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Urothelial carcinoma (UC) is prevalent, especially in elderly males. The high rate of recurrence, treatment regime, and follow-up monitoring make UC a global health and economic burden. Arsenic is a ubiquitous toxicant that can be found in drinking water, and it is known that exposure to arsenic is associated with UC development. Around 25% of diagnosed UC cases are muscle-invasive (MIUC) which have poor prognosis and develop chemoresistance, especially if tumors are associated with squamous differentiation (SD). The immortalized UROtsa cell line is derived from normal human urothelium and our lab has malignantly transformed these cells using arsenite (As3+). These cells represent a basal subtype model of MIUC and the tumors derived from the As3+-transformed cells histologically and molecularly resemble clinical cases of the basal subtype of MIUC that have focal areas SD and expression of the basal keratins (KRT1, 5, 6, 14, and 16). Our previous data demonstrate that KRT6 protein expression correlates to areas of SD within the tumors. For this study, we performed a lentiviral knockdown of KRT6 in As3+-transformed UROtsa cells to evaluate the effects on morphology, gene/protein expression, growth, colony formation, and cisplatin sensitivity. The knockdown of KRT6 resulted in decreased expression of the basal keratins, decreased growth, decreased colony formation, and increased sensitivity to cisplatin, the standard treatment for MIUC. The results of this study suggest that KRT6 plays a role in UC cell growth and is an exploitable target to increase cisplatin sensitivity for MIUC tumors that may have developed resistance to cisplatin treatment. [ABSTRACT FROM AUTHOR]

This study aimed to determine the relationship between the concentration of heavy metals in drinking water and the number of type 1 diabetes mellitus (T1DM) cases in two regions of Poland. The number of births in 2015-16 in Pomeranian Voivodeship was 50,461, while the number of new T1DM cases was 219. In Lublin Voivodeship, the number of births in the same period reached 39,381, and the number of new T1DM cases was 221. The incidence of T1DM, calculated per 100 live births, amounted to 0.43 and 0.56 in Pomeranian and Lublin. The statistical analysis of collected data proved that the number of new T1DM cases in Pomeranian Voivodeship is correlated to the concentrations of selenium (Se; p < 0.0001), lead (Pb; p < 0.00001), cadmium (Cd; p < 0.00001), zinc (Zn; p < 0.00001) and arsenic (As; p = 0.00001). In the case of Lublin Voivodeship, the number of new T1DM cases was correlated to the concentrations of Se (p = 0.0000001), Pb (p < 0.000001), Cd (p = 0.0000001) and Zn (p < 0.00001) in drinking water. No correlation was found between the number of new T1DM cases and arsenic concentration in the drinking water samples from Lublin Voivodeship. The results indicated that environmental exposure to specific heavy metals may contribute to the risk of T1DM, underscoring the need for stringent environmental controls and public health policies to mitigate these risks. [ABSTRACT FROM AUTHOR]

Carbon dots (CDs) derived from mandarin peel biochar (MBC) at different pyrolysis temperatures (200, 400, 600, and 800 °C) have been synthesized and characterized. This high-value transformation of waste materials into fluorescent nanoprobes for environmental monitoring represents a step forward towards a circular economy. In this itinerary, CDs produced via one-pot hydrothermal synthesis were utilized for the detection of copper (II) ions. The study looked at the spectroscopic features of biochar-derived CDs. The selectivity of CDs obtained from biochar following carbonization at 400 °C (MBC400-CDs towards various heavy metal ions resulted in considerable fluorescence quenching with copper (II) ions, showcasing their potential as selective detectors. Transmission electron microscopic (TEM) analysis validated the MBC-CDs' consistent spherical shape, with a particle size of <3 nm. The Plackett–Burman Design (PBD) was used to study three elements that influence the F0/F ratio, with the best ratio obtained with a pH of 10, for 10 min, and an aqueous reaction medium. Cu (II) was detected over a dynamic range of 4.9–197.5 μM and limit of detection (LOD) of 0.01 μM. Validation testing proved the accuracy and precision for evaluating tap and mountain waters with great selectivity and no interference from coexisting metal ions. [ABSTRACT FROM AUTHOR]

Global concern over arsenic contamination in drinking water necessitates innovative and sustainable remediation technologies. This study evaluates the adsorption performance of Fe–Mn binary oxide (FMBO) nanocomposites developed by coating polyethylene (PE) and polyethylene terephthalate (PET) with FMBO for the removal of As(III) and As(V) from water. Adsorption kinetics were rapid, with equilibrium achieved within 1–4 h depending on the material and pH. PET-FMBO and FMBO exhibited faster rates and higher arsenic removal (up to 96%) than PE-FMBO. Maximum As(III) adsorption capacities ranged from 4.76 to 5.75 mg/g for PE-FMBO, 7.2 to 12.0 mg/g for PET-FMBO, and up to 20.8 mg/g for FMBO, while capacities for As(V) ranged from 5.20 to 5.60 mg/g, 7.63 to 18.4 mg/g, and up to 46.2 mg/g, respectively. The results of the Dubinin–Radushkevich isotherm model, with free energy (Ea) values exceeding 16 kJ/mol, suggest chemisorption is the dominant mechanism, which is supported by the kinetics data. Given the effective removal of As(III), chemisorption likely proceeds through ligand exchange during the Mn oxide-mediated oxidation of As(III) and complexation with hydroxyl groups on the nanocomposite. These findings highlight the strong potential of Fe–Mn polymer nanocomposites, particularly PET-FMBO, for efficient arsenic removal during practical water treatment applications. [ABSTRACT FROM AUTHOR]

The production and consumption of plastic bottled water have several negative environmental impacts worldwide. To identify the barriers and motivations for drinking tap and bottled water, we conducted a nationally representative questionnaire survey among 3411 respondents in the Czech Republic in 2022. People aged 18–34 are moderate consumers of bottled water and very frequent consumers of tap water. Bottled water consumption tends to be less frequent among people with a higher education, while tap water consumption is less frequent among people with lower incomes. The most important factors that explain the frequency of drinking bottled and tap water are taste perception, health concerns and habit. Health concerns about tap water and the unpleasant taste of tap water increase the consumption of bottled water. People with a strong habit of drinking tap water are less likely to consume bottled water. The constructs from the theory of planned behaviour were statistically significant. The results can guide decision-makers in promoting tap water to consumers. To encourage tap water drinking, we suggest measures to increase the availability of tap water in public places in conjunction with campaigns targeting the taste and health perception, as well as the habit, of drinking tap water. [ABSTRACT FROM AUTHOR]

This study focused on fluoride (F−) contamination of water sources in Bahimi village, Cameroon. After the first investigation, results revealed that all water samples collected had elevated concentrations of fluoride ions (2.3 ± 0.1) mg/L to (4.5 ± 0.2) mg/L above the WHO guidelines (less than 1.5 mg/L). To mitigate the F− levels, the use of bone char (BC) as an adsorbent material was proposed and its performance was tested. BC was prepared from bovine bones at different calcination temperatures (350 °C, 450 °C, 550 °C and 650 °C) and residence times (1 h and 2 h). The prepared materials were characterized in detail by SEM/EDS, BET, FTIR, and XRD. The BET findings indicated that the surface area of BC samples decreased with increasing calcination temperature and residence time. At a lower heating temperature and holding time (350 °C, 1 h), the prepared BC exhibited a higher specific surface area (112.3 ± 0.3) m2/g and adsorption capacity for F− in the sampled water. Also, the batch adsorption experiments showed that the optimized adsorbent dose of 8 g/L facilitates the reduction in the F− level of the sampled water below the acceptable limit level (1.5 mg/L) within 5 min of treatment. The presence of Ca2+ and Mg2+ in natural water has a positive effect on the removal of F− in BC resulting in a high adsorption performance range of (72.5 ± 1.4)% to (80.3 ± 0.6)%. It was found that the adsorption of Ca2+ on the BC surface occurs via cation exchange with Na+. However, an increase in dissolved organic carbon (DOC) in the treated water limited the application of BC. Overall, the study presented a cost-effective adsorbent for the removal of this recalcitrant ion in the water source. [ABSTRACT FROM AUTHOR]

