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1. Evaluation of the Premi Test Salmonella, a commercial low-density DNA microarray system intended for routine identification and typing of Salmonella enterica.

Author

Wattiau P, Weijers T, Andreoli P, Schliker C, Veken HV, Maas HM, Verbruggen AJ, Heck ME, Wannet WJ, Imberechts H, and Vos P

Subjects
Bacterial Typing Techniques methods, Consumer Product Safety, Food Microbiology, Gene Expression Profiling, Genotype, Humans, Reproducibility of Results, Sensitivity and Specificity, Serotyping, Species Specificity, DNA, Bacterial analysis, Food Contamination analysis, Oligonucleotide Array Sequence Analysis methods, Salmonella enterica isolation & purification
Abstract

A new commercial system based on genetic profiling and aimed at identifying Salmonella enterica serovars was evaluated by comparing its performance with classical serotyping on 443 strains. Within 62 serovars represented, 60 gave unique genetic profiles while 2 were undistinguishable. Results were obtained within 8 h, were reproducible and clear-cut. The system allowed single-tube processing of the samples and required no peculiar technical skill. It showed interesting potential for routine laboratory testing.

Published
2008
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