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5 results on '"Migliorini, Elisa"'

1. Citation

Author

Cavalcanti-Adam, Elisabetta Ada, Migliorini, Elisa, Laboratoire des matériaux et du génie physique (LMGP ), and Institut National Polytechnique de Grenoble (INPG)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, [CHIM.POLY]Chemical Sciences/Polymers, [SDV]Life Sciences [q-bio], [SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

2. Differential bioactivity of four BMP-family members as function of biomaterial stiffness

Author

Khodr, Paul Machillot, Guevara Garcia A, Laure Fourel, Albiges Rizo C, Sales Ramos A, Catherine Picart, Elisa Migliorini, Biomimétisme et Médecine Régénératrice (BRM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-BioSanté (UMR BioSanté), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Laboratoire des matériaux et du génie physique (LMGP ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), ANR-17-CE13-0022,CODECIDE,Coincidence de detection dans l'identité cellulaire(2017), ANR-19-CE13-0031,GlyCON,Décrypter la base biophysique par laquelle les glycosaminoglycanes contrôlent la signalisation des facteurs de croissance pendant le développement : une approche biomimétique(2019), European Project: 259370,EC:FP7:ERC,ERC-2010-StG_20091028,BIOMIM(2011), European Project: 692924,H2020,ERC-2015-PoC,BIOACTIVECOATINGS(2016), Migliorini, Elisa, Coincidence de detection dans l'identité cellulaire - - CODECIDE2017 - ANR-17-CE13-0022 - AAPG2017 - VALID, Décrypter la base biophysique par laquelle les glycosaminoglycanes contrôlent la signalisation des facteurs de croissance pendant le développement : une approche biomimétique - Décrypter la base biophysique par laquelle les glycosaminoglycanes contrôlent la signalisation des facteurs de croissance pendant le développement : une approche biomimétique - - GlyCON2019 - ANR-19-CE13-0031 - AAPG2019 - VALID, Biomimetic films and membranes as advanced materials for studies on cellular processes - BIOMIM - - EC:FP7:ERC2011-06-01 - 2016-05-31 - 259370 - VALID, and BioActive Coatings in multi-well cell culture plates - BIOACTIVECOATINGS - - H20202016-04-01 - 2017-09-30 - 692924 - VALID

Subjects
animal structures, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Integrin, Biophysics, Bioengineering, Biocompatible Materials, Smad Proteins, SMAD, Article, Biomaterials, 03 medical and health sciences, 0302 clinical medicine, Transforming Growth Factor beta, Progenitor cell, Cell adhesion, Beta (finance), [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, 030304 developmental biology, 0303 health sciences, biology, Chemistry, 030302 biochemistry & molecular biology, Cell Differentiation, Bone Morphogenetic Protein Receptors, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, Cell biology, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, Mechanics of Materials, 030220 oncology & carcinogenesis, Bone Morphogenetic Proteins, embryonic structures, biology.protein, Ceramics and Composites, Phosphorylation, Alkaline phosphatase, Function (biology), Signal Transduction
Abstract

International audience; While a soft film itself is not able to induce cell spreading, BMP-2 presented via such soft film (so called“matrix-bound BMP-2”) was previously shown to trigger cell spreading, migration and downstream BMP-2signaling. Here, we used thin films of controlled stiffness presenting matrix-bound BMPs to study the effectof four BMP members (BMP-2, 4, 7, 9) on cell adhesion and differentiation of skeletal progenitors. Weperformed automated high content screening of cellular responses, including cell number, cell spreading area,SMAD phosphorylation and alkaline phosphatase activity. We revealed that the cell response to bBMPs isBMP-type specific and involved certain BMP receptors and beta chain integrins. In addition, this response isstiffness-dependent for several receptors. The basolateral presentation of the BMPs allowed us to discriminatethe specificity of cellular response and the role of type I and II BMP receptors and of β integrins in a BMPtypeand stiffness-dependent manner. Notably, the role of BMP-2 and BMP-4 were found to have distinctroles, while ALK5, previously known as a TGF-β receptor was revealed to be involved in the BMP-pathway.

