1. Expression of renal vitamin D receptors and metabolizing enzymes in IgA nephropathy.
- Author
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Arapović A, Vukojević K, Glavina Durdov M, Benzon B, Šolić I, Racetin A, Jurić M, Čujić T, Kosović I, Lozić M, Borić Škaro D, Ljutić D, Saraga-Babić M, Simičić Majce A, Saraga M, and Filipović N
- Subjects
- Adolescent, Adult, Biopsy, Calcitriol metabolism, Child, Child, Preschool, Female, Humans, Male, Microscopy, Fluorescence, Middle Aged, Nephrectomy, Receptors, Calcitriol metabolism, Vitamin D metabolism, Young Adult, Carcinoma, Renal Cell diagnostic imaging, Gene Expression Regulation, Glomerulonephritis, IGA metabolism, Kidney diagnostic imaging, Kidney metabolism, Kidney Failure, Chronic metabolism, Vitamin D3 24-Hydroxylase metabolism
- Abstract
Aim: One of the main causes of end-stage renal disease (ESRD) in the world is IgA nephropathy (IgAN). Since kidney is a key player in vitamin D metabolism, we investigated the expression of renal vitamin D receptors (VDR) and metabolizing enzymes in IgA nephropathy patients (IgAN-P)., Methods: The sample included twelve IgAN-P who underwent ultrasound-guided renal biopsies and five controls who underwent nephrectomy due to clear renal carcinoma. Immunofluorescent staining was used to determine the expression of VDR, 25-hydroxyvitamin D3 -alpha-hydroxylase (1alpha-OHase) and vitamin D3 24-hydroxylase (CYP24A1)., Results: Significant increase in expression of VDR, which was prominent in distal tubular cells (DTCs) in tissues from IgAN-P, was found in comparison to the controls (p = 0.0368). The expression of 1alpha-OHase, calcitriol synthesizing enzyme, was significantly lower in IgAN-P, in comparison with controls (p < 0.0001). The opposite, expression of CYP24A1 (vitamin D degrading enzyme), was significantly higher in IgAN-P in comparison with controls (p = 0.0003). Additionally, we found significant negative correlation between percentage of CYP24A1 immunoreactive nuclei in proximal tubular cells (PTCs) and estimated glomerular filtration rate (eGFR) in IgAN-P (r = -0.6139; p = 0.0337)., Conclusions: Our research indicates substantially decreased renal calcitriol production and increased vitamin D degradation in kidneys of IgAN-P, but larger studies are needed to confirm our results., (Copyright © 2021 Elsevier GmbH. All rights reserved.)
- Published
- 2021
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