Access to clean and safe drinking water is a fundamental human right. Despite global efforts, including the UN's "Water for Life" program, a significant portion of the population in developing countries, including Somaliland, continues to rely on unimproved water sources. These unimproved sources contribute to poor health outcomes, particularly for children. This study aimed to investigate the factors associated with the use of unimproved drinking water sources in Somaliland by employing supervised machine learning models to predict patterns and determinants based on data from the 2020 Somaliland Demographic and Health Survey (SHDS). Secondary data from SHDS 2020 were used, encompassing 8384 households across Somaliland. A multilevel logistic regression model was applied to analyze the individual- and community-level factors influencing the use of unimproved water sources. In addition, machine learning models, including logistic regression, decision tree, random forest, support vector machine (SVM), and K-nearest neighbor (KNN), were compared in terms of accuracy, sensitivity, specificity, and other metrics using cross-validation techniques. This study uses supervised machine learning models to analyze unimproved drinking water sources in Somaliland, providing data-driven insights into the complex determinants of water access. This enhances predictive accuracy and informs targeted interventions, offering a robust framework for addressing water-related public health issues in Somaliland. The analysis identified key determinants of unimproved water source usage, including socioeconomic status, education, region, and household characteristics. The random forest model performed the best with an accuracy of 93.57% and an area under the curve (AUC) score of 98%. Decision tree and KNN also exhibited strong performance, while SVM had the lowest predictive accuracy. This study highlights the role of socioeconomic and community factors in determining access to clean drinking water in Somali Land. Factors such as age, education, gender, household wealth, media access, urban or rural residence, poverty level, and literacy level significantly influenced access. Local policies and resource availability also contribute to variations in access. These findings suggest that targeted interventions aimed at improving education, infrastructure, and community water management practices can significantly reduce reliance on unimproved water sources and improve the overall public health. [ABSTRACT FROM AUTHOR]

This study provides a detailed numerical assessment of the productivity of single-slope solar stills across key cities in the Caspian region, including Aktau, Atyrau, Astrakhan, Makhachkala, Baku, Tehran, and Turkmenbashi. Employing a mathematical model based on heat balance equations and the Fourth Order Runge–Kutta method, we simulated the distillation process under various climatic conditions. The results reveal that productivity is significantly influenced by geographic location and local meteorological conditions. Tehran demonstrated the highest productivity across all seasons, with values of 1.75 × 103 kg/(m2·year) and an efficiency of 0.53, due to its optimal solar irradiation and ambient temperature. In contrast, Atyrau and Makhachkala exhibited lower productivity, particularly in colder months, highlighting the effect of ambient temperature on solar still efficiency. The analysis identified the optimal water depth at 2 cm and insulation thickness between 4 and 9 cm for enhancing productivity in continental climates like Aktau. Additionally, the lowest cost of distilled water was USD 0.024 per kilogram in Baku. These findings align with the existing literature, validating the numerical model's accuracy. Future research will explore integrating solar stills with other renewable and fossil fuel-based technologies. [ABSTRACT FROM AUTHOR]

Groundwater is becoming increasingly important as surface water is decreasing and becoming more and more polluted. In particular, rural areas in the arid region of Central Asia face problems with both water quantity and quality. In view of this, we investigated the drinking water quality in the Maysky district in the Pavlodar region, Kazakhstan. The organoleptic properties, together with microbiological indicators, as well as organic and inorganic substances of drinking water before and after treatment, and tap water were studied and compared to recommended levels. The bacteriological indicators of the drinking water, especially, showed that the water represents health risks since the presence of bacteria of the genus Pseudomonas aeruginosa was confirmed. Water treatment reduced the total microbial count (TMC) indicator by 3.6 times. However, TMC still exceeded permissible levels in the tap water, indicating that the drinking water is sanitary and epidemiologically not acceptable. Pathogenic contamination of drinking water can severely affect weaker individuals and children. It has been estimated that the infant mortality rate in Kazakhstan is six times higher as compared to the EU and less than 30% of Kazakhstan's population have access to safe water. Also, 50% of the population drink water that does not comply with the international standards, e.g., bacteriological levels. Thus, it is important to continuously monitor the groundwater quality to minimize health risks and work towards access to safe drinking water, in line with the UN SDGs. [ABSTRACT FROM AUTHOR]

Drinking water distribution systems (DWDSs) may be contaminated to various degrees when different microorganisms attach to the pipe walls. Understanding the characteristics of biofilms on pipe walls can help prevent and control microbial contamination in DWDSs. The biofilm formation, interspecific interactions, and chlorine resistance of 10 dual-species biofilms in polyethylene (PE) and cast iron (CI) pipes were investigated in this paper. The biofilm biomass (heterotrophic bacterial plate count and crystal violet) of dual species in CI pipes is significantly higher than that in PE pipes, but the biofilm activity in CI pipes is significantly lower than that in PE pipes. The interspecific interaction of Sphingomonas-containing group presented synergistic or neutral relationship in PE pipes, whereas the interspecific interaction of the Acidovorax-containing group showed a competitive relationship in CI pipes. Although interspecific relationships may help bacteria resist chlorine, the chlorine resistance was more reliant on dual-species groups and pipe materials. In CI pipes, the Microbacterium containing biofilm groups showed better chlorine resistance, whereas in PE pipes, most biofilm groups with Bacillus exhibited better chlorine resistance. The biofilm groups with more extracellular polymeric substance (EPS) secretion showed stronger chlorine resistance. The biofilm in the PE pipe is mainly protected by EPS, while both EPS and corrosion products shield the biofilms within CI pipe. These results supported that dual-species biofilms are affected by pipe materials and interspecific interactions and provided some ideas for microbial control in two typical pipe materials. [ABSTRACT FROM AUTHOR]

(1) Mercury (Hg) is a persistent, ubiquitous contaminant that readily biomagnifies into higher trophic level species in aquatic environments across the globe. It is crucial to understand the movement of environmentally relevant concentrations of Hg in impacted freshwater streams to minimize risks to ecological and human health. (2) The bioconcentration kinetics of aqueous Hg exposure (20, 100, and 200 ng/L) in the invasive Asian Clam, Corbicula fluminea, were measured. A toxicokinetic model, the first parameterized for Hg accumulation in freshwater clams, was developed to estimate uptake and efflux parameters and compared to previous parameter values estimated for other mollusk species. (3) Results demonstrated that even at low Hg concentrations, Corbicula record signals of contamination through bioconcentration, and both direct measurement and toxicokinetic models demonstrate large Hg bioconcentration factors (as high as 1.34 × 105 mL/g dry tissue), similar to partitioning coefficients seen in engineered Hg sorbents. (4) Our study found that Corbicula accumulated Hg at aqueous concentrations relevant to impacted streams, but well below regulatory drinking water limits, demonstrating their utility as a sensitive sentinel species and potential bioremediator. [ABSTRACT FROM AUTHOR]

