1. A new method for measuring osteoclast formation by electrical impedance.
- Author
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Emori H, Iwai S, Ryu K, Amano H, Sambe T, Kobayashi T, Oguchi T, Ohura K, and Oguchi K
- Subjects
- Bone Resorption pathology, Bone Resorption prevention & control, Catechin analogs & derivatives, Catechin pharmacology, Cell Differentiation, Cells, Cultured, Humans, Macrophage Colony-Stimulating Factor, RANK Ligand, Computer Systems, Cytological Techniques methods, Electric Impedance, Osteoclasts cytology
- Abstract
Osteoclasts are important target cells for osteoporosis treatment. Recently, a real-time cell analysis (RTCA) system was developed to observe cell morphology and adhesion; however, the use of RTCA to study osteoclastogenesis has not been reported. Here, we investigated whether osteoclast formation could be monitored in real-time using RTCA. The cell index determined via electrical impedance using RTCA, and the number of osteoclasts exhibited a significant positive correlation. RTCA was useful for determining the effect of (-)-epigallocatechin-3-gallate on the inhibition of bone resorption. We established a new method of measuring osteoclast formation in real-time using RTCA., (Copyright © 2015. Production and hosting by Elsevier B.V.)
- Published
- 2015
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