Published
2022

3. 3D-printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect

Author

Jean Boutonnat, Catherine Picart, Charlotte Garot, Sanela Morand, Georges Bettega, Vincent Fitzpatrick, Julien Vollaire, Paul Machillot, Véronique Josserand, M. Bouyer, Biomimétisme et Médecine Régénératrice (BRM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-BioSanté (UMR BioSanté), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Laboratoire des matériaux et du génie physique (LMGP ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA), Hôpital la Tronche, ANR-18-CE17-0016,OBOE,Régénération de défaut osseux induite par la surface d'un biomatériau(2018), European Project: 259370,EC:FP7:ERC,ERC-2010-StG_20091028,BIOMIM(2011), Migliorini, Elisa, APPEL À PROJETS GÉNÉRIQUE 2018 - Régénération de défaut osseux induite par la surface d'un biomatériau - - OBOE2018 - ANR-18-CE17-0016 - AAPG2018 - VALID, and Biomimetic films and membranes as advanced materials for studies on cellular processes - BIOMIM - - EC:FP7:ERC2011-06-01 - 2016-05-31 - 259370 - VALID

Subjects
Medicine (General), 3d printed, Scaffold, Materials science, QH301-705.5, Biomedical Engineering, Repair Kinetics, Bioengineering, 02 engineering and technology, Bone healing, Biomaterials, 03 medical and health sciences, chemistry.chemical_compound, R5-920, Polylactic acid, Tissue engineering, Full Length Article, Bone morphogenetic protein 2 (BMP-2), Biology (General), Bone regeneration, Molecular Biology, 030304 developmental biology, [SDV.IB] Life Sciences [q-bio]/Bioengineering, 0303 health sciences, 3D printing, Cell Biology, 021001 nanoscience & nanotechnology, Bone defect, Critical-size bone defect, 3. Good health, chemistry, [SDV.IB]Life Sciences [q-bio]/Bioengineering, 0210 nano-technology, Biotechnology, Biomedical engineering
Abstract

The reconstruction of large bone defects (12 cm3) remains a challenge for clinicians. We developed a new critical-size mandibular bone defect model on a minipig, close to human clinical issues. We analyzed the bone reconstruction obtained by a 3D-printed scaffold made of clinical-grade polylactic acid (PLA), coated with a polyelectrolyte film delivering an osteogenic bioactive molecule (BMP-2). We compared the results (computed tomography scans, microcomputed tomography scans, histology) to the gold standard solution, bone autograft. We demonstrated that the dose of BMP-2 delivered from the scaffold significantly influenced the amount of regenerated bone and the repair kinetics, with a clear BMP-2 dose-dependence. Bone was homogeneously formed inside the scaffold without ectopic bone formation. The bone repair was as good as for the bone autograft. The BMP-2 doses applied in our study were reduced 20- to 75-fold compared to the commercial collagen sponges used in the current clinical applications, without any adverse effects. Three-dimensional printed PLA scaffolds loaded with reduced doses of BMP-2 may be a safe and simple solution for large bone defects faced in the clinic., Graphical abstract Image 1, Highlights • We created a critical-size bone defect model in the mandible of mature minipigs, close to multiple clinical applications. • We engineered a new 3D-printed scaffold made of clinical-grade PLA and manufactured by FDM to fill the defect. • The BMP-2 dose released from the implants significantly influenced the amount of regenerated bone and the repair kinetics. • Bone reconstructed using this osteoinductive implant was homogeneous. • 3D-printed PLA scaffolds coated with 2D osteoinductive coatings lead to similar bone regeneration as bone autograft.

Published
2021

4. Additive Manufacturing of Material Scaffolds for Bone Regeneration: Toward Application in the Clinics

Author

Catherine Picart, Charlotte Garot, Georges Bettega, Biomimétisme et Médecine Régénératrice (BRM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-BioSanté (UMR BioSanté), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), ANR-18-CE17-0016,OBOE,Régénération de défaut osseux induite par la surface d'un biomatériau(2018), European Project: 259370,EC:FP7:ERC,ERC-2010-StG_20091028,BIOMIM(2011), Migliorini, Elisa, APPEL À PROJETS GÉNÉRIQUE 2018 - Régénération de défaut osseux induite par la surface d'un biomatériau - - OBOE2018 - ANR-18-CE17-0016 - AAPG2018 - VALID, and Biomimetic films and membranes as advanced materials for studies on cellular processes - BIOMIM - - EC:FP7:ERC2011-06-01 - 2016-05-31 - 259370 - VALID

Subjects
Pore size, [SDV.IB] Life Sciences [q-bio]/Bioengineering, Materials science, Material type, business.industry, 0206 medical engineering, Implantation Site, 3D printing, 02 engineering and technology, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 020601 biomedical engineering, Article, Bone tissue engineering, Electronic, Optical and Magnetic Materials, Biomaterials, Tissue engineering, 3d image, Electrochemistry, [SDV.IB]Life Sciences [q-bio]/Bioengineering, 0210 nano-technology, Bone regeneration, business, ComputingMilieux_MISCELLANEOUS, Biomedical engineering
Abstract