The aim of the study was to assess the effect of water infusion of dried and crushed ground ivy (GH) on the fermentation properties of wheat flour (WF), farinographic properties of flour and dough (WD) and the quality of the obtained bread. In the tested systems, tap water was replaced with water infusion with GH (m/v) at a concentration of 1% (A), 3% (B) and 5% (C). As part of the research methodology, the fermentation properties of flour and rheological properties of dough were assessed using a farinograph, and bread was obtained using a single-phase method using yeast and its quality was assessed. As part of this, the antioxidant potential and the profile and level of polyphenol content were determined. It was shown that replacing water with GH infusions shortened the total fermentation time of the dough and reduced the fermentation capacity of the dough. In the farinographic evaluation, an increase in flour water absorption (54.0–57.0%), dough development time (2.3–7.6 min), dough stability and softening were observed with an increase in the concentration of the added GH infusion. In turn, the volume of the loaf and the specific volume of the bread decreased with increasing the concentration of the GH infusion. The bread crumb darkened, and the elasticity and chewiness of the crumb decreased in relation to the control sample. In turn, the presence of GH infusion did not significantly affect the hardness of the crumb. As the concentration of the added GH infusion increased, an increase in the antioxidant potential of bread and the content of polyphenols and flavonoids was observed, and the UPLC-PDA-MS/MS analysis allowed the identification of 11 polyphenols in the bread. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the effect of irrigation depths (IDs) with brackish water and levels of organic fertilizer (OF) on the morphological aspects, production, chemical composition and in vitro digestibility of cactus pear grown in a semiarid region. This experiment was conducted in an area already established for 2 years with cactus pear [Opuntia stricta (Haw.)] and started 18 months after the standardization cut. This was a 5 × 4 factorial design of five irrigation depths (0, 12.5, 25, 37.5 and 50% evapotranspiration—ETo) and four levels of organic fertilizer (0, 15, 30, and 45 megagram per hectare—Mg/ha), with four replications. The water used in the experiment was classified with high salinity and low sodium content (C3S1), and presented an electrical conductivity of 1.73 dS/m. There was no effect of the ID × OF interaction on the morphological and productive characteristics of cactus pear (p > 0.05); however, the ID × OF interaction promoted effects on ether extract, crude protein, neutral detergent insoluble protein, neutral detergent insoluble ash, total carbohydrates and non-fiber carbohydrates (p < 0.05). The ID and OF levels separately influenced the productive, morphological and nutritional characteristics of cactus pear. Under experimental conditions, we recommend the use of organic fertilizer at a level up to 45 Mg/ha, which is associated with the use of lower brackish water levels for the cultivation of cactus pear. This study's findings provide new insights into reducing the use of potable water in crop irrigation for dryland regions and other regions. [ABSTRACT FROM AUTHOR]

Cyanobacterial blooms have become a serious water pollution problem in many parts of the world, and the monitoring and study of the impacts of biotoxins on human health are of vital importance. In this study, the contents of microcystin-LR, 2-methylisoborneol, and geosmin were measured in water and sediment samples from Nanwan Reservoir, China, by means of bimonthly sampling between February and December 2023. The physicochemical and hydrochemical factors and phytoplankton dynamics in the reservoir were also investigated. The results showed that the overall mean concentration of microcystin-LR (0.729 μg/L) in summer approached the guiding standard (1 μg/L) set by the WHO for drinking water. Furthermore, the content of 2-methylisoborneol (143.5 ng/L) was 14 times higher than the national standard (10 ng/L). The results of laboratory cultures showed that lower light levels and medium temperatures were suitable for the growth of Microcystis and Planktothricoides but higher temperatures promoted the synthesis and release of microcystin-LR and 2-methylisoborneol. In addition, the results of co-cultures showed that the growth of Planktothricoides was inhibited by Microcystis. Our results suggest that cyanobacterial bloom and the presence of the metabolites 2-methylisoborneol and microcystin-LR can decrease the drinking water quality of Nanwan Reservoir. [ABSTRACT FROM AUTHOR]

A new molecularly imprinted polymer (MIP)-based disposable electrochemical sensor for dipyridamole (DIP) determination was obtained. The sensor was rapidly prepared by potentiodynamic electrochemical polymerization on a pencil graphite electrode (PGE) using curcumin (CUR) as a functional monomer and DIP as a template molecule. After the optimization of the conditions (pH, monomer–template ratio, scan rate, number of cyclic voltammetric cycles applied in the electro-polymerization process and extraction time of the template molecule) for MIP formation, DIP voltammetric behavior at the modified electrode (MIP_PGE) was investigated. DIP oxidation took place in a pH-dependent, irreversible mixed diffusion-adsorption controlled process. Differential pulse voltammetry (DPV) and adsorptive stripping differential pulse voltammetry (AdSDPV) were used to quantify DIP from pharmaceutical and tap water samples. Under optimized conditions (Britton–Robinson buffer at pH = 3.29), the obtained linear ranges were 5.00 × 10−8–1.00 × 10−5 mol/L and 5.00 × 10−9–1.00 × 10−7 mol/L DIP for DPV and AdSDPV, respectively. The limits of detection of the methods were 1.47 × 10−8 mol/L for DPV and 3.96 × 10−9 mol/L DIP for AdSDPV. [ABSTRACT FROM AUTHOR]

Approximately two billion people worldwide lack access to clean drinking water, negatively impacting national security, hygiene, and agriculture. Atmospheric water harvesting (AWH) is the conversion of ambient humidity into clean water; however, conventional dehumidification is energy-intensive. Improvement in AWH may be achieved with elastocaloric cooling, using temperature-sensitive materials in active thermoregulation. Potential benefits, compared to conventional desiccant wheel designs, include substantial reductions in energy use, size, and complexity. A nickel–titanium (NiTi) elastocaloric water harvester was designed and compared with a desiccant wheel design under controlled conditions of relative humidity, air volume, and power. In a 30 min interval, the NiTi device harvested more water on average at 0.18 ± 0.027 mL/WH, compared to the 0.1567 ± 0.023 mL/WH of the desiccant wheel harvester. Moreover, the NiTi harvester required half the power input and was thermoregulated more efficiently. Future work will focus on mechanical design parameter optimization. Elastocaloric cooling is a promising advancement in dehumidification, making AWH more economical and feasible. [ABSTRACT FROM AUTHOR]

Water quality is a fundamental aspect of public health and environmental sustainability. In rural areas, the physicochemical and microbiological quality of drinking water depends not only on hydrogeological conditions but also on anthropogenic activities carried out on the surface of the basin. This study aimed to identify natural and anthropogenic influences related to the quality of drinking water in rural areas of southern Chile. In order to perform this, six rural drinking water systems were evaluated. A total of two types of catchment sources (groundwater and surface water) that were located in a longitudinal gradient were used, where coverage and sequences of rocks and soils could be differentiated. The results show that the water delivered by the majority of rural drinking water systems studied was of good quality, meeting the standards of Chilean and international regulations. No fecal coliforms or Escherichia coli were recorded. In addition, we recorded that turbidity, color, pH, concentration of total dissolved solids and fecal coliforms showed significant differences between groundwater and surface water. We also recorded that in two groundwater systems, iron and manganese levels slightly exceeded the regulations, endangering the acceptability of the water. These increases can be related to the natural origins of the metals, linked to the presence of oxides in Andisol- and Utisol-type volcanic soils. [ABSTRACT FROM AUTHOR]

Drinking water temperatures are expected to increase in the Netherlands due to climate change and the installation of district heating networks as part of the energy transition. To determine effective measures to prevent undesirable temperature increases in drinking water, a model was developed. This model describes the temperature in the drinking water distribution network as a result of the transfer of heat from the climate and above and underground heat sources through the soil. The model consists of two coupled applications. The extended soil temperature model (STM+) describes the soil temperatures using a two-dimensional finite element method that includes a drinking water pipe and two hot water pipes coupled with a micrometeorology model. The extended water temperature model (WTM+) describes the drinking water temperature as a function of the surrounding soil temperature (the boundary temperature resulting from the STM+), the thermal sphere of influence where the drinking water temperature influences the soil temperature, and the hydraulics in the drinking water network. Both models are validated with field measurements. This study describes the WTM+. Previous models did not consider the cooling effect of the drinking water on the surrounding soil, which led to an overestimation of the boundary temperature and how quickly the drinking water temperature reaches this boundary temperature. The field measurements show the improved accuracy of the WTM+ when considering one to two times the radius of the drinking water pipe as the thermal sphere of influence around the pipe. [ABSTRACT FROM AUTHOR]

Water quality concerns the physical, chemical, and biological factors that could negatively impact human health through its consumption, potentially causing infectious and chronic diseases due to immediate or prolonged exposure. In this context, the objective of this study is to identify diseases that are correlated with the quality of drinking water according to the literature. A systematic review was carried out considering academic and scientific documents from the last 6 years, including peer-reviewed research articles, books, and technical documents, such as standards and regulations related to public health and water quality. Subsequently, these results were applied to a case study from Tonosí (a district in Panama), where a drinking water quality assessment project was developed over the past two years including physicochemical, biological, inorganic chemical, and organic chemical analyses on drinking water during the rainy and dry seasons. Forty-five documents were obtained from the literature review and are presented in tables relating to diseases and water quality parameters. Based on the drinking water quality assessment results from Tonosí, the levels above and below the permissible range—according to the DGNTI-COPANIT 21-2019 Technical Regulation adopted by Panama as a drinking water quality standard—and the diseases associated with the parameters evaluated (in accordance with the literature review) are presented. The results show that there is a possible relationship between some of the water quality parameters and cases of gastrointestinal diseases in the area; however, more in-depth research and statistics at the national level are needed on the health of the population. [ABSTRACT FROM AUTHOR]

