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Extracorporeal Shock Wave-Supported Adipose-Derived Fresh Stromal Vascular Fraction Preserved Left Ventricular (LV) Function and Inhibited LV Remodeling in Acute Myocardial Infarction in Rat.
- Source :
-
Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2018 Oct 17; Vol. 2018, pp. 7518920. Date of Electronic Publication: 2018 Oct 17 (Print Publication: 2018). - Publication Year :
- 2018
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Abstract
- This study tested the hypothesis that extracorporeal shock wave- (ECSW-) assisted adipose-derived stromal vascular fraction (SVF) therapy could preserve left ventricular ejection fraction (LVEF) and inhibit LV remodeling in a rat after acute myocardial infarction (AMI). Adult male SD rats were categorized into group 1 (sham control), group 2 (AMI induced by left coronary artery ligation), group 3 [AMI + ECSW (280 impulses at 0.1 mJ/mm <superscript>2</superscript> , applied to the chest wall at 3 h, days 3 and 7 after AMI), group 4 [AMI + SVF (1.2 × 10 <superscript>6</superscript> ) implanted into the infarct area at 3 h after AMI], and group 5 (AMI + ECSW-SVF). In vitro, SVF protected H9C2 cells against menadione-induced mitochondrial damage and increased fluorescent intensity of mitochondria in nuclei ( p < 0.01). By day 42 after AMI, LVEF was highest in group 1, lowest in group 2, significantly higher in group 5 than in groups 3 and 4, and similar between the latter two groups (all p < 0.0001). LV remodeling and infarcted, fibrotic, and collagen deposition areas as well as apoptotic nuclei exhibited an opposite pattern to LVEF among the groups (all p < 0.0001). Protein expressions of CD31/vWF/eNOS/PGC-1 α / α -MHC/mitochondrial cytochrome C exhibited an identical pattern, whilst protein expressions of MMP-9/TNF- α /IL-1 β /NF- κ B/caspase-3/PARP/Samd3/TGF- β /NOX-1/NOX-2/oxidized protein/ β -MHC/BNP exhibited an opposite pattern to LVEF among five groups (all p < 0.0001). Cellular expressions of CXCR4/SDF-1 α /Sca-1/c-Kit significantly and progressively increased from groups 1 to 5 (all p < 0.0001). Cellular expression of γ -H2AX/CD68 displayed an opposite pattern to LVEF among the five groups (all p < 0.0001). In conclusion, ECSW-SVF therapy effectively preserved LVEF and inhibited LV remodeling in rat AMI.
- Subjects :
- Animals
Apoptosis drug effects
Biomarkers metabolism
Cell Line
Cell Nucleus drug effects
Cell Nucleus metabolism
Coculture Techniques
Collagen metabolism
DNA Damage
Echocardiography
Endothelial Progenitor Cells drug effects
Endothelial Progenitor Cells metabolism
Male
Mesenchymal Stem Cells drug effects
Mesenchymal Stem Cells metabolism
Mitochondrial Dynamics drug effects
Myocardial Infarction diagnostic imaging
Myocardial Infarction pathology
Oxidative Stress drug effects
Rats, Sprague-Dawley
Stromal Cells drug effects
Stromal Cells metabolism
Time Factors
Tumor Suppressor p53-Binding Protein 1 metabolism
Ventricular Function, Left drug effects
Ventricular Remodeling drug effects
Vitamin K 3 pharmacology
X-ray Repair Cross Complementing Protein 1 metabolism
Adipose Tissue metabolism
Extracorporeal Shockwave Therapy
Myocardial Infarction physiopathology
Ventricular Function, Left physiology
Ventricular Remodeling physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1942-0994
- Volume :
- 2018
- Database :
- MEDLINE
- Journal :
- Oxidative medicine and cellular longevity
- Publication Type :
- Academic Journal
- Accession number :
- 30416645
- Full Text :
- https://doi.org/10.1155/2018/7518920