Additive manufacturing (AM) allows the fabrication of customized bone scaffolds in terms of shape, pore size, material type and mechanical properties. Combined with the possibility to obtain a precise 3D image of the bone defects using computed tomography or magnetic resonance imaging, it is now possible to manufacture implants for patient-specific bone regeneration. This paper reviews the state-of-the-art of the different materials and AM techniques used for the fabrication of 3D-printed scaffolds in the field of bone tissue engineering. Their advantages and drawbacks are highlighted. For materials, specific criteria, were extracted from a literature study: biomimetism to native bone, mechanical properties, biodegradability, ability to be imaged (implantation and follow-up period), histological performances and sterilization process. AM techniques can be classified in three major categories: extrusion-based, powder-based and liquid-base. Their price, ease of use and space requirement are analyzed. Different combinations of materials/AM techniques appear to be the most relevant depending on the targeted clinical applications (implantation site, presence of mechanical constraints, temporary or permanent implant). Finally, some barriers impeding the translation to human clinics are identified, notably the sterilization process.

Published
2021

5. Learning from BMPs and their biophysical extracellular matrix microenvironment for biomaterial design

Author

Corinne Albiges-Rizo, Elisa Migliorini, Amaris Guevara-Garcia, Catherine Picart, Biomimétisme et Médecine Régénératrice (BRM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-BioSanté (UMR BioSanté), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Laboratoire des matériaux et du génie physique (LMGP ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), ANR-17-CE13-0022,CODECIDE,Coincidence de detection dans l'identité cellulaire(2017), ANR-19-CE13-0031,GlyCON,Décrypter la base biophysique par laquelle les glycosaminoglycanes contrôlent la signalisation des facteurs de croissance pendant le développement : une approche biomimétique(2019), European Project: 259370,EC:FP7:ERC,ERC-2010-StG_20091028,BIOMIM(2011), European Project: 334966,EC:FP7:ERC,ERC-2012-PoC,OSCODI(2013), European Project: 692924,H2020,ERC-2015-PoC,BIOACTIVECOATINGS(2016), Biomimetism and regenerative medicine (BRM), Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire [Grenoble] (CHU)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Migliorini, Elisa, Coincidence de detection dans l'identité cellulaire - - CODECIDE2017 - ANR-17-CE13-0022 - AAPG2017 - VALID, Décrypter la base biophysique par laquelle les glycosaminoglycanes contrôlent la signalisation des facteurs de croissance pendant le développement : une approche biomimétique - Décrypter la base biophysique par laquelle les glycosaminoglycanes contrôlent la signalisation des facteurs de croissance pendant le développement : une approche biomimétique - - GlyCON2019 - ANR-19-CE13-0031 - AAPG2019 - VALID, Biomimetic films and membranes as advanced materials for studies on cellular processes - BIOMIM - - EC:FP7:ERC2011-06-01 - 2016-05-31 - 259370 - VALID, Osteo-inductive coating of orthopedic and dental implants - OSCODI - - EC:FP7:ERC2013-07-01 - 2014-12-31 - 334966 - VALID, and BioActive Coatings in multi-well cell culture plates - BIOACTIVECOATINGS - - H20202016-04-01 - 2017-09-30 - 692924 - VALID

Subjects
0301 basic medicine, Histology, animal structures, Biomaterial design, Physiology, Endocrinology, Diabetes and Metabolism, media_common.quotation_subject, [SDV]Life Sciences [q-bio], BMPs, 030209 endocrinology & metabolism, Biocompatible Materials, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Article, Extracellular matrix, 03 medical and health sciences, [SPI]Engineering Sciences [physics], 0302 clinical medicine, Bmp signaling, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Animals, Humans, Spatial localization, Mechanotransduction, Bone regeneration, Internalization, ComputingMilieux_MISCELLANEOUS, media_common, mechanotransduction, Extracellular Matrix Proteins, Chemistry, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Cell biology, 030104 developmental biology, bioinspiration, Bone Morphogenetic Proteins, embryonic structures, Bioinspiration, Signal Transduction, biomaterials
Abstract

International audience; It is nowadays well-accepted that the extracellular matrix (ECM) is not a simple reservoir for growth factors but is an organization center of their biological activity. In this review, we focus on the ability of the ECM to regulate the biological activity of BMPs. In particular, we survey the role of the ECM components, notably the glycosaminoglycans and fibrillary ECM proteins, which can be promoters or repressors of the biological activities mediated by the BMPs. We examine how a process called mechano-transduction induced by the ECM can affect BMP signaling, including BMP internalization by the cells. We also focus on the spatio-temporal regulation of the BMPs, including their release from the ECM, which enables to modulate their spatial localization as well as their local concentration. We highlight how biomaterials can recapitulate some aspects of the BMPs/ECM interactions and help to answer fundamental questions to reveal previously unknown molecular mechanisms. Finally, the design of new biomaterials inspired by the ECM to better present BMPs is discussed, and their use for a more efficient bone regeneration in vivo is also highlighted.

Published
2020

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