1. Nanocarriers enhance the transdermal bioavailability of resveratrol: In-vitro and in-vivo study
- Author
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Ming-Jun Tsai, Yaw-Syan Fu, Yi-Ping Fang, I-Ju Lu, Pao-Chu Wu, and Yaw-Bin Huang
- Subjects
Skin Absorption ,Biological Availability ,02 engineering and technology ,Resveratrol ,Administration, Cutaneous ,030226 pharmacology & pharmacy ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Colloid and Surface Chemistry ,Drug Stability ,Pulmonary surfactant ,Stilbenes ,Animals ,Particle Size ,Physical and Theoretical Chemistry ,Isopropyl myristate ,Skin ,Skin Tests ,Transdermal ,Drug Carriers ,Chromatography ,Viscosity ,Anti-Inflammatory Agents, Non-Steroidal ,Surfaces and Interfaces ,General Medicine ,Permeation ,021001 nanoscience & nanotechnology ,Nanostructures ,Bioavailability ,chemistry ,Emulsion ,Thermodynamics ,Emulsions ,Nanocarriers ,0210 nano-technology ,Biotechnology - Abstract
The aim of this study was to develop and assess the potential of nanostructured emulsion carriers for resveratrol topical application. Different compositions of resveratrol-loaded nanostructured emulsions were prepared using different types and amounts of surfactants and oily phases (isopropyl myristate and caproyl 90). The produced nanostructured emulsions were within the nanosized range 23.4-422.2nm with low viscosity range 2.15-17.53cps. The transdermal amount and deposition amount in the skin after 24 applications of resveratrol-loaded nanostructured emulsion were significantly increased about 896.2-fold and 10.2-fold respectively, when compared to the drug-saturated solution-treated group. Nanostructured emulsion containing IPM and low amounts of mixed surfactant of Tween80/Span 20 showed highest permeation capacity. In vivo study showed that the plasma concentration of resveratrol could be maintained at high levels for a long time after topical application of drug-loaded nanostructured emulsion. The histological examination demonstrated that the free drug- and drug-loaded nanostructured emulsion demonstrated considerably less irritation than the standard irritation group (0.8% paraformaldehyde-treated). The residual contents of resveratrol in the tested formulations after 3 months of storage at 25°C and 40°C were more than 99.97±3.90%. The results of present work confirm the high potential of nanostructured emulsion as carriers for drug topical application.
- Published
- 2016