Forests are critical source regions of high-quality drinking water but forest disturbances such as harvesting can alter stream dissolved organic carbon (DOC) concentrations and influence source water treatability. Most stream DOC-centric forest harvesting impact studies report on effects <10 years post-harvest; less is known about the legacy effects of forest harvesting on stream DOC. Here, inter- and intra-catchment variability in stream DOC concentration and export were evaluated in two northern hardwood-dominated headwater catchments (unharvested reference and 24 years post-clearcut). The relationship between stream DOC and the concentration, spatial distribution, and hydrologic connectivity of hillslope solute pool DOC was investigated. Stream DOC concentrations in the legacy clearcut catchment exceeded those in the reference catchment for all flow conditions. Inter-catchment differences in DOC export were inconsistent. Hillslope solute pool DOC concentrations decreased with soil depth but were not significantly different between catchments. Concentration–discharge regression analysis indicated that DOC was primarily transport-limited (flushing) in both catchments. Aqueous potassium silica molar ratio data indicate the influence of groundwater on stream chemistry and streamflow was similar in both catchments. Results suggest that while clearcut harvesting can have detectable decadal-scale effects on stream DOC concentrations in northern hardwood-dominated headwater catchments, the effects are limited and likely do not pose a reasonable threat to downstream drinking water treatment operations. [ABSTRACT FROM AUTHOR]

The coagulation and flocculation properties of chitosan, an organic biopolymer derived from chitin, have been researched as an alternative to synthetic polymers and inorganic metal salt coagulants currently used in water and wastewater treatment. In an effort to encourage further research into the practical uses of chitosan as green chemistry in water and wastewater treatment and to promote the efficacious removal of microbial contaminants in drinking and wastewater, we have summarized the current state of research pertaining to the treatment of microorganisms in water and wastewater. A search of PubMed revealed 720 possible titles and abstracts, of which 44 full-text articles were identified as matching the eligibility criteria for inclusion in this systematic review. Results are presented based on the type of water matrix treated (i.e., drinking water, wastewater, and recreational waters) and a summary table providing details on the types and forms of chitosan utilized and the treatment mechanisms and processes described in the study. We find chitosan to be an effective coagulant, flocculant, and adsorbent for removing microbes from water and wastewater; some modified forms of chitosan can inactivate microbes and achieve disinfection, such as those containing metals like silver and antimicrobial chemicals like quaternary ammonium compounds or other strong oxidants, and use with filtration or electrochemical processes can achieve extensive reductions in microbes to meet performance targets of the World Health Organization. [ABSTRACT FROM AUTHOR]

Hexavalent chromium (Cr[VI]) is a widespread environmental pollutant in air and water that is primarily attributed to industrial pollution. The current maximum contaminant levels (MCLs) for drinking water from the World Health Organization and the U.S. Environmental Protection Agency (0.05 and 0.1 mg/L, respectively) were set based on contact dermatitis and warrant further toxicological investigation. While Cr(VI) is neurotoxic and accumulates in the brain, most animal studies only report whole-brain Cr, leaving large knowledge gaps. Few studies consider differences between ages or sexes, and fewer consider essential metal dyshomeostasis. We sought to investigate where Cr accumulates in the brain, considering sex and age differences, following a 90-day drinking water exposure to current MCLs. Here, we report Cr levels in six brain regions of rats exposed to drinking water Cr(VI). We observed Cr only accumulated in the hippocampus, and only in older females. We further assessed changes to essential metals in the hippocampus, observing opposite effects across sexes and between young rats compared to older rats. In sum, our data indicate drinking water Cr(VI) selectively targeted the hippocampus, with geriatric females accumulating the most Cr, and induced significant essential metal dyshomeostasis even in tissues lacking evident Cr accumulation. [ABSTRACT FROM AUTHOR]

With the rapid development of nuclear energy, the contamination of environmental water systems by uranium has become a significant threat to human health. To efficiently remove uranium from these systems, three types of silica-based polyamine resins—SiPMA-DETA (SiPMA: silica/poly methyl acrylate; DETA: diethylenetriamine), SiPMA-TETA (TETA: triethylenetetramine), and SiPMA-TEPA (TEPA: tetraethylenepentamine)—were successfully prepared, characterized, and evaluated in batch experiments. Characterization results showed that the silica-based polyamine resins were successfully prepared, and they exhibited a uniform shape and high specific surface area. SiPMA-DETA, SiPMA-TETA, and SiPMA-TEPA had nitrogen contents of 4.08%, 3.72%, and 4.26%, respectively. Batch experiments indicated that these adsorbents could efficiently remove uranium from aqueous solutions with a pH of 5–9. The adsorption kinetics of U(VI) were consistent with the pseudo-second-order model, indicating that the adsorption process was chemisorption and that adsorption equilibrium was achieved within 10 min. SiPMA-TEPA, with the longest polyamine chain, exhibited the highest adsorption capacity (>198.95 mg/g), while SiPMA-DETA, with the shortest polyamine chain, demonstrated the highest U(VI) adsorption efficiency (83%) with 100 mM Na2SO4. SiPMA-TEPA still removed over 90% of U(VI) from river water and tap water. The spectral analysis revealed that the N-containing functional groups on the ligand were bound to anionic uranium–carbonate species and possibly contributed to the adsorption efficiency. In general, this work presents three effective adsorbents for removing uranium from environmental water systems and thus significantly contributes to the field of environmental protection. [ABSTRACT FROM AUTHOR]

Background: The pollution of organophosphate flame retardants (OPFRs) is of global concern, but the site-specific data of OPFR concentrations in drinking water are scarce for many areas of the world outside of Europe and the US. This study aimed to investigate the occurrence and profiles of OPFRs in the tap water treatment and delivery process in Shanghai. Methods: In total, 106 samples were analyzed for 10 OPFRs, which were collected periodically from monitoring points of drinking water treatment plants and piped water between November 2021 and July 2023. The average daily doses of OPFRs through the ingestion of tap water were calculated by multiplying nominal volumes of water ingestion rates with the measured concentrations of OPFRs. Hazard quotients, the hazard index, and the carcinogenic risks of OPFRs via drinking water were used to estimate the health risks. Results: Tributyl phosphate (TBP), tris(2-chloroethyl) phosphate (TCEP), and tris (1-chloro-2-propyl) phosphate (TCIPP) were found in >90% of the tap water samples, whereas triethyl phosphate (TEP) and tris (2,3-dibromopropyl) phosphate (TDBPP) were not found in any samples. The concentrations of Σ10OPFRs were found at part-per-trillion ranges, with average concentrations that ranged from 86.0 ng/L in February 2023 (dry season) to 218 ng/L in July 2022 (wet season). TCIPP was the most abundant compound among the investigated OPFRs. The average daily dose of Σ10OPFRs via the ingestion of tap water was up to 20.4 ng/kg body weight/day. The hazard quotients of OPFRs through drinking water were in the range of 10−5–10−4, indicating low risk levels. Moreover, the hazard index of OPFRs indicated that the risk for children (2 × 10−4) was higher than adults (7 × 10−5). Conclusion: Tap water intake may be an important source of OPFRs exposure. But the risk of OPFRs for local residents is at a low level through drinking water. [ABSTRACT FROM AUTHOR]

