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Start Over Author "Yin TC" Publisher hindawi pub. corp
4 results on '"Yin TC"'

1. Transforaminal Interbody Impaction of Bone Graft to Treat Collapsed Nonhealed Vertebral Fractures with Endplate Destruction: A Report of Two Cases.

Author

Wegner AM, Chou YH, Chang HK, and Yin TC

Abstract

Background: A collapsed nonhealed vertebral fracture with endplate destruction is a challenging injury to address, as there is no single definitive treatment. We present two cases using an innovative transforaminal grafting technique to treat these patients. Case Presentation . Case 1: a 72-year-old woman had nonunion of an L1 compression fracture with destruction of both endplates. T12/L1 and L1/L2 transforaminal debridement and impaction of bone graft were performed followed by posterior instrumentation. At three years follow-up, the fusion mass between T12/L1 and L1/L2 was solid and the patient had minimal pain. Case 2: a 62-year-old woman had nonunion of an L1 burst fracture with destruction of the lower endplate. Hemilaminectomy and transforaminal interbody impaction of bone graft was performed. At three years follow-up, the patient had no back pain and a solid fusion. In both cases, local kyphosis was corrected and fusion obtained., Conclusions: Collapsed nonhealed vertebral body fractures combined with endplate destruction can be successfully treated with a one-step posterior surgery consisting of transforaminal debridement and impaction of bone graft in combination with posterior pedicle instrumentation., Competing Interests: The authors declare that they have no competing interests., (Copyright © 2020 Adam M. Wegner et al.)

Published
2020
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2. Shock Wave Therapy Enhances Mitochondrial Delivery into Target Cells and Protects against Acute Respiratory Distress Syndrome.

Author

Lin KC, Wallace CG, Yin TC, Sung PH, Chen KH, Lu HI, Chai HT, Chen CH, Chen YL, Li YC, Shao PL, Lee MS, Sheu JJ, and Yip HK

Subjects
Animals, Flow Cytometry, Intercellular Adhesion Molecule-1 metabolism, Interleukin-1beta metabolism, Male, Matrix Metalloproteinase 9 metabolism, NF-kappa B metabolism, Oxygen Consumption physiology, Rats, Tumor Necrosis Factor-alpha metabolism, Epithelial Cells metabolism, Extracorporeal Shockwave Therapy methods, Mitochondria metabolism, Oxidative Stress physiology, Respiratory Distress Syndrome metabolism, Respiratory Distress Syndrome therapy
Abstract

This study tested the hypothesis that shock wave therapy (SW) enhances mitochondrial uptake into the lung epithelial and parenchymal cells to attenuate lung injury from acute respiratory distress syndrome (ARDS). ARDS was induced in rats through continuous inhalation of 100% oxygen for 48 h, while SW entailed application 0.15 mJ/mm 2 for 200 impulses at 6 Hz per left/right lung field. In vitro and ex vivo studies showed that SW enhances mitochondrial uptake into lung epithelial and parenchyma cells (all p < 0.001). Flow cytometry demonstrated that albumin levels and numbers of inflammatory cells (Ly6G+/CD14+/CD68+/CD11 b/c +) in bronchoalveolar lavage fluid were the highest in untreated ARDS, were progressively reduced across SW, Mito, and SW + Mito (all p < 0.0001), and were the lowest in sham controls. The same profile was also seen for fibrosis/collagen deposition, levels of biomarkers of oxidative stress (NOX-1/NOX-2/oxidized protein), inflammation (MMP-9/TNF- α /NF- κ B/IL-1 β /ICAM-1), apoptosis (cleaved caspase 3/PARP), fibrosis (Smad3/TGF- β ), mitochondrial damage (cytosolic cytochrome c) (all p < 0.0001), and DNA damage ( γ -H2AX+), and numbers of parenchymal inflammatory cells (CD11+/CD14+/CD40L+/F4/80+) ( p < 0.0001). These results suggest that SW-assisted Mito therapy effectively protects the lung parenchyma from ARDS-induced injury.

Published
2018
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3. Extracorporeal Shock Wave-Supported Adipose-Derived Fresh Stromal Vascular Fraction Preserved Left Ventricular (LV) Function and Inhibited LV Remodeling in Acute Myocardial Infarction in Rat.

Author

Sung PH, Yin TC, Wallace CG, Chen KH, Shao PL, Lee FY, Sun CK, Sheu JJ, Chen YL, Omran MM, Chung SY, Wang CJ, Lee MS, and Yip HK

Subjects
Animals, Apoptosis drug effects, Biomarkers metabolism, Cell Line, Cell Nucleus drug effects, Cell Nucleus metabolism, Coculture Techniques, Collagen metabolism, DNA Damage, Echocardiography, Endothelial Progenitor Cells drug effects, Endothelial Progenitor Cells metabolism, Male, Mesenchymal Stem Cells drug effects, Mesenchymal Stem Cells metabolism, Mitochondrial Dynamics drug effects, Myocardial Infarction diagnostic imaging, Myocardial Infarction pathology, Oxidative Stress drug effects, Rats, Sprague-Dawley, Stromal Cells drug effects, Stromal Cells metabolism, Time Factors, Tumor Suppressor p53-Binding Protein 1 metabolism, Ventricular Function, Left drug effects, Ventricular Remodeling drug effects, Vitamin K 3 pharmacology, X-ray Repair Cross Complementing Protein 1 metabolism, Adipose Tissue metabolism, Extracorporeal Shockwave Therapy, Myocardial Infarction physiopathology, Ventricular Function, Left physiology, Ventricular Remodeling physiology
Abstract

