1. Genomic Sequence Analysis and Organization of BmKαTx11 and BmKαTx15 from Buthus martensii Karsch: Molecular Evolution of α-toxin genes
- Author
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Xin Mao, Wen Lan Wu, Wenxin Li, Da He Jiang, Hui Liu, Xiu Ling Xu, Yong Gang Sha, Zhi Jian Cao, Feng Luo, and Ji Qun Sheng
- Subjects
DNA, Complementary ,Sequence analysis ,Molecular Sequence Data ,Scorpion Venoms ,Locus (genetics) ,Biology ,Biochemistry ,Evolution, Molecular ,Scorpions ,Gene Duplication ,Sequence Homology, Nucleic Acid ,Complementary DNA ,Gene duplication ,Animals ,Gene family ,Amino Acid Sequence ,Molecular Biology ,Gene ,Southern blot ,Genetics ,Genome ,Base Sequence ,General Medicine ,Introns ,Blotting, Southern ,genomic DNA ,Mutation ,Gene Deletion - Abstract
Based on the reported cDNA sequences of BmKalphaTxs , the genes encoding toxin BmKalphaTx11 and BmKalphaTx15 were amplified by PCR from the Chinese scorpion Buthus martensii Karsch genomic DNA employing synthetic oligonucleotides. Sequences analysis of nucleotide showed that an intron about 500 bp length interrupts signal peptide coding regions of BmKalphaTx11 and BmKalphaTx15. Using cDNA sequence of BmKalphaTx11 as probe, southern hybridization of BmK genome total DNA was performed. The result indicates that BmKalphaTx11 is multicopy genes or belongs to multiple gene family with high homology genes. The similarity of BmKalpha-toxin gene sequences and southern hybridization revealed the evolution trace of BmKalpha-toxins: BmKalpha-toxin genes evolve from a common progenitor, and the genes diversity is associated with a process of locus duplication and gene divergence.
- Published
- 2005