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3 results on '"Philippe Douzenel"'

1. Antioxidant and antibacterial activity of solid-liquid and enzyme-assisted extraction of phenolic compound from three species of tropical Sargassum

Author

Gilles Bedoux, M Deniel, Maya Puspita, Ita Widowati, Nathalie Bourgougnon, Ocky Karna Radjasa, Philippe Douzenel, Diponegoro University, Ecogéochimie et Fonctionnement des Ecosystèmes Benthiques (EFEB), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Institut de Recherche Dupuy de Lôme (IRDL), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS), Université de Bretagne Sud (UBS), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Centre National de la Recherche Scientifique (CNRS), and Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Université de Bretagne Sud (UBS)

Subjects
0106 biological sciences, chemistry.chemical_classification, Antioxidant, Chromatography, biology, Chemistry, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Extraction (chemistry), 04 agricultural and veterinary sciences, biology.organism_classification, 040401 food science, 01 natural sciences, 0404 agricultural biotechnology, Enzyme, [SDU]Sciences of the Universe [physics], 010608 biotechnology, Sargassum, medicine, [CHIM]Chemical Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Antibacterial activity, Solid liquid, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017
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2. Assessment and monitoring of water quality of the gulf of Morbihan, a littoral ecosystem under high anthropic pressure

Author

Mikaël Kedzierski, V. Le Tilly, Olivier Sire, Hélène Duval, Valérie Maxime, A. Le Grand, Philippe Douzenel, Université de Bretagne Sud (UBS), Institut de Recherche Dupuy de Lôme (IRDL), Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Université de Bretagne Sud (UBS), ASPEET grant from the Universite Bretagne Sud, SERPBIO foundation, city of Locmariaquer, and AQUASTER grant from Region Bretagne

Subjects
0301 basic medicine, Pollution, Estrogenic endocrine disrupters, media_common.quotation_subject, Context (language use), Saccharomyces cerevisiae, 010501 environmental sciences, Aquatic Science, Endocrine Disruptors, Enzyme response index, Oceanography, 01 natural sciences, 03 medical and health sciences, [SPI]Engineering Sciences [physics], Water Quality, Littoral zone, Animals, Ecosystem, 14. Life underwater, Crassostrea, Littoral water monitoring, 0105 earth and related environmental sciences, media_common, Glutathione Transferase, Hydrology, biology, Ecology, Filter feeder, Contamination, Gulf of Morbihan, biology.organism_classification, 030104 developmental biology, Crassostrea gigas, Acetylcholinesterase, Environmental science, Biological Assay, Water quality, France, Environmental Monitoring
Abstract

International audience; This field study is intended to propose a global methodology to assess and monitor the water quality of the gulf of Morbihan, a littoral ecosystem under increasing anthropic pressure. To this end, the Locmariaquer site, where Crassostrea gigas is extensively cultivated, was selected to perform a one-year follow-up of tissular glutathione S-transferase and acetylcholinesterase specific activities in this filter feeder organism. Calculation of an integrated index, corresponding to the ratio of the two enzymes activities, allowed to discriminate from the environmental noise, several clusters which could be representative environmental stress, potentially latent pollution. Moreover, the estrogenic activity was assessed in water samples collected at Locmariaquer and other strategic sites of the gulf. The results evidenced a low estrogenic-disrupting compound contamination of waters. Overall, this methodology produced an accurate outlook of a basal state for the gulf and could be developed in the context of a chronic monitoring of this site.

Published
2017
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3. Antifouling activity of macroalgal extracts on Fragilaria pinnata (Bacillariophyceae): a comparison with Diuron

Author

Jean-Luc Mouget, Alexandra Bazes, Philippe Douzenel, Florence Vouvé, Alla Silkina, Véronique Le Tilly, Nathalie Bourgougnon, Laboratoire d'Ingénierie des Matériaux de Bretagne (LIMATB), Université de Bretagne Sud (UBS)-Institut Brestois du Numérique et des Mathématiques (IBNM), and Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO)

Subjects
0106 biological sciences, Biocide, Biofouling, Health, Toxicology and Mutagenesis, 010501 environmental sciences, Aquatic Science, 01 natural sciences, Risk Assessment, chemistry.chemical_compound, Botany, Paint, Fucoxanthin, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, 14. Life underwater, Sargassum muticum, Ships, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, Diatoms, Biological Products, biology, Herbicides, Pigmentation, 010604 marine biology & hydrobiology, Sargassum, Diadinoxanthin, Diatoxanthin, Eukaryota, Photosystem II Protein Complex, biology.organism_classification, Spectrometry, Fluorescence, chemistry, Diuron, Rhodophyta, Tributyltin, Trialkyltin Compounds, Disinfectants
Abstract

The tributyltin-based products and organic biocides which are incorporated into antifouling paints have had a negative impact on the marine environment, and the ban on tributyltin-based antifouling products has urged the industry to find substitutes to prevent the development of fouling on ship hulls. Natural antifouling agents could be isolated from marine resources, providing an alternative option for the industry. The effects of different marine seaweed extracts from Sargassum muticum and Ceramium botryocarpum on the growth, pigment content and photosynthetic apparatus of the marine diatom Fragilaria pinnata were compared with those of Diuron, a biocide widely used in antifouling paints. The addition of the macroalgal extracts in the culture medium resulted in an inhibition of the growth of F. pinnata, but this inhibition was lower than that obtained with Diuron. After transfer to a biocide-free medium, F. pinnata cells previously exposed to the macroalgal extracts exhibited normal growth, in contrast to Diuron-treated cells, which died, demonstrating that the effects of the natural antifouling agents were reversible. Macroalgal extracts and Diuron-induced modifications in F. pinnata cellular pigment content. Chlorophyll a, fucoxanthin, and the xanthophyll pool, diadinoxanthin and diatoxanthin, were the most affected. Changes in the structure and function of the photosynthetic apparatus were studied by microspectrofluorimetry, and provided a comprehensive evaluation of the inhibition of the diatom Photosystem II (PSII) by the biocides. This study confirms that natural extracts from the macroalgae studied have the potential to be used as a substitute to commercial biocides in antifouling paints.

Published
2009

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