1. An immunoproteomic approach revealed antigenic proteins enhancing serodiagnosis performance of bird fancier's lung
- Author
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Gabriel Reboux, Jean-Charles Dalphin, Adeline Rouzet, Benoît Valot, Laurence Millon, Thierry Balliau, Sandrine Roussel, Anne Gondouin, Paul De Vuyst, Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Service de parasitologie et mycologie [CHU de Besançon], Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon), Service de Pneumologie [Besançon], Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Hôpital Jean Minjoz-Université de Franche-Comté (UFC), Erasme Hospital, Brussels, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Besancon University Hospital [2015-A01803-46], Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ), Service de Pneumologie, Hôpital Jean-Minjoz, Centre Hospitalier Universitaire de Besançon, Université de Franche-Comté, Besançon, France., Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) ( GQE-Le Moulon ), Institut National de la Recherche Agronomique ( INRA ) -Université Paris-Sud - Paris 11 ( UP11 ) -AgroParisTech-Centre National de la Recherche Scientifique ( CNRS ), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Hôpital Erasme [Bruxelles] (ULB), Faculté de Médecine [Bruxelles] (ULB), and Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB)
- Subjects
Male ,Proteomics ,MESH : Aged ,MESH: Immunoelectrophoresis ,MESH : Chromatography, Liquid ,law.invention ,Feces ,MESH: Enzyme Precursors ,0302 clinical medicine ,Tandem Mass Spectrometry ,MESH : Bird Fancier's Lung ,Electrophoresis, Gel, Two-Dimensional ,MESH: Animals ,MESH: Bird Fancier's Lung ,ComputingMilieux_MISCELLANEOUS ,Aged, 80 and over ,Electrophoresis, Agar Gel ,MESH: Middle Aged ,MESH: Proteomics ,MESH: Feces ,Precipitin ,MESH : Enzyme-Linked Immunosorbent Assay ,MESH: Case-Control Studies ,3. Good health ,MESH: Reproducibility of Results ,[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Area Under Curve ,Recombinant DNA ,Antibody ,2D electrophoresis ,MESH : Case-Control Studies ,Immunology ,[ SDV.EE.SANT ] Life Sciences [q-bio]/Ecology, environment/Health ,Avian Proteins ,03 medical and health sciences ,Antigen ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Humans ,Serologic Tests ,MESH : Middle Aged ,MESH : Predictive Value of Tests ,MESH : Birds ,MESH : Aged, 80 and over ,Aged ,MESH: Humans ,MESH : Reproducibility of Results ,MESH : Humans ,MESH: Serologic Tests ,MESH : Immunoglobulin Light Chains, Surrogate ,MESH: Tandem Mass Spectrometry ,MESH: Adult ,MESH : Electrophoresis, Gel, Two-Dimensional ,Bird fancier's lung ,Allergens ,MESH: ROC Curve ,medicine.disease ,MESH: Electrophoresis, Gel, Two-Dimensional ,030104 developmental biology ,030228 respiratory system ,Case-Control Studies ,MESH : Inhalation Exposure ,MESH: Chromatography, Liquid ,MESH: Female ,[ SDV.MHEP.PSR ] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract ,0301 basic medicine ,Immunoelectrophoresis ,[SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract ,Recombinant antigens ,Serology ,MESH : Proteomics ,MESH: Aged, 80 and over ,MESH: Avian Proteins ,law ,Immunology and Allergy ,MESH : Female ,MESH: Endopeptidases ,MESH : Allergens ,MESH: Aged ,Enzyme Precursors ,Inhalation Exposure ,Serodiagnosis ,medicine.diagnostic_test ,MESH : Immunoelectrophoresis ,MESH : Enzyme Precursors ,MESH : Avian Proteins ,MESH: Enzyme-Linked Immunosorbent Assay ,Middle Aged ,MESH : Adult ,MESH : Endopeptidases ,MESH: Predictive Value of Tests ,MESH : Serologic Tests ,MESH: Birds ,Female ,ELISA ,MESH : Tandem Mass Spectrometry ,MESH: Inhalation Exposure ,Adult ,MESH: Allergens ,Immunoglobulin Light Chains, Surrogate ,MESH : Male ,Enzyme-Linked Immunosorbent Assay ,Biology ,Birds ,Predictive Value of Tests ,Endopeptidases ,medicine ,Animals ,[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health ,Two-dimensional gel electrophoresis ,Reproducibility of Results ,MESH : Feces ,MESH: Immunoglobulin Light Chains, Surrogate ,MESH: Male ,MESH : Electrophoresis, Agar Gel ,ROC Curve ,biology.protein ,MESH: Area Under Curve ,MESH : Animals ,MESH : Area Under Curve ,MESH: Electrophoresis, Agar Gel ,MESH : ROC Curve ,Hypersensitivity pneumonitis ,Chromatography, Liquid - Abstract
Background Bird fancier's lung (BFL) caused by repeated inhalation of avian proteins is the most common form of hypersensitivity pneumonitis. However, the exact identification of proteins involved is unknown, and serological test use for diagnosis need to be standardized. The objectives of this study were (i) to identify antigenic proteins from pigeon droppings (ii) to provide information about their location in avian matrices and (iii) to produce them in recombinant proteins to evaluate their diagnostic performances. Method Antigenic proteins of pigeon dropping extracts were investigated using 2-dimensional immunoblotting with sera from patients with BFL, asymptomatic exposed controls and healthy volunteers. We investigated the origin of these antigenic proteins by analyzing droppings, blooms and sera using a shotgun proteomic analysis. BFL-associated proteins were produced as recombinant antigens in E. coli and were assessed in ELISA with sera from patients (n = 25) and subject exposed controls (n = 30). These diagnostic performances were compared with those obtained by precipitin techniques (agar gel double diffusion, immunoelectrophoresis). Results We identified 14 antigenic proteins mainly located in droppings and blooms. These proteins were involved in either the digestive or immune systems of pigeons. Using the recombinant BFL-associated proteins: Immunoglobulin lambda-like polypeptide-1 (IGLL1: sensitivity: 76%; specificity: 100%; AUC: 0.93) and Proproteinase E (ProE: sensitivity: 84%; specificity: 80%; AUC: 0.85), the ELISA test showed better performance than precipitin assays with pigeon dropping extracts (sensitivity: 60%; specificity: 93.3%; AUC: 0.76). Conclusion IGLL1 and ProE were identified as the biomarkers of the disease. The use of these highly standardized antigens discriminates BFL cases from exposed subjects in serological assays. The results of this study offer new possibilities for the serological diagnosis of the disease. Clinical trial registration: ClinicalTrials.gov : Identifier NCT03056404 .
- Published
- 2017