style structure, materials, functional design and size standards. In general, this typical male group needs underwear with good support in standing posture and good comfort in sitting state. This study also provides important information and evidence for the analysis and prediction of group characteristics and demand-oriented product development. Designing underwear products according to consumer needs and avoiding waste of resources from inefficient development has a positive impact on the green development and recycling of textiles. [ABSTRACT FROM AUTHOR]

Promoting the coupling coordinated development between ecological protection and high-quality development is an important path to achieve sustainable regional development around the world. Taking Guizhou, China as a typical case, this study builds a comprehensive evaluation index system for ecological protection and high-quality development, and then uses the coupling coordination degree model to assess the spatiotemporal characteristics of ecological protection and high-quality development. According to the findings, Guizhou's ecological protection index and high-quality development index both exhibit an upward trend and a center-edge spatial pattern, but they also exhibit a "spatial misalignment relationship". The coupling coordination between ecological protection and high-quality development in Guizhou has shown a continuous growth trend from near-disorder to a primary coordination, with a spatial pattern of "central > western > eastern". This study enriches the knowledge body of sustainable development, helps Guizhou make decisions that are best for ecological protection and high-quality development, and is useful as a reference for other regions and countries. [ABSTRACT FROM AUTHOR]

Active packaging (AP) has been developed to improve the safety, quality and integrity of food, and minimise food waste, while its application in meat is scarce. This review aims to describe meat production and consumption culture in China and New Zealand to provide the context for packaging innovation requirements, focusing on the emerging opportunities for AP to be used for the improvement of the shelf-life of pre-rigor, aged, and frozen-thawed meat products. Sustainable polymers utilised in the manufacturing of AP, manufacturing techniques, the release mechanisms of actives, and legal and regulatory constraints are also discussed. Diverse market compositions and consumption cultures in China and New Zealand require different packaging solutions to extend the shelf-life of meat. AP containing antimicrobials, moisture regulating agents, and antioxidants may be used for pre-rigor, dry- and wet-aged products and in improving the quality and shelf-life of frozen-thawed meat. Further innovations using sustainably produced polymers for AP, along with incorporating active compounds of multiple functions for effectively improving meat quality and shelf-life are necessary. Challenges remain to resolve issues with scaling the technology to commercially relevant volumes as well as complying with the rigorous legal and regulatory constraints in various countries. [ABSTRACT FROM AUTHOR]

The strictest water resources management policy plays a critical role in response to the challenge of water shortage, water security, and sustainable water development in China. Despite the varied analyses of the strictest water resources management policy, the relations between the strictest water resources management policy and water use efficiency remain under-researched. This study uses an interval event-analysis method to assess the strictest water resources management policy's impact on water use efficiency in China based on data from 2007 to 2020. In addition, the study breaks down water use efficiency into eight indicators and divides the strictest water management policy into "pre-, middle, and post-" phases. The research results show the strictest water resources management policy has a significant positive effect on water use efficiency. Further research shows the total water consumption control system and water efficiency control system have the most significant effect. The strictest water resources management policy has a lasting impact on water use efficiency. This study contributes to the global knowledge body of water governance and provides a reference value for water policy decision-making and optimization in other countries. [ABSTRACT FROM AUTHOR]

Sustainable development is a common challenge for all global economies, and the assessment of the spatial distribution and development process of ecological resources is the basis of sustainable development. Considering the heterogeneity of regional ecological resource endowment, it is necessary to conduct a targeted assessment for different regions. In this study, we selected Guizhou Province, which is rich in ecological resources, but has a fragile environment, as our study area, and cultivated land, woodland, grassland, and water resources were selected as critical evaluation indicators. Notably, we applied Kernel density analysis methods, based on the remote sensing data of 2000, 2005, 2010, 2015, and 2020, to explore the spatiotemporal distribution characteristics and evolution pattern of regional ecological resources. The results indicated that the evolution of ecological resource development in Guizhou could be divided into three phases: degenerative (2000–2005), recovery (2005–2015), and development (2015–2020). The spatial distribution of Guizhou's ecological resource was shown to be heterogeneous in the north and south and more homogeneous in the middle regions. Guizhou has diverse land-use types, with obvious regional differences in land-use structure. Notably, even though the development of ecological resources in Guizhou has improved, the development and utilization degree of ecological resources is still low, and the ecological damage is serious. This study can be used as a scientific reference by policymakers and decisionmakers to develop new regulations for ecological resources protection and sustainable development in China. We suggest that different regions should adopt more detailed measures; particularly, it is important to establish a spatial governance system to promote ecological resources development in the context of local conditions. [ABSTRACT FROM AUTHOR]

