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Genomic Sequence Analysis and Organization of BmKαTx11 and BmKαTx15 from Buthus martensii Karsch: Molecular Evolution of α-toxin genes
- Source :
- BMB Reports. 38:386-390
- Publication Year :
- 2005
- Publisher :
- Korean Society for Biochemistry and Molecular Biology - BMB Reports, 2005.
-
Abstract
- Based on the reported cDNA sequences of BmKalphaTxs , the genes encoding toxin BmKalphaTx11 and BmKalphaTx15 were amplified by PCR from the Chinese scorpion Buthus martensii Karsch genomic DNA employing synthetic oligonucleotides. Sequences analysis of nucleotide showed that an intron about 500 bp length interrupts signal peptide coding regions of BmKalphaTx11 and BmKalphaTx15. Using cDNA sequence of BmKalphaTx11 as probe, southern hybridization of BmK genome total DNA was performed. The result indicates that BmKalphaTx11 is multicopy genes or belongs to multiple gene family with high homology genes. The similarity of BmKalpha-toxin gene sequences and southern hybridization revealed the evolution trace of BmKalpha-toxins: BmKalpha-toxin genes evolve from a common progenitor, and the genes diversity is associated with a process of locus duplication and gene divergence.
- Subjects :
- DNA, Complementary
Sequence analysis
Molecular Sequence Data
Scorpion Venoms
Locus (genetics)
Biology
Biochemistry
Evolution, Molecular
Scorpions
Gene Duplication
Sequence Homology, Nucleic Acid
Complementary DNA
Gene duplication
Animals
Gene family
Amino Acid Sequence
Molecular Biology
Gene
Southern blot
Genetics
Genome
Base Sequence
General Medicine
Introns
Blotting, Southern
genomic DNA
Mutation
Gene Deletion
Subjects
Details
- ISSN :
- 19766696
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....cf01fc7cda39d39b9fa44ef14ed1d8c5