Evaluation of the Premi Test Salmonella, a commercial low-density DNA microarray system intended for routine identification and typing of Salmonella enterica.

Authors :: Wattiau P
Weijers T
Andreoli P
Schliker C
Veken HV
Maas HM
Verbruggen AJ
Heck ME
Wannet WJ
Imberechts H
Vos P
Source :: International journal of food microbiology [Int J Food Microbiol] 2008 Apr 30; Vol. 123 (3), pp. 293-8. Date of Electronic Publication: 2008 Jan 12.
Publication Year :: 2008
Abstract: A new commercial system based on genetic profiling and aimed at identifying Salmonella enterica serovars was evaluated by comparing its performance with classical serotyping on 443 strains. Within 62 serovars represented, 60 gave unique genetic profiles while 2 were undistinguishable. Results were obtained within 8 h, were reproducible and clear-cut. The system allowed single-tube processing of the samples and required no peculiar technical skill. It showed interesting potential for routine laboratory testing.

Subjects :: Bacterial Typing Techniques methods
Consumer Product Safety
Food Microbiology
Gene Expression Profiling
Genotype
Humans
Reproducibility of Results
Sensitivity and Specificity
Serotyping
Species Specificity
DNA, Bacterial analysis
Food Contamination analysis
Oligonucleotide Array Sequence Analysis methods
Salmonella enterica isolation & purification

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