Microalgae host varied microbial consortium harboring cross-kingdom interactions with fundamental ecological significance in aquatic ecosystems. Revealing the complex biofunctions of the cultivable bacteria of phycosphere microbiota is one vital basis for deeply understanding the mechanisms governing these dynamic associations. In this study, a new light-yellow pigmented bacterial strain LZ-28 was isolated from the highly-toxic and harmful algal bloom-forming dinoflagellate Alexandrium catenella LZT09. Collective phenotypic and genotypic profiles were obtained to confidently identify this strain as a new Mameliellaalba member. Comparative genomic analysis showed that strain LZ-28 shared highly similar functional features with other four marine algae-derived M. alba strains in spite of their distinctive isolation sources. Based on the bioactivity assaying, the mutual growth-promoting effects between bacterial strain LZ-28 and algal strain LZT09 were observed. After the culture conditions were optimized, strain LZ-28 demonstrated an extraordinary production ability for its bioflocculanting exopolysaccharides (EPS). Moreover, the portions of two monosaccharides glucose and fucose of the EPS were found to positively contribute to the bioflocculanting capacity. Therefore, the present study sheds light on the similar genomic features among the selected M. alba strains, and it also reveals the potential pharmaceutical, environmental and biotechnological implications of active EPS produced by this new Mameliella alba strain LZ-28 recovered from toxic bloom-forming marine dinoflagellate. [ABSTRACT FROM AUTHOR]

China's special economic zones have been hailed as a flagship of her policy on 'opening up' to the outside world, as well as the China-led global Belt and Road Initiative. In this paper, we conceptualize the internationalization of Chinese SEZs, and frame the genealogy and underpinning dynamics of China's international SEZs, both domestically and overseas. The paper critically probes the underlying logics of three parallel international developments of Chinese SEZs: (1) capital-oriented land expansion in the form of international SEZs combining both 'bring in' and 'going out', especially since BRI; (2) different industrial landscapes at various timelines and sites; (3) variegated transnational SEZs governance, bilateral central governments governance, marketized state or entrepreneurship state, or private ventures. We contribute to the SEZ land literature by delivering a novel framework which encompasses variegated internationalization trends among China's vast domestic and overseas SEZs. These internationalization trajectories in turn contour and entrench global variegated capitalism. [ABSTRACT FROM AUTHOR]

Featured Application: This study allows power converters to achieve high conversion efficiency due to the low parasitic inductance of multi-chip integration technology. Half-bridge modules with integrated GaN high electron mobility transistors (HEMTs) and driver dies were designed and fabricated in this research. Our design uses flip-chip technology for fabrication, instead of more generally applied wire bonding, to reduce parasitic inductance in both the driver-gate and drain-source loops. Modules were prepared using both methods and the double-pulse test was applied to evaluate and compare their switching characteristics. The gate voltage ( V g s ) waveform of the flip-chip module showed no overshoot during the turn-on period, and a small oscillation during the turn-off period. The probabilities of gate damage and false turn-on were greatly reduced. The inductance in the drain-source loop of the module was measured to be 3.4 nH. The rise and fall times of the drain voltage ( V d s ) were 12.9 and 5.8 ns, respectively, with an overshoot of only 4.8 V during the turn-off period under V d c = 100 V. These results indicate that the use of flip-chip technology along with the integration of GaN HEMTs with driver dies can effectively reduce the parasitic inductance and improve the switching performance of GaN half-bridge modules compared to wire bonding. [ABSTRACT FROM AUTHOR]