This study introduces a novel approach for transforming the cationic surfactant, cetyltrimethylammonium bromide (CTAB), into fluorescent carbon dots (CDs) capable of detecting cadmium ions at ultralow concentrations. The current approach involves preparing CDs through hydrothermal process at various pH levels. The CDs synthesized using pH 10, CTAB-CDs-10, exhibited the highest fluorescence quantum yield (QY), 14.22%. UV-Vis spectroscopy helped identify specific peaks between 210 and 300 nm, corresponding to the π–π* transitions of the aromatic C=C bonds. Transmission electron microscopic (TEM) analysis verified the uniform spherical morphology with a particle size < 2.45 nm. FT-IR analysis confirmed the presence of C=C stretching vibrations. The Box–Behnken (BB) Design was used to reconnoiter the influence of three variables on the response parameter: the F0/F ratio. The best performance could be achieved at a pH of 9, after only 300 s, and a temperature of 50 °C. The developed CTAB-CDs-based nanoprobe showed an ON–OFF behavior when came in contact with cadmium (II) and demonstrated high sensitivity with a limit of detection as low as 0.06 µM (0.007 ppm) over a wide linear range of 2–103 µM. Validation tests confirmed the applicability of the CTAB-CDs-based nanoprobe for detecting cadmium (II) in tap and drinking waters with high accuracy and precision. [ABSTRACT FROM AUTHOR]

For millennia, springs have provided water for drinking, domestic use, balneological treatment, liminality rites as well as tourist attractions. Amidst these uses, anthropogenic activities, especially urbanization and agriculture, continue to impair the functionality of springs. With the looming decadal climate change, freshwater springs could be a sustainable source of clean water for the realisation of Sustainable Development Goal 6. This paper presents the results of the sanitary inspection and assessment of limnochemical characteristics and quality of water samples (n = 64) from four freshwater springs (coded SPR1, SPR2, SPR3, and SPR4) in Kansanga, a flash flood-prone area in the African Great Lakes region of Uganda. Each sample was analysed for 17 parameters (temperature, pH, electrical conductivity, turbidity, fluorides, sulphates, chlorides, nitrates, orthophosphates, total dissolved solids, dissolved oxygen, total alkalinity, potassium, sodium, total, magnesium and calcium hardness) following the standard methods. Water quality index (WQI) was calculated to establish the quality of the water samples based on the physicochemical parameters measured. Based on the sanitary risk assessment results, the springs had medium- to high-risk scores, but most water parameters were within the WHO guidelines for potable water, except for nitrates (in SPR1 and SPR2), hardness levels (in SPR2), and dissolved oxygen (in all the samples). Sampling season and location had significant effects on the limnochemistry of the freshwater springs (p < 0.05). The water quality indices calculated indicated that the water from the springs was of good quality (WQI = 50–57), but there was a reduction in water quality during the wet season. The best water quality was recorded in samples from SPR4, followed by those for SPR3, SPR1, and SPR2. These results provide insights into the contribution of floods and poor sanitation facilities to the deterioration of spring water quality in Kansanga, and the need to leverage additional conservation strategies to support vulnerable communities in the area. Further studies are required to establish the risk posed by trace metals and microbes that may contaminate freshwater in the studied springs, especially following flood events. [ABSTRACT FROM AUTHOR]

Vegetables are an essential component of a balanced diet. The consumption of ready-to-eat foods may lead to the risk of infections and illnesses due to microbial contamination. To mitigate the potential of microbial contamination risks, it is critical to promote safe handling practices among consumers. In this study, our research evaluated the efficacy of different vegetable washing methods, specifically with lettuce, tomato, and cucumber, to establish optimal practices for reducing microbial contamination. This study consisted of two phases. Initially, a survey was distributed to 150 volunteers using snowball sampling to assess everyday vegetable handling and washing methods. The survey's results identified four predominant methods: washing with a 5% vinegar solution for 3 min followed by tap water rinse (37.3% of participants), rinsing with tap water for 1 min (29.3%), washing with a 5% salt solution (vegetable soap) for 3 min followed by a tap water rinse (16.6%), and a 3 min tap water rinse (14%). A minor segment (3.33%) reported not washing their vegetables at all. The survey's findings guided the second phase, which tested the aforementioned washing protocols' effectiveness in reducing Escherichia coli (E. coli) levels on spiked contaminated salad vegetables. The tested vegetables were sterilized using UV light, inoculated with 0.5 McFarland E. coli, and then washed using the four identified methods. After that, E. coli enumeration after washing was performed using 3M™ Petrifilm and the comparison was analyzed via one-way ANOVA. During this study, it was revealed that the cucumbers had the highest E. coli contamination levels in comparison to the lettuce and tomato after washing. Interestingly, by comparing the three washing methods, it was found that washing the vegetables with vinegar proved to be the most effective solution for reducing microbial presence on both lettuce and cucumbers. Notably, the natural smoothness of tomato skin led to no significant differences in contamination levels across washing methods. In summary, vinegar washing effectively reduces microbial contamination from salad vegetables, highlighting the need for informed consumer practices to prevent foodborne outbreaks. This study emphasizes the importance of monitoring contamination sources and using safe washing techniques. [ABSTRACT FROM AUTHOR]

Direct Contact Membrane Distillation (DCMD) uses low heat sources to separate water from urea, which was then used as a plasticizer in regolith-based cement to make it more workable. The work investigated separating potable water and urea from artificial urine using DCMD and then characterizing the products. Water was successfully separated from the artificial urine solution as characterized by density, conductivity, pH, and substance concentrations. The concentrated urine solution was used in regolith-based cement cured under vacuum at temperatures that simulated temperatures that would be expected in construction on the Moon. Workability and other properties were improved by replacing water with concentrated urine solution in the mix. [ABSTRACT FROM AUTHOR]

Microplastics (MPs) contribute to the overall pollution of water sources, affecting not only aquatic ecosystems but also water for human consumption (WHC). Currently, there needs to be a global consensus on safe levels of microplastics in WHC, which will allow regulatory efforts and risk assessments to be carried out. Therefore, this study aims to characterize MP particles in WHC of the Lisbon water supply system (LWSS) and compare two approaches to quantify these particles (length and width of the particles, and the area equivalent diameter (AED) of the particles). The quantification of MP particles was made via micro-FTIR (Fourier Transform Infrared Spectroscopy) on transmission mode after water filtration on 5 µm silicon filters. Thirty-eight WHC samples from the LWSS showed MPs up to 836 MPs/L, with an average value of 196 MPs/L. The most representative polymer was polyethylene (PE, 77.2%). The other eight polymers were also quantified. The length and width of MPs ranged between 84 µm and 41 µm, respectively. The AED of MPs ranged between 24 µm and 405 µm. The MP dimensions of both approaches can differ significantly. [ABSTRACT FROM AUTHOR]

Euphorbia kansui, a toxic Chinese medicine used for more than 2000 years, has the effect of "purging water to promote drinking" and "reducing swelling and dispersing modules". Diterpenes and triterpenes are the main bioactive components of E. kansui. Among them, ingenane-type diterpenes have multiple biological activities as a protein kinase C δ (PKC-δ) activator, which have previously been shown to promote anti-proliferative and pro-apoptotic effects in several human cancer cell lines. However, the activation of PKC subsequently promoted the survival of macrophages. Recently, we found that 13-hydroxyingenol-3-(2,3-dimethylbutanoate)-13-dodecanoate (compound A) from E. kansui showed dual bioactivity, including the inhibition of tumor-cell-line proliferation and regulation of macrophage polarization. This study identifies the possible mechanism of compound A in regulating the polarization state of macrophages, by regulating PKC-δ-extracellular signal regulated kinases (ERK) signaling pathways to exert anti-tumor immunity effects in vitro, which might provide a new treatment method from the perspective of immune cell regulation. [ABSTRACT FROM AUTHOR]

