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Direct tandem mass spectrometric analysis of amino acids in plasma using fluorous derivatization and monolithic solid-phase purification.
- Source :
-
Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2015 Nov 10; Vol. 115, pp. 201-7. Date of Electronic Publication: 2015 Jul 17. - Publication Year :
- 2015
-
Abstract
- In this study, we developed a novel direct tandem mass spectrometric method for rapid and accurate analysis of amino acids utilizing a fluorous derivatization and purification technique. Amino acids were perfluoroalkylated with 2H,2H,3H,3H-perfluoroundecan-1-al in the presence of 2-picoline borane via reductive amination. The derivatives were purified by perfluoroalkyl-modified silica-based monolithic solid-phase extraction (monolithic F-SPE), and directly analyzed by tandem mass spectrometry using electrospray ionization without liquid chromatographic separation. The perfluoroalkyl derivatives could be sufficiently distinguished from non-fluorous compounds, i.e. the biological matrix, due to their fluorous interaction. Thus, rapid and accurate determination of amino acids was accomplished. The method was validated with human plasma samples and applied to the analysis of amino acids in the plasma of mice with maple syrup urine disease or phenylketonuria.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Disease Models, Animal
Humans
Maple Syrup Urine Disease blood
Mice
Mice, Mutant Strains
Phenylalanine Hydroxylase genetics
Phenylketonurias blood
Reproducibility of Results
Amino Acids blood
Fatty Acids chemistry
Fluorocarbons chemistry
Solid Phase Extraction methods
Spectrometry, Mass, Electrospray Ionization methods
Tandem Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-264X
- Volume :
- 115
- Database :
- MEDLINE
- Journal :
- Journal of pharmaceutical and biomedical analysis
- Publication Type :
- Academic Journal
- Accession number :
- 26222276
- Full Text :
- https://doi.org/10.1016/j.jpba.2015.07.008