DDX6 (p54/RCK) is a human RNA helicase with central roles in mRNA decay and translation repression. To help our understanding of how DDX6 performs these multiple functions, we conducted the first unbiased, large-scale study to map the DDX6-centric protein-protein interactome using immunoprecipitation and mass spectrometry. Using DDX6 as bait, we identify a high-confidence and high-quality set of protein interaction partners which are enriched for functions in RNA metabolism and ribosomal proteins. The screen is highly specific, maximizing the number of true positives, as demonstrated by the validation of 81% (47/58) of the RNA-independent interactors through known functions and interactions. Importantly, we minimize the number of indirect interaction partners through use of a nuclease-based digestion to eliminate RNA. We describe eleven new interactors, including proteins involved in splicing which is an as-yet unknown role for DDX6. We validated and characterized in more detail the interaction of DDX6 with Nuclear fragile X mental retardation-interacting protein 2 (NUFIP2) and with two previously uncharacterized proteins, FAM195A and FAM195B (here referred to as granulin-1 and granulin-2, or GRAN1 and GRAN2). We show that NUFIP2, GRAN1, and GRAN2 are not P-body components, but re-localize to stress granules upon exposure to stress, suggesting a function in translation repression in the cellular stress response. Using a complementary analysis that resolved DDX6's multiple complex memberships, we further validated these interaction partners and the presence of splicing factors. As DDX6 also interacts with the E3 SUMO ligase TIF1α, we tested for and observed a significant enrichment of sumoylation amongst DDX6's interaction partners. Our results represent the most comprehensive screen for direct interaction partners of a key regulator of RNA life cycle and localization, highlighting new stress granule components and possible DDX6 functions--many of which are likely conserved across eukaryotes. [ABSTRACT FROM AUTHOR]

Our research aims to establish an evaluation framework and evaluate the sustainability of scientific research in universities. Based on the concept of Education for Sustainable Development and the function of scientific research activities, an evaluation framework was constructed including three dimensions: the sustainable trend of scientific research activity, research performance related to the topic of sustainable development, and sustainability of scientific research contributions. Descriptive analysis, Data Envelopment Analysis, and a Statistical Index Method were used to calculate the sustainability of scientific research of world-class universities in China. Results show that China's world-class universities published more articles related to sustainable development than the best-performing universities in the UK and USA. They make sustainable contributions to society through cultivating Ph.D. graduates, publishing research papers, and transforming science and technology. However, the sustainable trend of the scientific research of universities is still to be improved. The result of resource efficiency is relatively low, and attention should be paid to the waste of human and financial resources. In addition, universities should improve their ability to withstand external risks to minimize the influence of external public emergencies such as COVID-19. [ABSTRACT FROM AUTHOR]

Simple Summary: Immunotherapy is currently the backbone of new drug treatments for many cancer patients. CXC chemokine ligand 13 (CXCL13) is an important factor involved in recruiting immune cells that express CXC chemokine receptor type 5 (CXCR5) in the tumor microenvironment and serves as a key molecular determinant of tertiary lymphoid structure (TLS) formation. An increasing number of studies have identified the influence of CXCL13 on prognosis in patients with cancer, regardless of the use of immunotherapy treatment. However, no comprehensive reviews of the role of CXCL13 in cancer immunotherapy have been published to date. This review aims to provide an overview of the CXCL13/CXCR5 signaling axis to summarize its mechanisms of action in cancer cells and lymphocytes, in addition to effects on immunity and cancer pathobiology, and its potential as a biomarker for the response to cancer immunotherapy. Immune checkpoint inhibitors (ICIs), including antibodies that target programmed cell death protein 1 (PD-1), programmed death-ligand 1 (PD-L1), or cytotoxic T lymphocyte antigen 4 (CTLA4), represent some of the most important breakthroughs in new drug development for oncology therapy from the past decade. CXC chemokine ligand 13 (CXCL13) exclusively binds CXC chemokine receptor type 5 (CXCR5), which plays a critical role in immune cell recruitment and activation and the regulation of the adaptive immune response. CXCL13 is a key molecular determinant of the formation of tertiary lymphoid structures (TLSs), which are organized aggregates of T, B, and dendritic cells that participate in the adaptive antitumor immune response. CXCL13 may also serve as a prognostic and predictive factor, and the role played by CXCL13 in some ICI-responsive tumor types has gained intense interest. This review discusses how CXCL13/CXCR5 signaling modulates cancer and immune cells to promote lymphocyte infiltration, activation by tumor antigens, and differentiation to increase the antitumor immune response. We also summarize recent preclinical and clinical evidence regarding the ICI-therapeutic implications of targeting the CXCL13/CXCR5 axis and discuss the potential role of this signaling pathway in cancer immunotherapy. [ABSTRACT FROM AUTHOR]