Heavy metal pollution is a critical issue affecting the safety of drinking water sources. However, the impact of human activities on heavy metal risk levels in water-carrying lakes remains unclear. This study aims to explore the risk mechanisms of heavy metals in Luoma Lake, an important water-carrying lake for the South-to-North Water Diversion Project. We explored the spatial and temporal differences in the distribution of heavy metals in Lake Luoma using methods such as the heavy metal pollution index (HPI) and assessed the risk variations using a health assessment model. The results indicated that heavy metal concentrations in water-carrying lakes generally decreased during the dry season, with Mn and Zn levels decreasing by 89.3% and 56.2%, respectively. The comprehensive score of HPI decreased by 13.16% following the retreating polder compared to the control area (Non-retreating polder area). Furthermore, the HPI at the drinking water intake was lower, which is closely associated with the elevated dissolved oxygen (DO) and oxidation–reduction potential (ORP) resulting from water diversion. The annual average health risk across the entire lake was not significant, with higher levels observed in the control area. The annual non-carcinogenic risk levels of Mn, Ni, Cu, Zn, and Pb range from 10−13 to 10−9, which are considered negligible risk levels. Notably, the carcinogenic risk posed by arsenic (As) through the drinking pathway reached 10−5 a−1, exceeding the maximum levels recommended by certain organizations. These findings provide a critical foundation for managing heavy metals in water-carrying drinking water sources. [ABSTRACT FROM AUTHOR]

In recent years, sudden water pollution accidents have frequently occurred and seriously endangered people's drinking water safety. Early warnings for water pollution accidents has become the core work for emergency response and sparked substantial research. However, risk assessments for different water receptors still needs to be solved for the early warning of water pollution accidents. This paper proposes a new early warning assessment method based on water quality models for different receptors, divided into the water source area (including drinking water source area and agricultural water use area) and the non-water source area. The constructed method was applied to the Three Gorges Dam area in China to simulate a phosphorus leakage accident caused by a traffic accident. Based on the EFDC model, the migration evolution process and the distribution change characteristics of phosphorus were simulated. Accordingly, the different risk levels of zones between the incident site and the downstream drinking water source area were obtained. The application results show that the risk warning system for sudden water pollution accidents based on the water quality model proposed in this paper can be applied to provide scientific support for the emergency response of sudden water pollution accidents. [ABSTRACT FROM AUTHOR]

A novel comprehensive resilience assessment framework for drinking water systems is proposed integrating different resilience perspectives (i.e., robustness, autonomy, flexibility, reliability, preparedness and recovery), oriented by objectives, criteria and metrics, applicable at the tactical level. The resilience assessment framework is applied to a Portuguese real water distribution network, enabling the evaluation of the system's resilience. The infrastructure dimension is the main contributor to the low resilience results, particularly in terms of infrastructural robustness, as the infrastructure has exceeded the average service life and has low rehabilitation rates. In terms of autonomy, the system highly depends on external water and energy sources. Regarding the service dimension, most of the drinking water available is used for non-potable uses (e.g., irrigation), without alternative sources. The detailed diagnosis identified network area R6 as the priority area. Assets rehabilitation, increasing storage capacity, finding alternative water and energy sources, and minimizing non-potable uses are relevant improvement measures that promote the reinforcement of the system's resilience. The resilience assessment framework is a very useful tool for the daily and tactical management of drinking water systems. [ABSTRACT FROM AUTHOR]

Extreme flood events present a significant challenge for operators and managers of large drinking water reservoirs. Detailed flood response analysis can predict the hydrology response of a reservoir to changing climate conditions and can aid in managing the reservoir in anticipation of extreme events. Herein, the Soil and Water Assessment Tool (SWAT), a watershed model, was used in conjunction with a reservoir management model, the Operational Analysis and Simulation of Integrated Systems (OASIS) model, to evaluate extreme flood events across a set of initial reservoir storage capacities across various CMIP6 climate scenarios. The SWAT model was calibrated and validated with PRISM climate data in conjunction with land and soil cover data and multi-site gauged stream discharges. The validated model demonstrated satisfactory performance (NSE = 0.55 and R2 = 0.56) for total reservoir inflow. The resulting inflow values from SWAT were utilized to set up a calibrated/validated OASIS model (NSE = 0.55 and R2 = 0.68). OASIS was then used to assess alternative operating rules for the reservoir under varying climate scenarios (RCP4.5 and RCP8.5) and extreme events (synthetic hurricanes). Focusing on a major reservoir in the Northeastern United States, the analysis of the reservoir response was based on (1) reservoir volume–elevation curve, (2) daily reservoir inflow, (3) daily precipitation, (4) spillway flow, and (5) reservoir evaporation. Projected future scenarios indicate a >20% increase in precipitation in April compared to historical records, coupled with likely reduced runoff from November to March. With extreme conditions most likely in the month of April, RCP4.5 and RCP8.5 projections suggest that most scenarios result in a 10–15% increase in the mean of 3D30Y runoff volumes, and a 150% increase under the most extreme conditions. For 7D30Y runoff volumes in April, the RCP4.5 and RCP8.5 analyses reveal an increased likelihood of the reservoir elevation reaching overspill flow levels during the latter half of the simulation period (2020 to 2080). Our findings indicate that simulations with SWAT coupled with OASIS can assist reservoir managers in regulating water levels in anticipation of extreme precipitation events. [ABSTRACT FROM AUTHOR]

This study describes the implementation of Internet of Things (IoT) sensors in flow meters installed in drinking water systems and in fog catchers built in low-income, high-altitude communities in the Andes region of Ecuador, taking studies at the University de las Fuerzas Armadas ESPE as our reference. The influence and management of these intelligent sensors are analyzed, as well as a basic review of the materials and methods used in their implementation. The importance of validating the accuracy and reliability of IoT sensors compared to professional devices is highlighted, especially in mountain areas with difficult access. Additionally, the cost–benefit of using IoT sensors in fog catchers and drinking water distribution networks is mentioned, which depends on several factors such as the scale of the project, the objectives to be achieved concerning monitoring, and the available resources. Finally, it is highlighted that using Internet of Things (IoT) sensors in construction and water collection systems has proven beneficial in detecting possible effects on its operation and determining consumption and supply flows for a given population. [ABSTRACT FROM AUTHOR]

Growing concerns over public health and environmental safety have intensified the focus on minimizing harmful disinfection byproducts (DBPs) in water treatment. Traditional methods like chlorination, while effective against pathogens, often lead to the formation of DBPs, which pose significant risks. This paper explores alternative strategies to reducing DBP formation while ensuring effective disinfection. The methodology involved a bibliographic study conducted through the Scopus platform, using appropriate keywords. The initial search yielded 9576 articles from the period 2020 to 2024. The key approaches identified include advanced oxidation processes (AOPs) such as UV/H2O2 and ozone, which mineralize natural organic matter (NOM) and minimize chemical use and sludge production; membrane-based filtration systems, like reverse osmosis, effectively removing contaminants without chemical disinfectants, reducing DBP risks. Furthermore, conventional processes, such as coagulation and filtration, serve as crucial pretreatment steps to lower NOM levels before disinfection. Additionally, optimizing chlorine dosing, using non-chlorine disinfectants, and employing post-disinfection methods like adsorption and biological filtration further mitigate DBP formation. Finally, the integration of artificial intelligence in process optimization is emerging as a promising tool for enhancing treatment efficiency and safety. This research contributes to the development of safer, more sustainable water treatment solutions, addressing regulatory demands and public health objectives. [ABSTRACT FROM AUTHOR]

The aim of this research was to synthesize alginate beads. The beads were modified with a mixture of three different species of algae. Both synthesized beads were evaluated for the efficiency of Cd(II) removal from aqueous solutions as one of the currently most sustainable metal removal methods. The focus was on the characterization of synthesized beads and their stability. The characterization was performed using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The specific surface area was determined. Cd(II) ion standard solutions were brought into contact with unmodified and modified beads. The experimental results showed that the most influential factors on biosorption are pH value and temperature. The maximum biosorption of Cd(II) ions was achieved at 181.0 mg/g. Kinetic and thermodynamic studies were carried out. The data obtained followed pseudo-second-order kinetics. [ABSTRACT FROM AUTHOR]

