1. Reconstruction of the sialylation pathway in the ancestor of eukaryotes
- Author
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Petit, Daniel, Teppa, Elin, Cenci, Ugo, Ball, Steven, Harduin-Lepers, Anne, Institut Pprime (PPRIME), Université de Poitiers-ENSMA-Centre National de la Recherche Scientifique (CNRS), Fundación Instituto Leloir, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS)-ENSMA, Fundación Instituto Leloir [Buenos Aires], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Limoges (UNILIM), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), CNRS, Université de Lille, PEIRENE [PEIRENE], Instituto de Investigaciones Bioquímicas [Buenos Aires] [IIBBA], and Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
- Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Otras Ciencias Biológicas ,lcsh:Medicine ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,sialyltransferases ,Last eukaryotes Common Ancestor ,[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy ,GT29 ,Article ,Ciencias Biológicas ,purl.org/becyt/ford/1 [https] ,sialylation pathway ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Amino Acid Sequence ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,purl.org/becyt/ford/1.6 [https] ,lcsh:Science ,Phylogeny ,ComputingMilieux_MISCELLANEOUS ,[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] ,lcsh:R ,Computational Biology ,Eukaryota ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,[SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN] ,[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] ,N-Acetylneuraminic Acid ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM] ,lcsh:Q ,Protein Processing, Post-Translational ,CIENCIAS NATURALES Y EXACTAS - Abstract
The biosynthesis of sialylated molecules of crucial relevance for eukaryotic cell life is achieved by sialyltransferases (ST) of the CAZy family GT29. These enzymes are widespread in the Deuterostoma lineages and more rarely described in Protostoma, Viridiplantae and various protist lineages raising the question of their presence in the Last eukaryotes Common Ancestor (LECA). If so, it is expected that the main enzymes associated with sialic acids metabolism are also present in protists. We conducted phylogenomic and protein sequence analyses to gain insights into the origin and ancient evolution of ST and sialic acid pathway in eukaryotes, Bacteria and Archaea. Our study uncovered the unreported occurrence of bacterial GT29 ST and evidenced the existence of 2 ST groups in the LECA, likely originating from the endosymbiotic event that generated mitochondria. Furthermore, distribution of the major actors of the sialic acid pathway in the different eukaryotic phyla indicated that these were already present in the LECA, which could also access to this essential monosaccharide either endogenously or via a sialin/sialidase uptake mechanism involving vesicles. This pathway was lost in several basal eukaryotic lineages including Archaeplastida despite the presence of two different ST groups likely assigned to other functions. Fil: Petit, Daniel. Université de Limoges; Francia Fil: Teppa, Roxana Elin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Cenci, Ugo. Universite Lille; Francia Fil: Ball, Steven. Universite Lille; Francia Fil: Harduin Lepers, Anne. Universite Lille; Francia
- Published
- 2018
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