The impact of device parameters, including AlN film thickness (hAlN), number of interdigital transducers (NIDT), and acoustic propagation direction, on the performance of c-plane AlN/sapphire-based SAW temperature sensors with an acoustic wavelength (λ) of 8 μm, was investigated. The results showed that resonant frequency (fr) decreased linearly, the quality factor (Q) decreased and the electromechanical coupling coefficient (K t 2) increased for all the sensors with temperature increasing from −50 to 250 °C. The temperature coefficients of frequency (TCFs) of sensors on AlN films with thicknesses of 0.8 and 1.2 μm were −65.57 and −62.49 ppm/°C, respectively, indicating that a reduction in hAlN/λ favored the improvement of TCF. The acoustic propagation direction and NIDT did not obviously impact the TCF of sensors, but they significantly influenced the Q and K t 2 of the sensors. At all temperatures measured, sensors along the a-direction exhibited higher fr, Q and K t 2 than those along the m-direction, and sensors with NIDT of 300 showed higher Q and K t 2 values than those with NIDT of 100 and 180. Moreover, the elastic stiffness of AlN was extracted by fitting coupling of modes (COM) model simulation to the experimental results of sensors along different directions considering Euler transformation of material parameter-tensors. The higher fr of the sensor along the a-direction than that along the m-direction can be attributed to its larger elastic stiffness c11, c22, c44, and c55 values. [ABSTRACT FROM AUTHOR]

This work studied the regulation of hole concentration and mobility in p-InGaN layers grown by metalorganic chemical vapor deposition (MOCVD) under an N-rich environment. By adjusting the growth temperature, the hole concentration can be controlled between 6 × 1017/cm3 and 3 × 1019/cm3 with adjustable hole mobility from 3 to 16 cm2/V.s. These p-InGaN layers can meet different requirements of devices for hole concentration and mobility. First-principles defect calculations indicate that the p-type doping of InGaN at the N-rich limiting condition mainly originated from Mg substituting In (MgIn). In contrast with the compensation of nitrogen vacancy in p-type InGaN grown in a Ga-rich environment, the holes in p-type InGaN grown in an N-rich environment were mainly compensated by interstitial Mg (Mgi), which has very low formation energy. [ABSTRACT FROM AUTHOR]

AlGaN/GaN E/D-mode GaN inverters are successfully fabricated on a 150-mm Si wafer. P-GaN gate technology is applied to be compatible with the commercial E-mode GaN power device technology platform and a systematic study of E/D-mode GaN inverters has been conducted with detail. The key electrical characters have been analyzed from room temperature (RT) to 200 °C. Small variations of the inverters are observed at different temperatures. The logic swing voltage of 2.91 V and 2.89 V are observed at RT and 200 °C at a supply voltage of 3 V. Correspondingly, low/high input noise margins of 0.78 V/1.67 V and 0.68 V/1.72 V are observed at RT and 200 °C. The inverters also demonstrate small rising edge time of the output signal. The results show great potential for GaN smart power integrated circuit (IC) application. [ABSTRACT FROM AUTHOR]

