1. FSH mediated cAMP signalling upregulates the expression of Gα subunits in pubertal rat Sertoli cells
- Author
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Alka Gupta, Indrashis Bhattacharya, Bhola Shankar Pradhan, Souvik Sen Sharma, Hironmoy Sarkar, and Subeer S. Majumdar
- Subjects
Male ,endocrine system ,Gs alpha subunit ,G protein ,Gi alpha subunit ,Biophysics ,GTP-Binding Protein alpha Subunits, Gi-Go ,Biochemistry ,chemistry.chemical_compound ,Follicle-stimulating hormone ,GTP-binding protein regulators ,Cyclic AMP ,medicine ,Animals ,Sexual Maturation ,Rats, Wistar ,Spermatogenesis ,Molecular Biology ,Cells, Cultured ,Sertoli Cells ,Forskolin ,Reverse Transcriptase Polymerase Chain Reaction ,Chemistry ,Colforsin ,Cell Biology ,Sertoli cell ,Cell biology ,medicine.anatomical_structure ,Animals, Newborn ,Gene Expression Regulation ,Pertussis Toxin ,Second messenger system ,Receptors, FSH ,Follicle Stimulating Hormone ,hormones, hormone substitutes, and hormone antagonists ,Protein Binding ,Signal Transduction - Abstract
Follicle Stimulating Hormone (FSH) acts via FSH-Receptor (FSH-R) by employing cAMP as the dominant secondary messenger in testicular Sertoli cells (Sc) to support spermatogenesis. Binding of FSH to FSH-R, results the recruitment of the intracellular GTP binding proteins, either stimulatory Gαs or inhibitory Gαi that in turn regulate cAMP production in Sc. The cytosolic concentration of cAMP being generated by FSH-R thereafter critically determines the downstream fate of the FSH signalling. The pleiotropic action of FSH due to differential cAMP output during functional maturation of Sc has been well studied. However, the developmental and cellular regulation of the Gα proteins associated with FSH-R is poorly understood in Sc. In the present study, we report the differential transcriptional modulation of the Gα subunit genes by FSH mediated cAMP signalling in neonatal and pubertal rat Sc. Our data suggested that unlike in neonatal Sc, both the basal and FSH/forskolin induced expression of Gαs, Gαi-1, Gαi-2 and Gαi-3 transcripts was significantly (p
- Published
- 2021