Groundwater and surface water have been the primary sources of our public water supply around the world. However, rapid population and economic growth, as well as global climate change, are posing major threats to the quality and quantity of these water resources. Treated wastewater (reclaimed water) and stormwater are becoming more important water resources. Use/reuse of these unconventional water resources can enable a truly sustainable, closed-loop, circular water system. However, these two sources are not usually mixed with each other. In this study, we propose the use of combined excess urban stormwater and reclaimed water as a source of potable water supply. One of the most pronounced benefits of this proposed scheme is the possible elimination of costly and energy-intensive processes like reverse osmosis. Reclaimed water tends to have high concentrations of dissolved solids (>500 mg/L) and nitrate-N (>10 mg/L), which can be lowered by blending with stormwater or rainwater. Despite technical and engineering challenges, this approach can benefit various communities—small, medium, large, upstream, downstream, urban, and rural—in diverse climates. Our study suggests that this new holistic approach is feasible, enabling the combined water to be directly used as a sustainable drinking water source. [ABSTRACT FROM AUTHOR]

Nitrate (NO3−) is a vulnerable natural contaminant that can be found in groundwater. The estimated nitrate concentrations for four categories of wells in the northeastern arid regions of Saudi Arabia—commercial treated water stations for drinking, commercial stations of untreated water for domestic uses, private wells of residences for households, and private wells for agricultural uses—were found to be in the 16–380 mg/L range. Drinking water from all commercial treated water stations has lower nitrate levels, based on the WHO standard of 50 mg/L. In contrast, almost 33% of commercial stations with untreated water (used only for domestic purposes) in the studied areas had higher nitrate levels that were unsuitable for drinking. Approximately half of the private wells of residences and wells for agricultural uses had very high nitrate levels. They can be considered unsuitable for drinking due to excessive levels of nitrates but can be used for domestic and agricultural purposes. Thus, adopting specific strategies to reduce nitrate levels in public wells in the studied areas is crucial. The data obtained in the present study are essential for equipping decision-makers with valuable insights, allowing them to enact appropriate measures, as needed, and uphold community health in the studied regions. [ABSTRACT FROM AUTHOR]

Invertebrates, including Asellidae, are part of the natural ecosystem of the drinking water distribution system (DWDS) and are known to cause a nuisance to consumers. In addition, recently, the potential role of the species Asellus aquaticus (L. 1758) in the regrowth of Aeromonas bacteria was published. Aeromonas is included in the Dutch drinking water guidelines as a process parameter, and the guideline values are regularly exceeded. Although neither A. aquaticus nor Aeromonas is associated with health risks, the Evides drinking water utility shows a strong interest in the possible reasons for these exceedances and possible control measures. In surface waters, Asellidae feed mainly on decaying leaves that are abundantly present. These food sources are not present in the DWDS. Therefore, we determined suitable food sources for A. aquaticus in the DWDS. Laboratory experiments show that A. aquaticus individuals survive on biofilm on pipe wall material and loose deposits (sediments) collected from DWDS. Growth and survival rates on these loose deposits were even higher than on the positive control (decaying leaves). As the basis of these loose deposits is inorganic (iron deposits, sand, and pipe particles), the organic matter (living and decaying bacteria, protozoans, fungi, and invertebrates) must be their substrate. These experiments validate hypotheses that Asellidae can grow and survive on organic matter in deposits in DWDS. [ABSTRACT FROM AUTHOR]

The global microplastic pollution issue, as a result of the indispensable usage of microplastics in building materials, packaged food, medical products and consumer goods, poses significant health problems for the population. These small particles can penetrate intact cell barriers in the intestines and alveoli, thereby entering the bloodstream. The aim of this pilot study was to investigate the effects of reduced plastic consumption on blood pressure. Eight adult and healthy participants abstained from consuming commercially produced bottled beverages and restricted their primary fluid intake to tap water. Blood pressure was measured on both sides before, after 14 days and after 28 to 30 days of this partial plastic diet. Women exhibit a significant change in systolic blood pressure on the right arm after 2 and 4 weeks, while the left arm demonstrates no significant changes in blood pressure. On the contrary, in men, systolic blood pressure values on both arms show no significant alterations, attributable to the high variability across the three participants. Moreover, no significant differences in systolic blood pressure were observed when analysing the entire cohort. Significant findings are evident only at the two-week mark for diastolic blood pressure for all participants in both arms. When considering diastolic blood pressure separately for women and men, men again show no significant changes in blood pressure on either arm. However, women exhibit a significant decrease in diastolic blood pressure on the left arm after 2 weeks and a statistically significant decline in diastolic blood pressure on the right arm after both 2 and 4 weeks. The results of the study suggest, for the first time, that a reduction in plastic use could potentially lower blood pressure, probably due to the reduced volume of plastic particles in the bloodstream. To confirm this hypothesis, a larger sample of male and female participants must be examined, ideally with the monitoring of plastic concentration in the blood. [ABSTRACT FROM AUTHOR]

This study explores the feasibility of producing electrolyzed water (EW) for post-harvest treatment of fruits and vegetables as a new substitute for current chemical products. A prototype generator using tap water and NaCl solution was tested for EW's sanitization efficiency, energy, and economic costs. In vitro tests on Penicillium e., Aspergillus n., Botrytis c., and Alternaria a. assessed EW pH, chlorine concentration, electro-oxidative potential, pathogen contact time, and energy consumption. Optimal results were achieved with a pH of 4.6, electro-oxidative potential of 188 mV, active chlorine concentration of 3.4 mg/L, and a contact time of 1–2 min. The prototype produced 10.0 L of EW in 1 h, consuming 0.11 kWh of electricity. Real-scale energy consumption was 545 kWh/m3 EW, costing 12.51 euro/m3. The study concludes that optimizing EW production can reduce energy consumption, making it a viable alternative for industrial sanitization of fruits and vegetables. [ABSTRACT FROM AUTHOR]

Population growth, coupled with industrial and agricultural development, has resulted in increased demand for freshwater supply. For countries with scarce water resources, desalination constitutes the only viable solution to this problem. Reverse osmosis (RO) technology has become widely used as the membrane materials have been upgraded and the costs have been reduced. Nowadays, RO is the foremost technology for desalting different types of water such as seawater, brackish, and tap water. However, its design is critical since many parameters are involved in obtaining a good design. The high use of RO encourages the establishment of a procedure that facilitates the design process and helps in obtaining an optimum-performance RO desalination system. This paper presents a procedure divided into three parts: (1) classifying RO parameters; (2) choosing the parameters in a certain order and doing the calculation process through 12 steps; and (3) then inserting the selected parameters and the obtained values on RO System Analysis (ROSA) software. These points are then summarized by creating an algorithmic chart to follow during the design phase of the RO system using ROSA. An example on the proposed list is then taken to validate the procedure, and a comparison is conducted on choosing different values for the parameters. The results of this comparative study show that choosing different parameters affects the RO system productivity. Additionally, every design has a specific optimum set of parameters, which depends upon the design constraints set by the user. [ABSTRACT FROM AUTHOR]

Arundo donax (family: Poaceae) is a perennial rhizomatous plant with considerable economic and ecological benefits. The plant is characterized by its fast growth, high yield, wide adaptability, and strong disease resistance. However, widespread cultivation is difficult because A. donax can only be asexually propagated. This study tested the efficacy of an aqua-based culture method with short duration and high rooting rate. The aqua-based culture protocol involved treating cane fragments with the commercial broad-spectrum rooting powder ABT-1 (No. 1 of the ABT series) at 100 mg/L for 24 h, followed by culturing in tap water for 19 days. We found that plant materials sampled from September to February of the following year were more suitable for aqua-based culturing than materials sampled from other months. Nodes on different parts of the cane did not vary significantly in the ability to form seedlings. The rooting percentage reached >95% after only 20 days, while the sprouting percentage was >93%. The seedlings obtained were strong, with high survival rates during cultivation and acclimation. No strict requirements for the growing media were necessary to achieve these results. In conclusion, this study provides an empirical foundation for a technique that could facilitate the factory production of high-quality A. donax seedlings. [ABSTRACT FROM AUTHOR]