A flexible Ti/Cr/Ti multilayer strain gauge have been successfully developed based on polyimide substrate. The pure Ti metal strain gauge have shown the hysteresis phenomenon at the relationship between resistance and strain during tensile test. The experimental results of multilayer strain gauge show that adding Cr interlayer can improve the recovery and stability of the sensing electrode. When the interlayer Cr thickness was increased from 0 to 70 nm, the resistance decreased from 27 to 8.8 kΩ. The gauge factor (GF) value also decreased from 4.24 to 2.31 with the increase in the thickness of Cr interlayer from 30 to 70 nm, and the hysteresis phenomenon disappeared gradually. The multilayer Ti/Cr/Ti film has feasible application for strain sensor. [ABSTRACT FROM AUTHOR]

; (4) Deleting "in the IT industry" in the sentence of "Therefore, we used engagement in R&D work as the IV to test the robustness of our research results in the IT industry." in Section 4.4., " I Robustness Test i ", on page 12; (5) Deleting "in the IT industry" from the Table 9 caption: "IV 2SLS regression results in the IT industry" on page 12. Tables Table 9 IV 2SLS regression results in the IT industry. Reference 1 Wang C., Yang J., Cheng Z., Ni C. Postgraduate Education of Board Members and R&D Investment - Evidence from China. [Extracted from the article]

International collaboration is one of the effective ways to enhance the impact of scientific research papers. In this research, international research collaboration papers published by world-class universities in the field of humanities and social sciences from 2015 to 2019 were selected as the research object, and the effective enhancement of the impact of international research collaboration papers was found to not be dependent on expanding the scale of international research collaboration, but rather on selecting researchers with different international backgrounds and from high-level institutions for collaboration. It was also discovered that, in the field of humanities and social sciences, despite a relatively low proportion of international research collaboration papers being led by Chinese scholars, the Chinese research is characterized by a higher impact compared with the research led by non-Chinese scholars. In light of this, a series of proactive measures should be taken by China's world-class universities, such as actively participating in and initiating international collaboration, selecting high-level research collaborators, and attracting scholars from different countries to engage in research collaboration in the field of humanities and social sciences. [ABSTRACT FROM AUTHOR]

In this study, a novel method called selective proteolysis was applied to the glycinin component of soy protein isolate (SPI), and a degraded glycinin hydrolysate (DGH) was obtained. The effects of high-intensity ultrasound (HIU) treatment (20 kHz at 400 W, 0, 5, 20, and 40 min) on the physical, structural, and aggregation properties of DGH were investigated with the aim to reveal the influence of the selectively hydrolyzing glycinin component on the HIU treatment of soy protein. The effects of HIU on DGH and a control SPI (CSPI) were both time-dependent. HIU induced the formation of soluble aggregates in both samples at an early stage, while it dissociated these newly formed aggregates after a longer duration. Selectively hydrolyzing glycinin contributed to the soluble aggregation by exposing the compact protein structure and producing small protein fractions. The larger extent of hydrophobic interactions and disulfide bonds imparted a higher stability to the soluble protein aggregates formed in DGH. As a result, DGH displayed more ordered secondary structures, a higher solubility, and better gelling properties after the HIU treatment, especially at 20 min. The results of this study will be beneficial to the scientific community as well as industrial application. [ABSTRACT FROM AUTHOR]

AlGaN/GaN lateral Schottky barrier diodes (SBDs) with three different anode geometries (stripe, circular, and the conventional plane one) and different rows of anode trenches are fabricated and electrically characterized to study the influence of anode trench geometries. The SBDs with anode trenches exhibit the lower on-state resistance (RON) than that with the conventional plane one. It can be explained that the anode trenches made the Schottky metal directly contact to the 2DEG at the sidewall of the AlGaN/GaN interface, removing the AlGaN barrier layer in the conventional plane anode. In addition, the RON of the SBDs with circular trenches is smaller than that of the SBDs with stripe ones. Furthermore, the RON decreases with the increasing rows of anode trenches, which can be attributed to the increased contact area between the Schottky metal and the 2DEG. For the reverse characteristics, the anode trenches do not lead to performance degradation. The fabricated devices exhibit the low reverse current (IR, IR < 1 μA/mm), and the breakdown voltage (VBK) remains unchanged with different anode geometries. [ABSTRACT FROM AUTHOR]

