Back to Search
Start Over
Expression of renal vitamin D receptors and metabolizing enzymes in IgA nephropathy.
- Source :
-
Acta histochemica [Acta Histochem] 2021 Jul; Vol. 123 (5), pp. 151740. Date of Electronic Publication: 2021 Jun 08. - Publication Year :
- 2021
-
Abstract
- Aim: One of the main causes of end-stage renal disease (ESRD) in the world is IgA nephropathy (IgAN). Since kidney is a key player in vitamin D metabolism, we investigated the expression of renal vitamin D receptors (VDR) and metabolizing enzymes in IgA nephropathy patients (IgAN-P).<br />Methods: The sample included twelve IgAN-P who underwent ultrasound-guided renal biopsies and five controls who underwent nephrectomy due to clear renal carcinoma. Immunofluorescent staining was used to determine the expression of VDR, 25-hydroxyvitamin D3 -alpha-hydroxylase (1alpha-OHase) and vitamin D3 24-hydroxylase (CYP24A1).<br />Results: Significant increase in expression of VDR, which was prominent in distal tubular cells (DTCs) in tissues from IgAN-P, was found in comparison to the controls (p = 0.0368). The expression of 1alpha-OHase, calcitriol synthesizing enzyme, was significantly lower in IgAN-P, in comparison with controls (p < 0.0001). The opposite, expression of CYP24A1 (vitamin D degrading enzyme), was significantly higher in IgAN-P in comparison with controls (p = 0.0003). Additionally, we found significant negative correlation between percentage of CYP24A1 immunoreactive nuclei in proximal tubular cells (PTCs) and estimated glomerular filtration rate (eGFR) in IgAN-P (r = -0.6139; p = 0.0337).<br />Conclusions: Our research indicates substantially decreased renal calcitriol production and increased vitamin D degradation in kidneys of IgAN-P, but larger studies are needed to confirm our results.<br /> (Copyright © 2021 Elsevier GmbH. All rights reserved.)
- Subjects :
- Adolescent
Adult
Biopsy
Calcitriol metabolism
Child
Child, Preschool
Female
Humans
Male
Microscopy, Fluorescence
Middle Aged
Nephrectomy
Receptors, Calcitriol metabolism
Vitamin D metabolism
Young Adult
Carcinoma, Renal Cell diagnostic imaging
Gene Expression Regulation
Glomerulonephritis, IGA metabolism
Kidney diagnostic imaging
Kidney metabolism
Kidney Failure, Chronic metabolism
Vitamin D3 24-Hydroxylase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0372
- Volume :
- 123
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Acta histochemica
- Publication Type :
- Academic Journal
- Accession number :
- 34111685
- Full Text :
- https://doi.org/10.1016/j.acthis.2021.151740