Acute liver injury is an increasing global health problem. It is a widespread side effect of cisplatin treatment in the clinic and can lead to liver failure if not treated promptly. Previous studies have revealed that green tea can protect some organs from treatments. However, the potential of white tea to prevent the negative effects of acute liver injury has not been addressed so far. The purpose of this study was to investigate the reduction in cisplatin-induced liver injury in rats receiving white tea. Female Sprague Dawley rats with similar weight were selected in this study. Twenty-four rats were divided into three groups of eight animals each and ad libitum nutrition was provided. The control and cisplatin groups were given tap water only, while the white tea + cisplatin group received white tea at a 0.5% weight/volume concentration for four weeks. At the end of the fourth week, the white tea + cisplatin group and the cisplatin group received a single dose of cisplatin (7 mg/kg) via the intraperitoneal route. Five days after that procedure, the rats were anesthetized. Liver tissues and blood samples were collected, which were used for biochemical and histopathological analyses. According to biochemical results, liver tissue MDA and GSH, serum ALT, and AST levels significantly increased in the cisplatin group compared to the control group. Compared with the cisplatin group, although MDA, AST, ALT, and GSH levels were lower in the white tea + cisplatin group, only GSH levels were statistically different. The examination of histopathological and immunohistochemical findings revealed apoptotic cells, vascular congestion, and sinusoidal dilatation in the cisplatin group compared to the control group. This adverse event decreased in the white tea + cisplatin group compared to the cisplatin group. In conclusion, white tea exhibits an ameliorating effect on cisplatin-induced liver injury. [ABSTRACT FROM AUTHOR]

Utilizing the full potential of reproducible biomass resources is crucial for the sustainable development of humanity. In this study, biochar (MPC) was prepared through the microwave-assisted pyrolysis of sugarcane bagasse. Subsequently, Co nanoparticles were introduced by microwave-assisted hydrothermal treatment to form a highly dispersive Co/MPC material. Characterization results indicated that Co nanoparticles were wrapped by thin carbon layers and uniformly dispersed on a carbon-based skeleton via a microwave-assisted hydrothermal synthesis approach, providing high-activity space. Thus, the prepared material was limited to glassy carbon; on the electrode surface, a cobalt-based sensing platform (Co/MPC/GCE) was built. On the basis of this constructed sensing platform, a linear equation was fitted by the concentration change of current signal I and H2O2. The linear range was 0.55–100.05 mM; the detection limit was 1.38 μM (S/N = 3); and the sensitivity was 103.45 μA cm−2 mM−1. In addition, the effect this sensor had on H2O2 detection of actual water samples was conducted by using a standard addition recovery method; results disclosed that the recovery rate and RSD of H2O2 in tap water samples were 94.0–97.6% and 4.1–6.5%, respectively. [ABSTRACT FROM AUTHOR]

Kefir has been associated with beneficial effects on its host's health. The previous works examining the impact of kefir on the immune system focused on milk kefir or the exopolysaccharides and bacterial strains derived from it, while water kefir has not been evaluated. Furthermore, studies have focused on kefir's ability to modulate immune system hemostasis and exert anti-inflammatory effects, while its specific action on antiviral immunity has not been investigated. Thus, the aim of this work was to examine the potential immunomodulatory effects of water kefir on the intestinal innate antiviral immunity mediated by Toll-like receptor-3 (TLR3). Adult BALB/c mice fed water kefir ad libitum, diluted 1:5, 1:10, or 1:20 in the drinking water, for 6 consecutive days. On day 7, the treated groups and the untreated control mice received an intraperitoneal injection of the TLR3 agonist poly(I:C). Two days after the TLR3 activation, the intestinal damage and the innate immune response were studied. The intraperitoneal administration of poly(I:C) induced inflammatory-mediated intestinal tissue damage, characterized by the upregulation of interferons (IFNs), pro-inflammatory mediators (TNF-α, IL-15, IL-6), and factors involved in epithelial destruction (RAE-1 and NKG2D). The histological analysis of small intestinal samples showed that mice receiving water kefir 1:5 exhibited reduced edema and a lower inflammatory cell infiltration. Kefir-treated mice had significantly lower levels of serum LDH, AST, and ALT as well as intestinal TNF-α, IL-15, IL-6, RAE-1, and NKG2D. This group also showed higher concentrations of intestinal IFN-β, IFN-γ, and IL-10. The treatment with 1:10 of water kefir reduced intestinal damage and modulated cytokines but its effect was significantly lower than the 1:5 treatment, while the water kefir 1:20 did not modify the parameters evaluated compared to control mice. The results indicate that water kefir exerts its immunomodulatory effects in a dose-dependent manner. The in vivo studies allow us to speculate that water kefir can induce two beneficial effects on the intestinal TLR3-mediated immune response: the enhancement of antiviral defenses and the protection against the inflammatory-mediated tissue damage. These protective effects of water kefir require further exploration to understand how water kefir, or its specific molecules/strains, can influence the immune response and to determine the extent of its protection against a real viral challenge. [ABSTRACT FROM AUTHOR]

Identified as a potential reference pathogen by the WHO Guidelines for Drinking-Water Quality, Rotavirus (RV) is among the main enteric viruses that cause waterborne diseases. The aim of this study was to identify and correlate the presence of RV in collective and individual water sources of rural communities in the state of Goiás, within the seasons in which the collections were made (rainy and dry seasons). For this, 86 water samples in the dry period and 160 samples in the rainy period were collected. Concentration of water samples, extraction of viral genetic material and molecular tests were performed. When analyzing the presence of RV in the samples, taking into consideration the period studied, RV was found to be more prevalent in the dry season (54.7%) than in the rainy season (20%), showing a strong statistical association with the dry season (p-value < 0.001). The presence of pathogenic microorganisms in water is a public risk issue, enabling the emergence of outbreaks, endemics and epidemics. In the present research, there was an association between the presence of Rotavirus and the dry period of the year when compared to the rainy period. [ABSTRACT FROM AUTHOR]

In this study, a new and simple glassy carbon electrode modified with carbon nanohorns (SWCNH/GCE) was used for the determination of Cr(VI) in aqueous matrices via adsorptive cathodic stripping voltammetry (AdCSV). The modified electrode was characterized via field emission scanning electron microscopy and cyclic voltammetry, which revealed a homogeneous distribution of spherical agglomerates of SWCNH on the electrode surface. The modification increased the electrochemically active area from 0.10 cm2 ± 0.01 (GCE) to 0.16 cm2 ± 0.01 (SWCNH/GCE). The optimized analytical conditions were as follows: a supporting electrolyte (0.15 mol L−1 HCl), an accumulation potential of 0.8 V versus Ag/AgCl, and an accumulation time of 240 s. Validation of the analytical methodology was performed, obtaining a linear range between 20 and 100 µg L−1, a limit of detection of 3.5 µg L−1, and a limit of quantification of 11.6 µg L−1 with good accuracy and precision. The method was applied to the analysis of spiked tap water samples, and the results were compared using a flame atomic absorption spectrophotometer (FAAS) with no significant statistical differences. [ABSTRACT FROM AUTHOR]

Purslane has been suggested as an alternative crop suitable for human consumption due to its high content of minerals, omega-3 fatty acids, and several health-beneficial compounds. In this study, we aimed to evaluate the effect of salinity stress (tap water (control), 2000, 4000, 6000, 8000, and 10,000 mg L−1), biostimulant application (putrescine and salicylic acid at 200 mg L−1), and the combination of the tested factors (i.e., salinity × biostimulant application) on the growth and chemical composition of purslane plants (Portulaca oleracea L.) over two growing seasons (2022 and 2023). Irrigation with tap water and putrescine application resulted in the highest plant height, weight of aboveground and underground parts, and number of shoots per plant. In contrast, the lowest values of growing parameters were recorded under severe saline stress (10,000 mg L−1), especially for the plants that were not treated with biostimulants. The same trends were observed for macronutrients (N, P, K), total carbohydrates, total chlorophylls, and vitamin C content in leaves. Moreover, nitrate and proline content was higher in plants grown under salinity stress, especially under severe stress (8000–10,000 mg L−1) without biostimulant application. In general, the application of biostimulants mitigated the negative impact of salinity on plant growth and leaf chemical composition, while the effect of putrescine on the tested parameters was more beneficial than that of salicylic acid. In conclusion, this study provides useful information regarding the use of putrescine and salicylic acid as biostimulatory agents with the aim of increasing purslane growth under salinity conditions. [ABSTRACT FROM AUTHOR]