Increasing research and development (R&D) investment has been a common strategy to advance the sustainable development of economy and competitiveness across the world. Instead of external determinants, exploring the influence of internal factors such as the characteristics of board members is an important topic, yet under-researched. This article aims to reveal whether a firm's R&D investment is related to the directors' postgraduate education experience. Further, we want to explore whether this relationship shows heterogeneity in different industrial environments. We analyzed information from a sample of 1374 listed companies in China using descriptive statistics, ordinary least square (OLS) regression and instrumental variable (IV) estimation, and came to the following conclusions: First, the percentage of directors with doctorates significantly increases the chance of investing R&D activities. Second, in the second industry, the higher the proportion of postgraduate education degree holder as directors in a firm, the more expenditure the firm invests in R&D activities. Yet, there is no such association in the third industry. Finally, if a capital-driven strategy is adopted, directors with a master's degree tend to reduce R&D investment in IT companies. Findings from this research not only enrich innovation management theory, upper echelon theory, and human capital theory, but also provide insights for corporate governance and national sustainable innovation. [ABSTRACT FROM AUTHOR]

Drag reduction by polymer is an important energy-saving technology, which can reduce pumping pressure or promote the flow rate of the pipelines transporting fluid. It has been widely applied to single-phase pipelines, such as oil pipelining, district heating systems, and firefighting. However, the engineering application of the drag reduction technology in two-phase flow systems has not been reported. The reason is an unrevealed complex mechanism of two-phase drag reduction and lack of numerical tools for mechanism study. Therefore, we aim to propose governing equations and numerical methods of direct numerical simulation (DNS) for two-phase gas-liquid drag-reducing flow and try to explain the reason for the two-phase drag reduction. Efficient interface tracking method—coupled volume-of-fluid and level set (VOSET) and typical polymer constitutive model Giesekus are combined in the momentum equation of the two-phase turbulent flow. Interface smoothing for conformation tensor induced by polymer is used to ensure numerical stability of the DNS. Special features and corresponding explanations of the two-phase gas-liquid drag-reducing flow are found based on DNS results. High shear in a high Reynolds number flow depresses the efficiency of the gas-liquid drag reduction, while a high concentration of polymer promotes the efficiency. To guarantee efficient drag reduction, it is better to use a high concentration of polymer drag-reducing agents (DRAs) for high shear flow. [ABSTRACT FROM AUTHOR]

Cancer cells can adapt to nutrient poor conditions by rewiring their metabolism and using alternate fuel sources. Identifying these adaptive metabolic pathways may provide novel targets for cancer therapy. Here, we identify a subset of non-small cell lung cancer (NSCLC) cell lines that survive in the absence of glucose by internalizing and metabolizing extracellular protein via macropinocytosis. Macropinocytosis is increased in these glucose independent cells, and is regulated by phosphoinositide 3-kinase (PI3K) activation of Rac-Pak signaling. Furthermore, inhibition of Rac-dependent macropinocytosis blocks glucose-independent proliferation. We find that degradation of internalized protein produces amino acids, including alanine, which generates TCA cycle and glycolytic intermediates in the absence of glucose. In this process, the conversion of alanine to pyruvate by alanine transaminase 2 (ALT2) is critical for survival during glucose starvation. Collectively, Rac driven macropinocytosis of extracellular protein is an adaptive metabolic pathway used by a subset of lung cancers to survive states of glucose deprivation, and may serve as a potential drug target for cancer therapy. [ABSTRACT FROM AUTHOR]