This study tested the hypothesis that extracorporeal shock wave- (ECSW-) assisted adipose-derived stromal vascular fraction (SVF) therapy could preserve left ventricular ejection fraction (LVEF) and inhibit LV remodeling in a rat after acute myocardial infarction (AMI). Adult male SD rats were categorized into group 1 (sham control), group 2 (AMI induced by left coronary artery ligation), group 3 [AMI + ECSW (280 impulses at 0.1 mJ/mm 2 , applied to the chest wall at 3 h, days 3 and 7 after AMI), group 4 [AMI + SVF (1.2 × 10 6 ) implanted into the infarct area at 3 h after AMI], and group 5 (AMI + ECSW-SVF). In vitro, SVF protected H9C2 cells against menadione-induced mitochondrial damage and increased fluorescent intensity of mitochondria in nuclei ( p < 0.01). By day 42 after AMI, LVEF was highest in group 1, lowest in group 2, significantly higher in group 5 than in groups 3 and 4, and similar between the latter two groups (all p < 0.0001). LV remodeling and infarcted, fibrotic, and collagen deposition areas as well as apoptotic nuclei exhibited an opposite pattern to LVEF among the groups (all p < 0.0001). Protein expressions of CD31/vWF/eNOS/PGC-1 α / α -MHC/mitochondrial cytochrome C exhibited an identical pattern, whilst protein expressions of MMP-9/TNF- α /IL-1 β /NF- κ B/caspase-3/PARP/Samd3/TGF- β /NOX-1/NOX-2/oxidized protein/ β -MHC/BNP exhibited an opposite pattern to LVEF among five groups (all p < 0.0001). Cellular expressions of CXCR4/SDF-1 α /Sca-1/c-Kit significantly and progressively increased from groups 1 to 5 (all p < 0.0001). Cellular expression of γ -H2AX/CD68 displayed an opposite pattern to LVEF among the five groups (all p < 0.0001). In conclusion, ECSW-SVF therapy effectively preserved LVEF and inhibited LV remodeling in rat AMI.

Published
2018
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4. Combined Therapy with Extracorporeal Shock Wave and Adipose-Derived Mesenchymal Stem Cells Remarkably Improved Acute Ischemia-Reperfusion Injury of Quadriceps Muscle.

Author

Yin TC, Wu RW, Sheu JJ, Sung PH, Chen KH, Chiang JY, Hsueh SK, Chung WJ, Lin PY, Hsu SL, Chen CC, Chen CY, Shao PL, and Yip HK

Subjects
Adiposity, Animals, Humans, Quadriceps Muscle pathology, Rats, Rats, Sprague-Dawley, Extracorporeal Shockwave Therapy methods, Mesenchymal Stem Cells metabolism, Quadriceps Muscle metabolism, Reperfusion Injury therapy
Abstract

Extracorporeal shock wave (ECSW) and adipose-derived mesenchymal stem cells (ADMSCs) have been recognized to have capacities of anti-inflammation and angiogenesis. We tested the hypothesis that ECSW and ADMSC therapy could attenuate ischemia-reperfusion- (IR-) induced thigh injury (femoral artery tightened for 6 h then the tightness was relieved) in rats. Adult male SD rats ( n = 30) were divided into group 1 (sham-control), group 2 (IR), group 3 (IR + ECSW/120 impulses at 0.12 mJ/mm 2 given at 3 h/24 h/72 h after IR), group 4 (allogenic ADMSC/1.2 × 10 6 cell intramuscular and 1.2 × 10 6 cell intravenous injections 3 h after IR procedure), and group 5 (ECSW + ADMSC). At day 7 after the IR procedure, the left quadriceps muscle was harvested for studies. At 18 h after the IR procedure, serum myoglobin/creatine phosphokinase (CPK) levels were highest in group 2, lowest in group 1, and with intermediate values significantly progressively reduced in groups 3 to 5 (all p < 0.0001). By day 5 after IR, the mechanical paw-withdrawal threshold displayed an opposite pattern of CPK (all p < 0.0001). The protein expressions of inflammatory, oxidative-stress, apoptotic, fibrotic, DNA-damaged, and mitochondrial-damaged biomarkers and cellular expressions of inflammatory and DNA-damaged biomarkers exhibited an identical pattern of CPK among the five groups (all p < 0.0001). The microscopic findings of endothelial-cell biomarkers and number of arterioles expressed an opposite pattern of CPK, and the angiogenesis marker was significantly progressively increased from groups 1 to 5, whereas the histopathology showed that muscle-damaged/fibrosis/collagen-deposition areas exhibited an identical pattern of CPK among the five groups (all p < 0.0001). In conclusion, ECSW-ADMSC therapy is superior to either one applied individually for protecting against IR-induced thigh injury.

Published
2018
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