1. Direct tandem mass spectrometric analysis of amino acids in plasma using fluorous derivatization and monolithic solid-phase purification.
- Author
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Tamashima E, Hayama T, Yoshida H, Imakyure O, Yamaguchi M, and Nohta H
- Subjects
- Animals, Disease Models, Animal, Humans, Maple Syrup Urine Disease blood, Mice, Mice, Mutant Strains, Phenylalanine Hydroxylase genetics, Phenylketonurias blood, Reproducibility of Results, Amino Acids blood, Fatty Acids chemistry, Fluorocarbons chemistry, Solid Phase Extraction methods, Spectrometry, Mass, Electrospray Ionization methods, Tandem Mass Spectrometry methods
- Abstract
In this study, we developed a novel direct tandem mass spectrometric method for rapid and accurate analysis of amino acids utilizing a fluorous derivatization and purification technique. Amino acids were perfluoroalkylated with 2H,2H,3H,3H-perfluoroundecan-1-al in the presence of 2-picoline borane via reductive amination. The derivatives were purified by perfluoroalkyl-modified silica-based monolithic solid-phase extraction (monolithic F-SPE), and directly analyzed by tandem mass spectrometry using electrospray ionization without liquid chromatographic separation. The perfluoroalkyl derivatives could be sufficiently distinguished from non-fluorous compounds, i.e. the biological matrix, due to their fluorous interaction. Thus, rapid and accurate determination of amino acids was accomplished. The method was validated with human plasma samples and applied to the analysis of amino acids in the plasma of mice with maple syrup urine disease or phenylketonuria., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
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