Funding and efficiency have always been important factors that restrict the sustainable development of tourism in developing countries. The PPP (Public Private Partnership) is an innovative model for addressing the above problems and is popular in the sustainable development of tourism around the world. The performance evaluation of tourism PPP is a key step towards promoting the PPP policy and sustainable development. In particular, the analysis of spatial effects that are easily overlooked will help to improve the measure of PPP performance. Based on tourism PPP projects in the PPP database of the Ministry of Finance in China, this paper analyses the spatial patterns and development characteristics of tourism PPP, identifies and measures the impact factors of tourism PPP by virtue of the GeoDetector method, and analyzes the function of PPP in tourism development and governance. The research shows that there are significant spatial disparities in the tourism PPP projects. Although tourism PPP is the result of interactions between multiple factors, what plays a critical role is the financial factor. PPP acts as a policy driver in tourism development in China. It corrects spatial mismatch between tourism resources and factors, eliminates space barriers and promotes the realization of spatial justice of tourism development. This study not only contributes to the improvement of China's PPP policy, but also has implications for PPP policies in other developing countries. [ABSTRACT FROM AUTHOR]

Background: Afatinib, a second-generation tyrosine kinase inhibitor (TKI), exerts its radiosensitive effects in nasopharyngeal carcinoma (NPC). However, the detailed mechanism of afatinib-mediated sensitivity to radiation is still obscure in NPC., Methods: Quantitative phosphorylated proteomics and bioinformatics analysis were performed to illustrate the global phosphoprotein changes. The activity of the CD44-Stat3 axis and Epithelial-Mesenchymal Transition (EMT)-linked markers were evaluated by Western blotting. Wound healing and transwell assays were used to determine the levels of cell migration upon afatinib combined IR treatment. Cell proliferation was tested by CCK-8 assay. A pharmacological agonist by IL-6 was applied to activate Stat3. The xenograft mouse model was treated with afatinib, radiation or a combination of afatinib and radiation to detect the radiosensitivity of afatinib in vivo., Results: In the present study, we discovered that afatinib triggered global protein phosphorylation alterations in NPC cells. Further, bioinformatics analysis indicated that afatinib inhibited the CD44-Stat3 signaling and subsequent EMT process. Moreover, functional assays demonstrated that afatinib combined radiation treatment remarkably impeded cell viability, migration, EMT process and CD44-Stat3 activity in vitro and in vivo. In addition, pharmacological stimulation of Stat3 rescued radiosensitivity and biological functions induced by afatinib in NPC cells. This suggested that afatinib reversed the EMT process by blocking the activity of the CD44-Stat3 axis., Conclusion: Collectively, this work identifies the molecular mechanism of afatinib as a radiation sensitizer, thus providing a potentially useful combination treatment and drug target for NPC radiosensitization. Our findings describe a new function of afatinib in radiosensitivity and cancer treatment.

The impact of device parameters, including AlN film thickness ( h AlN ), number of interdigital transducers ( N IDT ), and acoustic propagation direction, on the performance of c -plane AlN/sapphire-based SAW temperature sensors with an acoustic wavelength ( λ ) of 8 μm, was investigated. The results showed that resonant frequency ( f r ) decreased linearly, the quality factor ( Q ) decreased and the electromechanical coupling coefficient (Kt2) increased for all the sensors with temperature increasing from -50 to 250 °C. The temperature coefficients of frequency (TCFs) of sensors on AlN films with thicknesses of 0.8 and 1.2 μm were -65.57 and -62.49 ppm/°C, respectively, indicating that a reduction in h AlN / λ favored the improvement of TCF. The acoustic propagation direction and N IDT did not obviously impact the TCF of sensors, but they significantly influenced the Q and Kt2 of the sensors. At all temperatures measured, sensors along the a -direction exhibited higher f r , Q and Kt2 than those along the m -direction, and sensors with N IDT of 300 showed higher Q and Kt2 values than those with N IDT of 100 and 180. Moreover, the elastic stiffness of AlN was extracted by fitting coupling of modes (COM) model simulation to the experimental results of sensors along different directions considering Euler transformation of material parameter-tensors. The higher f r of the sensor along the a -direction than that along the m -direction can be attributed to its larger elastic stiffness c 11 , c 22 , c 44 , and c 55 values.